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The map label for this gene is glcD [H]

Identifier: 39996723

GI number: 39996723

Start: 1778047

End: 1779420

Strand: Direct

Name: glcD [H]

Synonym: GSU1623

Alternate gene names: 39996723

Gene position: 1778047-1779420 (Clockwise)

Preceding gene: 39996722

Following gene: 39996724

Centisome position: 46.62

GC content: 62.08

Gene sequence:

>1374_bases
ATGGACGTTTCATTCATTAGCGAACTGAAGAGCATAGTCGGCGAGGAACACACCCTCGCCGACAAGGAAAGCCTCGCCTG
TTACGGCTATGATTCAACGCCGGAGTATGAAAGCCGGCCCGGAGCGGTGGTCCTTCCCGCCAACGAGGAGGAGATCTGCC
GGATCATGGCCCGCTGCCATGCCGCAGGGGTCCCCGTGACTCCTCGCGGCTCCGGAACCAACCTGTCCGGAGGCTCTCTG
GGCCGCCCCGAAGGGGTTGTCGTTCAGACGAGCCGGCTGAACAGCATCGTTGAAATCGACGAGGAAAACCTGACCGCCAC
TGTCCAGCCCGGCGTCGTAACCAGCGCACTGCACCGGGCAGTGGAAGCAAAGGGGCTCTTCTATCCGCCCGATCCGGGCA
GCATGAACATCTCCACCATCGGCGGCAACGTGGCCGAAAACGCCGGTGGCTTGCGGGGCCTCAAATACGGAGTCACTGCC
GACTATGTCATGGGGCTCCACACCGCTCTACCCGACGGCAGCCTCCTGCGGACCGGCGGCAAAGCCGTAAAGGACGTTGC
CGGCTATAACCTGAACCAGCTCCTCGTCTCTTCCGAGGGGACCCTCGGGCTCTTCACCGAAGTGACTGTTAAACTGATTC
CCAAGCCCCAAGCCAAGAAGACGATGCTCGTCCATTTCCCGGTGCTGGAAAACGCGGCCCTGGCAGTCTCCCATATCGTG
GCCGCCCGGGTCATTCCGGCCACACTTGAGTTCATGGACCGGGTCACGGTCAAGTGTATCGAGGATTACGCCAAGGTGGG
CCTGCCCCTGGATGTGGAGGCCGTGCTCCTCATCGAAGTGGACGGTCATCCGGCCCAGGTGGAGGAAGACGCAGTCGCGG
TCCGGCGGATCTGCGCCGAGCACCATTGTTCGTCGTTCCAGACCGCGGCCGACGCCGACGAGGCTCTCAAGCTCGCTACT
GCCCGGCGGGTGGCCCTTTCGTCCCTGGCCCGGGTCAAGCCCACCACTATCCTTGAGGACGCCACTGTCCCCCGGAGCTG
CATTGCCCCCATGGTCAAGTTTATCCAGGACACGGCCCGGAAGTACAATCTCACCATCGGCACCTTCGGCCATGCCGGCG
ACGGCAACCTTCATCCCACTTGCCTCACCGACGAGCGGGATAAGGATGAGATCGCCCGGGCTCACAAGGCGTTCGAGGAA
ATCTTTGATACGGCTCTCGCCATGGGGGGAACTATCACCGGCGAGCATGGCGTGGGGCTTTCCAAGAAAAAGTATCTCCC
GCGGCTTGTGGGAGAGTCCGGGATACGGGTGATGCAGGGGATCAAGCAGGCATTCGATCCGACGGGAATCCTGAATCCGG
GGAAGGTGTTCTAG

Upstream 100 bases:

>100_bases
ACCCTAGCGTCCCCTGTCGGTAGCCCTGCGAAGGGAGTTGGGACTAACCGCACGGCACACGGGCTTCACCGTTGGGTAAC
CTTGCACCGGGAGAGCAGAT

Downstream 100 bases:

>100_bases
GGAGTATTCGATGGAATACGTAAAATTGATCAACTGCATGCGCTGCGGCATGTGTCTGCCCCAGTGCCCCACATACAAGG
AGACATTTCTGGAAACCGCC

Product: glycolate oxidase subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MDVSFISELKSIVGEEHTLADKESLACYGYDSTPEYESRPGAVVLPANEEEICRIMARCHAAGVPVTPRGSGTNLSGGSL
GRPEGVVVQTSRLNSIVEIDEENLTATVQPGVVTSALHRAVEAKGLFYPPDPGSMNISTIGGNVAENAGGLRGLKYGVTA
DYVMGLHTALPDGSLLRTGGKAVKDVAGYNLNQLLVSSEGTLGLFTEVTVKLIPKPQAKKTMLVHFPVLENAALAVSHIV
AARVIPATLEFMDRVTVKCIEDYAKVGLPLDVEAVLLIEVDGHPAQVEEDAVAVRRICAEHHCSSFQTAADADEALKLAT
ARRVALSSLARVKPTTILEDATVPRSCIAPMVKFIQDTARKYNLTIGTFGHAGDGNLHPTCLTDERDKDEIARAHKAFEE
IFDTALAMGGTITGEHGVGLSKKKYLPRLVGESGIRVMQGIKQAFDPTGILNPGKVF

Sequences:

>Translated_457_residues
MDVSFISELKSIVGEEHTLADKESLACYGYDSTPEYESRPGAVVLPANEEEICRIMARCHAAGVPVTPRGSGTNLSGGSL
GRPEGVVVQTSRLNSIVEIDEENLTATVQPGVVTSALHRAVEAKGLFYPPDPGSMNISTIGGNVAENAGGLRGLKYGVTA
DYVMGLHTALPDGSLLRTGGKAVKDVAGYNLNQLLVSSEGTLGLFTEVTVKLIPKPQAKKTMLVHFPVLENAALAVSHIV
AARVIPATLEFMDRVTVKCIEDYAKVGLPLDVEAVLLIEVDGHPAQVEEDAVAVRRICAEHHCSSFQTAADADEALKLAT
ARRVALSSLARVKPTTILEDATVPRSCIAPMVKFIQDTARKYNLTIGTFGHAGDGNLHPTCLTDERDKDEIARAHKAFEE
IFDTALAMGGTITGEHGVGLSKKKYLPRLVGESGIRVMQGIKQAFDPTGILNPGKVF
>Mature_457_residues
MDVSFISELKSIVGEEHTLADKESLACYGYDSTPEYESRPGAVVLPANEEEICRIMARCHAAGVPVTPRGSGTNLSGGSL
GRPEGVVVQTSRLNSIVEIDEENLTATVQPGVVTSALHRAVEAKGLFYPPDPGSMNISTIGGNVAENAGGLRGLKYGVTA
DYVMGLHTALPDGSLLRTGGKAVKDVAGYNLNQLLVSSEGTLGLFTEVTVKLIPKPQAKKTMLVHFPVLENAALAVSHIV
AARVIPATLEFMDRVTVKCIEDYAKVGLPLDVEAVLLIEVDGHPAQVEEDAVAVRRICAEHHCSSFQTAADADEALKLAT
ARRVALSSLARVKPTTILEDATVPRSCIAPMVKFIQDTARKYNLTIGTFGHAGDGNLHPTCLTDERDKDEIARAHKAFEE
IFDTALAMGGTITGEHGVGLSKKKYLPRLVGESGIRVMQGIKQAFDPTGILNPGKVF

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=458, Percent_Identity=35.3711790393013, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI37595754, Length=481, Percent_Identity=33.6798336798337, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI119964728, Length=446, Percent_Identity=28.47533632287, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI4501993, Length=222, Percent_Identity=29.2792792792793, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1789351, Length=452, Percent_Identity=38.2743362831858, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1787977, Length=504, Percent_Identity=24.4047619047619, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI48994907, Length=500, Percent_Identity=23.6, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI48994914, Length=178, Percent_Identity=32.5842696629214, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71992373, Length=458, Percent_Identity=33.1877729257642, Blast_Score=248, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI71992381, Length=458, Percent_Identity=31.8777292576419, Blast_Score=229, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI71992392, Length=379, Percent_Identity=34.3007915567282, Blast_Score=211, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI17534361, Length=447, Percent_Identity=27.0693512304251, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17556096, Length=219, Percent_Identity=32.4200913242009, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320027, Length=435, Percent_Identity=33.5632183908046, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6320764, Length=495, Percent_Identity=27.0707070707071, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6320023, Length=471, Percent_Identity=27.1762208067941, Blast_Score=172, Evalue=9e-44,
Organism=Drosophila melanogaster, GI18921117, Length=460, Percent_Identity=25.4347826086956, Blast_Score=142, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24639277, Length=460, Percent_Identity=25.4347826086956, Blast_Score=142, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24639275, Length=460, Percent_Identity=25.4347826086956, Blast_Score=142, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24653753, Length=242, Percent_Identity=30.1652892561983, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 48628; Mature: 48628

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVSFISELKSIVGEEHTLADKESLACYGYDSTPEYESRPGAVVLPANEEEICRIMARCH
CCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEECCCHHHHHHHHHHHH
AAGVPVTPRGSGTNLSGGSLGRPEGVVVQTSRLNSIVEIDEENLTATVQPGVVTSALHRA
HCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHCCCEEEECCCCCEEEECCCHHHHHHHHH
VEAKGLFYPPDPGSMNISTIGGNVAENAGGLRGLKYGVTADYVMGLHTALPDGSLLRTGG
HHHCCCEECCCCCCEEEEECCCCHHHCCCCCCCEECCCCHHHHHHHHHCCCCCCEECCCC
KAVKDVAGYNLNQLLVSSEGTLGLFTEVTVKLIPKPQAKKTMLVHFPVLENAALAVSHIV
HHHHHHHCCCHHHEEEECCCCEEEHEEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHH
AARVIPATLEFMDRVTVKCIEDYAKVGLPLDVEAVLLIEVDGHPAQVEEDAVAVRRICAE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHH
HHCSSFQTAADADEALKLATARRVALSSLARVKPTTILEDATVPRSCIAPMVKFIQDTAR
HHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHH
KYNLTIGTFGHAGDGNLHPTCLTDERDKDEIARAHKAFEEIFDTALAMGGTITGEHGVGL
HEEEEEEECCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SKKKYLPRLVGESGIRVMQGIKQAFDPTGILNPGKVF
CHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MDVSFISELKSIVGEEHTLADKESLACYGYDSTPEYESRPGAVVLPANEEEICRIMARCH
CCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEECCCHHHHHHHHHHHH
AAGVPVTPRGSGTNLSGGSLGRPEGVVVQTSRLNSIVEIDEENLTATVQPGVVTSALHRA
HCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHCCCEEEECCCCCEEEECCCHHHHHHHHH
VEAKGLFYPPDPGSMNISTIGGNVAENAGGLRGLKYGVTADYVMGLHTALPDGSLLRTGG
HHHCCCEECCCCCCEEEEECCCCHHHCCCCCCCEECCCCHHHHHHHHHCCCCCCEECCCC
KAVKDVAGYNLNQLLVSSEGTLGLFTEVTVKLIPKPQAKKTMLVHFPVLENAALAVSHIV
HHHHHHHCCCHHHEEEECCCCEEEHEEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHH
AARVIPATLEFMDRVTVKCIEDYAKVGLPLDVEAVLLIEVDGHPAQVEEDAVAVRRICAE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHH
HHCSSFQTAADADEALKLATARRVALSSLARVKPTTILEDATVPRSCIAPMVKFIQDTAR
HHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHH
KYNLTIGTFGHAGDGNLHPTCLTDERDKDEIARAHKAFEEIFDTALAMGGTITGEHGVGL
HEEEEEEECCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SKKKYLPRLVGESGIRVMQGIKQAFDPTGILNPGKVF
CHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]