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The map label for this gene is nuoM-2 [H]

Identifier: 39998519

GI number: 39998519

Start: 3775526

End: 3777016

Strand: Reverse

Name: nuoM-2 [H]

Synonym: GSU3430

Alternate gene names: 39998519

Gene position: 3777016-3775526 (Counterclockwise)

Preceding gene: 39998520

Following gene: 39998518

Centisome position: 99.03

GC content: 64.86

Gene sequence:

>1491_bases
ATGACTTCCTACTCCATCCTCACCATCTTGATCCTCCTCCCCCTGGCGGGGTGCTTCTGCCTGGCGCCGGTCTGGAACCG
TCCGGAGTGGGCCCGCCCCCTCGCCCTGGGGATTGCCGTGGCGGAACTGGCCCTGGCCGGCTGGGTCCTCGTCGCCGCGC
CGGGAATGCCGCCGGCGCCGGGTGCCGCAGCAGGGTATTTTCTCTGGGAAGATGCCGCCTGGATCGAGCGGTTCGGCATC
CGGTACCTGCTGGGGATGGACGGGATCAGCCTCCTCATGGTGGCCCTCACCGCCTTCACCACGGTGGTGGCGATGCTCGT
TTCGTGGCGCGGCATCACCGAGCGGGCCACGCTCCACTACTTCCTGATCCTTCTCATGGAGAGCGGGATCATGGGAGTGT
TCCTTTCCCTTGACCTGGTCCTCTTCTACCTCTTCTGGGAAGTGATGCTGATCCCCATGTTCTTCTTGATCGGCATCTGG
GGCCACGGCCGACGCATCTACTCGGCAGTTAAGTTCTTCCTTTATACCCTGGTGGGCTCGCTCCTGATGCTGCTGGCCAT
CATCGGGGTCTACCTCATTCATGGCGATGCCACCGGCACCTTCACCTTTGCCCTGCCGCTCCTGGCCAAGTCACCCATTG
CCCATGCCGCGGCTCCGTGGCTTTTCGGGGCGTTTCTGCTGGCCTTCGCCATCAAGTTTCCGCTGTTTCCGGTCCACACC
TGGCTCCCGGACGCCCACACCGACGCCCCCACGGCCGGCAGCGTGATCCTGGCGGCGCTGCTCCTGAAGACCGGGGCCTA
CGGTCTGGTCCGCTTCGGCTACCCGCTCTTTCCCGAGGCGGCCAAAGGATTCACGCCGCTCCTCTACGTGCTGGCAATCA
TCGGCATCCTCTATGCCTCGTGGATCGCCTACGCCCAGGAGGACATGAAACGGATGGTGGCCTACTCCAGCGTTGGGCAC
ATGGGGTTCGTCGCCCTCGGGATCGCCTCCTGGGGGCCGGTGGCCCTGTCGGGCTCCATTCTCCAGATGGTGAACCACGG
CTTCACCACCGCCGCTCTCTTCGCCCTGGTGGGGATGCTGGACGAGCGCGCCCACACCCGGGAGGTGTCGGCGTTCGGCG
GACTCTGGGGAACCATGCCGGCCTTCTCCTTTTTCTTTCTCTTCTTCGCCATGGCTTCGGCGGGGCTGCCGGGGCTCAAC
AACTTCGTGGGAGAGTTCCTGATCCTGGTGGGGGTCTTCCGGATCACGCCAGCAGCCGGGGCAATCGCCTTCCTCGGTAT
CGTGCTGCCGCTTATCTACACCGTGCGACTCGTGCAGGAGGTTCTCTTCCAGACGGAACGGCGGCCACTGCGCCTGCCCG
ACCTGACCCTGCGTGAGGGGGCGGTGCTGGCCGTACTGGCCGTGATCGATCTCTACATCGGGGTCCATCCGAAACCGCTC
CTGGATATCCTCAAGGTGCCGGTGGCGCTGCTGACGGGGGTGGCACCGTGA

Upstream 100 bases:

>100_bases
GGCTGGGCCGCTGGAGTTGCGGACGGGTGTCGGTTTATCTCCTCAGCTTCGCGGCGGGGCTGGCGCTGGTTCTGGGGTGG
CTCGCATGGGGGAGGATCTG

Downstream 100 bases:

>100_bases
CCATCGCCGATCTCTGGACCATCATGCCGCTTCTGCTCCTCGCCTGCGGGAGCGTCCTCGTGCTTATCCTGGGTGCGATC
GTGCCTGGCCGGTACGGGAC

Product: NADH dehydrogenase I subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MTSYSILTILILLPLAGCFCLAPVWNRPEWARPLALGIAVAELALAGWVLVAAPGMPPAPGAAAGYFLWEDAAWIERFGI
RYLLGMDGISLLMVALTAFTTVVAMLVSWRGITERATLHYFLILLMESGIMGVFLSLDLVLFYLFWEVMLIPMFFLIGIW
GHGRRIYSAVKFFLYTLVGSLLMLLAIIGVYLIHGDATGTFTFALPLLAKSPIAHAAAPWLFGAFLLAFAIKFPLFPVHT
WLPDAHTDAPTAGSVILAALLLKTGAYGLVRFGYPLFPEAAKGFTPLLYVLAIIGILYASWIAYAQEDMKRMVAYSSVGH
MGFVALGIASWGPVALSGSILQMVNHGFTTAALFALVGMLDERAHTREVSAFGGLWGTMPAFSFFFLFFAMASAGLPGLN
NFVGEFLILVGVFRITPAAGAIAFLGIVLPLIYTVRLVQEVLFQTERRPLRLPDLTLREGAVLAVLAVIDLYIGVHPKPL
LDILKVPVALLTGVAP

Sequences:

>Translated_496_residues
MTSYSILTILILLPLAGCFCLAPVWNRPEWARPLALGIAVAELALAGWVLVAAPGMPPAPGAAAGYFLWEDAAWIERFGI
RYLLGMDGISLLMVALTAFTTVVAMLVSWRGITERATLHYFLILLMESGIMGVFLSLDLVLFYLFWEVMLIPMFFLIGIW
GHGRRIYSAVKFFLYTLVGSLLMLLAIIGVYLIHGDATGTFTFALPLLAKSPIAHAAAPWLFGAFLLAFAIKFPLFPVHT
WLPDAHTDAPTAGSVILAALLLKTGAYGLVRFGYPLFPEAAKGFTPLLYVLAIIGILYASWIAYAQEDMKRMVAYSSVGH
MGFVALGIASWGPVALSGSILQMVNHGFTTAALFALVGMLDERAHTREVSAFGGLWGTMPAFSFFFLFFAMASAGLPGLN
NFVGEFLILVGVFRITPAAGAIAFLGIVLPLIYTVRLVQEVLFQTERRPLRLPDLTLREGAVLAVLAVIDLYIGVHPKPL
LDILKVPVALLTGVAP
>Mature_495_residues
TSYSILTILILLPLAGCFCLAPVWNRPEWARPLALGIAVAELALAGWVLVAAPGMPPAPGAAAGYFLWEDAAWIERFGIR
YLLGMDGISLLMVALTAFTTVVAMLVSWRGITERATLHYFLILLMESGIMGVFLSLDLVLFYLFWEVMLIPMFFLIGIWG
HGRRIYSAVKFFLYTLVGSLLMLLAIIGVYLIHGDATGTFTFALPLLAKSPIAHAAAPWLFGAFLLAFAIKFPLFPVHTW
LPDAHTDAPTAGSVILAALLLKTGAYGLVRFGYPLFPEAAKGFTPLLYVLAIIGILYASWIAYAQEDMKRMVAYSSVGHM
GFVALGIASWGPVALSGSILQMVNHGFTTAALFALVGMLDERAHTREVSAFGGLWGTMPAFSFFFLFFAMASAGLPGLNN
FVGEFLILVGVFRITPAAGAIAFLGIVLPLIYTVRLVQEVLFQTERRPLRLPDLTLREGAVLAVLAVIDLYIGVHPKPLL
DILKVPVALLTGVAP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=302, Percent_Identity=34.4370860927152, Blast_Score=169, Evalue=7e-42,
Organism=Homo sapiens, GI251831117, Length=323, Percent_Identity=28.7925696594427, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788613, Length=495, Percent_Identity=41.2121212121212, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1788831, Length=363, Percent_Identity=29.4765840220386, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1788827, Length=296, Percent_Identity=27.3648648648649, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788829, Length=335, Percent_Identity=30.7462686567164, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788614, Length=469, Percent_Identity=27.7185501066098, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI145693160, Length=432, Percent_Identity=22.6851851851852, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI2367154, Length=318, Percent_Identity=25.1572327044025, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53772; Mature: 53641

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSYSILTILILLPLAGCFCLAPVWNRPEWARPLALGIAVAELALAGWVLVAAPGMPPAP
CCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCC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HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]