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The map label for this gene is lipB

Identifier: 39997529

GI number: 39997529

Start: 2669944

End: 2670627

Strand: Reverse

Name: lipB

Synonym: GSU2434

Alternate gene names: 39997529

Gene position: 2670627-2669944 (Counterclockwise)

Preceding gene: 39997530

Following gene: 39997528

Centisome position: 70.02

GC content: 70.18

Gene sequence:

>684_bases
ATGGAGTACGCCGAGGCCTTTGCCCTCCAGGAGCGGCTCGCTGCCGACGTGGCCGCCGGCCGCGCGGAGGAGACGCTGCT
CCTGCTGGAGCATCCGCCGGTCTATACCCTGGGCCGGCGCGGCGATGGCGGGAGTCTTCCGGACCCGTCGGTGCGCCCCG
TGGAGATCAACCGGGGCGGCGACGTGACCTGGCACGGCCCCGGCCAGCTGGTGGGCTACCCCATCCTGGACCTGGGATGC
CGGGGCCGGGATCTGCACCGGTACCTCCGTTTTCTGGAACAGGTGCTGATGGACGCGGCCGCGTCTTTGGGCGTGCAGGC
CTGGCGGGTAGCCGGCCGGACCGGCATCTGGACCGAGGGGGGAAAGCTGGCCTCCATCGGGGTCGGGGTCCGGCGCTGGG
TTACCATGCACGGCTTCGCCCTCAACATCTGCAACGATCTGGCCCCCTTCAGCCGCATCCACCCCTGCGGCATCGTGGGC
TGCCCGGTCACCACCCTGTCTCGTGAAGCGGGCCGGGCGATCACCGTGGCCGAGGCCAAGGCCGCGGTTGCCGCCCCCTT
TGCCGGGCTGCCGGCCGACGCCCTGCCGGAGCAGCCCCGTGATGCCGTTCAACCTTCGTCATGCGATGATGTTCATGCAC
CGTCAACCACTTCAAGGAGGCCACCGTGTCCACTGACCGTCTGA

Upstream 100 bases:

>100_bases
GCGAATTGCGGAGGGTGCTGGAGAATCCGGTGCTGATGCTGGTATGATGATGCGGTTTCCGGGCAGGGGGGGTGCCGTGA
AGATCGTGGATCTCGCCGCC

Downstream 100 bases:

>100_bases
AACTCCCCGTCGAGAAGCTCCGCTGGATCTGCGATCCAGATCAGTTCGAATTCACCACCACCGATGATCTGCCCGAGCTG
GAGGGGACCCTCGGCCAGGC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MEYAEAFALQERLAADVAAGRAEETLLLLEHPPVYTLGRRGDGGSLPDPSVRPVEINRGGDVTWHGPGQLVGYPILDLGC
RGRDLHRYLRFLEQVLMDAAASLGVQAWRVAGRTGIWTEGGKLASIGVGVRRWVTMHGFALNICNDLAPFSRIHPCGIVG
CPVTTLSREAGRAITVAEAKAAVAAPFAGLPADALPEQPRDAVQPSSCDDVHAPSTTSRRPPCPLTV

Sequences:

>Translated_227_residues
MEYAEAFALQERLAADVAAGRAEETLLLLEHPPVYTLGRRGDGGSLPDPSVRPVEINRGGDVTWHGPGQLVGYPILDLGC
RGRDLHRYLRFLEQVLMDAAASLGVQAWRVAGRTGIWTEGGKLASIGVGVRRWVTMHGFALNICNDLAPFSRIHPCGIVG
CPVTTLSREAGRAITVAEAKAAVAAPFAGLPADALPEQPRDAVQPSSCDDVHAPSTTSRRPPCPLTV
>Mature_227_residues
MEYAEAFALQERLAADVAAGRAEETLLLLEHPPVYTLGRRGDGGSLPDPSVRPVEINRGGDVTWHGPGQLVGYPILDLGC
RGRDLHRYLRFLEQVLMDAAASLGVQAWRVAGRTGIWTEGGKLASIGVGVRRWVTMHGFALNICNDLAPFSRIHPCGIVG
CPVTTLSREAGRAITVAEAKAAVAAPFAGLPADALPEQPRDAVQPSSCDDVHAPSTTSRRPPCPLTV

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=203, Percent_Identity=38.423645320197, Blast_Score=89, Evalue=2e-18,
Organism=Escherichia coli, GI87081767, Length=149, Percent_Identity=38.9261744966443, Blast_Score=107, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6323268, Length=201, Percent_Identity=30.3482587064677, Blast_Score=89, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21355483, Length=219, Percent_Identity=36.986301369863, Blast_Score=128, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_GEOSL (Q74AE2)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_953480.1
- ProteinModelPortal:   Q74AE2
- SMR:   Q74AE2
- GeneID:   2687979
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU2434
- NMPDR:   fig|243231.1.peg.2418
- TIGR:   GSU2434
- HOGENOM:   HBG716160
- OMA:   HIEMSEF
- ProtClustDB:   CLSK828834
- BioCyc:   GSUL243231:GSU_2434-MONOMER
- BRENDA:   2.3.1.181
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24160; Mature: 24160

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYAEAFALQERLAADVAAGRAEETLLLLEHPPVYTLGRRGDGGSLPDPSVRPVEINRGG
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCEEEECCCC
DVTWHGPGQLVGYPILDLGCRGRDLHRYLRFLEQVLMDAAASLGVQAWRVAGRTGIWTEG
CEEECCCCCEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEECC
GKLASIGVGVRRWVTMHGFALNICNDLAPFSRIHPCGIVGCPVTTLSREAGRAITVAEAK
CEEEEHHHHHHHHHHHCCHHHHHHHHHCHHHHCCCCCEECCCHHHHHHHCCCEEEEEHHH
AAVAAPFAGLPADALPEQPRDAVQPSSCDDVHAPSTTSRRPPCPLTV
HHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEYAEAFALQERLAADVAAGRAEETLLLLEHPPVYTLGRRGDGGSLPDPSVRPVEINRGG
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCEEEECCCC
DVTWHGPGQLVGYPILDLGCRGRDLHRYLRFLEQVLMDAAASLGVQAWRVAGRTGIWTEG
CEEECCCCCEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEECC
GKLASIGVGVRRWVTMHGFALNICNDLAPFSRIHPCGIVGCPVTTLSREAGRAITVAEAK
CEEEEHHHHHHHHHHHCCHHHHHHHHHCHHHHCCCCCEECCCHHHHHHHCCCEEEEEHHH
AAVAAPFAGLPADALPEQPRDAVQPSSCDDVHAPSTTSRRPPCPLTV
HHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA