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The map label for this gene is nuoN-2 [H]

Identifier: 39998518

GI number: 39998518

Start: 3774129

End: 3775529

Strand: Reverse

Name: nuoN-2 [H]

Synonym: GSU3429

Alternate gene names: 39998518

Gene position: 3775529-3774129 (Counterclockwise)

Preceding gene: 39998519

Following gene: 39998517

Centisome position: 98.99

GC content: 68.74

Gene sequence:

>1401_bases
GTGACCATCGCCGATCTCTGGACCATCATGCCGCTTCTGCTCCTCGCCTGCGGGAGCGTCCTCGTGCTTATCCTGGGTGC
GATCGTGCCTGGCCGGTACGGGACTGCCATCGGAGTGGCGGTCTGCGCCGGTGCGGCCCTCTGGGCTCTGCAGACCCCCC
CCCGGCCCCTGGCGCCGACCCTGGGGGTGGCGTTCACGCCGTTCGCCCGGTCGTTTTTGGTGCTTTTCGCGGTGACCGCG
CTCCTGTCCCTGCTCCTTGCCCATGACCACGCCGACCGGCAGGGATTGGAGGGAGAGGAGTATCCGGCCACGATCCTGTT
CGGCGCATTCGGCATGGGGGTGGTGGCGAGCGCGGCCAACTTCCTGACCCTCTTTCTGGGGCTGGAGGCCCTGACCTTCG
CATTCTACATCCTGGTGGCCTATGATCGCAACCGGCCGGCATCGGGGGAAGCGGGACTCAAGTATCTCCTCATGGGTGCC
GTGTCCGCGGCATTCGTGGCCTTCGGCATCGCGCTCCTCTATGGTGCCACCGGCTCCCTGGAGATCGGGCGGGCCGTTGC
CGCCTCGGCAGCGGGCGGCGGGATCGCCCTGGCGGGATGGGGGCTCCTCCTGGCGGGGCTCGCCTTCAAGGTGTCGCTGG
CCCCGGCCCACCTCTGGACCCCGGATGTCTACCAGGGGGGGCCGACGCCGGTGACCGCCTTCCTGGCCGCCGGTTCCAAG
GGAGCGGCCATCGCCCTGTTCCTGCTGATCCTGCCCCCCCTGGGGGAAATCGGTCCCCTGCGGGCGCCCCTCTGGGGGCT
GGCGCTCCTCTCCATGGCCGTGGGGAACCTGGCGGCGCTGCTCCAGCCCAATGTGAAGCGGATGCTCGCCTACTCCTCCG
TGGCCCAGATGGGGTACGTGGCCCTGGCGCTCCTCTCTGGTGGACGGGGCTACGAGGCCGCGGCCTTCTATGCCGTTGCC
TATGGGGTCATGAGCCTGGCGGCCTTCGGTGCCCTTGCCTCCCTGGAACAGGAAAGACCGCTGGAGCAGGTGGATGAGCT
CCGGGGGATCGGCTACCGGCGGCCGTTCCAGGGGGTGGTACTGGCGGTGGCCATGTTTGCCCTGGCGGGAATTCCCCCCA
CCGTGGGTTTTGCGGGGAAATTCGCCATCTTTTTCGCGGCCCTCAGGGCAGGAGAGACGCCCCTGGCGATCATCGGCATC
CTCACGGCAGCGGCGTCGGCCTACTACTACCTGCGGGTGGTGGTGAACCTCTACATGAAACCGGCTGAGGAGGCTGCCCC
GGGTCCGCTCCCCACCCCGGCGGAGGCGGTATGCCTCGGCGCCGTGACCGTCGCCATCCTGGCTCTCGGCGTCTGGCCCG
GTCCTTTGTTCGACTTGGTTGAGGCGATCCTCAAGGCCTGA

Upstream 100 bases:

>100_bases
TGGCCGTACTGGCCGTGATCGATCTCTACATCGGGGTCCATCCGAAACCGCTCCTGGATATCCTCAAGGTGCCGGTGGCG
CTGCTGACGGGGGTGGCACC

Downstream 100 bases:

>100_bases
GCCACGTGTTCGGGAGGAACCAGATGAGACGCATGCTCGCCACCTGCCTGTTTGCGACGCTGTTCGGTACGCTCCCGGCC
TTTGGTGCCGAAGGCGACGC

Product: NADH dehydrogenase I, subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MTIADLWTIMPLLLLACGSVLVLILGAIVPGRYGTAIGVAVCAGAALWALQTPPRPLAPTLGVAFTPFARSFLVLFAVTA
LLSLLLAHDHADRQGLEGEEYPATILFGAFGMGVVASAANFLTLFLGLEALTFAFYILVAYDRNRPASGEAGLKYLLMGA
VSAAFVAFGIALLYGATGSLEIGRAVAASAAGGGIALAGWGLLLAGLAFKVSLAPAHLWTPDVYQGGPTPVTAFLAAGSK
GAAIALFLLILPPLGEIGPLRAPLWGLALLSMAVGNLAALLQPNVKRMLAYSSVAQMGYVALALLSGGRGYEAAAFYAVA
YGVMSLAAFGALASLEQERPLEQVDELRGIGYRRPFQGVVLAVAMFALAGIPPTVGFAGKFAIFFAALRAGETPLAIIGI
LTAAASAYYYLRVVVNLYMKPAEEAAPGPLPTPAEAVCLGAVTVAILALGVWPGPLFDLVEAILKA

Sequences:

>Translated_466_residues
MTIADLWTIMPLLLLACGSVLVLILGAIVPGRYGTAIGVAVCAGAALWALQTPPRPLAPTLGVAFTPFARSFLVLFAVTA
LLSLLLAHDHADRQGLEGEEYPATILFGAFGMGVVASAANFLTLFLGLEALTFAFYILVAYDRNRPASGEAGLKYLLMGA
VSAAFVAFGIALLYGATGSLEIGRAVAASAAGGGIALAGWGLLLAGLAFKVSLAPAHLWTPDVYQGGPTPVTAFLAAGSK
GAAIALFLLILPPLGEIGPLRAPLWGLALLSMAVGNLAALLQPNVKRMLAYSSVAQMGYVALALLSGGRGYEAAAFYAVA
YGVMSLAAFGALASLEQERPLEQVDELRGIGYRRPFQGVVLAVAMFALAGIPPTVGFAGKFAIFFAALRAGETPLAIIGI
LTAAASAYYYLRVVVNLYMKPAEEAAPGPLPTPAEAVCLGAVTVAILALGVWPGPLFDLVEAILKA
>Mature_465_residues
TIADLWTIMPLLLLACGSVLVLILGAIVPGRYGTAIGVAVCAGAALWALQTPPRPLAPTLGVAFTPFARSFLVLFAVTAL
LSLLLAHDHADRQGLEGEEYPATILFGAFGMGVVASAANFLTLFLGLEALTFAFYILVAYDRNRPASGEAGLKYLLMGAV
SAAFVAFGIALLYGATGSLEIGRAVAASAAGGGIALAGWGLLLAGLAFKVSLAPAHLWTPDVYQGGPTPVTAFLAAGSKG
AAIALFLLILPPLGEIGPLRAPLWGLALLSMAVGNLAALLQPNVKRMLAYSSVAQMGYVALALLSGGRGYEAAAFYAVAY
GVMSLAAFGALASLEQERPLEQVDELRGIGYRRPFQGVVLAVAMFALAGIPPTVGFAGKFAIFFAALRAGETPLAIIGIL
TAAASAYYYLRVVVNLYMKPAEEAAPGPLPTPAEAVCLGAVTVAILALGVWPGPLFDLVEAILKA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Escherichia coli, GI145693160, Length=481, Percent_Identity=33.6798336798337, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1788614, Length=338, Percent_Identity=28.698224852071, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1788831, Length=378, Percent_Identity=28.042328042328, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788829, Length=336, Percent_Identity=25, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788613, Length=361, Percent_Identity=25.207756232687, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 47875; Mature: 47744

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIADLWTIMPLLLLACGSVLVLILGAIVPGRYGTAIGVAVCAGAALWALQTPPRPLAPT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
LGVAFTPFARSFLVLFAVTALLSLLLAHDHADRQGLEGEEYPATILFGAFGMGVVASAAN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
FLTLFLGLEALTFAFYILVAYDRNRPASGEAGLKYLLMGAVSAAFVAFGIALLYGATGSL
HHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EIGRAVAASAAGGGIALAGWGLLLAGLAFKVSLAPAHLWTPDVYQGGPTPVTAFLAAGSK
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHCCCC
GAAIALFLLILPPLGEIGPLRAPLWGLALLSMAVGNLAALLQPNVKRMLAYSSVAQMGYV
CHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ALALLSGGRGYEAAAFYAVAYGVMSLAAFGALASLEQERPLEQVDELRGIGYRRPFQGVV
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHH
LAVAMFALAGIPPTVGFAGKFAIFFAALRAGETPLAIIGILTAAASAYYYLRVVVNLYMK
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PAEEAAPGPLPTPAEAVCLGAVTVAILALGVWPGPLFDLVEAILKA
CHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TIADLWTIMPLLLLACGSVLVLILGAIVPGRYGTAIGVAVCAGAALWALQTPPRPLAPT
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
LGVAFTPFARSFLVLFAVTALLSLLLAHDHADRQGLEGEEYPATILFGAFGMGVVASAAN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
FLTLFLGLEALTFAFYILVAYDRNRPASGEAGLKYLLMGAVSAAFVAFGIALLYGATGSL
HHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EIGRAVAASAAGGGIALAGWGLLLAGLAFKVSLAPAHLWTPDVYQGGPTPVTAFLAAGSK
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHCCCC
GAAIALFLLILPPLGEIGPLRAPLWGLALLSMAVGNLAALLQPNVKRMLAYSSVAQMGYV
CHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ALALLSGGRGYEAAAFYAVAYGVMSLAAFGALASLEQERPLEQVDELRGIGYRRPFQGVV
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHH
LAVAMFALAGIPPTVGFAGKFAIFFAALRAGETPLAIIGILTAAASAYYYLRVVVNLYMK
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PAEEAAPGPLPTPAEAVCLGAVTVAILALGVWPGPLFDLVEAILKA
CHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA