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The map label for this gene is pfk [H]

Identifier: 39996803

GI number: 39996803

Start: 1865397

End: 1866488

Strand: Direct

Name: pfk [H]

Synonym: GSU1703

Alternate gene names: 39996803

Gene position: 1865397-1866488 (Clockwise)

Preceding gene: 39996802

Following gene: 39996804

Centisome position: 48.91

GC content: 61.08

Gene sequence:

>1092_bases
ATGGCGCGTAAAGTAGGCATACTGACCGGGGGGGGAGACTGCCCGGGACTCAATGCGGTGATCAGGGGAGTTGTCAAAAG
CTCCATCATCCGGCGCGGCTGGGAAGTGGTCGGCATCAGGGACGGGTTCGACGGCCTGCTCTACAACAACAGAATCGTAC
CGCTCGGTCTCAATGAAGTACGTGGAATACTCCCCCGCGGCGGAACCATCCTCGGTACGTCCAATCGCGGCAATCCCTTC
TCATACCCTGTTGAAGCGGATGGCAAAACCGTTTTAACCGATGTGTCCGACGAGGTCGTCGCGAACATCAAAAAACAGGG
AATCGACGCGCTCGTGGCAGTGGGAGGGGACGGCTCGCTCAAGATCGCGCTTGAGCTCATGAACAAGGGGATTCCCGTTG
TGGGCGTGCCGAAGACCATTGACAACGATCTGATGGAGACCGATGTCACCTTTGGCTACAACACCGCCCTGGAAACCGCA
ACCGATGCCCTGGACAAGCTTCACTCCACGGCCGAGAGTCACCACCGGGTCATGATCATGGAGGTCATGGGGCGCTATGC
CGGTTGGATCGCTCTCGAATCGGGCATCAGCGGCGGTGCGGACGTCATTCTCATCCCCGAGATTCCCTACGATATCAGTG
CCGTCTGCCGCGCGGTCGACGAGCGGCGAAGACGGGGAAGCTCCTTCAGTATCATCGTGGTGGCGGAGGGGGCGTTCCCG
CGCGGCGGCAACCGGGTCGTGCAGAAGCGGGCCGATGAAACGAACACCATCGAGCGGCTTGGGGGGATCGGTCAATACGT
GGCCCGGCAGCTGGGAGACTGCCTCGACATGGATGTTCGCGTCATGGTCCTGGGCCACCTTCAACGGGGCGGTTCACCAT
CGACCTTTGACCGTTGCCTAGGCAGCCGCTTCGGCGTTGCCGCAATAGACCTCATTGAACAGGAACAGTATGGACGCATG
GTCTGTCTGCGCGGCCGCGACATCAAATCGGTATCCATCGAGCGGGCGGTGCGGAAGCTGAAACTGGTGAACCCCGGCGG
TCAGATGGTGACGGCGGCGGAAGAGCTCGGCATCGTGGTGGGGCGGCGGTGA

Upstream 100 bases:

>100_bases
CGACGGAGGACTCCTGGGAAGCGCTGCGCTGGCCCGTGATGAGTTCGACATCTAAGAGAGTAGAGAGTATTTTCATAGTT
ATCAACGACGGGGAGGAATC

Downstream 100 bases:

>100_bases
GAGTTGACATGGAATGGCCCACGAGCTAGAGTGCTCTCGTTTTTTCTTAAGAAATTCTTTCCGCCGCCGATGTTATACAT
AGTGCATATGAGCCGAACTG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 2; Phosphohexokinase 2 [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MARKVGILTGGGDCPGLNAVIRGVVKSSIIRRGWEVVGIRDGFDGLLYNNRIVPLGLNEVRGILPRGGTILGTSNRGNPF
SYPVEADGKTVLTDVSDEVVANIKKQGIDALVAVGGDGSLKIALELMNKGIPVVGVPKTIDNDLMETDVTFGYNTALETA
TDALDKLHSTAESHHRVMIMEVMGRYAGWIALESGISGGADVILIPEIPYDISAVCRAVDERRRRGSSFSIIVVAEGAFP
RGGNRVVQKRADETNTIERLGGIGQYVARQLGDCLDMDVRVMVLGHLQRGGSPSTFDRCLGSRFGVAAIDLIEQEQYGRM
VCLRGRDIKSVSIERAVRKLKLVNPGGQMVTAAEELGIVVGRR

Sequences:

>Translated_363_residues
MARKVGILTGGGDCPGLNAVIRGVVKSSIIRRGWEVVGIRDGFDGLLYNNRIVPLGLNEVRGILPRGGTILGTSNRGNPF
SYPVEADGKTVLTDVSDEVVANIKKQGIDALVAVGGDGSLKIALELMNKGIPVVGVPKTIDNDLMETDVTFGYNTALETA
TDALDKLHSTAESHHRVMIMEVMGRYAGWIALESGISGGADVILIPEIPYDISAVCRAVDERRRRGSSFSIIVVAEGAFP
RGGNRVVQKRADETNTIERLGGIGQYVARQLGDCLDMDVRVMVLGHLQRGGSPSTFDRCLGSRFGVAAIDLIEQEQYGRM
VCLRGRDIKSVSIERAVRKLKLVNPGGQMVTAAEELGIVVGRR
>Mature_362_residues
ARKVGILTGGGDCPGLNAVIRGVVKSSIIRRGWEVVGIRDGFDGLLYNNRIVPLGLNEVRGILPRGGTILGTSNRGNPFS
YPVEADGKTVLTDVSDEVVANIKKQGIDALVAVGGDGSLKIALELMNKGIPVVGVPKTIDNDLMETDVTFGYNTALETAT
DALDKLHSTAESHHRVMIMEVMGRYAGWIALESGISGGADVILIPEIPYDISAVCRAVDERRRRGSSFSIIVVAEGAFPR
GGNRVVQKRADETNTIERLGGIGQYVARQLGDCLDMDVRVMVLGHLQRGGSPSTFDRCLGSRFGVAAIDLIEQEQYGRMV
CLRGRDIKSVSIERAVRKLKLVNPGGQMVTAAEELGIVVGRR

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453619, Length=336, Percent_Identity=36.0119047619048, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI266453768, Length=336, Percent_Identity=36.0119047619048, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI266453748, Length=336, Percent_Identity=36.0119047619048, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI4505749, Length=336, Percent_Identity=36.0119047619048, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI48762920, Length=334, Percent_Identity=33.8323353293413, Blast_Score=164, Evalue=9e-41,
Organism=Homo sapiens, GI11321601, Length=334, Percent_Identity=34.1317365269461, Blast_Score=162, Evalue=6e-40,
Organism=Escherichia coli, GI1790350, Length=341, Percent_Identity=36.950146627566, Blast_Score=211, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17558788, Length=379, Percent_Identity=31.9261213720317, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI25147584, Length=372, Percent_Identity=31.4516129032258, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI72003601, Length=319, Percent_Identity=28.8401253918495, Blast_Score=111, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6321679, Length=341, Percent_Identity=34.0175953079179, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6323861, Length=339, Percent_Identity=31.858407079646, Blast_Score=150, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24652337, Length=331, Percent_Identity=34.441087613293, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI17647809, Length=331, Percent_Identity=34.441087613293, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI28573326, Length=331, Percent_Identity=33.8368580060423, Blast_Score=163, Evalue=2e-40,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 38937; Mature: 38805

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKVGILTGGGDCPGLNAVIRGVVKSSIIRRGWEVVGIRDGFDGLLYNNRIVPLGLNEV
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEECCEEEECCHHHH
RGILPRGGTILGTSNRGNPFSYPVEADGKTVLTDVSDEVVANIKKQGIDALVAVGGDGSL
HHCCCCCCEEEECCCCCCCEECCCCCCCCEEEECCCHHHHHHHHHCCCEEEEEECCCCCE
KIALELMNKGIPVVGVPKTIDNDLMETDVTFGYNTALETATDALDKLHSTAESHHRVMIM
EEEEEHHHCCCCEEECCCHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
EVMGRYAGWIALESGISGGADVILIPEIPYDISAVCRAVDERRRRGSSFSIIVVAEGAFP
HHHHHHHCEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCC
RGGNRVVQKRADETNTIERLGGIGQYVARQLGDCLDMDVRVMVLGHLQRGGSPSTFDRCL
CCCCHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHCCCHHEEEEEHHHCCCCCCHHHHHH
GSRFGVAAIDLIEQEQYGRMVCLRGRDIKSVSIERAVRKLKLVNPGGQMVTAAEELGIVV
CCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHEEECCCCCEEEEHHHHCEEE
GRR
CCC
>Mature Secondary Structure 
ARKVGILTGGGDCPGLNAVIRGVVKSSIIRRGWEVVGIRDGFDGLLYNNRIVPLGLNEV
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEECCEEEECCHHHH
RGILPRGGTILGTSNRGNPFSYPVEADGKTVLTDVSDEVVANIKKQGIDALVAVGGDGSL
HHCCCCCCEEEECCCCCCCEECCCCCCCCEEEECCCHHHHHHHHHCCCEEEEEECCCCCE
KIALELMNKGIPVVGVPKTIDNDLMETDVTFGYNTALETATDALDKLHSTAESHHRVMIM
EEEEEHHHCCCCEEECCCHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
EVMGRYAGWIALESGISGGADVILIPEIPYDISAVCRAVDERRRRGSSFSIIVVAEGAFP
HHHHHHHCEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCC
RGGNRVVQKRADETNTIERLGGIGQYVARQLGDCLDMDVRVMVLGHLQRGGSPSTFDRCL
CCCCHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHCCCHHEEEEEHHHCCCCCCHHHHHH
GSRFGVAAIDLIEQEQYGRMVCLRGRDIKSVSIERAVRKLKLVNPGGQMVTAAEELGIVV
CCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHEEECCCCCEEEEHHHHCEEE
GRR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]