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The map label for this gene is nasB [H]

Identifier: 39997193

GI number: 39997193

Start: 2302816

End: 2304084

Strand: Reverse

Name: nasB [H]

Synonym: GSU2095

Alternate gene names: 39997193

Gene position: 2304084-2302816 (Counterclockwise)

Preceding gene: 39997194

Following gene: 39997192

Centisome position: 60.41

GC content: 67.53

Gene sequence:

>1269_bases
ATGAAGGTGGTAACCGTGGGGACGGGAATGGCGGCCGCCGAGTTCGTCGAGCGGCTCAGGCTCGACGGATTCGCGGGCGA
GATCGTCATGTGCGGTGACGAGGAATTTGCTCCCTATTCCCCCTGCGTCATCCCCTTCTACCTGGCCGGTGAGCCGCTGG
AGACCGTCTACTGGAAGGGGAGCGACTTCTACGGCCGCTACCGCGTGACGCCGCGCCTGGCCGACCCGGTGGTGGAGGTG
GATGCGGAGCGGCGGCTGGTGCGGACCGCATCGGGACGGAGCGAACAGTACGACCGCCTCTTCTACGCCGCTGGCGCCCG
CAGCTGGTATCCCCGGCCCGACTGGCTCGACACCCGGGGCGTCTTCGGTTTCAAGACTCTGTCGGACATGGTTGCCATCG
ACCGCTACATCCGCGAGCACAACGTGGGCGAGGCGGTCGTGTTCGGCGGCGGCTTCATCGGGGTGGACGCGGCCCTGGCC
CTGTGGCACCGCGGGCTCGCCATCACCCTGGTCCACCGCAACACCCGGGTCCTGTCCCAGATGACGGACGAAGAGGGGGG
GCAGTTCGCCACCGCGAAACTGGTGGAAAAGACCGGGATGGACATCCGGCTCAGGACCACGGTGGCCGACATCGTGACCA
CGGGGGGAGAGCTCTCAGCGGTCCGTTTCTCCGACGGCACCGCCAGGGAGACGAAGCTGCTGATCGTGGCAATCGGAGTC
TCCCCCAACTCGGAGCCCCTGCGCGGCGACGACAAGGGGGTGCCGTCGGACCGGCAGATGCTGGCGGAAGCGGGCATCTA
CGCAGCCGGGGACGTGGCCGTCACCCCCCATGCGGTCACCGGCGAGGCCGGGGTTTATGCCACCTATCCCAACGCCATGC
GCCAGGCCCGCACCGCCGCACGGCACCTGTTGAACGGCGACGGCAGCTACGCCGGCTCGGTCAATACGAACGTGCTCAAG
AAGCACATCGACTTTCCCATCGTCTCGGCGGGCAGCTTCACGGGCGAGCCGGTCACCTGGCAGCGGGGCGACGTCTGGCG
CCGGGTCTACCTGAAGGACGGCATGATCAACGGCTACATCATCATCGGCGACACGAAGATGTCGGGATACGTCTATCAGC
TCTACGTCTCGCGTCAGCGGGTCGACGGCACAATCCGCACCATCCTCTCCTCCCCGCGCCACGATGCATACTACCGGCAG
GTGACCGGATGCGGCGCCGGCAGGGCGTCCGGGCGGGGGTACGGGATGCAGGGCGTCATGGCCGGATAG

Upstream 100 bases:

>100_bases
TGCGGCACTACCTCCTTGGCAGGGAAGGACTGCCGCCCGGCCTCGAACTGCTGCGGGAGATGCGCCGGAAAGAGTTTGCC
CGGCGCCGCGGAGGTGAGTG

Downstream 100 bases:

>100_bases
CCTGTCGCCCCTCTGCACGCGGCGCCGTTGCGGTGCGAAGGGGGAGAGGGCAGATGCCTATTGCTACCGCTCAGGGACCG
GATTGCGAATCCGGTCCCTT

Product: NADH oxidase

Products: 3 NAD(P)+; NH4OH; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKVVTVGTGMAAAEFVERLRLDGFAGEIVMCGDEEFAPYSPCVIPFYLAGEPLETVYWKGSDFYGRYRVTPRLADPVVEV
DAERRLVRTASGRSEQYDRLFYAAGARSWYPRPDWLDTRGVFGFKTLSDMVAIDRYIREHNVGEAVVFGGGFIGVDAALA
LWHRGLAITLVHRNTRVLSQMTDEEGGQFATAKLVEKTGMDIRLRTTVADIVTTGGELSAVRFSDGTARETKLLIVAIGV
SPNSEPLRGDDKGVPSDRQMLAEAGIYAAGDVAVTPHAVTGEAGVYATYPNAMRQARTAARHLLNGDGSYAGSVNTNVLK
KHIDFPIVSAGSFTGEPVTWQRGDVWRRVYLKDGMINGYIIIGDTKMSGYVYQLYVSRQRVDGTIRTILSSPRHDAYYRQ
VTGCGAGRASGRGYGMQGVMAG

Sequences:

>Translated_422_residues
MKVVTVGTGMAAAEFVERLRLDGFAGEIVMCGDEEFAPYSPCVIPFYLAGEPLETVYWKGSDFYGRYRVTPRLADPVVEV
DAERRLVRTASGRSEQYDRLFYAAGARSWYPRPDWLDTRGVFGFKTLSDMVAIDRYIREHNVGEAVVFGGGFIGVDAALA
LWHRGLAITLVHRNTRVLSQMTDEEGGQFATAKLVEKTGMDIRLRTTVADIVTTGGELSAVRFSDGTARETKLLIVAIGV
SPNSEPLRGDDKGVPSDRQMLAEAGIYAAGDVAVTPHAVTGEAGVYATYPNAMRQARTAARHLLNGDGSYAGSVNTNVLK
KHIDFPIVSAGSFTGEPVTWQRGDVWRRVYLKDGMINGYIIIGDTKMSGYVYQLYVSRQRVDGTIRTILSSPRHDAYYRQ
VTGCGAGRASGRGYGMQGVMAG
>Mature_422_residues
MKVVTVGTGMAAAEFVERLRLDGFAGEIVMCGDEEFAPYSPCVIPFYLAGEPLETVYWKGSDFYGRYRVTPRLADPVVEV
DAERRLVRTASGRSEQYDRLFYAAGARSWYPRPDWLDTRGVFGFKTLSDMVAIDRYIREHNVGEAVVFGGGFIGVDAALA
LWHRGLAITLVHRNTRVLSQMTDEEGGQFATAKLVEKTGMDIRLRTTVADIVTTGGELSAVRFSDGTARETKLLIVAIGV
SPNSEPLRGDDKGVPSDRQMLAEAGIYAAGDVAVTPHAVTGEAGVYATYPNAMRQARTAARHLLNGDGSYAGSVNTNVLK
KHIDFPIVSAGSFTGEPVTWQRGDVWRRVYLKDGMINGYIIIGDTKMSGYVYQLYVSRQRVDGTIRTILSSPRHDAYYRQ
VTGCGAGRASGRGYGMQGVMAG

Specific function: Required for nitrate assimilation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI21389617, Length=310, Percent_Identity=26.1290322580645, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI65787454, Length=310, Percent_Identity=26.1290322580645, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI226437568, Length=310, Percent_Identity=26.1290322580645, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1789765, Length=330, Percent_Identity=22.7272727272727, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17559934, Length=282, Percent_Identity=24.822695035461, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24585130, Length=246, Percent_Identity=25.2032520325203, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24639257, Length=314, Percent_Identity=24.5222929936306, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281359715, Length=314, Percent_Identity=24.5222929936306, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281359713, Length=314, Percent_Identity=24.5222929936306, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24639250, Length=314, Percent_Identity=24.5222929936306, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI18543267, Length=314, Percent_Identity=24.5222929936306, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24639252, Length=314, Percent_Identity=24.5222929936306, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.7.1.4 [C]

Molecular weight: Translated: 46075; Mature: 46075

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVTVGTGMAAAEFVERLRLDGFAGEIVMCGDEEFAPYSPCVIPFYLAGEPLETVYWKG
CEEEEECCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCEEEEECC
SDFYGRYRVTPRLADPVVEVDAERRLVRTASGRSEQYDRLFYAAGARSWYPRPDWLDTRG
CCCCEEEEECCCCCCCEEEECHHHHHHHHCCCCCHHHHHEEEECCCCCCCCCCCCCCCCC
VFGFKTLSDMVAIDRYIREHNVGEAVVFGGGFIGVDAALALWHRGLAITLVHRNTRVLSQ
CCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCEEEEEEECCHHHHHH
MTDEEGGQFATAKLVEKTGMDIRLRTTVADIVTTGGELSAVRFSDGTARETKLLIVAIGV
HHCCCCCCCHHHHHHHHCCCCEEEEEEHHHHHCCCCCEEEEEECCCCCCCEEEEEEEEEC
SPNSEPLRGDDKGVPSDRQMLAEAGIYAAGDVAVTPHAVTGEAGVYATYPNAMRQARTAA
CCCCCCCCCCCCCCCHHHHHHHHCCCEECCCEEECCCEECCCCCEEEECHHHHHHHHHHH
RHLLNGDGSYAGSVNTNVLKKHIDFPIVSAGSFTGEPVTWQRGDVWRRVYLKDGMINGYI
HHHHCCCCCCCCCCCHHHHHHHCCCCEEECCCCCCCCCEECCCCCEEEEEEECCCCCCEE
IIGDTKMSGYVYQLYVSRQRVDGTIRTILSSPRHDAYYRQVTGCGAGRASGRGYGMQGVM
EEECCCCCCEEEEEEEEHHHHCHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCC
AG
CC
>Mature Secondary Structure
MKVVTVGTGMAAAEFVERLRLDGFAGEIVMCGDEEFAPYSPCVIPFYLAGEPLETVYWKG
CEEEEECCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCEEEEECC
SDFYGRYRVTPRLADPVVEVDAERRLVRTASGRSEQYDRLFYAAGARSWYPRPDWLDTRG
CCCCEEEEECCCCCCCEEEECHHHHHHHHCCCCCHHHHHEEEECCCCCCCCCCCCCCCCC
VFGFKTLSDMVAIDRYIREHNVGEAVVFGGGFIGVDAALALWHRGLAITLVHRNTRVLSQ
CCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCEEEEEEECCHHHHHH
MTDEEGGQFATAKLVEKTGMDIRLRTTVADIVTTGGELSAVRFSDGTARETKLLIVAIGV
HHCCCCCCCHHHHHHHHCCCCEEEEEEHHHHHCCCCCEEEEEECCCCCCCEEEEEEEEEC
SPNSEPLRGDDKGVPSDRQMLAEAGIYAAGDVAVTPHAVTGEAGVYATYPNAMRQARTAA
CCCCCCCCCCCCCCCHHHHHHHHCCCEECCCEEECCCEECCCCCEEEECHHHHHHHHHHH
RHLLNGDGSYAGSVNTNVLKKHIDFPIVSAGSFTGEPVTWQRGDVWRRVYLKDGMINGYI
HHHHCCCCCCCCCCCHHHHHHHCCCCEEECCCCCCCCCEECCCCCEEEEEEECCCCCCEE
IIGDTKMSGYVYQLYVSRQRVDGTIRTILSSPRHDAYYRQVTGCGAGRASGRGYGMQGVM
EEECCCCCCEEEEEEEEHHHHCHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCC
AG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD; NAD+; NADH; Siroheme [C]

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.4 {NO2-}} 0.005 {NO2-}} 0.016 {NADH}} 5.3 {hydroxylamine}} [C]

Substrates: 3 NAD(P)H; nitrite [C]

Specific reaction: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O [C]

General reaction: Redox reaction [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Bipyridine; Arsenite; CN-; NAD+; NADP+; NaN3; p-Chloromercuribenzoate; SO32- [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]