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The map label for this gene is 39998506

Identifier: 39998506

GI number: 39998506

Start: 3759521

End: 3761050

Strand: Direct

Name: 39998506

Synonym: GSU3417

Alternate gene names: NA

Gene position: 3759521-3761050 (Clockwise)

Preceding gene: 39998505

Following gene: 39998509

Centisome position: 98.57

GC content: 66.86

Gene sequence:

>1530_bases
ATGAACAGAAGGGACTTCCTTAAAACCGCCGGACTGACCGGCATCGCCCTGGGGCTCCCCGGCTGCGCCCGGAGCCTCCC
CTTCGGCCGGGACGTCTTTCCCGACTTCGGTGACGATGCCCGCCCCTACCTGGGGCTTGCCACGTCGCTGCGGGAGGAGC
ACGACTACGAGGCCCGGGTGGAAGGAACCATCCCCGCGGGGCTGCGCGGCACCCTCTACCGCAACGGCCCGGCCCTCTTC
GATCGGGGTGGGATGCGCAAGCGGACCCTCCTGGACGGGGACGGCATGGTGCAGGCGTTCCGTTTCGGCGACCGTGGGAT
CAGATATGCGAACCGCTTCGTGCGGACCCGGAAATTCGTGGAAGAAGAGGCTGCCGGCCGCTTCCTCCATCCCACCTGGA
GCACCCAGGCCCCCGGCGGAATCTGGACCAACGTCTGGCCCACGGAGCGGCTCCTGAGCCAGGCGGGGATTACGGTCTTC
CCGTGGCGGGGACGGCTTTACGCCTTCGACGAATCATCTTTCCCCTACGAACTGGACCCGGATACCCTGGCAACCGTGGG
CGAAACCACCTTTGGCCTGCCGCGCGACCTCACCACTTACTCGGCCCATGGCAAGTTCGACCCGGTCACGGGAGAATGGC
TCCACTTCGGCATCCGCTACGGCCCCCGAACCTTTGTCCACCTGACCACCTTCAATGCGGACGGCACCCTCCGCCGCCAC
CGGGCCCTGGAGCTTCCCCGCGCGATCTACATGCACGACTGGTTCGTAACCGAGCGCCACGTGGTGTTCCACCTCCACCC
GGTGGAAATCGCCTACTGGCCCTTCCTGCTCGGCATCCGGAGCATGGCCGAATCCCTCCGCTGGCGCCCCGAACGGGGAA
CGATCCTCATGGTGGCGGAGCGGGACGGCGAGGCGCCGCCACGCCTGGTGGAAACCGAGGCATGCTATCTCTGGCACACC
TTCAACGCGTGGGAAGAACGAGGGAAAATCACTGCCGACTTCGTGGGGTACCGCAACCCGGACCATTTCATCAGCGACGA
TCCGGTCATAACCGCGGTCATGCTGGGACGGCGGGGAACCTATTCCTATCCCGGCGAGGTCATGCGCTACCGGATCGATC
CGGCCAGGGGAACGGCCGCCCGCGAGGTGCTCCATCAGGGGAGCTGCGAGTGGCCCCGCATCGACGAGCGGCTCCGTTGC
CGCCCACACCGCACGGGGTACATGCTCCGCTGCCTTCCCGGCGAATTCTTTTGGTCAATCGTCATGGGGCTCGACCCGGT
TACCGGCCGCACCGACGAGTACAGCTTCGGCCGGGGCGTCTACTGCACCGAGCCGGTCTTCGCGCCGCGGCCCGACACCC
TCGCCGGCGGCCCCGGCTGGCTCCTGGTCGAACTCTATGACAGCCGCACCCGCACCAGTTCACTGGCCATCCTCGACGCC
GACAGGATCGCCGATGGCCCCCTGGCCCTCGTTCGACTCACCCACCACGTCCCCTTCAGTTACCACGGCTGGTGGCAGCC
TGCTTCTTAG

Upstream 100 bases:

>100_bases
CGCTATCACCACGTCTCCAACGCCGGCACCGCCGAACCCAACGAGCCTCTGAATTCGAGCAAGTTTCTGCTGGGGGTATC
GGTCTACCGGTAGGTACGCC

Downstream 100 bases:

>100_bases
CACCTGCTGACGGTTCAATGGGCAGGAAAGAACTGCAGCACCCGCTCCAGCCCACTCCCTACGGCACGTGCGGGGGGAGC
GGGAGTGGCATCGGGGAACT

Product: dioxygenase

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MNRRDFLKTAGLTGIALGLPGCARSLPFGRDVFPDFGDDARPYLGLATSLREEHDYEARVEGTIPAGLRGTLYRNGPALF
DRGGMRKRTLLDGDGMVQAFRFGDRGIRYANRFVRTRKFVEEEAAGRFLHPTWSTQAPGGIWTNVWPTERLLSQAGITVF
PWRGRLYAFDESSFPYELDPDTLATVGETTFGLPRDLTTYSAHGKFDPVTGEWLHFGIRYGPRTFVHLTTFNADGTLRRH
RALELPRAIYMHDWFVTERHVVFHLHPVEIAYWPFLLGIRSMAESLRWRPERGTILMVAERDGEAPPRLVETEACYLWHT
FNAWEERGKITADFVGYRNPDHFISDDPVITAVMLGRRGTYSYPGEVMRYRIDPARGTAAREVLHQGSCEWPRIDERLRC
RPHRTGYMLRCLPGEFFWSIVMGLDPVTGRTDEYSFGRGVYCTEPVFAPRPDTLAGGPGWLLVELYDSRTRTSSLAILDA
DRIADGPLALVRLTHHVPFSYHGWWQPAS

Sequences:

>Translated_509_residues
MNRRDFLKTAGLTGIALGLPGCARSLPFGRDVFPDFGDDARPYLGLATSLREEHDYEARVEGTIPAGLRGTLYRNGPALF
DRGGMRKRTLLDGDGMVQAFRFGDRGIRYANRFVRTRKFVEEEAAGRFLHPTWSTQAPGGIWTNVWPTERLLSQAGITVF
PWRGRLYAFDESSFPYELDPDTLATVGETTFGLPRDLTTYSAHGKFDPVTGEWLHFGIRYGPRTFVHLTTFNADGTLRRH
RALELPRAIYMHDWFVTERHVVFHLHPVEIAYWPFLLGIRSMAESLRWRPERGTILMVAERDGEAPPRLVETEACYLWHT
FNAWEERGKITADFVGYRNPDHFISDDPVITAVMLGRRGTYSYPGEVMRYRIDPARGTAAREVLHQGSCEWPRIDERLRC
RPHRTGYMLRCLPGEFFWSIVMGLDPVTGRTDEYSFGRGVYCTEPVFAPRPDTLAGGPGWLLVELYDSRTRTSSLAILDA
DRIADGPLALVRLTHHVPFSYHGWWQPAS
>Mature_509_residues
MNRRDFLKTAGLTGIALGLPGCARSLPFGRDVFPDFGDDARPYLGLATSLREEHDYEARVEGTIPAGLRGTLYRNGPALF
DRGGMRKRTLLDGDGMVQAFRFGDRGIRYANRFVRTRKFVEEEAAGRFLHPTWSTQAPGGIWTNVWPTERLLSQAGITVF
PWRGRLYAFDESSFPYELDPDTLATVGETTFGLPRDLTTYSAHGKFDPVTGEWLHFGIRYGPRTFVHLTTFNADGTLRRH
RALELPRAIYMHDWFVTERHVVFHLHPVEIAYWPFLLGIRSMAESLRWRPERGTILMVAERDGEAPPRLVETEACYLWHT
FNAWEERGKITADFVGYRNPDHFISDDPVITAVMLGRRGTYSYPGEVMRYRIDPARGTAAREVLHQGSCEWPRIDERLRC
RPHRTGYMLRCLPGEFFWSIVMGLDPVTGRTDEYSFGRGVYCTEPVFAPRPDTLAGGPGWLLVELYDSRTRTSSLAILDA
DRIADGPLALVRLTHHVPFSYHGWWQPAS

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

Organism=Homo sapiens, GI82617628, Length=521, Percent_Identity=20.7293666026871, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI82617624, Length=521, Percent_Identity=20.7293666026871, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI74027270, Length=520, Percent_Identity=21.7307692307692, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI4506591, Length=514, Percent_Identity=22.3735408560311, Blast_Score=80, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71998242, Length=518, Percent_Identity=21.6216216216216, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI32564756, Length=527, Percent_Identity=20.6831119544592, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24646669, Length=307, Percent_Identity=24.4299674267101, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 58051; Mature: 58051

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRDFLKTAGLTGIALGLPGCARSLPFGRDVFPDFGDDARPYLGLATSLREEHDYEARV
CCHHHHHHHCCCCEEEECCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
EGTIPAGLRGTLYRNGPALFDRGGMRKRTLLDGDGMVQAFRFGDRGIRYANRFVRTRKFV
ECCCCCCCCCEEEECCCCEECCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHH
EEEAAGRFLHPTWSTQAPGGIWTNVWPTERLLSQAGITVFPWRGRLYAFDESSFPYELDP
HHHHCCCEECCCCCCCCCCCEECCCCCHHHHHHHCCCEEEECCCEEEEECCCCCCEECCC
DTLATVGETTFGLPRDLTTYSAHGKFDPVTGEWLHFGIRYGPRTFVHLTTFNADGTLRRH
CHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEEEECCEEEEEEEEECCCCCHHHH
RALELPRAIYMHDWFVTERHVVFHLHPVEIAYWPFLLGIRSMAESLRWRPERGTILMVAE
HHHHCCCEEEEEEEEEECCEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
RDGEAPPRLVETEACYLWHTFNAWEERGKITADFVGYRNPDHFISDDPVITAVMLGRRGT
CCCCCCCCEEECCEEEEEEECHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEEECCCCC
YSYPGEVMRYRIDPARGTAAREVLHQGSCEWPRIDERLRCRPHRTGYMLRCLPGEFFWSI
CCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCHHHCCCCCCCCEEEEECCHHHHHHH
VMGLDPVTGRTDEYSFGRGVYCTEPVFAPRPDTLAGGPGWLLVELYDSRTRTSSLAILDA
HHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEH
DRIADGPLALVRLTHHVPFSYHGWWQPAS
HHCCCCCEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MNRRDFLKTAGLTGIALGLPGCARSLPFGRDVFPDFGDDARPYLGLATSLREEHDYEARV
CCHHHHHHHCCCCEEEECCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
EGTIPAGLRGTLYRNGPALFDRGGMRKRTLLDGDGMVQAFRFGDRGIRYANRFVRTRKFV
ECCCCCCCCCEEEECCCCEECCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHH
EEEAAGRFLHPTWSTQAPGGIWTNVWPTERLLSQAGITVFPWRGRLYAFDESSFPYELDP
HHHHCCCEECCCCCCCCCCCEECCCCCHHHHHHHCCCEEEECCCEEEEECCCCCCEECCC
DTLATVGETTFGLPRDLTTYSAHGKFDPVTGEWLHFGIRYGPRTFVHLTTFNADGTLRRH
CHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEEEECCEEEEEEEEECCCCCHHHH
RALELPRAIYMHDWFVTERHVVFHLHPVEIAYWPFLLGIRSMAESLRWRPERGTILMVAE
HHHHCCCEEEEEEEEEECCEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
RDGEAPPRLVETEACYLWHTFNAWEERGKITADFVGYRNPDHFISDDPVITAVMLGRRGT
CCCCCCCCEEECCEEEEEEECHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEEECCCCC
YSYPGEVMRYRIDPARGTAAREVLHQGSCEWPRIDERLRCRPHRTGYMLRCLPGEFFWSI
CCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCHHHCCCCCCCCEEEEECCHHHHHHH
VMGLDPVTGRTDEYSFGRGVYCTEPVFAPRPDTLAGGPGWLLVELYDSRTRTSSLAILDA
HHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEH
DRIADGPLALVRLTHHVPFSYHGWWQPAS
HHCCCCCEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]