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The map label for this gene is glcF [H]

Identifier: 39996724

GI number: 39996724

Start: 1779431

End: 1780681

Strand: Direct

Name: glcF [H]

Synonym: GSU1624

Alternate gene names: 39996724

Gene position: 1779431-1780681 (Clockwise)

Preceding gene: 39996723

Following gene: 39996726

Centisome position: 46.65

GC content: 61.63

Gene sequence:

>1251_bases
ATGGAATACGTAAAATTGATCAACTGCATGCGCTGCGGCATGTGTCTGCCCCAGTGCCCCACATACAAGGAGACATTTCT
GGAAACCGCCTCTCCCCGGGGGCGGGTGGCGCTCATGCGCAAGATCGAGGAGGGCGATCTCGAGGTGAGCGAGCGGCTGC
TGGAATACCTCTCGCTGTGTCTCGACTGTCAGGCATGCGCCTCGGCCTGCCCCTGTGGGGTAAATGCCGGCGAGATGGTG
GCTGGCTACAGGTGCGAACGCAGGGACGGGAAAGAGCTGTCGACGATGGAAAACCTCATCCTCAGAAGGCTTTTGCCGCA
TCCCGACCGGCTTGAGGCCGCAACGGCCCCCATGCGCCTCTATCAGCGGACAGGCCTTCAGAAGGTTGTCCGCAAACTCG
GCATCCTAAAGATGTTTCCCCCAACCCTTGAGCGGATGGAAGGACTCCTGCCGGAGCTTCCGGTCAAGCCCCTGCGCCAG
ACCATCGACGAGGTGACCCCGGCGGTGGGACCGGAGCGCGGAACAATCGGCTTTTTTCTCGGCTGTGTCATGAGCCTGGT
CTTTTCCGAGGCAAGCTATGCAACGGTGAAGCTCCTCTCATCGCTTGGCTATCGCGTCGTCACGCCGAAGGCCCAAAAGT
GCTGCGGCGCCCCCAACATGCTGTCGGGCGATCGGGAGGGGCTTCTGGATGCGGCGCGGTTCAATGTGGATCTCTTTGCC
GGCCATAGGCTTGATTTCATTGTCACCGACTGCGGTGGTTGCGGAGCCGAGCTCAAGAAATACGGCCATCACCTGGAAGA
TGACAGCCGGGCCGCGACCTTCAGTGCCCAGGTGCGCGACATCACGCAGGTCCTGGCCCTTCACGCCGACGAGCTGAAGG
CGAAGCTTAACCCTCTGTCGCTCAAGGTGACTTACCACGATCCCTGTCACATCGCCCACTGTCAGGGGATACGCCAGGAG
CCCCGCACGCTGCTCAGGATCGTGCCGGAGCTCGATTTCCGCGAGCTTCCCGAGGCCGATGCCTGCTGCGGCAGTGGTGG
TACGTACAACATCGAGAAGCCGGAGATGTCCGACCGCGTGCTGCGCCGCAAGGTGGAGAACATCAAGGCCACTGGGGCCG
ACGTTCTCGTCACTGCCAACCCCGGCTGTCTTCTCCAGCTTCGTAAGGCCTTGGCCCAGGAGACGCCGCCCATCCGGGTA
CTGCATGTCACTGAGGTGCTTGCAGCAAGCCTCTTGCCCCCATTACTCTGA

Upstream 100 bases:

>100_bases
CTTGTGGGAGAGTCCGGGATACGGGTGATGCAGGGGATCAAGCAGGCATTCGATCCGACGGGAATCCTGAATCCGGGGAA
GGTGTTCTAGGGAGTATTCG

Downstream 100 bases:

>100_bases
ACAGTCACCAGCCGATTCGGGGGCAGTTGTTCGCCCCCGGATGTCCTGCTACACCAGTACGTTTGAATGCTCCTCTGACC
GCTTGCTCAGGATAATACTG

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MEYVKLINCMRCGMCLPQCPTYKETFLETASPRGRVALMRKIEEGDLEVSERLLEYLSLCLDCQACASACPCGVNAGEMV
AGYRCERRDGKELSTMENLILRRLLPHPDRLEAATAPMRLYQRTGLQKVVRKLGILKMFPPTLERMEGLLPELPVKPLRQ
TIDEVTPAVGPERGTIGFFLGCVMSLVFSEASYATVKLLSSLGYRVVTPKAQKCCGAPNMLSGDREGLLDAARFNVDLFA
GHRLDFIVTDCGGCGAELKKYGHHLEDDSRAATFSAQVRDITQVLALHADELKAKLNPLSLKVTYHDPCHIAHCQGIRQE
PRTLLRIVPELDFRELPEADACCGSGGTYNIEKPEMSDRVLRRKVENIKATGADVLVTANPGCLLQLRKALAQETPPIRV
LHVTEVLAASLLPPLL

Sequences:

>Translated_416_residues
MEYVKLINCMRCGMCLPQCPTYKETFLETASPRGRVALMRKIEEGDLEVSERLLEYLSLCLDCQACASACPCGVNAGEMV
AGYRCERRDGKELSTMENLILRRLLPHPDRLEAATAPMRLYQRTGLQKVVRKLGILKMFPPTLERMEGLLPELPVKPLRQ
TIDEVTPAVGPERGTIGFFLGCVMSLVFSEASYATVKLLSSLGYRVVTPKAQKCCGAPNMLSGDREGLLDAARFNVDLFA
GHRLDFIVTDCGGCGAELKKYGHHLEDDSRAATFSAQVRDITQVLALHADELKAKLNPLSLKVTYHDPCHIAHCQGIRQE
PRTLLRIVPELDFRELPEADACCGSGGTYNIEKPEMSDRVLRRKVENIKATGADVLVTANPGCLLQLRKALAQETPPIRV
LHVTEVLAASLLPPLL
>Mature_416_residues
MEYVKLINCMRCGMCLPQCPTYKETFLETASPRGRVALMRKIEEGDLEVSERLLEYLSLCLDCQACASACPCGVNAGEMV
AGYRCERRDGKELSTMENLILRRLLPHPDRLEAATAPMRLYQRTGLQKVVRKLGILKMFPPTLERMEGLLPELPVKPLRQ
TIDEVTPAVGPERGTIGFFLGCVMSLVFSEASYATVKLLSSLGYRVVTPKAQKCCGAPNMLSGDREGLLDAARFNVDLFA
GHRLDFIVTDCGGCGAELKKYGHHLEDDSRAATFSAQVRDITQVLALHADELKAKLNPLSLKVTYHDPCHIAHCQGIRQE
PRTLLRIVPELDFRELPEADACCGSGGTYNIEKPEMSDRVLRRKVENIKATGADVLVTANPGCLLQLRKALAQETPPIRV
LHVTEVLAASLLPPLL

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=395, Percent_Identity=27.8481012658228, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1786497, Length=239, Percent_Identity=30.9623430962343, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1788576, Length=425, Percent_Identity=24.9411764705882, Blast_Score=95, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 45943; Mature: 45943

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
4.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYVKLINCMRCGMCLPQCPTYKETFLETASPRGRVALMRKIEEGDLEVSERLLEYLSLC
CCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
LDCQACASACPCGVNAGEMVAGYRCERRDGKELSTMENLILRRLLPHPDRLEAATAPMRL
HHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
YQRTGLQKVVRKLGILKMFPPTLERMEGLLPELPVKPLRQTIDEVTPAVGPERGTIGFFL
HHHCCHHHHHHHHCCHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHH
GCVMSLVFSEASYATVKLLSSLGYRVVTPKAQKCCGAPNMLSGDREGLLDAARFNVDLFA
HHHHHHHHCCCHHHHHHHHHHCCCEEECCCHHHHCCCCCCCCCCHHHHHHHHHHCEEEEE
GHRLDFIVTDCGGCGAELKKYGHHLEDDSRAATFSAQVRDITQVLALHADELKAKLNPLS
CCCEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LKVTYHDPCHIAHCQGIRQEPRTLLRIVPELDFRELPEADACCGSGGTYNIEKPEMSDRV
EEEEECCCCCHHHHHCCCCCHHHHHHHHCCCCHHHCCCHHHCCCCCCCCCCCCCCHHHHH
LRRKVENIKATGADVLVTANPGCLLQLRKALAQETPPIRVLHVTEVLAASLLPPLL
HHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEYVKLINCMRCGMCLPQCPTYKETFLETASPRGRVALMRKIEEGDLEVSERLLEYLSLC
CCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
LDCQACASACPCGVNAGEMVAGYRCERRDGKELSTMENLILRRLLPHPDRLEAATAPMRL
HHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
YQRTGLQKVVRKLGILKMFPPTLERMEGLLPELPVKPLRQTIDEVTPAVGPERGTIGFFL
HHHCCHHHHHHHHCCHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHH
GCVMSLVFSEASYATVKLLSSLGYRVVTPKAQKCCGAPNMLSGDREGLLDAARFNVDLFA
HHHHHHHHCCCHHHHHHHHHHCCCEEECCCHHHHCCCCCCCCCCHHHHHHHHHHCEEEEE
GHRLDFIVTDCGGCGAELKKYGHHLEDDSRAATFSAQVRDITQVLALHADELKAKLNPLS
CCCEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LKVTYHDPCHIAHCQGIRQEPRTLLRIVPELDFRELPEADACCGSGGTYNIEKPEMSDRV
EEEEECCCCCHHHHHCCCCCHHHHHHHHCCCCHHHCCCHHHCCCCCCCCCCCCCCHHHHH
LRRKVENIKATGADVLVTANPGCLLQLRKALAQETPPIRVLHVTEVLAASLLPPLL
HHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]