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The map label for this gene is nuoL-2 [H]

Identifier: 39998520

GI number: 39998520

Start: 3777016

End: 3778890

Strand: Reverse

Name: nuoL-2 [H]

Synonym: GSU3431

Alternate gene names: 39998520

Gene position: 3778890-3777016 (Counterclockwise)

Preceding gene: 39998521

Following gene: 39998519

Centisome position: 99.08

GC content: 66.99

Gene sequence:

>1875_bases
ATGAAGCTCTATCTCGCCCTCATACTCCTGCTGCCGCTGCTGGGCGGCACCCTCAACGCGCTGGTGGGGCGGAAGCTCCC
GCGCCGCCTGTGCGAGGCGCTGGCCTGTGCCGCGGTCTGGGGGGCCTTCGTCTGCGCGGCCCTGGCCTTCGCGGCCTATG
AAGCGCCGGAGACCGTGCTGCTGGCCACTTGGCTGGCGGACTTCGACTTCACCGCCCCCATCGCCCTCTACCTGGATCCC
CTCTCCCTAGTGATGACGGTGATGATCACCTTCGTCTGCGGCCTGATCCACCTCTACGCCGTGGGATATATGGCGGGGGA
GGGGAGCCCGGCCCGGTTCTTCGCGCTGCTGAATCTCTTCGTCTTTGCCATGCTGGTCCTGATCCTGGCGGAGAACCTGC
CGCTTCTCTACCTGGGGTGGGAAGGGGTCGGCTTCTGCTCCTACGCCCTCATCGGTTTCTGGTATTCCGACCCGAAAAAC
GCCACTGCCGGCCGCAAGGCCTTCATCACCACCCGAATCGGCGATACGGCCTTCGGCATCGGCATTGTCTGGTGCTTCCA
GCTTTTCCAGACCGAATCCATCAGCCAGATCAACCAGATGGGATTTCTGATGCCCGTGGGGGTCGTCACGGCGCTGGGGC
TTTTGTTTCTGGCCGGGGCCATGGGGAAGTCGGCCCAGGTACCCCTCATGGTCTGGCTCCCCGACGCCATGGCCGGCCCA
ACGCCGGTCTCGGCACTGATCCACGCCGCCACTATGGTCACCGCCGGGGTCTACCTCATGGCCCGGATGTCGCCCCTCTT
CAGCGCGGCCCCCGTGGTCATGGCCGCCGTGGCCATAACCGGTGCCGTCACCGCCTTCTACGGCGCCACCTGCGCCTTGG
TCCAGCGGGACTTCAAGCGGGTGCTGGCCTACTCCACCATCAGCCAGATCGGCTACATGATGCTCGGCGTCGGGGCCGGC
GCCGTCACTGCCGCCACCTTCCACCTGCTGGTGCATGCCTTCTTCAAGGCGCTTCTGTTCCTCGGGGCCGGCTGCGTCAT
CGCGGCCCTCCATCACGAGCAGGACCTCTTCAGGATGGGGGGCCTGCGCCGGAAGATGCCGGTCACCTTCTGGGCCTTCA
TGGCCGGTGCCGCCTGCCTGGCGGGACTTCCCCCCACCGGCGGCTTCTTCAGCAAGGACGCAATCCTGGCCGCGGTCTGG
GCCAAGGGGGGCGCGCTCTACGGCGGGCTCTTCGGTTTGGGGCTCATGGCGGCGCTCCTCACCTCCTTCTACACCTTCCG
AATGGTCTACCTCGTCTTCGGCGGCGAGGGGGCAACGGAGGTTCACCGTAATCCGCGGATCATGGACCTGATGCTCCTTC
CCCTGGCGATCCTGGGGCTCATCGGTGGGTTTATCCACCTCCCCGCGTTCCTGGCCGACGGCTGGCTCGGCCGCTTCCTG
GCCACGACCCTCACCGAAGGGGCTGCCCATCTTTCCCACGGGGAAGAGATGGCCGTGGAGGCAATCGCCGCCCTCGTGGC
CCTGACCGGACTCGCAATCGCCCACTACCGCTACGGCGGCATCCGCCGCGCGGCCCGGATCGAAGCAGCCGCAGCCGAGC
CCAGGGGGATCACCGCTTTCCTCCTGAACGGCTGGTACGCGGACCATCTCTACCGCGCCCTCTTCATCCGCCCCTACGAG
GCCATCGCCTCCTTCCTCTGGCGCCGGGTGGACGAAGGGGTGATCGACGTCTCTCTCGACCGCCTGGCCGACGGCATCGG
TACGGCCGGACAAGGGCTGGGCCGCTGGAGTTGCGGACGGGTGTCGGTTTATCTCCTCAGCTTCGCGGCGGGGCTGGCGC
TGGTTCTGGGGTGGCTCGCATGGGGGAGGATCTGA

Upstream 100 bases:

>100_bases
CCCTCACCTCGGCCGAGGTGTCCCTGGCCCTGGCCATGGTGGTCTACCTCCACCGGCGGAAGAAGACGGTTGATGCGGAT
GAGTTCAGGGAGCTGCAGGG

Downstream 100 bases:

>100_bases
TGACTTCCTACTCCATCCTCACCATCTTGATCCTCCTCCCCCTGGCGGGGTGCTTCTGCCTGGCGCCGGTCTGGAACCGT
CCGGAGTGGGCCCGCCCCCT

Product: NADH dehydrogenase I subunit L

Products: NA

Alternate protein names: NADH dehydrogenase I subunit L; NDH-1 subunit L; NUO12 [H]

Number of amino acids: Translated: 624; Mature: 624

Protein sequence:

>624_residues
MKLYLALILLLPLLGGTLNALVGRKLPRRLCEALACAAVWGAFVCAALAFAAYEAPETVLLATWLADFDFTAPIALYLDP
LSLVMTVMITFVCGLIHLYAVGYMAGEGSPARFFALLNLFVFAMLVLILAENLPLLYLGWEGVGFCSYALIGFWYSDPKN
ATAGRKAFITTRIGDTAFGIGIVWCFQLFQTESISQINQMGFLMPVGVVTALGLLFLAGAMGKSAQVPLMVWLPDAMAGP
TPVSALIHAATMVTAGVYLMARMSPLFSAAPVVMAAVAITGAVTAFYGATCALVQRDFKRVLAYSTISQIGYMMLGVGAG
AVTAATFHLLVHAFFKALLFLGAGCVIAALHHEQDLFRMGGLRRKMPVTFWAFMAGAACLAGLPPTGGFFSKDAILAAVW
AKGGALYGGLFGLGLMAALLTSFYTFRMVYLVFGGEGATEVHRNPRIMDLMLLPLAILGLIGGFIHLPAFLADGWLGRFL
ATTLTEGAAHLSHGEEMAVEAIAALVALTGLAIAHYRYGGIRRAARIEAAAAEPRGITAFLLNGWYADHLYRALFIRPYE
AIASFLWRRVDEGVIDVSLDRLADGIGTAGQGLGRWSCGRVSVYLLSFAAGLALVLGWLAWGRI

Sequences:

>Translated_624_residues
MKLYLALILLLPLLGGTLNALVGRKLPRRLCEALACAAVWGAFVCAALAFAAYEAPETVLLATWLADFDFTAPIALYLDP
LSLVMTVMITFVCGLIHLYAVGYMAGEGSPARFFALLNLFVFAMLVLILAENLPLLYLGWEGVGFCSYALIGFWYSDPKN
ATAGRKAFITTRIGDTAFGIGIVWCFQLFQTESISQINQMGFLMPVGVVTALGLLFLAGAMGKSAQVPLMVWLPDAMAGP
TPVSALIHAATMVTAGVYLMARMSPLFSAAPVVMAAVAITGAVTAFYGATCALVQRDFKRVLAYSTISQIGYMMLGVGAG
AVTAATFHLLVHAFFKALLFLGAGCVIAALHHEQDLFRMGGLRRKMPVTFWAFMAGAACLAGLPPTGGFFSKDAILAAVW
AKGGALYGGLFGLGLMAALLTSFYTFRMVYLVFGGEGATEVHRNPRIMDLMLLPLAILGLIGGFIHLPAFLADGWLGRFL
ATTLTEGAAHLSHGEEMAVEAIAALVALTGLAIAHYRYGGIRRAARIEAAAAEPRGITAFLLNGWYADHLYRALFIRPYE
AIASFLWRRVDEGVIDVSLDRLADGIGTAGQGLGRWSCGRVSVYLLSFAAGLALVLGWLAWGRI
>Mature_624_residues
MKLYLALILLLPLLGGTLNALVGRKLPRRLCEALACAAVWGAFVCAALAFAAYEAPETVLLATWLADFDFTAPIALYLDP
LSLVMTVMITFVCGLIHLYAVGYMAGEGSPARFFALLNLFVFAMLVLILAENLPLLYLGWEGVGFCSYALIGFWYSDPKN
ATAGRKAFITTRIGDTAFGIGIVWCFQLFQTESISQINQMGFLMPVGVVTALGLLFLAGAMGKSAQVPLMVWLPDAMAGP
TPVSALIHAATMVTAGVYLMARMSPLFSAAPVVMAAVAITGAVTAFYGATCALVQRDFKRVLAYSTISQIGYMMLGVGAG
AVTAATFHLLVHAFFKALLFLGAGCVIAALHHEQDLFRMGGLRRKMPVTFWAFMAGAACLAGLPPTGGFFSKDAILAAVW
AKGGALYGGLFGLGLMAALLTSFYTFRMVYLVFGGEGATEVHRNPRIMDLMLLPLAILGLIGGFIHLPAFLADGWLGRFL
ATTLTEGAAHLSHGEEMAVEAIAALVALTGLAIAHYRYGGIRRAARIEAAAAEPRGITAFLLNGWYADHLYRALFIRPYE
AIASFLWRRVDEGVIDVSLDRLADGIGTAGQGLGRWSCGRVSVYLLSFAAGLALVLGWLAWGRI

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=381, Percent_Identity=38.5826771653543, Blast_Score=243, Evalue=6e-64,
Organism=Escherichia coli, GI1788614, Length=613, Percent_Identity=42.9037520391517, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1788829, Length=415, Percent_Identity=33.2530120481928, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788827, Length=367, Percent_Identity=31.0626702997275, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788831, Length=366, Percent_Identity=29.5081967213115, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI2367154, Length=377, Percent_Identity=28.3819628647215, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788613, Length=425, Percent_Identity=28.7058823529412, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 66597; Mature: 66597

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYLALILLLPLLGGTLNALVGRKLPRRLCEALACAAVWGAFVCAALAFAAYEAPETVL
CHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LATWLADFDFTAPIALYLDPLSLVMTVMITFVCGLIHLYAVGYMAGEGSPARFFALLNLF
HHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VFAMLVLILAENLPLLYLGWEGVGFCSYALIGFWYSDPKNATAGRKAFITTRIGDTAFGI
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHH
GIVWCFQLFQTESISQINQMGFLMPVGVVTALGLLFLAGAMGKSAQVPLMVWLPDAMAGP
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCC
TPVSALIHAATMVTAGVYLMARMSPLFSAAPVVMAAVAITGAVTAFYGATCALVQRDFKR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLAYSTISQIGYMMLGVGAGAVTAATFHLLVHAFFKALLFLGAGCVIAALHHEQDLFRMG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
GLRRKMPVTFWAFMAGAACLAGLPPTGGFFSKDAILAAVWAKGGALYGGLFGLGLMAALL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TSFYTFRMVYLVFGGEGATEVHRNPRIMDLMLLPLAILGLIGGFIHLPAFLADGWLGRFL
HHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATTLTEGAAHLSHGEEMAVEAIAALVALTGLAIAHYRYGGIRRAARIEAAAAEPRGITAF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEH
LLNGWYADHLYRALFIRPYEAIASFLWRRVDEGVIDVSLDRLADGIGTAGQGLGRWSCGR
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHEEEHHHHHHCCCCCCCCCCCCCCCH
VSVYLLSFAAGLALVLGWLAWGRI
HHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLYLALILLLPLLGGTLNALVGRKLPRRLCEALACAAVWGAFVCAALAFAAYEAPETVL
CHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LATWLADFDFTAPIALYLDPLSLVMTVMITFVCGLIHLYAVGYMAGEGSPARFFALLNLF
HHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VFAMLVLILAENLPLLYLGWEGVGFCSYALIGFWYSDPKNATAGRKAFITTRIGDTAFGI
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHH
GIVWCFQLFQTESISQINQMGFLMPVGVVTALGLLFLAGAMGKSAQVPLMVWLPDAMAGP
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCC
TPVSALIHAATMVTAGVYLMARMSPLFSAAPVVMAAVAITGAVTAFYGATCALVQRDFKR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLAYSTISQIGYMMLGVGAGAVTAATFHLLVHAFFKALLFLGAGCVIAALHHEQDLFRMG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
GLRRKMPVTFWAFMAGAACLAGLPPTGGFFSKDAILAAVWAKGGALYGGLFGLGLMAALL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TSFYTFRMVYLVFGGEGATEVHRNPRIMDLMLLPLAILGLIGGFIHLPAFLADGWLGRFL
HHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATTLTEGAAHLSHGEEMAVEAIAALVALTGLAIAHYRYGGIRRAARIEAAAAEPRGITAF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEH
LLNGWYADHLYRALFIRPYEAIASFLWRRVDEGVIDVSLDRLADGIGTAGQGLGRWSCGR
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHEEEHHHHHHCCCCCCCCCCCCCCCH
VSVYLLSFAAGLALVLGWLAWGRI
HHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7690854; 9205837; 9278503 [H]