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The map label for this gene is nifS1 [H]

Identifier: 39997109

GI number: 39997109

Start: 2203783

End: 2204958

Strand: Reverse

Name: nifS1 [H]

Synonym: GSU2011

Alternate gene names: 39997109

Gene position: 2204958-2203783 (Counterclockwise)

Preceding gene: 39997110

Following gene: 39997102

Centisome position: 57.81

GC content: 60.46

Gene sequence:

>1176_bases
ATGAAGGAGATCTACCTGGACAACAACGCCACCACCAAGGTGGACGAGGCTGTTTTCGAGGAGATGCGTCCCTATTTCTG
TGACCTCTACGGCAACCCCAGCTCCATGCACTATTTCGGCGGTCAGGTCCAGAGGAAGGTGGACGAGGCCCGGAATCGGG
TCGCAGCGCTCCTGGGCGCCCTGCCCGAAGAGATCATCTTCACCGCCTGTGGTACCGAGAGCGACAACGCGGCCATCCGT
TCGGCCCTTGAAGTTTTCCCGGAGCGCCGCCACATCATTACCACCCGTGTGGAGCATCCGGCAGTCCTTACCCTCTGCCG
CAACCTGTCAAAGCGGGGGTACCGGGTCACCGAACTGGGTGTTGACGGGGAAGGCCGGCTTGACCTGAATGAACTGCGGA
GCGCGATTGACGAGGATACCGTCGTGGTATCAGTTATGTGGGCCAACAACGAAACAGGCGTGATCTTCCCGGTTGAGGAG
ATCAGCCGGATCGTCAAGGAGAAAGGAAAGGGGGCGCTGTTCCATACCGACGCGGTTCAGGCCGTGGGGAAAATACCCAT
CAACATGGCCACGTCGTCCATCGACATGCTTTCCATTTCGGGACACAAGCTCCATGCTCCTAAGGGTACGGGGGTTCTTT
ACCTGCGCAAAGGGGTACCGTTCAGGCCGTTCATGGTCGGCGGGCACCAGGAGCACAGTCGCCGGGCCGGCACCGAAAAT
ACCGCAGGCATCATCGCGCTCGGCAAGGCATGCGAACTGGCAGGACACTGGATGGAAGACGAAAATACGCGGGTCAAGGC
CCTGCGCGACCGGCTCGAGGCAGCGCTGCTCGAACTGATCCCCCGGGCCAGGATCAACGGGGGCGAAGCTGAGCGCCTCC
CCAATACCCTCTCCATTGCCTTCGAGTTTGTGGAAGGGGAGGCGATCCTTATGCTGATGTCCGAGAAGGGGATTTGTGCA
TCGTCGGGGAGCGCCTGCACCTCCGGCTCCCTTGAGCCGTCCCACGTGCTGCGGGCCATGGGGGTTCCGTTTACCTGTGC
CCACGGTTCCATCCGATTCTCGTTGTCTCGTTACACCACGGACGAAGAGATCGACACCATAATCCGCGAGTTGCCGCCCA
TCATCCGTCGGTTGCGGGAAATGTCGCCCTTTGGTCGGGAGTTTCTCAACGCCTGA

Upstream 100 bases:

>100_bases
CGTGTGCCGGGTGCGCCGCTTCGGGCAATACCGCCAAGTTCGTCGAGATGAAGCTGCGCGAGCTTGTGGCCGAGGATATC
ACTGTCACGGAGGTGGAGGG

Downstream 100 bases:

>100_bases
ACCGACCGACATTACAGAAGAAAAGGAAAAAGGGGGCCGTCACTGACGTGTCCCCTTTTTCCTTTTCAAGCGGTTTGTGA
GTTGTCTGGCCAGTCCGGGC

Product: cysteine desulfurase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MKEIYLDNNATTKVDEAVFEEMRPYFCDLYGNPSSMHYFGGQVQRKVDEARNRVAALLGALPEEIIFTACGTESDNAAIR
SALEVFPERRHIITTRVEHPAVLTLCRNLSKRGYRVTELGVDGEGRLDLNELRSAIDEDTVVVSVMWANNETGVIFPVEE
ISRIVKEKGKGALFHTDAVQAVGKIPINMATSSIDMLSISGHKLHAPKGTGVLYLRKGVPFRPFMVGGHQEHSRRAGTEN
TAGIIALGKACELAGHWMEDENTRVKALRDRLEAALLELIPRARINGGEAERLPNTLSIAFEFVEGEAILMLMSEKGICA
SSGSACTSGSLEPSHVLRAMGVPFTCAHGSIRFSLSRYTTDEEIDTIIRELPPIIRRLREMSPFGREFLNA

Sequences:

>Translated_391_residues
MKEIYLDNNATTKVDEAVFEEMRPYFCDLYGNPSSMHYFGGQVQRKVDEARNRVAALLGALPEEIIFTACGTESDNAAIR
SALEVFPERRHIITTRVEHPAVLTLCRNLSKRGYRVTELGVDGEGRLDLNELRSAIDEDTVVVSVMWANNETGVIFPVEE
ISRIVKEKGKGALFHTDAVQAVGKIPINMATSSIDMLSISGHKLHAPKGTGVLYLRKGVPFRPFMVGGHQEHSRRAGTEN
TAGIIALGKACELAGHWMEDENTRVKALRDRLEAALLELIPRARINGGEAERLPNTLSIAFEFVEGEAILMLMSEKGICA
SSGSACTSGSLEPSHVLRAMGVPFTCAHGSIRFSLSRYTTDEEIDTIIRELPPIIRRLREMSPFGREFLNA
>Mature_391_residues
MKEIYLDNNATTKVDEAVFEEMRPYFCDLYGNPSSMHYFGGQVQRKVDEARNRVAALLGALPEEIIFTACGTESDNAAIR
SALEVFPERRHIITTRVEHPAVLTLCRNLSKRGYRVTELGVDGEGRLDLNELRSAIDEDTVVVSVMWANNETGVIFPVEE
ISRIVKEKGKGALFHTDAVQAVGKIPINMATSSIDMLSISGHKLHAPKGTGVLYLRKGVPFRPFMVGGHQEHSRRAGTEN
TAGIIALGKACELAGHWMEDENTRVKALRDRLEAALLELIPRARINGGEAERLPNTLSIAFEFVEGEAILMLMSEKGICA
SSGSACTSGSLEPSHVLRAMGVPFTCAHGSIRFSLSRYTTDEEIDTIIRELPPIIRRLREMSPFGREFLNA

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=388, Percent_Identity=44.3298969072165, Blast_Score=327, Evalue=8e-90,
Organism=Homo sapiens, GI156713448, Length=417, Percent_Identity=32.6139088729017, Blast_Score=206, Evalue=4e-53,
Organism=Escherichia coli, GI48994898, Length=387, Percent_Identity=43.9276485788114, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1789175, Length=389, Percent_Identity=25.9640102827763, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787970, Length=400, Percent_Identity=25.75, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=392, Percent_Identity=42.3469387755102, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17533177, Length=318, Percent_Identity=34.5911949685535, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6319831, Length=387, Percent_Identity=42.1188630490956, Blast_Score=298, Evalue=8e-82,
Organism=Drosophila melanogaster, GI20129463, Length=387, Percent_Identity=42.8940568475452, Blast_Score=305, Evalue=5e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43154; Mature: 43154

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIYLDNNATTKVDEAVFEEMRPYFCDLYGNPSSMHYFGGQVQRKVDEARNRVAALLGA
CCEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHHC
LPEEIIFTACGTESDNAAIRSALEVFPERRHIITTRVEHPAVLTLCRNLSKRGYRVTELG
CCHHHEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEC
VDGEGRLDLNELRSAIDEDTVVVSVMWANNETGVIFPVEEISRIVKEKGKGALFHTDAVQ
CCCCCCCCHHHHHHHCCCCEEEEEEEEECCCCCEEEEHHHHHHHHHHCCCCCEEEHHHHH
AVGKIPINMATSSIDMLSISGHKLHAPKGTGVLYLRKGVPFRPFMVGGHQEHSRRAGTEN
HHHCCCEEEECCCEEEEEECCCEEECCCCCCEEEEECCCCCCCEEECCCHHHHHHCCCCC
TAGIIALGKACELAGHWMEDENTRVKALRDRLEAALLELIPRARINGGEAERLPNTLSIA
CCCEEEECHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEE
FEFVEGEAILMLMSEKGICASSGSACTSGSLEPSHVLRAMGVPFTCAHGSIRFSLSRYTT
EEEECCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCCEEEEEECCCC
DEEIDTIIRELPPIIRRLREMSPFGREFLNA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MKEIYLDNNATTKVDEAVFEEMRPYFCDLYGNPSSMHYFGGQVQRKVDEARNRVAALLGA
CCEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHHC
LPEEIIFTACGTESDNAAIRSALEVFPERRHIITTRVEHPAVLTLCRNLSKRGYRVTELG
CCHHHEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEC
VDGEGRLDLNELRSAIDEDTVVVSVMWANNETGVIFPVEEISRIVKEKGKGALFHTDAVQ
CCCCCCCCHHHHHHHCCCCEEEEEEEEECCCCCEEEEHHHHHHHHHHCCCCCEEEHHHHH
AVGKIPINMATSSIDMLSISGHKLHAPKGTGVLYLRKGVPFRPFMVGGHQEHSRRAGTEN
HHHCCCEEEECCCEEEEEECCCEEECCCCCCEEEEECCCCCCCEEECCCHHHHHHCCCCC
TAGIIALGKACELAGHWMEDENTRVKALRDRLEAALLELIPRARINGGEAERLPNTLSIA
CCCEEEECHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEE
FEFVEGEAILMLMSEKGICASSGSACTSGSLEPSHVLRAMGVPFTCAHGSIRFSLSRYTT
EEEECCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCCEEEEEECCCC
DEEIDTIIRELPPIIRRLREMSPFGREFLNA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]