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The map label for this gene is rlmI [H]

Identifier: 39998234

GI number: 39998234

Start: 3451680

End: 3452888

Strand: Direct

Name: rlmI [H]

Synonym: GSU3144

Alternate gene names: 39998234

Gene position: 3451680-3452888 (Clockwise)

Preceding gene: 39998233

Following gene: 39998246

Centisome position: 90.5

GC content: 64.43

Gene sequence:

>1209_bases
ATGGGAGCTCTACGCTGCGTCGTCGGCCCTGAATCGGTGAGAATGCTGCAGTTGGGGCATCCCTGGATCATAGCCGACGC
CTATACGAAAAAGTGGCCCGCCGGCTCGGCCGGCGATCTGGTGGAACTGGTGGATGCCTCGGGGCGTTTTCTCGCAACGG
CCCTGCTGGACCCGGGCGAGCGCATTGTGGCGCGGGTACTGGGAGGGAAAGGACTCCGCCTTGGAGAAAGGTGGCTGGCA
CAACGGTTGGAAGACGCCCTGGAGCTGCGGCGGTCCCATGTCCCCCTTGAGGAGACCGATGCCTACCGGCTTGTGAACGG
CGAAGGGGACGGTCTGCCGGGCATCACGGTGGACCGCTACGGCGACTACCTGATGGTTCAGCTTTACTGCGGGGGGTGGC
GACCGCACCTGCCGGAACTGACCCGGGTGCTGGCCGGGGCCCTGAAACCGGCCGGCATCTATGAAAAAACCCGCCCCCGC
AACACGCGGGAGCTGGAAGCGGTTAGCGACACCAAGCGTTATGGGCGGCTCCTGGCCGGGACATCCGCACCGCCACGGCT
GCCCGTGCGGGAAAACGGTCTGACCTTTCTGGTCGAACTGGAGCGGGGTCTGAACACCGGCCTTTTCCTGGACCAGCGCG
CCAACCGCCGCCAGTTAATGGCCCGGACCGCCGGCAAGCGGGTGCTCAATCTCTTCGCCTATACCGGCGCCTTTTCCGTG
GCAGCCGCGGCGGGCGGAGCGAGCCGCGTGACAAGCGTGGATGCCTCTCCCACCTACACCGACTGGGCAAAGGCCCATTT
CGAGGCAAACCGCCTCAATCCCAAGCGCCACGAATTCATTGTGGGCGACTGCATGGACACCCTGGCGGAGCTCGCCCGCC
GGCAGGAGCGTTTCGACATCGTCCTGATGGACCCCCCTTCCTTCTCAACCACGTCGAAAAGCCGCTTCACCACGCGCGGC
GGCACCTCTGACCTTGTTGCCGCTTCGCTTCCCCTGCTGGCGGAAGGTGGGCTCCTGATCACCTCATCCAACCACCAGAA
GGTGGATATGGCCGACTACCTCAAGGAATTGCGCCGCGGAGCGCTCCAGGCAGGCAGCACCGTGCGCGTGATCTCCCTGG
CCGGCCAGCCTGAAGATTTCCCCTACCCGGTCACTTTTCCCGAAGGGCGCTATCTGAAATATGTCGTCAGTGTCAAGGGA
AGGACGTAA

Upstream 100 bases:

>100_bases
GCCTGAGCGGGCTATGGTCCGGCAACGGTGACCGCTCGATTAATTCGTTGCCCGCCGTCGGCAGCTGTGTTAACTGTTTC
ACCGGAACAAGGAGAAGACG

Downstream 100 bases:

>100_bases
CGGGTGCGTTACGTCCCGTGGGCTATCGCGGGGCAAGAGAACCGGCGGGTGCAGGACCGACAGGGCACATGAAAAGTCGC
ACCCAGAAGGGGATTGCGGG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MGALRCVVGPESVRMLQLGHPWIIADAYTKKWPAGSAGDLVELVDASGRFLATALLDPGERIVARVLGGKGLRLGERWLA
QRLEDALELRRSHVPLEETDAYRLVNGEGDGLPGITVDRYGDYLMVQLYCGGWRPHLPELTRVLAGALKPAGIYEKTRPR
NTRELEAVSDTKRYGRLLAGTSAPPRLPVRENGLTFLVELERGLNTGLFLDQRANRRQLMARTAGKRVLNLFAYTGAFSV
AAAAGGASRVTSVDASPTYTDWAKAHFEANRLNPKRHEFIVGDCMDTLAELARRQERFDIVLMDPPSFSTTSKSRFTTRG
GTSDLVAASLPLLAEGGLLITSSNHQKVDMADYLKELRRGALQAGSTVRVISLAGQPEDFPYPVTFPEGRYLKYVVSVKG
RT

Sequences:

>Translated_402_residues
MGALRCVVGPESVRMLQLGHPWIIADAYTKKWPAGSAGDLVELVDASGRFLATALLDPGERIVARVLGGKGLRLGERWLA
QRLEDALELRRSHVPLEETDAYRLVNGEGDGLPGITVDRYGDYLMVQLYCGGWRPHLPELTRVLAGALKPAGIYEKTRPR
NTRELEAVSDTKRYGRLLAGTSAPPRLPVRENGLTFLVELERGLNTGLFLDQRANRRQLMARTAGKRVLNLFAYTGAFSV
AAAAGGASRVTSVDASPTYTDWAKAHFEANRLNPKRHEFIVGDCMDTLAELARRQERFDIVLMDPPSFSTTSKSRFTTRG
GTSDLVAASLPLLAEGGLLITSSNHQKVDMADYLKELRRGALQAGSTVRVISLAGQPEDFPYPVTFPEGRYLKYVVSVKG
RT
>Mature_401_residues
GALRCVVGPESVRMLQLGHPWIIADAYTKKWPAGSAGDLVELVDASGRFLATALLDPGERIVARVLGGKGLRLGERWLAQ
RLEDALELRRSHVPLEETDAYRLVNGEGDGLPGITVDRYGDYLMVQLYCGGWRPHLPELTRVLAGALKPAGIYEKTRPRN
TRELEAVSDTKRYGRLLAGTSAPPRLPVRENGLTFLVELERGLNTGLFLDQRANRRQLMARTAGKRVLNLFAYTGAFSVA
AAAGGASRVTSVDASPTYTDWAKAHFEANRLNPKRHEFIVGDCMDTLAELARRQERFDIVLMDPPSFSTTSKSRFTTRGG
TSDLVAASLPLLAEGGLLITSSNHQKVDMADYLKELRRGALQAGSTVRVISLAGQPEDFPYPVTFPEGRYLKYVVSVKGR
T

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=392, Percent_Identity=33.1632653061224, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI1787181, Length=289, Percent_Identity=31.8339100346021, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44136; Mature: 44005

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGALRCVVGPESVRMLQLGHPWIIADAYTKKWPAGSAGDLVELVDASGRFLATALLDPGE
CCCEEEEECCCCEEEEEECCCEEEEECCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCHH
RIVARVLGGKGLRLGERWLAQRLEDALELRRSHVPLEETDAYRLVNGEGDGLPGITVDRY
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHCC
GDYLMVQLYCGGWRPHLPELTRVLAGALKPAGIYEKTRPRNTRELEAVSDTKRYGRLLAG
CCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
TSAPPRLPVRENGLTFLVELERGLNTGLFLDQRANRRQLMARTAGKRVLNLFAYTGAFSV
CCCCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AAAAGGASRVTSVDASPTYTDWAKAHFEANRLNPKRHEFIVGDCMDTLAELARRQERFDI
HHHCCCCHHEEECCCCCCCCHHHHHHHHHCCCCCHHHHEEHHHHHHHHHHHHHHHHHCEE
VLMDPPSFSTTSKSRFTTRGGTSDLVAASLPLLAEGGLLITSSNHQKVDMADYLKELRRG
EEECCCCCCCCCCCCEEECCCCCHHHHHCCCEEECCCEEEECCCCCEECHHHHHHHHHHH
ALQAGSTVRVISLAGQPEDFPYPVTFPEGRYLKYVVSVKGRT
HHCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCC
>Mature Secondary Structure 
GALRCVVGPESVRMLQLGHPWIIADAYTKKWPAGSAGDLVELVDASGRFLATALLDPGE
CCEEEEECCCCEEEEEECCCEEEEECCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCHH
RIVARVLGGKGLRLGERWLAQRLEDALELRRSHVPLEETDAYRLVNGEGDGLPGITVDRY
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHCC
GDYLMVQLYCGGWRPHLPELTRVLAGALKPAGIYEKTRPRNTRELEAVSDTKRYGRLLAG
CCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
TSAPPRLPVRENGLTFLVELERGLNTGLFLDQRANRRQLMARTAGKRVLNLFAYTGAFSV
CCCCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AAAAGGASRVTSVDASPTYTDWAKAHFEANRLNPKRHEFIVGDCMDTLAELARRQERFDI
HHHCCCCHHEEECCCCCCCCHHHHHHHHHCCCCCHHHHEEHHHHHHHHHHHHHHHHHCEE
VLMDPPSFSTTSKSRFTTRGGTSDLVAASLPLLAEGGLLITSSNHQKVDMADYLKELRRG
EEECCCCCCCCCCCCEEECCCCCHHHHHCCCEEECCCEEEECCCCCEECHHHHHHHHHHH
ALQAGSTVRVISLAGQPEDFPYPVTFPEGRYLKYVVSVKGRT
HHCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA