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The map label for this gene is ispD

Identifier: 39998457

GI number: 39998457

Start: 3701309

End: 3702007

Strand: Reverse

Name: ispD

Synonym: GSU3368

Alternate gene names: 39998457

Gene position: 3702007-3701309 (Counterclockwise)

Preceding gene: 39998458

Following gene: 39998456

Centisome position: 97.06

GC content: 64.23

Gene sequence:

>699_bases
ATGGCCGTTTTCGCCCTGGTCCCGGCCGCCGGCATGGGTAAGCGGATGGGGGCCTCCATCAACAAACAGTACCTCATCCT
GGCGGGGAGACCGATCCTCGCCCATACCCTGAGTGTTTTCGAGGGGGCGTCCTTTGTTGACGGCATATTTGTCATCACTC
CCGAGGACGAGATTCCCTTCTGCCGTGACCACGTGGTTGAGCGCTACGGCTTCACCAAGGTTCGCGGCATCGTTGCCGGC
GGTGCGGAGCGCCAACATTCGGTCCTGAACGGGTTGCGGGCCATGGAGGGAACTGTGGCGGACGACGACGTGATCCTTAT
CCATGATGGGGTCCGCCCCTTTGTCAGTACGGACGTGCTGGCCCGGGCAACCGCGGTGGCCCGGGAGGACGACGGAGCCC
TTGTTGCCGTGCCGGCCAAAGACACGGTGAAGACGGTTGAAGACGGCATCATCACCGGTACCCCTCCCCGGGAGACCCTC
TGGCTCGCCCAGACGCCCCAGGCGTTTCGCTACGCCGTCATCCGCGCGGCTCACGAAATTGCCGACGCGGAACGCTTTCT
CGGCACCGACGATGCCATGCTCGTGGAGCGCCTGGGCCGCAGCGTGCGGATCGTCGTGGGAGATTACCGCAACATCAAGA
TCACGACGCCCGAGGACATGGTCCTGGCCGAGGCGTTTCTGAAGGAGCTCGCGGCGTGA

Upstream 100 bases:

>100_bases
TTCGGTATAATCAGCTCATTCCGGCCCGACCGGCGCCGTTTTCCGCGCAGGCCGGGCCGTATTTGTGGCGACGTTCAGCA
CGGCGCCAAGAGGAGAAACC

Downstream 100 bases:

>100_bases
GCGGGCGACCGGCTCCGCGCGTTGCTAAAGAGAGGTGCATACCATGAGGATCGGCCACGGCTATGACGTCCACCGCCTGG
TGGCGGGACGGAAACTGATC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MAVFALVPAAGMGKRMGASINKQYLILAGRPILAHTLSVFEGASFVDGIFVITPEDEIPFCRDHVVERYGFTKVRGIVAG
GAERQHSVLNGLRAMEGTVADDDVILIHDGVRPFVSTDVLARATAVAREDDGALVAVPAKDTVKTVEDGIITGTPPRETL
WLAQTPQAFRYAVIRAAHEIADAERFLGTDDAMLVERLGRSVRIVVGDYRNIKITTPEDMVLAEAFLKELAA

Sequences:

>Translated_232_residues
MAVFALVPAAGMGKRMGASINKQYLILAGRPILAHTLSVFEGASFVDGIFVITPEDEIPFCRDHVVERYGFTKVRGIVAG
GAERQHSVLNGLRAMEGTVADDDVILIHDGVRPFVSTDVLARATAVAREDDGALVAVPAKDTVKTVEDGIITGTPPRETL
WLAQTPQAFRYAVIRAAHEIADAERFLGTDDAMLVERLGRSVRIVVGDYRNIKITTPEDMVLAEAFLKELAA
>Mature_231_residues
AVFALVPAAGMGKRMGASINKQYLILAGRPILAHTLSVFEGASFVDGIFVITPEDEIPFCRDHVVERYGFTKVRGIVAGG
AERQHSVLNGLRAMEGTVADDDVILIHDGVRPFVSTDVLARATAVAREDDGALVAVPAKDTVKTVEDGIITGTPPRETLW
LAQTPQAFRYAVIRAAHEIADAERFLGTDDAMLVERLGRSVRIVVGDYRNIKITTPEDMVLAEAFLKELAA

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=236, Percent_Identity=28.8135593220339, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI157671913, Length=133, Percent_Identity=30.8270676691729, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI1789104, Length=235, Percent_Identity=34.8936170212766, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_GEOSL (Q746Z9)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_954408.1
- ProteinModelPortal:   Q746Z9
- SMR:   Q746Z9
- GeneID:   2688221
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU3368
- NMPDR:   fig|243231.1.peg.3346
- TIGR:   GSU3368
- HOGENOM:   HBG672839
- OMA:   PSNIKVT
- ProtClustDB:   PRK00155
- BioCyc:   GSUL243231:GSU_3368-MONOMER
- BRENDA:   2.7.7.60
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25084; Mature: 24953

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVFALVPAAGMGKRMGASINKQYLILAGRPILAHTLSVFEGASFVDGIFVITPEDEIPF
CEEEEEECCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCHHHCEEEEEECCCCCCH
CRDHVVERYGFTKVRGIVAGGAERQHSVLNGLRAMEGTVADDDVILIHDGVRPFVSTDVL
HHHHHHHHHCCHHHHHEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHH
ARATAVAREDDGALVAVPAKDTVKTVEDGIITGTPPRETLWLAQTPQAFRYAVIRAAHEI
HHHHHHEECCCCCEEEECCCHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
ADAERFLGTDDAMLVERLGRSVRIVVGDYRNIKITTPEDMVLAEAFLKELAA
HHHHHHCCCCHHHHHHHCCCEEEEEEECCCEEEEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AVFALVPAAGMGKRMGASINKQYLILAGRPILAHTLSVFEGASFVDGIFVITPEDEIPF
EEEEEECCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCHHHCEEEEEECCCCCCH
CRDHVVERYGFTKVRGIVAGGAERQHSVLNGLRAMEGTVADDDVILIHDGVRPFVSTDVL
HHHHHHHHHCCHHHHHEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHH
ARATAVAREDDGALVAVPAKDTVKTVEDGIITGTPPRETLWLAQTPQAFRYAVIRAAHEI
HHHHHHEECCCCCEEEECCCHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
ADAERFLGTDDAMLVERLGRSVRIVVGDYRNIKITTPEDMVLAEAFLKELAA
HHHHHHCCCCHHHHHHHCCCEEEEEEECCCEEEEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA