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The map label for this gene is tapB [H]

Identifier: 39996591

GI number: 39996591

Start: 1634843

End: 1636549

Strand: Direct

Name: tapB [H]

Synonym: GSU1491

Alternate gene names: 39996591

Gene position: 1634843-1636549 (Clockwise)

Preceding gene: 39996590

Following gene: 39996592

Centisome position: 42.86

GC content: 58.64

Gene sequence:

>1707_bases
ATGCAGGCTAGCAGACTGGGAGAACTCCTGGTTCGAAACAATATCATCACCAAGGAACAGCTTGCCAAGGCACTGGACGA
GCAACGAACCTCCGGCGGCCAACAGCGCCTTGGCTCCATCCTCGTCAAGAACGGCCTTGTCACTGAGCCCGATCTCACCA
CGTTCCTCTCCAAGCAGTACGGTGTCCCCTCCATCAATCTGAGTGAATTCGAAGCCGATATGGCGGTGGTCAAGATCATC
CCCGCCGACGTGGCCCAGAAGTACCAGATCGTCCCGGTGAACAGGGCCGGTTCGACCCTGATCATTGCCATGGCTGACCC
GTCGAACATCTTCGCCATCGACGACATCAAGTTCATGACCGGCTACAACGTGGAGGTGGTCGTCGCTTCCGAGTCGTCCA
TCAAGACTGCCATCGACAAGTACTATGACCAGTCAGCGTCTCTGGCCGACGTCATGAACGACCTGGAGATGGACGATCTG
GAGGTTATCGGCGAGGACGAGGATGTAGACGTTTCTTCCCTCGAACGGGCGACCGAGGATGCGCCGGTCGTCAAGCTCGT
GAACCTGATCCTCACCGACGCCATCAAGAAGAAGGCGAGTGATATCCATATCGAGCCCTATGAGCGGACCTTCCGGGTGC
GCTATCGGATCGACGGCGTCCTCTACGAAGTCATGAAGCCCCCCCTGAAACTGAAAAACGCCATCACGTCGCGGATCAAG
ATCATGGCTGACCTGGATATTGCCGAACGGCGGCTTCCCCAGGACGGCCGCATCAAGATCAAAATGGGTGGCGGGCAGGA
CATGGACTACCGGGTGTCGGTCCTGCCGACCCTGTTCGGCGAGAAGGTGGTTCTGCGACTCCTGGACAAGTCGAACCTCC
AGCTCGACATGACCAAGCTGGGCTACGAGCCCACGGCGTTGAGCTACTTCAAGGAGGCGATTCACAAGCCCTTCGGCATG
GTGCTGGTGACCGGACCCACGGGGAGCGGCAAGACGGTTTCCCTCTATTCGGCGCTTTCCGAGCTCAACAAGACTACCGA
GAACATCTCCACGGCCGAAGACCCGGTGGAGTTCAACTTCGCCGGCATCAACCAGGTGCAGATGCACGAAGATATCGGTC
TTACCTTTGCCGCCGCGCTCCGCTCCTTCCTGCGTCAGGACCCGGACATCATCATGATCGGAGAGATCCGGGACTTCGAG
ACGGCCGAGATCGCCATCAAGGCTGCGCTTACCGGTCACTTGGTTCTCTCTACCCTTCACACCAACGATGCCCCGGCCAC
CATCAACCGGCTGTTGAACATGGGGGTCGAGCCGTTTCTTGTTGCCTCGGCGGTGAACCTGATTACCGCCCAGCGTCTTG
CCCGGCGGGTCTGCTCCGAGTGCAAGGCCGTGGAGGAGATACCGATCCAGGCTTTGATCGATGCAGGCGTCCCTCCCGAG
GAAGCTCCTGAATATGTCTGCTTTCGCGGCACCGGTTGTGCCAAGTGCAACAACACCGGCTACAAGGGGCGCGTCGGCTT
CTATCAGGTAATGCCCATGCTGGAGGAAATCAGGGAGCTGATTCTCAACGGCGCCAATACGGCCGAAATCAAACGCGAAT
CCATGCGCCTGGGCATCAAGACCATGCGCCAATCGGGCCTGACCAAACTCAAGGAGGGGGTTACCTCCTTTGAGGAGGTG
CTACGGGTTACCGTGGCTGACGACTAA

Upstream 100 bases:

>100_bases
TGAAAGCGTGTCTCATGGCGGCTCTGATGAGCTAACTACTTCTTGACATTGGACTTTTGACGACCTACGATGGGTTCGTC
GCCAATACGCCGGAGAGTCT

Downstream 100 bases:

>100_bases
ACAAAATGCCATACGCGAGCGATCACGGCGTGGCTCTATCGCGGCTTCCTCAGCGTACTTCCCATAAGCCCCGGAGCCTC
AATCCCGCTCATCTGAGATC

Product: type IV pilus biogenesis protein PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MQASRLGELLVRNNIITKEQLAKALDEQRTSGGQQRLGSILVKNGLVTEPDLTTFLSKQYGVPSINLSEFEADMAVVKII
PADVAQKYQIVPVNRAGSTLIIAMADPSNIFAIDDIKFMTGYNVEVVVASESSIKTAIDKYYDQSASLADVMNDLEMDDL
EVIGEDEDVDVSSLERATEDAPVVKLVNLILTDAIKKKASDIHIEPYERTFRVRYRIDGVLYEVMKPPLKLKNAITSRIK
IMADLDIAERRLPQDGRIKIKMGGGQDMDYRVSVLPTLFGEKVVLRLLDKSNLQLDMTKLGYEPTALSYFKEAIHKPFGM
VLVTGPTGSGKTVSLYSALSELNKTTENISTAEDPVEFNFAGINQVQMHEDIGLTFAAALRSFLRQDPDIIMIGEIRDFE
TAEIAIKAALTGHLVLSTLHTNDAPATINRLLNMGVEPFLVASAVNLITAQRLARRVCSECKAVEEIPIQALIDAGVPPE
EAPEYVCFRGTGCAKCNNTGYKGRVGFYQVMPMLEEIRELILNGANTAEIKRESMRLGIKTMRQSGLTKLKEGVTSFEEV
LRVTVADD

Sequences:

>Translated_568_residues
MQASRLGELLVRNNIITKEQLAKALDEQRTSGGQQRLGSILVKNGLVTEPDLTTFLSKQYGVPSINLSEFEADMAVVKII
PADVAQKYQIVPVNRAGSTLIIAMADPSNIFAIDDIKFMTGYNVEVVVASESSIKTAIDKYYDQSASLADVMNDLEMDDL
EVIGEDEDVDVSSLERATEDAPVVKLVNLILTDAIKKKASDIHIEPYERTFRVRYRIDGVLYEVMKPPLKLKNAITSRIK
IMADLDIAERRLPQDGRIKIKMGGGQDMDYRVSVLPTLFGEKVVLRLLDKSNLQLDMTKLGYEPTALSYFKEAIHKPFGM
VLVTGPTGSGKTVSLYSALSELNKTTENISTAEDPVEFNFAGINQVQMHEDIGLTFAAALRSFLRQDPDIIMIGEIRDFE
TAEIAIKAALTGHLVLSTLHTNDAPATINRLLNMGVEPFLVASAVNLITAQRLARRVCSECKAVEEIPIQALIDAGVPPE
EAPEYVCFRGTGCAKCNNTGYKGRVGFYQVMPMLEEIRELILNGANTAEIKRESMRLGIKTMRQSGLTKLKEGVTSFEEV
LRVTVADD
>Mature_568_residues
MQASRLGELLVRNNIITKEQLAKALDEQRTSGGQQRLGSILVKNGLVTEPDLTTFLSKQYGVPSINLSEFEADMAVVKII
PADVAQKYQIVPVNRAGSTLIIAMADPSNIFAIDDIKFMTGYNVEVVVASESSIKTAIDKYYDQSASLADVMNDLEMDDL
EVIGEDEDVDVSSLERATEDAPVVKLVNLILTDAIKKKASDIHIEPYERTFRVRYRIDGVLYEVMKPPLKLKNAITSRIK
IMADLDIAERRLPQDGRIKIKMGGGQDMDYRVSVLPTLFGEKVVLRLLDKSNLQLDMTKLGYEPTALSYFKEAIHKPFGM
VLVTGPTGSGKTVSLYSALSELNKTTENISTAEDPVEFNFAGINQVQMHEDIGLTFAAALRSFLRQDPDIIMIGEIRDFE
TAEIAIKAALTGHLVLSTLHTNDAPATINRLLNMGVEPFLVASAVNLITAQRLARRVCSECKAVEEIPIQALIDAGVPPE
EAPEYVCFRGTGCAKCNNTGYKGRVGFYQVMPMLEEIRELILNGANTAEIKRESMRLGIKTMRQSGLTKLKEGVTSFEEV
LRVTVADD

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=419, Percent_Identity=46.0620525059666, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1786296, Length=382, Percent_Identity=46.3350785340314, Blast_Score=354, Evalue=1e-98,
Organism=Escherichia coli, GI87082188, Length=249, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62659; Mature: 62659

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQASRLGELLVRNNIITKEQLAKALDEQRTSGGQQRLGSILVKNGLVTEPDLTTFLSKQY
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHC
GVPSINLSEFEADMAVVKIIPADVAQKYQIVPVNRAGSTLIIAMADPSNIFAIDDIKFMT
CCCCCCHHHHHHCCCEEEEECHHHHCCEEEEEECCCCCEEEEEEECCCCEEEEECEEEEC
GYNVEVVVASESSIKTAIDKYYDQSASLADVMNDLEMDDLEVIGEDEDVDVSSLERATED
CCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHCCC
APVVKLVNLILTDAIKKKASDIHIEPYERTFRVRYRIDGVLYEVMKPPLKLKNAITSRIK
CHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEEEHHHHHHHHCCCHHHHHHHHHHHE
IMADLDIAERRLPQDGRIKIKMGGGQDMDYRVSVLPTLFGEKVVLRLLDKSNLQLDMTKL
EEECCCHHHHHCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEHHHC
GYEPTALSYFKEAIHKPFGMVLVTGPTGSGKTVSLYSALSELNKTTENISTAEDPVEFNF
CCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCEEEE
AGINQVQMHEDIGLTFAAALRSFLRQDPDIIMIGEIRDFETAEIAIKAALTGHLVLSTLH
CCCCEEEEHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHEEEEHHHHHHEEEEEEC
TNDAPATINRLLNMGVEPFLVASAVNLITAQRLARRVCSECKAVEEIPIQALIDAGVPPE
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCC
EAPEYVCFRGTGCAKCNNTGYKGRVGFYQVMPMLEEIRELILNGANTAEIKRESMRLGIK
CCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TMRQSGLTKLKEGVTSFEEVLRVTVADD
HHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQASRLGELLVRNNIITKEQLAKALDEQRTSGGQQRLGSILVKNGLVTEPDLTTFLSKQY
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHC
GVPSINLSEFEADMAVVKIIPADVAQKYQIVPVNRAGSTLIIAMADPSNIFAIDDIKFMT
CCCCCCHHHHHHCCCEEEEECHHHHCCEEEEEECCCCCEEEEEEECCCCEEEEECEEEEC
GYNVEVVVASESSIKTAIDKYYDQSASLADVMNDLEMDDLEVIGEDEDVDVSSLERATED
CCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHCCC
APVVKLVNLILTDAIKKKASDIHIEPYERTFRVRYRIDGVLYEVMKPPLKLKNAITSRIK
CHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEEEHHHHHHHHCCCHHHHHHHHHHHE
IMADLDIAERRLPQDGRIKIKMGGGQDMDYRVSVLPTLFGEKVVLRLLDKSNLQLDMTKL
EEECCCHHHHHCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEHHHC
GYEPTALSYFKEAIHKPFGMVLVTGPTGSGKTVSLYSALSELNKTTENISTAEDPVEFNF
CCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCEEEE
AGINQVQMHEDIGLTFAAALRSFLRQDPDIIMIGEIRDFETAEIAIKAALTGHLVLSTLH
CCCCEEEEHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHEEEEHHHHHHEEEEEEC
TNDAPATINRLLNMGVEPFLVASAVNLITAQRLARRVCSECKAVEEIPIQALIDAGVPPE
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCC
EAPEYVCFRGTGCAKCNNTGYKGRVGFYQVMPMLEEIRELILNGANTAEIKRESMRLGIK
CCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TMRQSGLTKLKEGVTSFEEVLRVTVADD
HHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]