Click here to switch to the map view.

The map label for this gene is phrB

Identifier: 52840467

GI number: 52840467

Start: 245097

End: 246512

Strand: Reverse

Name: phrB

Synonym: lpg0212

Alternate gene names: 52840467

Gene position: 246512-245097 (Counterclockwise)

Preceding gene: 52840468

Following gene: 52840466

Centisome position: 7.26

GC content: 41.81

Gene sequence:

>1416_bases
ATGACTATTGCTCTTGTGTGGTTCCGTCAAGATTTAAGATTGACCGATAATCCCGCGTTCATCGAAGCATGCTCCCATCA
TGAAATTGTAATTCCACTTTATATTTATGATGATAAATGCAGTGTTTTAGGGCAAGCTCAAGCTTGGTGGCTCCATTATA
GTCTCATTGCTTTGGGCAAATCGTTAAATCAACAGGGTTTAAGCCTCGTTCTCCGCAAAGGATCTCCGCAAGAGATTATT
TTGGAATTAGTCGCACAGTATGGTGTGGAATCAGTCTATTGGAATCGCTGCTATGAACCACAGGTCATTGCACGCGATAA
AAAAATTAAGCTTGCTTTGGTTGAAAGAGAAATTAAAGTGCAGAGTTTCAATGGAAATTTACTTCATGAACCGTGGACAA
TTAAAAATAAAAATGGGGATTTTTTTAAGGTTTTTACTCCTTACTGGAAACAATGTCGACAAATTCTTAATATCCCCTCG
CCTTTTATTCTCACTAAACGCCCAAGGGGAATCGAAATTGCAAGTGATGACATTGGCAAATGGAAGTTATTGCCAGCAAT
TAATTGGGCAGCACGATTTTCTGAATATTGGATTCCTGGAGAAGAGGGCGCACAAAAAAAACTGGATGAGTTTATTGTTC
ATCACCTTAGCGGATATAAAAGGGATCGCGATTTTCCAGCTAAAAATGCGACATCTCGATTGTCACCTCATTTGCACTTT
GGGGAAATCAGTCCTTGGGTGATTTTAAGAGCACTCGAGTTGGCTAAACTGGAACATACCTGTGATCTGGCCTCCGTAGA
GCATTTTTTATCAGAGCTGGGGTGGAGAGAGTTTTCTGTCTATCTGCTCTACCACTTCCCCAAGCTGGACTGCGAAAATT
TTAGAAAGGAGTTTGATGCATTCCCCTGGCAAAATGATGAGCAATTATTAACTTGCTGGCAAAAGGGAATGACTGGGTAT
CCTATCATTGATGCTGGAATGAGAGAATTATGGGCCACAGGATACATGCACAACCGCGTTCGCATGATTGTCGCATCATT
TCTTACCAAAGATTTACTGATTGATTGGCGTTCGGGCGCACAGTGGTTTTTAGACACCCTAGTCGATGCGGATCTGGCGA
ATAACAGCGCGAGCTGGCAATGGGTAGCAGGATGCGGTGCGGATGCAGCACCTTATTTCCGTATTTTCAATCCTATTTTA
CAAAGCCAAAAATTTGACCCGGATGGCGTTTACATACGCCAATGGATCCCCGAACTTGCCCAATTAAATAGTCAGTCAAT
TCATCAACCCTGGACAGATGCTGACGCCAGTTCCATTTTTTTAGGGAAAAAATATCCCAAACCCATCATTGATCACCAAG
AAGCGAGAACTAAGGCATTGAGTTACTATCAGCAAATAAAAAAAGGAATGGATTGA

Upstream 100 bases:

>100_bases
AAATAAAAGTGCGTTTAGTAGAAATTAAAAGGAACTCCTCTAGTTAAGAAAAAATCATAAGCATGTTATTTTATGGTTTT
TCCATCAATAGAAGAAAATA

Downstream 100 bases:

>100_bases
CAAGAGATTAACTAAAATTTGGAGAGACCCCATTGAATAAAAAAAGTTGGACAGAGCTGATTGCCTGGATCATTACCTTT
GAAGCAATTGGTTTTTTTTT

Product: deoxyribodipyrimidine photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTIALVWFRQDLRLTDNPAFIEACSHHEIVIPLYIYDDKCSVLGQAQAWWLHYSLIALGKSLNQQGLSLVLRKGSPQEII
LELVAQYGVESVYWNRCYEPQVIARDKKIKLALVEREIKVQSFNGNLLHEPWTIKNKNGDFFKVFTPYWKQCRQILNIPS
PFILTKRPRGIEIASDDIGKWKLLPAINWAARFSEYWIPGEEGAQKKLDEFIVHHLSGYKRDRDFPAKNATSRLSPHLHF
GEISPWVILRALELAKLEHTCDLASVEHFLSELGWREFSVYLLYHFPKLDCENFRKEFDAFPWQNDEQLLTCWQKGMTGY
PIIDAGMRELWATGYMHNRVRMIVASFLTKDLLIDWRSGAQWFLDTLVDADLANNSASWQWVAGCGADAAPYFRIFNPIL
QSQKFDPDGVYIRQWIPELAQLNSQSIHQPWTDADASSIFLGKKYPKPIIDHQEARTKALSYYQQIKKGMD

Sequences:

>Translated_471_residues
MTIALVWFRQDLRLTDNPAFIEACSHHEIVIPLYIYDDKCSVLGQAQAWWLHYSLIALGKSLNQQGLSLVLRKGSPQEII
LELVAQYGVESVYWNRCYEPQVIARDKKIKLALVEREIKVQSFNGNLLHEPWTIKNKNGDFFKVFTPYWKQCRQILNIPS
PFILTKRPRGIEIASDDIGKWKLLPAINWAARFSEYWIPGEEGAQKKLDEFIVHHLSGYKRDRDFPAKNATSRLSPHLHF
GEISPWVILRALELAKLEHTCDLASVEHFLSELGWREFSVYLLYHFPKLDCENFRKEFDAFPWQNDEQLLTCWQKGMTGY
PIIDAGMRELWATGYMHNRVRMIVASFLTKDLLIDWRSGAQWFLDTLVDADLANNSASWQWVAGCGADAAPYFRIFNPIL
QSQKFDPDGVYIRQWIPELAQLNSQSIHQPWTDADASSIFLGKKYPKPIIDHQEARTKALSYYQQIKKGMD
>Mature_470_residues
TIALVWFRQDLRLTDNPAFIEACSHHEIVIPLYIYDDKCSVLGQAQAWWLHYSLIALGKSLNQQGLSLVLRKGSPQEIIL
ELVAQYGVESVYWNRCYEPQVIARDKKIKLALVEREIKVQSFNGNLLHEPWTIKNKNGDFFKVFTPYWKQCRQILNIPSP
FILTKRPRGIEIASDDIGKWKLLPAINWAARFSEYWIPGEEGAQKKLDEFIVHHLSGYKRDRDFPAKNATSRLSPHLHFG
EISPWVILRALELAKLEHTCDLASVEHFLSELGWREFSVYLLYHFPKLDCENFRKEFDAFPWQNDEQLLTCWQKGMTGYP
IIDAGMRELWATGYMHNRVRMIVASFLTKDLLIDWRSGAQWFLDTLVDADLANNSASWQWVAGCGADAAPYFRIFNPILQ
SQKFDPDGVYIRQWIPELAQLNSQSIHQPWTDADASSIFLGKKYPKPIIDHQEARTKALSYYQQIKKGMD

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=493, Percent_Identity=28.8032454361055, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4758072, Length=489, Percent_Identity=26.9938650306748, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI188536103, Length=453, Percent_Identity=28.0353200883002, Blast_Score=149, Evalue=7e-36,
Organism=Escherichia coli, GI1786926, Length=486, Percent_Identity=38.8888888888889, Blast_Score=305, Evalue=4e-84,
Organism=Saccharomyces cerevisiae, GI6324962, Length=517, Percent_Identity=25.9187620889749, Blast_Score=176, Evalue=7e-45,
Organism=Drosophila melanogaster, GI17137248, Length=477, Percent_Identity=27.6729559748428, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24585455, Length=477, Percent_Identity=27.6729559748428, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24648152, Length=515, Percent_Identity=25.4368932038835, Blast_Score=152, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 54712; Mature: 54581

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIALVWFRQDLRLTDNPAFIEACSHHEIVIPLYIYDDKCSVLGQAQAWWLHYSLIALGK
CEEEEEEEECCCCCCCCCHHHHHCCCCCEEEEEEEECCCCCHHCCHHHHHHHHHHHHHHH
SLNQQGLSLVLRKGSPQEIILELVAQYGVESVYWNRCYEPQVIARDKKIKLALVEREIKV
HCCCCCHHEEECCCCHHHHHHHHHHHHCCHHHHHHCCCCCCEEECCCEEEEEEEEEEEEE
QSFNGNLLHEPWTIKNKNGDFFKVFTPYWKQCRQILNIPSPFILTKRPRGIEIASDDIGK
EECCCCEECCCEEEECCCCCEEEECCHHHHHHHHHHCCCCCEEEECCCCCCEEECCCCCC
WKLLPAINWAARFSEYWIPGEEGAQKKLDEFIVHHLSGYKRDRDFPAKNATSRLSPHLHF
EEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCC
GEISPWVILRALELAKLEHTCDLASVEHFLSELGWREFSVYLLYHFPKLDCENFRKEFDA
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHCC
FPWQNDEQLLTCWQKGMTGYPIIDAGMRELWATGYMHNRVRMIVASFLTKDLLIDWRSGA
CCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCH
QWFLDTLVDADLANNSASWQWVAGCGADAAPYFRIFNPILQSQKFDPDGVYIRQWIPELA
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
QLNSQSIHQPWTDADASSIFLGKKYPKPIIDHQEARTKALSYYQQIKKGMD
HCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIALVWFRQDLRLTDNPAFIEACSHHEIVIPLYIYDDKCSVLGQAQAWWLHYSLIALGK
EEEEEEEECCCCCCCCCHHHHHCCCCCEEEEEEEECCCCCHHCCHHHHHHHHHHHHHHH
SLNQQGLSLVLRKGSPQEIILELVAQYGVESVYWNRCYEPQVIARDKKIKLALVEREIKV
HCCCCCHHEEECCCCHHHHHHHHHHHHCCHHHHHHCCCCCCEEECCCEEEEEEEEEEEEE
QSFNGNLLHEPWTIKNKNGDFFKVFTPYWKQCRQILNIPSPFILTKRPRGIEIASDDIGK
EECCCCEECCCEEEECCCCCEEEECCHHHHHHHHHHCCCCCEEEECCCCCCEEECCCCCC
WKLLPAINWAARFSEYWIPGEEGAQKKLDEFIVHHLSGYKRDRDFPAKNATSRLSPHLHF
EEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCC
GEISPWVILRALELAKLEHTCDLASVEHFLSELGWREFSVYLLYHFPKLDCENFRKEFDA
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHCC
FPWQNDEQLLTCWQKGMTGYPIIDAGMRELWATGYMHNRVRMIVASFLTKDLLIDWRSGA
CCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCH
QWFLDTLVDADLANNSASWQWVAGCGADAAPYFRIFNPILQSQKFDPDGVYIRQWIPELA
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
QLNSQSIHQPWTDADASSIFLGKKYPKPIIDHQEARTKALSYYQQIKKGMD
HCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6325460; 8905232; 9278503; 2200511; 7604260 [H]