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The map label for this gene is folC [H]

Identifier: 39997464

GI number: 39997464

Start: 2595766

End: 2596911

Strand: Reverse

Name: folC [H]

Synonym: GSU2368

Alternate gene names: 39997464

Gene position: 2596911-2595766 (Counterclockwise)

Preceding gene: 39997466

Following gene: 308513319

Centisome position: 68.09

GC content: 69.37

Gene sequence:

>1146_bases
GTGGCCGGGACCAATGGCAAGGGGTCAACGGCCGCCTTTCTGGCCTCCATTCTGGCGGAAGGGGGATACCGGGCAGGACT
TTTCACCTCTCCCCATCTTACCCGCTTCACCGAACGGTTCAGGATCAACGGCACGGAGATCGCCGAGGAGGCCGCCGCAC
GCGTGGCCGGGCAGGTCCTGGCGAGCGCGCCGGAGGGCACCACCTTTTTCGAGGTGGTGACGGCCATGGCGCTGCTCCAT
TTCGTCGAGGAAGGGGTTGACGCAGCCATTCTCGAGGCGGGAATGGGGGGCGGCGCCGATGCCACGAGCGCTGCCACGGG
AATCCTGACGGTCATCGCTCCCGTTGCCCTCGATCATTGCGAGTGGCTGGGCGAGACCATCCCGGCCATCGCCCGGGAGA
AGGCCGGACTCATCAGGGCCGGACGGCCTGTGGTCCTCTCCCGGCAGCCGGCTGAAGCCCGTGACGTCTTCATTGAGCGC
GGTCGCATCCTGGGGGCCCCCCTGGTATGCTTCGGTGCTGATTTTTCCGCCGCGTGGGACGAATCGTCCCTTGCCTATGA
CGGGCTGTCGCGGCAGCTCGCCGGCCTGCGGCCCGGCATCGGCGGCCGCTATCAGCTGGCGAACGCAGCCACGGCCCTGG
CCGCCGCGGAAATCCTGGCCGACAACGGCTTCCGCCTCGATTCCCCCGCGTTGCGGGCAGGCATTGACGCGGCCCGCTGG
CCCGGCAGGATGGAACTCTTCTCCGGCGCGCCGCGGGTGCTGCTGGACGGCGCCCACAATCCCGACGGCGCCCGGGCGCT
CGCCGAGTCGCTGGCCGACTATCCCCGACGGCGTCTGATCGTGGTGACCGGCGTCATGGCGGACAAGGATGCGGCGGGGA
TTCTCGCACCTGTTCTGCCCCTGGCCCATGAGGTGGTGGCGGTCACTCCGGCGGTGGAACGGGGCCTTGATGCCGGCCGG
CTCGCCGAGCTGTGCCGGGAGAAGGGGCTGAATGCCGTTGCCGGCGGCACCGTCGCCGAGGGGCTCGCCCTCGCCCGGCA
GCGGGCCGGCGAGCAGGATCTCATTCTCGTCTGCGGTTCCCTCTTCACGGTGGGAGAGGCCCGGGCGATTCTTCTTTCGC
AGCAATACGAGCCGTTTCGCGGCTGA

Upstream 100 bases:

>100_bases
TTACGGTCTGCGGCGCTTCGGCATCAGGCCGGGACTCGAACGGATCGCCACTCTCCTCCAGCGGCTCGGTAACCCCCAGG
AGCGGCTCAGGGTCGTTCAT

Downstream 100 bases:

>100_bases
CCGACAACCGGGCATGCCGCTGATGTGCAGCTAACGACCCGGACACGGAAACCGTCCATGCCATTGAATCCCATCCGCAT
AGTTGGAGGCATCGTTCTCG

Product: folC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MAGTNGKGSTAAFLASILAEGGYRAGLFTSPHLTRFTERFRINGTEIAEEAAARVAGQVLASAPEGTTFFEVVTAMALLH
FVEEGVDAAILEAGMGGGADATSAATGILTVIAPVALDHCEWLGETIPAIAREKAGLIRAGRPVVLSRQPAEARDVFIER
GRILGAPLVCFGADFSAAWDESSLAYDGLSRQLAGLRPGIGGRYQLANAATALAAAEILADNGFRLDSPALRAGIDAARW
PGRMELFSGAPRVLLDGAHNPDGARALAESLADYPRRRLIVVTGVMADKDAAGILAPVLPLAHEVVAVTPAVERGLDAGR
LAELCREKGLNAVAGGTVAEGLALARQRAGEQDLILVCGSLFTVGEARAILLSQQYEPFRG

Sequences:

>Translated_381_residues
MAGTNGKGSTAAFLASILAEGGYRAGLFTSPHLTRFTERFRINGTEIAEEAAARVAGQVLASAPEGTTFFEVVTAMALLH
FVEEGVDAAILEAGMGGGADATSAATGILTVIAPVALDHCEWLGETIPAIAREKAGLIRAGRPVVLSRQPAEARDVFIER
GRILGAPLVCFGADFSAAWDESSLAYDGLSRQLAGLRPGIGGRYQLANAATALAAAEILADNGFRLDSPALRAGIDAARW
PGRMELFSGAPRVLLDGAHNPDGARALAESLADYPRRRLIVVTGVMADKDAAGILAPVLPLAHEVVAVTPAVERGLDAGR
LAELCREKGLNAVAGGTVAEGLALARQRAGEQDLILVCGSLFTVGEARAILLSQQYEPFRG
>Mature_380_residues
AGTNGKGSTAAFLASILAEGGYRAGLFTSPHLTRFTERFRINGTEIAEEAAARVAGQVLASAPEGTTFFEVVTAMALLHF
VEEGVDAAILEAGMGGGADATSAATGILTVIAPVALDHCEWLGETIPAIAREKAGLIRAGRPVVLSRQPAEARDVFIERG
RILGAPLVCFGADFSAAWDESSLAYDGLSRQLAGLRPGIGGRYQLANAATALAAAEILADNGFRLDSPALRAGIDAARWP
GRMELFSGAPRVLLDGAHNPDGARALAESLADYPRRRLIVVTGVMADKDAAGILAPVLPLAHEVVAVTPAVERGLDAGRL
AELCREKGLNAVAGGTVAEGLALARQRAGEQDLILVCGSLFTVGEARAILLSQQYEPFRG

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=355, Percent_Identity=27.0422535211268, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI66932984, Length=355, Percent_Identity=27.0422535211268, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1788654, Length=375, Percent_Identity=33.0666666666667, Blast_Score=178, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI71984923, Length=354, Percent_Identity=27.9661016949153, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17553148, Length=354, Percent_Identity=27.9661016949153, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17553150, Length=354, Percent_Identity=27.9661016949153, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323760, Length=395, Percent_Identity=28.8607594936709, Blast_Score=132, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6324815, Length=310, Percent_Identity=29.6774193548387, Blast_Score=118, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24641571, Length=271, Percent_Identity=32.1033210332103, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24581568, Length=172, Percent_Identity=30.2325581395349, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 39543; Mature: 39411

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTNGKGSTAAFLASILAEGGYRAGLFTSPHLTRFTERFRINGTEIAEEAAARVAGQVL
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ASAPEGTTFFEVVTAMALLHFVEEGVDAAILEAGMGGGADATSAATGILTVIAPVALDHC
HCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
EWLGETIPAIAREKAGLIRAGRPVVLSRQPAEARDVFIERGRILGAPLVCFGADFSAAWD
HHHHHHHHHHHHHHCCHHCCCCCEEEECCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCC
ESSLAYDGLSRQLAGLRPGIGGRYQLANAATALAAAEILADNGFRLDSPALRAGIDAARW
CCHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHCC
PGRMELFSGAPRVLLDGAHNPDGARALAESLADYPRRRLIVVTGVMADKDAAGILAPVLP
CCCHHHHCCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHH
LAHEVVAVTPAVERGLDAGRLAELCREKGLNAVAGGTVAEGLALARQRAGEQDLILVCGS
HHHHHHHCCHHHHHCCCHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCCCCCEEEEHHH
LFTVGEARAILLSQQYEPFRG
HHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AGTNGKGSTAAFLASILAEGGYRAGLFTSPHLTRFTERFRINGTEIAEEAAARVAGQVL
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ASAPEGTTFFEVVTAMALLHFVEEGVDAAILEAGMGGGADATSAATGILTVIAPVALDHC
HCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
EWLGETIPAIAREKAGLIRAGRPVVLSRQPAEARDVFIERGRILGAPLVCFGADFSAAWD
HHHHHHHHHHHHHHCCHHCCCCCEEEECCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCC
ESSLAYDGLSRQLAGLRPGIGGRYQLANAATALAAAEILADNGFRLDSPALRAGIDAARW
CCHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHCC
PGRMELFSGAPRVLLDGAHNPDGARALAESLADYPRRRLIVVTGVMADKDAAGILAPVLP
CCCHHHHCCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHH
LAHEVVAVTPAVERGLDAGRLAELCREKGLNAVAGGTVAEGLALARQRAGEQDLILVCGS
HHHHHHHCCHHHHHCCCHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCCCCCEEEEHHH
LFTVGEARAILLSQQYEPFRG
HHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]