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The map label for this gene is pdhC [H]

Identifier: 39997530

GI number: 39997530

Start: 2670681

End: 2671937

Strand: Reverse

Name: pdhC [H]

Synonym: GSU2435

Alternate gene names: 39997530

Gene position: 2671937-2670681 (Counterclockwise)

Preceding gene: 39997531

Following gene: 39997529

Centisome position: 70.05

GC content: 69.13

Gene sequence:

>1257_bases
ATGGCAACCGACATCACCATGCCCAAGCTCTCCGACACCATGACCGAAGGCCGTCTCGTCGCCTGGAAGAAAGGGGTGGG
CGACCGGGTTGAGCGGGGCGACATCATCGCCGAGGTAGAGACCGACAAGGCCACCATGGAGCTGGAGGCGTTCGCCTCGG
GCGTTCTGGCGGAGCAGCGGGTGAAGCCCGGCGAGCTGGTGAACGTGGGCACGGTTATCGGCGTGATCGGCGGGGCGGAT
GAGGTTAAGCCGACGGAGAAGGCCGCAGCCGCCCCCCCGGAGCTGGCCGACTGGCAGCCGCCACCGGAGGCGCCGGCGAA
CGGGGCGGAGCCTGAGATCCCGGAGCGGGTGCTGGAGTTGCCCGAAGCATCGGCGCCGCCGGCTCCCCTGCCGCCGGGCG
ACGATACCAAGGCCTCTCCCGCCGTGCGCCGCCTAGCGCGCGAGAAGGGCATCGACCTGCACCAGGTGAGGGGGAGCGGC
CCCGAGGGGCGCATTCTGATGGAGGACCTGGATCAGGTTGCGGCGAATGAGGAGCCCCCGGCGGCGCAGGCAGGGCAGGT
CTCTGCGGGCGAATCTCCCGCTCCGCCCGAGGCCGAGCCTATGACGCGCATGCGGGGCGCCATCGCCCGGATCACGGCCG
AGGCGTGGCGCACCATCCCCCATTTCTACGAGACCGTCGAGATCGACATGAAGGAGGCCGGTGAGATCGTCCGGGAACTG
AAGGGGAGCGGCAACGCCGTGACCTACAACGACCTGGTGCTCAAGGCCGCCGCCCTGGCCCTGGTGCAGTTTCCCCGGAT
GAACGCCTCGTTCCGGGACGGAGGTGTCGTTGCTCACCGGGAGGTGAACATCGGCTTTGCCGTGGCCATGGAGGAGGGGC
TGCAGGTGCCGGTGGTGAAGGGGTGTCAGTCGCTGGCCCTGAAGGAGATCGCCCTCCAGACCGTGCGCCTGGCCGAGCGG
GCCCGGAGCGGCGCCATCACCCAGGAGGAGATTTCCGGCGGCACCTTCAGCGTTTCGAACCTGGGGATGTACGGCATCGA
TGAGTTTGCCGCCGTGATCATGCCGCCCCAGGCAGCGATCCTGGCCGTGGGTGCCGTGGCGGACCGGCCCGTGGTGCGGG
ATGGGCAGCTGGCGGTCGCCCGGACCATGCGGGCGACCCTTTCCTGCGACCATCGGGTGGTGGACGGGGCCTATGCGGCC
CAATTCCTGGGCGAATTGCGGAGGGTGCTGGAGAATCCGGTGCTGATGCTGGTATGA

Upstream 100 bases:

>100_bases
TTCGCGCAAGATTGAGAAGCTCTGCATTCCGCAACCGGAGACGATCGCCGCGGCGGTGCGGGAGACGCTGAGCGGAACGT
ACTGAGAAAGGGATATCCTC

Downstream 100 bases:

>100_bases
TGATGCGGTTTCCGGGCAGGGGGGGTGCCGTGAAGATCGTGGATCTCGCCGCCATGGAGTACGCCGAGGCCTTTGCCCTC
CAGGAGCGGCTCGCTGCCGA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MATDITMPKLSDTMTEGRLVAWKKGVGDRVERGDIIAEVETDKATMELEAFASGVLAEQRVKPGELVNVGTVIGVIGGAD
EVKPTEKAAAAPPELADWQPPPEAPANGAEPEIPERVLELPEASAPPAPLPPGDDTKASPAVRRLAREKGIDLHQVRGSG
PEGRILMEDLDQVAANEEPPAAQAGQVSAGESPAPPEAEPMTRMRGAIARITAEAWRTIPHFYETVEIDMKEAGEIVREL
KGSGNAVTYNDLVLKAAALALVQFPRMNASFRDGGVVAHREVNIGFAVAMEEGLQVPVVKGCQSLALKEIALQTVRLAER
ARSGAITQEEISGGTFSVSNLGMYGIDEFAAVIMPPQAAILAVGAVADRPVVRDGQLAVARTMRATLSCDHRVVDGAYAA
QFLGELRRVLENPVLMLV

Sequences:

>Translated_418_residues
MATDITMPKLSDTMTEGRLVAWKKGVGDRVERGDIIAEVETDKATMELEAFASGVLAEQRVKPGELVNVGTVIGVIGGAD
EVKPTEKAAAAPPELADWQPPPEAPANGAEPEIPERVLELPEASAPPAPLPPGDDTKASPAVRRLAREKGIDLHQVRGSG
PEGRILMEDLDQVAANEEPPAAQAGQVSAGESPAPPEAEPMTRMRGAIARITAEAWRTIPHFYETVEIDMKEAGEIVREL
KGSGNAVTYNDLVLKAAALALVQFPRMNASFRDGGVVAHREVNIGFAVAMEEGLQVPVVKGCQSLALKEIALQTVRLAER
ARSGAITQEEISGGTFSVSNLGMYGIDEFAAVIMPPQAAILAVGAVADRPVVRDGQLAVARTMRATLSCDHRVVDGAYAA
QFLGELRRVLENPVLMLV
>Mature_417_residues
ATDITMPKLSDTMTEGRLVAWKKGVGDRVERGDIIAEVETDKATMELEAFASGVLAEQRVKPGELVNVGTVIGVIGGADE
VKPTEKAAAAPPELADWQPPPEAPANGAEPEIPERVLELPEASAPPAPLPPGDDTKASPAVRRLAREKGIDLHQVRGSGP
EGRILMEDLDQVAANEEPPAAQAGQVSAGESPAPPEAEPMTRMRGAIARITAEAWRTIPHFYETVEIDMKEAGEIVRELK
GSGNAVTYNDLVLKAAALALVQFPRMNASFRDGGVVAHREVNIGFAVAMEEGLQVPVVKGCQSLALKEIALQTVRLAERA
RSGAITQEEISGGTFSVSNLGMYGIDEFAAVIMPPQAAILAVGAVADRPVVRDGQLAVARTMRATLSCDHRVVDGAYAAQ
FLGELRRVLENPVLMLV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=432, Percent_Identity=37.5, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI203098753, Length=453, Percent_Identity=33.3333333333333, Blast_Score=198, Evalue=8e-51,
Organism=Homo sapiens, GI203098816, Length=453, Percent_Identity=33.5540838852097, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI110671329, Length=440, Percent_Identity=30.2272727272727, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI260898739, Length=205, Percent_Identity=39.5121951219512, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI19923748, Length=247, Percent_Identity=33.6032388663968, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1786946, Length=426, Percent_Identity=27.9342723004695, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1786305, Length=417, Percent_Identity=31.4148681055156, Blast_Score=138, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17560088, Length=433, Percent_Identity=35.3348729792148, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17537937, Length=436, Percent_Identity=30.2752293577982, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25146366, Length=421, Percent_Identity=28.978622327791, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=300, Percent_Identity=29.6666666666667, Blast_Score=134, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6324258, Length=460, Percent_Identity=31.9565217391304, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6320352, Length=429, Percent_Identity=29.3706293706294, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6321632, Length=184, Percent_Identity=32.0652173913043, Blast_Score=63, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24582497, Length=288, Percent_Identity=35.4166666666667, Blast_Score=155, Evalue=7e-38,
Organism=Drosophila melanogaster, GI20129315, Length=288, Percent_Identity=35.4166666666667, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI18859875, Length=421, Percent_Identity=31.353919239905, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24645909, Length=251, Percent_Identity=32.6693227091633, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 44327; Mature: 44196

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATDITMPKLSDTMTEGRLVAWKKGVGDRVERGDIIAEVETDKATMELEAFASGVLAEQR
CCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHC
VKPGELVNVGTVIGVIGGADEVKPTEKAAAAPPELADWQPPPEAPANGAEPEIPERVLEL
CCCCCEEEHHHHHEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHC
PEASAPPAPLPPGDDTKASPAVRRLAREKGIDLHQVRGSGPEGRILMEDLDQVAANEEPP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHCCCCCC
AAQAGQVSAGESPAPPEAEPMTRMRGAIARITAEAWRTIPHFYETVEIDMKEAGEIVREL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHH
KGSGNAVTYNDLVLKAAALALVQFPRMNASFRDGGVVAHREVNIGFAVAMEEGLQVPVVK
CCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCEEEEEECCCCCCHHHH
GCQSLALKEIALQTVRLAERARSGAITQEEISGGTFSVSNLGMYGIDEFAAVIMPPQAAI
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEECCCCCCCCHHHHHHHHCCCCHHH
LAVGAVADRPVVRDGQLAVARTMRATLSCDHRVVDGAYAAQFLGELRRVLENPVLMLV
HEEHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure 
ATDITMPKLSDTMTEGRLVAWKKGVGDRVERGDIIAEVETDKATMELEAFASGVLAEQR
CCCCCCCCHHHHHCCCEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHC
VKPGELVNVGTVIGVIGGADEVKPTEKAAAAPPELADWQPPPEAPANGAEPEIPERVLEL
CCCCCEEEHHHHHEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHC
PEASAPPAPLPPGDDTKASPAVRRLAREKGIDLHQVRGSGPEGRILMEDLDQVAANEEPP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHCCCCCC
AAQAGQVSAGESPAPPEAEPMTRMRGAIARITAEAWRTIPHFYETVEIDMKEAGEIVREL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHH
KGSGNAVTYNDLVLKAAALALVQFPRMNASFRDGGVVAHREVNIGFAVAMEEGLQVPVVK
CCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCEEEEEECCCCCCHHHH
GCQSLALKEIALQTVRLAERARSGAITQEEISGGTFSVSNLGMYGIDEFAAVIMPPQAAI
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEECCCCCCCCHHHHHHHHCCCCHHH
LAVGAVADRPVVRDGQLAVARTMRATLSCDHRVVDGAYAAQFLGELRRVLENPVLMLV
HEEHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]