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The map label for this gene is pilD [H]

Identifier: 39997141

GI number: 39997141

Start: 2238751

End: 2239530

Strand: Reverse

Name: pilD [H]

Synonym: GSU2043

Alternate gene names: 39997141

Gene position: 2239530-2238751 (Counterclockwise)

Preceding gene: 39997142

Following gene: 39997140

Centisome position: 58.72

GC content: 59.23

Gene sequence:

>780_bases
ATGACGCTACCGATAGTGTTCTATCTTTTTTCCTTTGTCCTCGGGGCAGTGGTCGGCTCATTCCTGAACGTCTGCATTTA
TAGGCTGCCAACGGGTGAGTCGGTCGTGTTTCCTCCTTCCCGCTGCACCTCCTGCGGGACGCGGATTCGTCCCTGGGACA
ACATCCCTATTCTGAGCTGGCTCATCCTGCGCGGAGCATGCCGCGCCTGCAGGGCAAAGATCTCGGCTCGCTATCCCCTG
GTTGAACTTATCAACGGCTTGCTCTGCCTCGCCCTGTTCCTCAAGTTCGGGCCGACCCTGACCTTTGCGGCCCTCTTCGT
ATTCTGCTCCGCCCTGGTGGCGATCTCCTTCATTGACCTCGACCATCAGATCATCCCCGATGTCATCAGCCTCCCGGGAA
TCGTTCTTGGATTTGTCCTGTCGTTCTTTCTGCCCTGGCTCGGCTGGCTCAATTCCCTCATCGGCATCGCGGCGGGGGGA
GGGAGCCTCCTGCTGGTTGCCTGGCTCTACGAGCGGCTGACGGGCAAGGAGGGAATGGGCGGAGGTGACATCAAGCTTCT
TGCCATGATGGGGGCCTTTCTCGGCTGGCGGGCGGTGCCGTTCATCATCTTTGCCAGCTCTCTGGTGGGCTCGGTCATCG
GGCTCACACTCATGATGCTCCAGAAAAAAGATAGCAAGCTTGCCATCCCGTTCGGCCCGTTCCTGGCGCTGGGGGCACTT
CTCTACATCTTTTTCGGCAAGGCGATCATTCTCTGGTACCTGAGCATCGGCGCCCGGTAG

Upstream 100 bases:

>100_bases
GCCGGGCCGCGGGCGCCTACCTCGGTTTCGGAACCTGGTGCTGACGGGCGGGCGAAAGCAGGGCGACAAGGGCCGGGCTG
GCCGTCTTAACGGTTAATTT

Downstream 100 bases:

>100_bases
CGAGGTGCACCCGTGAGGCGTCTGCGCTTCAGTCTCAGCTTCCTGATCCTCTCTTCCCTTACGTTCCTCCTGGTTCTTAC
CTGGTTTCTCCTGAGCCTGA

Product: type 4 prepilin-like proteins leader peptide processing enzyme

Products: NA

Alternate protein names: Protein pilD; Protein secretion protein XCPA; Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MTLPIVFYLFSFVLGAVVGSFLNVCIYRLPTGESVVFPPSRCTSCGTRIRPWDNIPILSWLILRGACRACRAKISARYPL
VELINGLLCLALFLKFGPTLTFAALFVFCSALVAISFIDLDHQIIPDVISLPGIVLGFVLSFFLPWLGWLNSLIGIAAGG
GSLLLVAWLYERLTGKEGMGGGDIKLLAMMGAFLGWRAVPFIIFASSLVGSVIGLTLMMLQKKDSKLAIPFGPFLALGAL
LYIFFGKAIILWYLSIGAR

Sequences:

>Translated_259_residues
MTLPIVFYLFSFVLGAVVGSFLNVCIYRLPTGESVVFPPSRCTSCGTRIRPWDNIPILSWLILRGACRACRAKISARYPL
VELINGLLCLALFLKFGPTLTFAALFVFCSALVAISFIDLDHQIIPDVISLPGIVLGFVLSFFLPWLGWLNSLIGIAAGG
GSLLLVAWLYERLTGKEGMGGGDIKLLAMMGAFLGWRAVPFIIFASSLVGSVIGLTLMMLQKKDSKLAIPFGPFLALGAL
LYIFFGKAIILWYLSIGAR
>Mature_258_residues
TLPIVFYLFSFVLGAVVGSFLNVCIYRLPTGESVVFPPSRCTSCGTRIRPWDNIPILSWLILRGACRACRAKISARYPLV
ELINGLLCLALFLKFGPTLTFAALFVFCSALVAISFIDLDHQIIPDVISLPGIVLGFVLSFFLPWLGWLNSLIGIAAGGG
SLLLVAWLYERLTGKEGMGGGDIKLLAMMGAFLGWRAVPFIIFASSLVGSVIGLTLMMLQKKDSKLAIPFGPFLALGALL
YIFFGKAIILWYLSIGAR

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the pilin precursor during membrane translocation. Required for the assembly of type IV pili and for secretion of most proteins [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=261, Percent_Identity=33.7164750957854, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1789732, Length=146, Percent_Identity=36.986301369863, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 28147; Mature: 28016

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLPIVFYLFSFVLGAVVGSFLNVCIYRLPTGESVVFPPSRCTSCGTRIRPWDNIPILSW
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHCCCCCCCCCCCHHHHH
LILRGACRACRAKISARYPLVELINGLLCLALFLKFGPTLTFAALFVFCSALVAISFIDL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
DHQIIPDVISLPGIVLGFVLSFFLPWLGWLNSLIGIAAGGGSLLLVAWLYERLTGKEGMG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
GGDIKLLAMMGAFLGWRAVPFIIFASSLVGSVIGLTLMMLQKKDSKLAIPFGPFLALGAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHH
LYIFFGKAIILWYLSIGAR
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7905475 [H]