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The map label for this gene is cobI [H]

Identifier: 39998086

GI number: 39998086

Start: 3289047

End: 3289757

Strand: Reverse

Name: cobI [H]

Synonym: GSU2995

Alternate gene names: 39998086

Gene position: 3289757-3289047 (Counterclockwise)

Preceding gene: 39998087

Following gene: 39998085

Centisome position: 86.25

GC content: 64.7

Gene sequence:

>711_bases
GTGAGCGGTTTCGCCAAAATTTACGCCGTCGGGGTCGGCCCCGGCGACCCGGAACTCCTGACCCGGAAGGCCGAGCGGAT
CATCCGCTCGGCCGATGTCATCTGTGCCCCCACCGGCGCCGCCGATGCCGCCAGCTATGCCCTTTCCATCGTGGAGGACC
TCATCGATCCCTCCCGTCAGGAGGTGCTGCCCCTGGTCTTTCCCATGCGCAAGGACCAGGAGGGGCTCGACGCCTTCTGG
GAAGATGCGGCCGCCCAGGTGGCCGAGCGGGTCCGGCACGGCAGGGATGTGGCGTTCATCACCATCGGCGATCCGTTTCT
CTACTCCACGTTCCTCTACCTCTACCGTATCTTCCGGGAGTGCTATCCGGAAATAGCCGTTGAGATTGTGCCGGGAATTT
CCAGCATTACTGCCGCCGCCGCGGTTGCCGGTGTCCCCTTGGGGATGGCTGCCGAGCGGATCGCCATCCTCCCCACCACC
TACGAGGATGCGGACCTGCGCGAAACCTTCGCCCGGTACGACACCGTGGTCCTTATGAAGGTCAACCGGGTCTTCGACCG
GGTCTACGCCCTGCTGAAGGAGCTGGGGCTGGAGCGGTGCGGGGTGTTCGTGCGCCGGGTCGGTTCTGCCGACGAGGAGG
TGGTGTTCGATCTGGAGCGGCTCGTGGGGGAGAAGCTCGACTACCTGTCGCTGCTGATTGTCAGGAAGTAG

Upstream 100 bases:

>100_bases
TGGCGTGCGTCAACGTGGCCAAGACCCGAGGGCTCACCGAGTACAAGATGTTCGAGGCCCAGAATCCGGTCTACATCATC
TCGGCATGGAAAGGCGAAGA

Downstream 100 bases:

>100_bases
CACCGAACGCCATTGGAAAGGTTGTTCCCCGTGTCATCTGTCGCTGATAACCCCATTGTCCACTTCGTCGGCGCCGGCCC
CGGCGATGCCGAATTGATCA

Product: precorrin-2 C20-methyltransferase

Products: NA

Alternate protein names: S-adenosyl-L-methionine--cobalt-precorrin-2 methyltransferase [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MSGFAKIYAVGVGPGDPELLTRKAERIIRSADVICAPTGAADAASYALSIVEDLIDPSRQEVLPLVFPMRKDQEGLDAFW
EDAAAQVAERVRHGRDVAFITIGDPFLYSTFLYLYRIFRECYPEIAVEIVPGISSITAAAAVAGVPLGMAAERIAILPTT
YEDADLRETFARYDTVVLMKVNRVFDRVYALLKELGLERCGVFVRRVGSADEEVVFDLERLVGEKLDYLSLLIVRK

Sequences:

>Translated_236_residues
MSGFAKIYAVGVGPGDPELLTRKAERIIRSADVICAPTGAADAASYALSIVEDLIDPSRQEVLPLVFPMRKDQEGLDAFW
EDAAAQVAERVRHGRDVAFITIGDPFLYSTFLYLYRIFRECYPEIAVEIVPGISSITAAAAVAGVPLGMAAERIAILPTT
YEDADLRETFARYDTVVLMKVNRVFDRVYALLKELGLERCGVFVRRVGSADEEVVFDLERLVGEKLDYLSLLIVRK
>Mature_235_residues
SGFAKIYAVGVGPGDPELLTRKAERIIRSADVICAPTGAADAASYALSIVEDLIDPSRQEVLPLVFPMRKDQEGLDAFWE
DAAAQVAERVRHGRDVAFITIGDPFLYSTFLYLYRIFRECYPEIAVEIVPGISSITAAAAVAGVPLGMAAERIAILPTTY
EDADLRETFARYDTVVLMKVNRVFDRVYALLKELGLERCGVFVRRVGSADEEVVFDLERLVGEKLDYLSLLIVRK

Specific function: Methylates cobalt-precorrin-2 at the C-20 position to produce cobalt-precorrin-3A in the anaerobic cobalamin biosynthesis pathway [H]

COG id: COG2243

COG function: function code H; Precorrin-2 methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR012382
- InterPro:   IPR006364
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.151 [H]

Molecular weight: Translated: 26126; Mature: 25995

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFAKIYAVGVGPGDPELLTRKAERIIRSADVICAPTGAADAASYALSIVEDLIDPSRQ
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCHH
EVLPLVFPMRKDQEGLDAFWEDAAAQVAERVRHGRDVAFITIGDPFLYSTFLYLYRIFRE
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHH
CYPEIAVEIVPGISSITAAAAVAGVPLGMAAERIAILPTTYEDADLRETFARYDTVVLMK
HHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHH
VNRVFDRVYALLKELGLERCGVFVRRVGSADEEVVFDLERLVGEKLDYLSLLIVRK
HHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGFAKIYAVGVGPGDPELLTRKAERIIRSADVICAPTGAADAASYALSIVEDLIDPSRQ
CCCEEEEEEECCCCCHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCHH
EVLPLVFPMRKDQEGLDAFWEDAAAQVAERVRHGRDVAFITIGDPFLYSTFLYLYRIFRE
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHH
CYPEIAVEIVPGISSITAAAAVAGVPLGMAAERIAILPTTYEDADLRETFARYDTVVLMK
HHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHH
VNRVFDRVYALLKELGLERCGVFVRRVGSADEEVVFDLERLVGEKLDYLSLLIVRK
HHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]