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The map label for this gene is cobM [H]

Identifier: 39998085

GI number: 39998085

Start: 3288222

End: 3289016

Strand: Reverse

Name: cobM [H]

Synonym: GSU2994

Alternate gene names: 39998085

Gene position: 3289016-3288222 (Counterclockwise)

Preceding gene: 39998086

Following gene: 39998084

Centisome position: 86.23

GC content: 69.31

Gene sequence:

>795_bases
GTGTCATCTGTCGCTGATAACCCCATTGTCCACTTCGTCGGCGCCGGCCCCGGCGATGCCGAATTGATCACCGTGAAGGG
TGCCCGGCTCCTGCGGGAGGCCAAGGTGGTGGTCTATGCCGGGAGCCTGGTGGACCGCGAACTGGTCCGCACCTACGCCC
CCGGCGCCGAGGTGCATGACTCCGCCGCCCTGACCCTGGAGGAGACCACGGCGCTCCTGGCCCGGGCCGTGGCCGACGGC
CGGCGGGCCGTGCGCCTCCACACCGGCGACCCCTCCATCTACGGGGCCATCCAGGAGCAGATGGTTGAACTGGACCGGCT
CGGCATCGGCTACGAGGTGGTTCCCGGCGTGACGAGCGCCTTTGCCGCAGCGGCATCCCTGAAGCAGGAGCTGACCCTGC
CGGAGGTATCACAAACGGTCATCATAACCAGGCTGGCCGGTCGGACCCCGGTGCCCGAGCGGGAGCGGCTGGCGGAGATC
GCCCGGATCGGGGCGACGCTCGTCATCTACCTGTCGGTCGGCATGATCGAGCAGGTGGTGGCGGAACTCCTCCAGGGGGC
CTACACGCCGGCTACCCCCGCGGCGGTGGTGTCCCGGGCGTCCTGGCCCGACGAGCAGGCGGTGGAGGGAACCCTTGCCG
ACATCGCCGACAAGGTGCGGAGCTCCGGCATCGGCAAACAGGCGATCATCCTCGTGGGGGACGTGCTGAAGGCGCGCCGG
GAAGGGCTGAAGGCCCGGTCGCTCCTGTACGACGGCGGATTCTCGCACGAGTTCCGCACGGGGATTATCACGTAG

Upstream 100 bases:

>100_bases
GGTGTTCGATCTGGAGCGGCTCGTGGGGGAGAAGCTCGACTACCTGTCGCTGCTGATTGTCAGGAAGTAGCACCGAACGC
CATTGGAAAGGTTGTTCCCC

Downstream 100 bases:

>100_bases
CATTTCCCGCGGCAAAGGAGGTTAGGCGTCATGCGCATCGCCATCATCGCCATAACCCGCAACGGGGCCCGGCTCGGCTC
CCAGCTGCGGGAGGGACTGG

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSSVADNPIVHFVGAGPGDAELITVKGARLLREAKVVVYAGSLVDRELVRTYAPGAEVHDSAALTLEETTALLARAVADG
RRAVRLHTGDPSIYGAIQEQMVELDRLGIGYEVVPGVTSAFAAAASLKQELTLPEVSQTVIITRLAGRTPVPERERLAEI
ARIGATLVIYLSVGMIEQVVAELLQGAYTPATPAAVVSRASWPDEQAVEGTLADIADKVRSSGIGKQAIILVGDVLKARR
EGLKARSLLYDGGFSHEFRTGIIT

Sequences:

>Translated_264_residues
MSSVADNPIVHFVGAGPGDAELITVKGARLLREAKVVVYAGSLVDRELVRTYAPGAEVHDSAALTLEETTALLARAVADG
RRAVRLHTGDPSIYGAIQEQMVELDRLGIGYEVVPGVTSAFAAAASLKQELTLPEVSQTVIITRLAGRTPVPERERLAEI
ARIGATLVIYLSVGMIEQVVAELLQGAYTPATPAAVVSRASWPDEQAVEGTLADIADKVRSSGIGKQAIILVGDVLKARR
EGLKARSLLYDGGFSHEFRTGIIT
>Mature_263_residues
SSVADNPIVHFVGAGPGDAELITVKGARLLREAKVVVYAGSLVDRELVRTYAPGAEVHDSAALTLEETTALLARAVADGR
RAVRLHTGDPSIYGAIQEQMVELDRLGIGYEVVPGVTSAFAAAASLKQELTLPEVSQTVIITRLAGRTPVPERERLAEIA
RIGATLVIYLSVGMIEQVVAELLQGAYTPATPAAVVSRASWPDEQAVEGTLADIADKVRSSGIGKQAIILVGDVLKARRE
GLKARSLLYDGGFSHEFRTGIIT

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=244, Percent_Identity=29.5081967213115, Blast_Score=89, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322922, Length=265, Percent_Identity=23.7735849056604, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 27947; Mature: 27816

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVADNPIVHFVGAGPGDAELITVKGARLLREAKVVVYAGSLVDRELVRTYAPGAEVHD
CCCCCCCCEEEEEECCCCCCEEEEECHHHHHHHCEEEEEECCHHHHHHHHHHCCCCCCCC
SAALTLEETTALLARAVADGRRAVRLHTGDPSIYGAIQEQMVELDRLGIGYEVVPGVTSA
CCEEEHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHH
FAAAASLKQELTLPEVSQTVIITRLAGRTPVPERERLAEIARIGATLVIYLSVGMIEQVV
HHHHHHHHHHCCCCCHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AELLQGAYTPATPAAVVSRASWPDEQAVEGTLADIADKVRSSGIGKQAIILVGDVLKARR
HHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
EGLKARSLLYDGGFSHEFRTGIIT
HCCHHHHHHCCCCCCCHHHCCCCC
>Mature Secondary Structure 
SSVADNPIVHFVGAGPGDAELITVKGARLLREAKVVVYAGSLVDRELVRTYAPGAEVHD
CCCCCCCEEEEEECCCCCCEEEEECHHHHHHHCEEEEEECCHHHHHHHHHHCCCCCCCC
SAALTLEETTALLARAVADGRRAVRLHTGDPSIYGAIQEQMVELDRLGIGYEVVPGVTSA
CCEEEHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHH
FAAAASLKQELTLPEVSQTVIITRLAGRTPVPERERLAEIARIGATLVIYLSVGMIEQVV
HHHHHHHHHHCCCCCHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AELLQGAYTPATPAAVVSRASWPDEQAVEGTLADIADKVRSSGIGKQAIILVGDVLKARR
HHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
EGLKARSLLYDGGFSHEFRTGIIT
HCCHHHHHHCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]