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The map label for this gene is rpfG [H]

Identifier: 39997192

GI number: 39997192

Start: 2301233

End: 2302222

Strand: Reverse

Name: rpfG [H]

Synonym: GSU2094

Alternate gene names: 39997192

Gene position: 2302222-2301233 (Counterclockwise)

Preceding gene: 39997193

Following gene: 39997191

Centisome position: 60.36

GC content: 55.76

Gene sequence:

>990_bases
ATGAGTGTTAGTATCCTGTTTGTTGATGATGAAGAGCATATCCTTAAATCTCTGGTACGGCTTTTTGCCGACACGGGAAT
GGACGTGTTGCGGGCCGGGAGTGCGGTCGAGGCCCTGTCGCTCTTTGAACGGCACGAGATTGCAGTGGTCGTGGCAGACA
ACATGATGCCCGGTATGCGGGGCGTGGAGTTTTTCAGCCGGCTGAAAAACATTGCCCCCAAGACGGTCAATATCCTGATG
ACGTCCTATACCGACCTCCCCTCCGCCCTTGAGGCGATCAACCGCGGCGAGGTGTACCGGTTCGTGGTCAAACCGTGGGA
TAACGATGAGCTGGTGACGATGGTGGAGCAGTCAATACACCGCTATCTGACACTATCCACCCTTGCCCGTGAGGACGAGA
CGGTTATTCAGGGCCTTGCCCAGACCATCGAGTTGAAGGATCCCTACACCCGGGGCCACTGCGGCAGGGTTGCCTCCTAC
GCCCTGATGATTGCCGATGAACTGCGGCTTTCGCCGCAGATGCGCAAGGACCTCCGTTATGGCAGTTGGCTTCACGACTG
CGGCAAGATCGGGGTGCCCGAGGCTATTCTCAACTTCGGCGGTCCTGTGGCGGCACGCGAGTTCGACACCATAAAGAAGC
ATCCTGTAATGGGTGCTGAAATGGCCAGACAGGCACAGCTGCCTACGGCAGTCGTGAATATCGTGCTTTATCACCATGAA
CGCTTTGACGGAGAAGGCTATCCCCATCGGCTCGCCGGAGAGGCGATTCCCGTGGCGGCACGGATCGTCGCCGTTGCCGA
TGTTTATGATGCGCTGACGACTGAAAGGCCCTATCGTCGAGCCTATAGTTTGCAAAAAGGAGTTGAAATCCTCCTCTCAA
TGAAGGGAAATGCACTTGATCCCGCACTTGTGGATTGCTTCGTGGGAGCTCTCAATAAAAGAAATTTGCTGCTGAGCGAT
TGTGTGAGTGAAGACCGTGGCCACGGTTGA

Upstream 100 bases:

>100_bases
GTTGTCGTGAAATACTATCGCTGTGACTGGTTCCGACTCCGAACTCTGGCAATGGGAGAGGATGAGGGTGGGGGGCTGGT
TTCCGAATGGAGGGGTGTAC

Downstream 100 bases:

>100_bases
ATCTCCGAAACTGCCCCGGTCAGGGTGCATACCACCGCTCACGAGGAGCCTGTGCCAGAGCCTGTCACCGTGCAGCCGTC
CCGGTAAACGCTGCAACGCA

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSVSILFVDDEEHILKSLVRLFADTGMDVLRAGSAVEALSLFERHEIAVVVADNMMPGMRGVEFFSRLKNIAPKTVNILM
TSYTDLPSALEAINRGEVYRFVVKPWDNDELVTMVEQSIHRYLTLSTLAREDETVIQGLAQTIELKDPYTRGHCGRVASY
ALMIADELRLSPQMRKDLRYGSWLHDCGKIGVPEAILNFGGPVAAREFDTIKKHPVMGAEMARQAQLPTAVVNIVLYHHE
RFDGEGYPHRLAGEAIPVAARIVAVADVYDALTTERPYRRAYSLQKGVEILLSMKGNALDPALVDCFVGALNKRNLLLSD
CVSEDRGHG

Sequences:

>Translated_329_residues
MSVSILFVDDEEHILKSLVRLFADTGMDVLRAGSAVEALSLFERHEIAVVVADNMMPGMRGVEFFSRLKNIAPKTVNILM
TSYTDLPSALEAINRGEVYRFVVKPWDNDELVTMVEQSIHRYLTLSTLAREDETVIQGLAQTIELKDPYTRGHCGRVASY
ALMIADELRLSPQMRKDLRYGSWLHDCGKIGVPEAILNFGGPVAAREFDTIKKHPVMGAEMARQAQLPTAVVNIVLYHHE
RFDGEGYPHRLAGEAIPVAARIVAVADVYDALTTERPYRRAYSLQKGVEILLSMKGNALDPALVDCFVGALNKRNLLLSD
CVSEDRGHG
>Mature_328_residues
SVSILFVDDEEHILKSLVRLFADTGMDVLRAGSAVEALSLFERHEIAVVVADNMMPGMRGVEFFSRLKNIAPKTVNILMT
SYTDLPSALEAINRGEVYRFVVKPWDNDELVTMVEQSIHRYLTLSTLAREDETVIQGLAQTIELKDPYTRGHCGRVASYA
LMIADELRLSPQMRKDLRYGSWLHDCGKIGVPEAILNFGGPVAAREFDTIKKHPVMGAEMARQAQLPTAVVNIVLYHHER
FDGEGYPHRLAGEAIPVAARIVAVADVYDALTTERPYRRAYSLQKGVEILLSMKGNALDPALVDCFVGALNKRNLLLSDC
VSEDRGHG

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=163, Percent_Identity=31.9018404907975, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790299, Length=118, Percent_Identity=31.3559322033898, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 36583; Mature: 36451

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSILFVDDEEHILKSLVRLFADTGMDVLRAGSAVEALSLFERHEIAVVVADNMMPGMR
CEEEEEEECCHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHCCEEEEEECCCCCCHH
GVEFFSRLKNIAPKTVNILMTSYTDLPSALEAINRGEVYRFVVKPWDNDELVTMVEQSIH
HHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHH
RYLTLSTLAREDETVIQGLAQTIELKDPYTRGHCGRVASYALMIADELRLSPQMRKDLRY
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
GSWLHDCGKIGVPEAILNFGGPVAAREFDTIKKHPVMGAEMARQAQLPTAVVNIVLYHHE
CHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHH
RFDGEGYPHRLAGEAIPVAARIVAVADVYDALTTERPYRRAYSLQKGVEILLSMKGNALD
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC
PALVDCFVGALNKRNLLLSDCVSEDRGHG
HHHHHHHHHHCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
SVSILFVDDEEHILKSLVRLFADTGMDVLRAGSAVEALSLFERHEIAVVVADNMMPGMR
EEEEEEECCHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHCCEEEEEECCCCCCHH
GVEFFSRLKNIAPKTVNILMTSYTDLPSALEAINRGEVYRFVVKPWDNDELVTMVEQSIH
HHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHH
RYLTLSTLAREDETVIQGLAQTIELKDPYTRGHCGRVASYALMIADELRLSPQMRKDLRY
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
GSWLHDCGKIGVPEAILNFGGPVAAREFDTIKKHPVMGAEMARQAQLPTAVVNIVLYHHE
CHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHH
RFDGEGYPHRLAGEAIPVAARIVAVADVYDALTTERPYRRAYSLQKGVEILLSMKGNALD
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC
PALVDCFVGALNKRNLLLSDCVSEDRGHG
HHHHHHHHHHCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]