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The map label for this gene is pilT-4 [H]

Identifier: 39996592

GI number: 39996592

Start: 1636677

End: 1637774

Strand: Direct

Name: pilT-4 [H]

Synonym: GSU1492

Alternate gene names: 39996592

Gene position: 1636677-1637774 (Clockwise)

Preceding gene: 39996591

Following gene: 39996593

Centisome position: 42.91

GC content: 59.84

Gene sequence:

>1098_bases
ATGGCCAACATGCATCAACTCCTCACGGAACTCGTCAATCGCGGGGGCTCGGACCTTCACATCACCACTAACTCGCCCCC
GCAGATCCGGGTCGACGGCCAGTTGATCCCTCTCGAGATGCCACCGCTCAATGCGGTGGACACCAAGCAGCTCTGCTACA
GCATCCTTACTGAGCAGCAGAAGCACAAATTCGAGGAAGCCAACGAACTCGACCTTTCCTTCGGCATCAAGGGGCTTTCC
CGTTTCCGGGGGAACGTGTTCATTCAGCGGGGCGCAGTTGCCGGCGTGTTCCGGGTGATTCCCTACAAGATTCTCACCTT
TGAAGAGCTGGGTCTGCCGGTGGTGGTGAAAGAGCTGGCGGAGAAGCCTCGGGGGCTGATCCTCGTTACCGGACCCACCG
GCAGCGGCAAGTCGACCACCCTGGCCGCCATCATCGACAAGATCAACACCGAACGGCACGATCATATTGTGACCATCGAG
GATCCCATCGAGTACCTGCATCCTCACAAGAGTTGCGTGGTGAACCAGCGTGAGGTGGGGGCCGATACGAAAAGCTTCAA
GAACGCCTTGAAATATATCCTGCGCCAGGATCCGGACGTGGTCCTCGTGGGGGAGCTCCGGGATCTGGAGACCATCGAGG
CGGCCCTGACCCTTGCCGAGACCGGTCACCTCTGTCTCGCCACGCTCCACACAAACTCGGCGGTGCAGACCATCAACCGG
ATCGTGGACGTATTTCCCCCCTACCAGCAGCCCCAGGTCCGTGCCCAGCTTTCTTTCGTTCTGGAAGGGGTCATGTCCCA
GACCCTGCTGCCGAACGTATCGGGCAAGGGGAGGGTGCTGGCCCTGGAGGTGATGGTGCCCAATCCGGCCATCCGTAACC
TGATCCGCGAAGACAAGATCCACCAGATCTATTCCCAGATGCAGGTGGGGCAGGAGAAGTTCGGCATGCAGACCATGAAC
CAGTCGCTGTTCAGTCTGTTGCAGAAGAGACGGATTTCGCTTGATGTGGCCATGGCCCGCTCGTCCGATCCCGACGAACT
CAAGCAGATGCTGGCCAGCGCCCAGCGGCCACCGGGTCAGCGCCCTCAGATGAGGTAA

Upstream 100 bases:

>100_bases
GCGTGGCTCTATCGCGGCTTCCTCAGCGTACTTCCCATAAGCCCCGGAGCCTCAATCCCGCTCATCTGAGATCTGACATT
TTGTAACGGAGAACATCATA

Downstream 100 bases:

>100_bases
CCGCCGCCGGATCGATCTGTACACAGGAGGAGAAAGATGCCCAAGTTCAACTGGGAAGCAAGAAGCAGGACCGGTTCCGT
TCAGAAGGGGGTCATGGAGG

Product: twitching motility protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MANMHQLLTELVNRGGSDLHITTNSPPQIRVDGQLIPLEMPPLNAVDTKQLCYSILTEQQKHKFEEANELDLSFGIKGLS
RFRGNVFIQRGAVAGVFRVIPYKILTFEELGLPVVVKELAEKPRGLILVTGPTGSGKSTTLAAIIDKINTERHDHIVTIE
DPIEYLHPHKSCVVNQREVGADTKSFKNALKYILRQDPDVVLVGELRDLETIEAALTLAETGHLCLATLHTNSAVQTINR
IVDVFPPYQQPQVRAQLSFVLEGVMSQTLLPNVSGKGRVLALEVMVPNPAIRNLIREDKIHQIYSQMQVGQEKFGMQTMN
QSLFSLLQKRRISLDVAMARSSDPDELKQMLASAQRPPGQRPQMR

Sequences:

>Translated_365_residues
MANMHQLLTELVNRGGSDLHITTNSPPQIRVDGQLIPLEMPPLNAVDTKQLCYSILTEQQKHKFEEANELDLSFGIKGLS
RFRGNVFIQRGAVAGVFRVIPYKILTFEELGLPVVVKELAEKPRGLILVTGPTGSGKSTTLAAIIDKINTERHDHIVTIE
DPIEYLHPHKSCVVNQREVGADTKSFKNALKYILRQDPDVVLVGELRDLETIEAALTLAETGHLCLATLHTNSAVQTINR
IVDVFPPYQQPQVRAQLSFVLEGVMSQTLLPNVSGKGRVLALEVMVPNPAIRNLIREDKIHQIYSQMQVGQEKFGMQTMN
QSLFSLLQKRRISLDVAMARSSDPDELKQMLASAQRPPGQRPQMR
>Mature_364_residues
ANMHQLLTELVNRGGSDLHITTNSPPQIRVDGQLIPLEMPPLNAVDTKQLCYSILTEQQKHKFEEANELDLSFGIKGLSR
FRGNVFIQRGAVAGVFRVIPYKILTFEELGLPVVVKELAEKPRGLILVTGPTGSGKSTTLAAIIDKINTERHDHIVTIED
PIEYLHPHKSCVVNQREVGADTKSFKNALKYILRQDPDVVLVGELRDLETIEAALTLAETGHLCLATLHTNSAVQTINRI
VDVFPPYQQPQVRAQLSFVLEGVMSQTLLPNVSGKGRVLALEVMVPNPAIRNLIREDKIHQIYSQMQVGQEKFGMQTMNQ
SLFSLLQKRRISLDVAMARSSDPDELKQMLASAQRPPGQRPQMR

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=320, Percent_Identity=42.8125, Blast_Score=256, Evalue=1e-69,
Organism=Escherichia coli, GI1789723, Length=253, Percent_Identity=35.5731225296443, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1786296, Length=286, Percent_Identity=33.2167832167832, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 40714; Mature: 40583

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANMHQLLTELVNRGGSDLHITTNSPPQIRVDGQLIPLEMPPLNAVDTKQLCYSILTEQQ
CCCHHHHHHHHHHCCCCEEEEECCCCCEEEECCEEEEECCCCCCCCCHHHHHHHHHHHHH
KHKFEEANELDLSFGIKGLSRFRGNVFIQRGAVAGVFRVIPYKILTFEELGLPVVVKELA
HHHHHCCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHEEHHHCCCHHHHHHHH
EKPRGLILVTGPTGSGKSTTLAAIIDKINTERHDHIVTIEDPIEYLHPHKSCVVNQREVG
HCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCHHHHCCCHHHCCCHHHCC
ADTKSFKNALKYILRQDPDVVLVGELRDLETIEAALTLAETGHLCLATLHTNSAVQTINR
CCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHH
IVDVFPPYQQPQVRAQLSFVLEGVMSQTLLPNVSGKGRVLALEVMVPNPAIRNLIREDKI
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHHHH
HQIYSQMQVGQEKFGMQTMNQSLFSLLQKRRISLDVAMARSSDPDELKQMLASAQRPPGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCC
RPQMR
CCCCC
>Mature Secondary Structure 
ANMHQLLTELVNRGGSDLHITTNSPPQIRVDGQLIPLEMPPLNAVDTKQLCYSILTEQQ
CCHHHHHHHHHHCCCCEEEEECCCCCEEEECCEEEEECCCCCCCCCHHHHHHHHHHHHH
KHKFEEANELDLSFGIKGLSRFRGNVFIQRGAVAGVFRVIPYKILTFEELGLPVVVKELA
HHHHHCCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHEEHHHCCCHHHHHHHH
EKPRGLILVTGPTGSGKSTTLAAIIDKINTERHDHIVTIEDPIEYLHPHKSCVVNQREVG
HCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCHHHHCCCHHHCCCHHHCC
ADTKSFKNALKYILRQDPDVVLVGELRDLETIEAALTLAETGHLCLATLHTNSAVQTINR
CCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHH
IVDVFPPYQQPQVRAQLSFVLEGVMSQTLLPNVSGKGRVLALEVMVPNPAIRNLIREDKI
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHHHH
HQIYSQMQVGQEKFGMQTMNQSLFSLLQKRRISLDVAMARSSDPDELKQMLASAQRPPGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCC
RPQMR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]