Click here to switch to the map view.

The map label for this gene is ispF

Identifier: 39998456

GI number: 39998456

Start: 3700792

End: 3701265

Strand: Reverse

Name: ispF

Synonym: GSU3367

Alternate gene names: 39998456

Gene position: 3701265-3700792 (Counterclockwise)

Preceding gene: 39998457

Following gene: 39998455

Centisome position: 97.04

GC content: 63.71

Gene sequence:

>474_bases
ATGAGGATCGGCCACGGCTATGACGTCCACCGCCTGGTGGCGGGACGGAAACTGATCGTCGGCGGCGTCGACATTCCCCA
CGAACTGGGCCTGCTGGGGCATTCGGATGCCGATGTGCTGCTCCACGCCATCTCCGACGCCATTCTCGGTGCCCTGGCCC
TGGGAGATATCGGCAAGCATTTTCCCGACACCGATCCCCGGTACAAGGGCGCCGACAGCCGGGCACTGCTCCGCCATGTC
ATGGAGCTGGCAACCCGGAAGGGATTCCACCTCGGCAACTTGGATGCCACGATCGTGGCCCAGCGTCCCAAGATGGCCCC
CCATATCCCCCTCATGCGAGAACATATTGCCGCTGACCTGATGGCGGACCCCGATCGGGTGAACGTCAAGGCGACCACCA
CCGAAGAACTTGGGTTCGCGGGGCGTGGCGAGGGGATCGCCGCCTATGCGGTGGTGCTCATGGAAGAGAAATAG

Upstream 100 bases:

>100_bases
ACGACGCCCGAGGACATGGTCCTGGCCGAGGCGTTTCTGAAGGAGCTCGCGGCGTGAGCGGGCGACCGGCTCCGCGCGTT
GCTAAAGAGAGGTGCATACC

Downstream 100 bases:

>100_bases
CAGCCCAAAGAATTGCGCGAGGACAGCCTAATGTCGACCACCGAACCAGCCCACGTCACCAACTTCCTGCGAACCATCGT
CGAGGATGATCTCAAAAGCG

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MRIGHGYDVHRLVAGRKLIVGGVDIPHELGLLGHSDADVLLHAISDAILGALALGDIGKHFPDTDPRYKGADSRALLRHV
MELATRKGFHLGNLDATIVAQRPKMAPHIPLMREHIAADLMADPDRVNVKATTTEELGFAGRGEGIAAYAVVLMEEK

Sequences:

>Translated_157_residues
MRIGHGYDVHRLVAGRKLIVGGVDIPHELGLLGHSDADVLLHAISDAILGALALGDIGKHFPDTDPRYKGADSRALLRHV
MELATRKGFHLGNLDATIVAQRPKMAPHIPLMREHIAADLMADPDRVNVKATTTEELGFAGRGEGIAAYAVVLMEEK
>Mature_157_residues
MRIGHGYDVHRLVAGRKLIVGGVDIPHELGLLGHSDADVLLHAISDAILGALALGDIGKHFPDTDPRYKGADSRALLRHV
MELATRKGFHLGNLDATIVAQRPKMAPHIPLMREHIAADLMADPDRVNVKATTTEELGFAGRGEGIAAYAVVLMEEK

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=64.9350649350649, Blast_Score=186, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_GEOSL (Q747A0)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_954407.1
- ProteinModelPortal:   Q747A0
- SMR:   Q747A0
- GeneID:   2688198
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU3367
- NMPDR:   fig|243231.1.peg.3345
- TIGR:   GSU3367
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   GSUL243231:GSU_3367-MONOMER
- BRENDA:   4.6.1.12
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16920; Mature: 16920

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS01350 ISPF; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGHGYDVHRLVAGRKLIVGGVDIPHELGLLGHSDADVLLHAISDAILGALALGDIGKH
CCCCCCCCHHHHHCCCEEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
FPDTDPRYKGADSRALLRHVMELATRKGFHLGNLDATIVAQRPKMAPHIPLMREHIAADL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCCHHHHHHHHHHH
MADPDRVNVKATTTEELGFAGRGEGIAAYAVVLMEEK
HCCCCCEEEEEECHHHCCCCCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MRIGHGYDVHRLVAGRKLIVGGVDIPHELGLLGHSDADVLLHAISDAILGALALGDIGKH
CCCCCCCCHHHHHCCCEEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
FPDTDPRYKGADSRALLRHVMELATRKGFHLGNLDATIVAQRPKMAPHIPLMREHIAADL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCCHHHHHHHHHHH
MADPDRVNVKATTTEELGFAGRGEGIAAYAVVLMEEK
HCCCCCEEEEEECHHHCCCCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA