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The map label for this gene is legS2

Identifier: 52842389

GI number: 52842389

Start: 2440714

End: 2442540

Strand: Reverse

Name: legS2

Synonym: lpg2176

Alternate gene names: 52842389

Gene position: 2442540-2440714 (Counterclockwise)

Preceding gene: 52842397

Following gene: 52842386

Centisome position: 71.89

GC content: 39.74

Gene sequence:

>1827_bases
TTGTTCGTAAGGATATGTGCAATGTTCGGGTTTGTTTCAGATTTATTAACAGCAGCGGTCAGTTCTTTAGATGAATTATT
ACAAGATACTCCGGCACATCAAATCATATTAGGCACAGCTGCTCTTTATTTTCTTTATAATCAATACCATAACCCATCGA
TTTCCAGGTGGTACCGTAGCCGTAATAACGCCAGTATGAAGCAGCGTATTATTGATAGTGCTTACGCCTTGGCAAAAAAT
TTACCTGGGGTGAATCAGATCATTGAGAAAGAGCTCAATAAAGAACTCTCTTCAACGAGAGAAAAATTGAGAATTCAGCG
TTCCGGTATGACTCTGAGGGAGGAAATACCGGAGGAAGGGCTTTCTCCCCAAGATATTTTGAGTGCATTTGATGTGGATG
TGGAAAAATGTCATTTTGATTTTTTATCCGTAACTAATGACAGCCCTGAGAGGGAGTTTCTTGTTGAAAGAGGGGATGGT
AAAGATTCAGGTGCATTATACGCTATTCACCCTAAGGAACTGACAGAGCTGCTTAAAGAAGTTTATGGGGCAACTGCTCT
CACTAATCCTTTGCATGATAAATGGCCTAGAATTAACGCAATGCAAGCGGAGGTGATTCGCTGGTGTCAAAATTTGTTTC
ATGGTTCAAAAGAATGTTATGGTTTATTAACTCATGGGGGTACGACCAGCATTATTGAGGCGATGGCTGCTTATGTCATT
CGCGCAAGGGCAAAAGGCATTGATTATCCTGAAATTGTCGTTCCTGAAACAGCACATGCTGCATTTAAAAAAGCAGCCGA
ATTAACTGGAGCAATATTAATTACGGTTCCTGTTGATAAAAAAACGGGTGCGGTGAATCCCAAGGTCATGAGTTCCTATA
TTACCAGAAATACTGCTGTAATCGTTGGATCTGCCCCATCTTTCATGAATGGAATTCATGATCCTGTCTCTGAGTTAGGT
CAATTAGCAAAGAAAAAAAATGTTCCTTTTCATGTGGATGCCTGTTTGGGTGGATTTTTAACGGCTTTTTTAGATACTTC
ATCAGAACCTATGGATTTTAGAGTACCTGGAGTGACTTCCATTTCAGCCGATTTGCATAAATATGGCTGTTGTCCTAAAG
GCACCTCTGTTTGTCTTTTTAGTGAGGATTCGCCTGCTTTGTCGGTTTATGCCGCATTAAACTGGTCAGGTGGATTGTAT
GCTACACCAGGAATTCTCGATGGTTCAACCAGTGGGGCTCGGGTTGCAGAGGTCTATGCCACTTTGTCTTACTATGGAAA
AAATAAATATCAGGAAATCGCTAAAAGCATTATCACATTACGCAATGCGATTCAAAAAGAATTAACGACATTACTGGAGG
AAGGCAATGGATTAACCAGTGAGGATATTTATGTTTATGGTAATCCTCAATGGTCCATCCTGGGTTTTAGAAGTAACACT
TGCAATGCTCATTTCATTGCAGATGAATTGGAGAAGCGAGGTTGGAAGCTTAATTTGCTACAAAATCCGGACGGATTTCA
CTTATGTTTGACTCATGTGCACACATTAGTCAAAGGTTTCGAAACGCAATTTATAAAAGATTTAAGAGAGGCGGTGATTG
ATGTTAAAAATTATCCTCCTGGAAAGAAACCAAGCGGTAATGTCAAAGTGTATGGTGCTGTTGGTATGATGCCGATTGAG
CTTCAGCGAGAAATTTGTAAACAATATCAAAAAGCCCGTCTTAATTATACTTCTGCCAGTCCAGGGAGTTTGAGGATATT
TACAAATGTGCCTGTAGAAGAAGATGAAGGACTTAGAAATAGAAAAACAGAGAAATATAAAGTGTAA

Upstream 100 bases:

>100_bases
AGATCATCCGGCTCAAGCAAAATACCAGGGCGTTTTTGTTATACTGACACGTATCGACCGGCCTTTATTTTTTTATGCAA
AGTGACTATTATCCATCATT

Downstream 100 bases:

>100_bases
ACCTTACTCTGAACTATTTTTACTGTAAAAATTACGCGCGTTCCTGTACAGAATTTTTTCTGTCAGCTTCTCATCAAATA
GATCCTGGATTATTTGAATG

Product: sphingosine-1-phosphate lyase I

Products: NA

Alternate protein names: SP-lyase; SPL; Sphingosine-1-phosphate aldolase [H]

Number of amino acids: Translated: 608; Mature: 608

Protein sequence:

>608_residues
MFVRICAMFGFVSDLLTAAVSSLDELLQDTPAHQIILGTAALYFLYNQYHNPSISRWYRSRNNASMKQRIIDSAYALAKN
LPGVNQIIEKELNKELSSTREKLRIQRSGMTLREEIPEEGLSPQDILSAFDVDVEKCHFDFLSVTNDSPEREFLVERGDG
KDSGALYAIHPKELTELLKEVYGATALTNPLHDKWPRINAMQAEVIRWCQNLFHGSKECYGLLTHGGTTSIIEAMAAYVI
RARAKGIDYPEIVVPETAHAAFKKAAELTGAILITVPVDKKTGAVNPKVMSSYITRNTAVIVGSAPSFMNGIHDPVSELG
QLAKKKNVPFHVDACLGGFLTAFLDTSSEPMDFRVPGVTSISADLHKYGCCPKGTSVCLFSEDSPALSVYAALNWSGGLY
ATPGILDGSTSGARVAEVYATLSYYGKNKYQEIAKSIITLRNAIQKELTTLLEEGNGLTSEDIYVYGNPQWSILGFRSNT
CNAHFIADELEKRGWKLNLLQNPDGFHLCLTHVHTLVKGFETQFIKDLREAVIDVKNYPPGKKPSGNVKVYGAVGMMPIE
LQREICKQYQKARLNYTSASPGSLRIFTNVPVEEDEGLRNRKTEKYKV

Sequences:

>Translated_608_residues
MFVRICAMFGFVSDLLTAAVSSLDELLQDTPAHQIILGTAALYFLYNQYHNPSISRWYRSRNNASMKQRIIDSAYALAKN
LPGVNQIIEKELNKELSSTREKLRIQRSGMTLREEIPEEGLSPQDILSAFDVDVEKCHFDFLSVTNDSPEREFLVERGDG
KDSGALYAIHPKELTELLKEVYGATALTNPLHDKWPRINAMQAEVIRWCQNLFHGSKECYGLLTHGGTTSIIEAMAAYVI
RARAKGIDYPEIVVPETAHAAFKKAAELTGAILITVPVDKKTGAVNPKVMSSYITRNTAVIVGSAPSFMNGIHDPVSELG
QLAKKKNVPFHVDACLGGFLTAFLDTSSEPMDFRVPGVTSISADLHKYGCCPKGTSVCLFSEDSPALSVYAALNWSGGLY
ATPGILDGSTSGARVAEVYATLSYYGKNKYQEIAKSIITLRNAIQKELTTLLEEGNGLTSEDIYVYGNPQWSILGFRSNT
CNAHFIADELEKRGWKLNLLQNPDGFHLCLTHVHTLVKGFETQFIKDLREAVIDVKNYPPGKKPSGNVKVYGAVGMMPIE
LQREICKQYQKARLNYTSASPGSLRIFTNVPVEEDEGLRNRKTEKYKV
>Mature_608_residues
MFVRICAMFGFVSDLLTAAVSSLDELLQDTPAHQIILGTAALYFLYNQYHNPSISRWYRSRNNASMKQRIIDSAYALAKN
LPGVNQIIEKELNKELSSTREKLRIQRSGMTLREEIPEEGLSPQDILSAFDVDVEKCHFDFLSVTNDSPEREFLVERGDG
KDSGALYAIHPKELTELLKEVYGATALTNPLHDKWPRINAMQAEVIRWCQNLFHGSKECYGLLTHGGTTSIIEAMAAYVI
RARAKGIDYPEIVVPETAHAAFKKAAELTGAILITVPVDKKTGAVNPKVMSSYITRNTAVIVGSAPSFMNGIHDPVSELG
QLAKKKNVPFHVDACLGGFLTAFLDTSSEPMDFRVPGVTSISADLHKYGCCPKGTSVCLFSEDSPALSVYAALNWSGGLY
ATPGILDGSTSGARVAEVYATLSYYGKNKYQEIAKSIITLRNAIQKELTTLLEEGNGLTSEDIYVYGNPQWSILGFRSNT
CNAHFIADELEKRGWKLNLLQNPDGFHLCLTHVHTLVKGFETQFIKDLREAVIDVKNYPPGKKPSGNVKVYGAVGMMPIE
LQREICKQYQKARLNYTSASPGSLRIFTNVPVEEDEGLRNRKTEKYKV

Specific function: Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Possibly implicated in influencing the macrophage autophagy pathway [H]

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=494, Percent_Identity=36.4372469635627, Blast_Score=295, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI17543922, Length=524, Percent_Identity=34.3511450381679, Blast_Score=278, Evalue=8e-75,
Organism=Caenorhabditis elegans, GI17557272, Length=503, Percent_Identity=32.20675944334, Blast_Score=248, Evalue=7e-66,
Organism=Caenorhabditis elegans, GI25148342, Length=504, Percent_Identity=28.1746031746032, Blast_Score=204, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6320500, Length=488, Percent_Identity=33.6065573770492, Blast_Score=229, Evalue=1e-60,
Organism=Drosophila melanogaster, GI21355963, Length=490, Percent_Identity=36.9387755102041, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24654344, Length=490, Percent_Identity=36.9387755102041, Blast_Score=306, Evalue=2e-83,

Paralogues:

None

Copy number: 3820 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.2.27 [H]

Molecular weight: Translated: 67371; Mature: 67371

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVRICAMFGFVSDLLTAAVSSLDELLQDTPAHQIILGTAALYFLYNQYHNPSISRWYRS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RNNASMKQRIIDSAYALAKNLPGVNQIIEKELNKELSSTREKLRIQRSGMTLREEIPEEG
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
LSPQDILSAFDVDVEKCHFDFLSVTNDSPEREFLVERGDGKDSGALYAIHPKELTELLKE
CCHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHEECCCCCCCCCEEEECHHHHHHHHHH
VYGATALTNPLHDKWPRINAMQAEVIRWCQNLFHGSKECYGLLTHGGTTSIIEAMAAYVI
HHCCHHHCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCHHEEEEECCCHHHHHHHHHHHHH
RARAKGIDYPEIVVPETAHAAFKKAAELTGAILITVPVDKKTGAVNPKVMSSYITRNTAV
HHHHCCCCCCCEECCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEE
IVGSAPSFMNGIHDPVSELGQLAKKKNVPFHVDACLGGFLTAFLDTSSEPMDFRVPGVTS
EEECCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCCEEECCCCCC
ISADLHKYGCCPKGTSVCLFSEDSPALSVYAALNWSGGLYATPGILDGSTSGARVAEVYA
HHHHHHHHCCCCCCCEEEEECCCCCCEEEEEEEECCCCEEECCCCCCCCCCCHHHHHHHH
TLSYYGKNKYQEIAKSIITLRNAIQKELTTLLEEGNGLTSEDIYVYGNPQWSILGFRSNT
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEEECCC
CNAHFIADELEKRGWKLNLLQNPDGFHLCLTHVHTLVKGFETQFIKDLREAVIDVKNYPP
CCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GKKPSGNVKVYGAVGMMPIELQREICKQYQKARLNYTSASPGSLRIFTNVPVEEDEGLRN
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCHH
RKTEKYKV
CCCCCCCC
>Mature Secondary Structure
MFVRICAMFGFVSDLLTAAVSSLDELLQDTPAHQIILGTAALYFLYNQYHNPSISRWYRS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
RNNASMKQRIIDSAYALAKNLPGVNQIIEKELNKELSSTREKLRIQRSGMTLREEIPEEG
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
LSPQDILSAFDVDVEKCHFDFLSVTNDSPEREFLVERGDGKDSGALYAIHPKELTELLKE
CCHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHEECCCCCCCCCEEEECHHHHHHHHHH
VYGATALTNPLHDKWPRINAMQAEVIRWCQNLFHGSKECYGLLTHGGTTSIIEAMAAYVI
HHCCHHHCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCHHEEEEECCCHHHHHHHHHHHHH
RARAKGIDYPEIVVPETAHAAFKKAAELTGAILITVPVDKKTGAVNPKVMSSYITRNTAV
HHHHCCCCCCCEECCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEE
IVGSAPSFMNGIHDPVSELGQLAKKKNVPFHVDACLGGFLTAFLDTSSEPMDFRVPGVTS
EEECCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCCEEECCCCCC
ISADLHKYGCCPKGTSVCLFSEDSPALSVYAALNWSGGLYATPGILDGSTSGARVAEVYA
HHHHHHHHCCCCCCCEEEEECCCCCCEEEEEEEECCCCEEECCCCCCCCCCCHHHHHHHH
TLSYYGKNKYQEIAKSIITLRNAIQKELTTLLEEGNGLTSEDIYVYGNPQWSILGFRSNT
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEEECCC
CNAHFIADELEKRGWKLNLLQNPDGFHLCLTHVHTLVKGFETQFIKDLREAVIDVKNYPP
CCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GKKPSGNVKVYGAVGMMPIELQREICKQYQKARLNYTSASPGSLRIFTNVPVEEDEGLRN
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCHH
RKTEKYKV
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA