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The map label for this gene is appA [H]

Identifier: 39996533

GI number: 39996533

Start: 1571369

End: 1572985

Strand: Direct

Name: appA [H]

Synonym: GSU1433

Alternate gene names: 39996533

Gene position: 1571369-1572985 (Clockwise)

Preceding gene: 39996532

Following gene: 39996534

Centisome position: 41.2

GC content: 62.15

Gene sequence:

>1617_bases
ATGACGTTCCGGTGGATCGGCTGCCTTCTTGCCCTGCTGTTGATCGTATCGTGCAGCGGCGAAGACCGCGCGAGAGCCCC
TGCCGTGCGCGGACCGGGTACGCCCGCTTACGGTGACGCCATCGTGGTGGGTACCATTGGCGAACCGTCGAACTTGCTTC
CTCTGCTGGCGTCCGACTCGGCATCCCACGAGGTGGCGGGCAATATCTTCAACGGCCTTGTCCGTTACGACAAGAACCTG
AACCTGGAGGGGGAACTGGCCGAGTCGTGGCAGGTTTCTCCCGACGGCCTCACCATCACTTTCCATCTCCGCAAGGGGGT
GAAATGGCACGACGGCGTGGAGTTCACCTCCCGTGACGTGCTCTACACCTACCGGGTGACGGTGGATCCGAAGACCCCCA
CTGCGTACGCCGAGGATTTCAAGCAGGTCAAGACCGCCGAGGCACCCGATCCCTACACCTTTCGCGTCACCTATGCCAAG
CCGTTCGCCCCTGCGTTGGCCTCCTGGGGGATGGGAATTCTCCCGGCTCACCTGCTGGAAGGGAAGGACATCACCAAGAG
TGAACTCTCCCGTCGTCCCGTCGGAACCGGGCCCTACCGGTTCAAGGAATGGGTCGCCGGTCAGAAGATCGTCCTCGAAT
CCAATCACGACTACTACGAGGGCCGCCCGTACATTGACCGTTACATCTACCGGATCATCCCCGACAATTCCACCATGTAC
CTGGAACTCAAGGCAGGCGGCGTGGACCTGATGGGACTCTCGCCGGTCCAGTATGCCCGGCAGACGGATACCCCCGAGTT
TCGTTCCCGCTTCGCCAAGTACCGCTATCCGGCCTCGGCCTACACCTACCTGGGGTACAATCTGAAAAATCCGCTTTTTG
CCGATCGTCGCGTGCGCCAGGCCATTGCCCATGCCCTCAGCAAGGACGAGATCATTCACGGTGTGCTCCTGGGGCTCGGC
CAGGTGGCGGTGGGCCCCTTCAAGCCGGGGACCTGGGCCTACAATCCCACGGTGCGCGACCCGGGCTATGACCCGGTGCG
GGCGAGGCAACTGCTTGCCGAGGCGGGGTGGCGCGCAACCGGGCCCGACGGCATCCTGATGAAGGACGGCAAGCCGCTGA
GCTTCACCATTCTGACGAACCAGGGCAATGACCAGCGGCTCAAGACCGCCCAGATCATCCAGCGCCGGCTGCGCAAGGTC
GGGATCGACGTGAAGATCCGGGTGCTGGAGTGGGCGTCTCTCCTGACCAATTTCATCGACAAGCGGAATTTCGATGCCCT
CATCATGGGGTGGACGATCCCCCAGGATCCCGATCTCTTCGATGTCTGGCATTCGAGCAAGACCGGTCCCAAGGAACTGA
ATTTCGTCGGCTTCAAAAACGATGAGGTGGACCGCCTGATCGAGGAGGGGCGCCGCACTTTCGACCAGGAGAAGCGCAGG
CGCTGCTACTGGCGCATCCAGGAGATTCTGGCCGAGGAGCAGCCCTACACCTTTTTGTACGTCCCCGATGCCCTGCCGGC
GGTGAGCGCCCGGTTCAGGGGAATCGAGCCCGCGCCGGCCGGCATCATGTACAACTTCATCCGTTGGTACGTGCCGAAAG
AAGAGCAGGTGCACTAA

Upstream 100 bases:

>100_bases
GCAAGGCCCTTGAACTCTACCGGAAGATCCTGGAAACGGAGCCCGGCCGCAAGGATATCATCGAGAAGAAGCGCAAGGTC
AGGGGGGAGAGCCAGGAAAA

Downstream 100 bases:

>100_bases
CATGGCCGCCTACATCTTCAAGCGACTCCTGGCAATGATCCCGTTGCTGTTCGGGATCACCCTCATTACCTTCGTGGTGA
TCCGGCTCGCCCCCGGGGAG

Product: peptide ABC transporter periplasmic peptide-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MTFRWIGCLLALLLIVSCSGEDRARAPAVRGPGTPAYGDAIVVGTIGEPSNLLPLLASDSASHEVAGNIFNGLVRYDKNL
NLEGELAESWQVSPDGLTITFHLRKGVKWHDGVEFTSRDVLYTYRVTVDPKTPTAYAEDFKQVKTAEAPDPYTFRVTYAK
PFAPALASWGMGILPAHLLEGKDITKSELSRRPVGTGPYRFKEWVAGQKIVLESNHDYYEGRPYIDRYIYRIIPDNSTMY
LELKAGGVDLMGLSPVQYARQTDTPEFRSRFAKYRYPASAYTYLGYNLKNPLFADRRVRQAIAHALSKDEIIHGVLLGLG
QVAVGPFKPGTWAYNPTVRDPGYDPVRARQLLAEAGWRATGPDGILMKDGKPLSFTILTNQGNDQRLKTAQIIQRRLRKV
GIDVKIRVLEWASLLTNFIDKRNFDALIMGWTIPQDPDLFDVWHSSKTGPKELNFVGFKNDEVDRLIEEGRRTFDQEKRR
RCYWRIQEILAEEQPYTFLYVPDALPAVSARFRGIEPAPAGIMYNFIRWYVPKEEQVH

Sequences:

>Translated_538_residues
MTFRWIGCLLALLLIVSCSGEDRARAPAVRGPGTPAYGDAIVVGTIGEPSNLLPLLASDSASHEVAGNIFNGLVRYDKNL
NLEGELAESWQVSPDGLTITFHLRKGVKWHDGVEFTSRDVLYTYRVTVDPKTPTAYAEDFKQVKTAEAPDPYTFRVTYAK
PFAPALASWGMGILPAHLLEGKDITKSELSRRPVGTGPYRFKEWVAGQKIVLESNHDYYEGRPYIDRYIYRIIPDNSTMY
LELKAGGVDLMGLSPVQYARQTDTPEFRSRFAKYRYPASAYTYLGYNLKNPLFADRRVRQAIAHALSKDEIIHGVLLGLG
QVAVGPFKPGTWAYNPTVRDPGYDPVRARQLLAEAGWRATGPDGILMKDGKPLSFTILTNQGNDQRLKTAQIIQRRLRKV
GIDVKIRVLEWASLLTNFIDKRNFDALIMGWTIPQDPDLFDVWHSSKTGPKELNFVGFKNDEVDRLIEEGRRTFDQEKRR
RCYWRIQEILAEEQPYTFLYVPDALPAVSARFRGIEPAPAGIMYNFIRWYVPKEEQVH
>Mature_537_residues
TFRWIGCLLALLLIVSCSGEDRARAPAVRGPGTPAYGDAIVVGTIGEPSNLLPLLASDSASHEVAGNIFNGLVRYDKNLN
LEGELAESWQVSPDGLTITFHLRKGVKWHDGVEFTSRDVLYTYRVTVDPKTPTAYAEDFKQVKTAEAPDPYTFRVTYAKP
FAPALASWGMGILPAHLLEGKDITKSELSRRPVGTGPYRFKEWVAGQKIVLESNHDYYEGRPYIDRYIYRIIPDNSTMYL
ELKAGGVDLMGLSPVQYARQTDTPEFRSRFAKYRYPASAYTYLGYNLKNPLFADRRVRQAIAHALSKDEIIHGVLLGLGQ
VAVGPFKPGTWAYNPTVRDPGYDPVRARQLLAEAGWRATGPDGILMKDGKPLSFTILTNQGNDQRLKTAQIIQRRLRKVG
IDVKIRVLEWASLLTNFIDKRNFDALIMGWTIPQDPDLFDVWHSSKTGPKELNFVGFKNDEVDRLIEEGRRTFDQEKRRR
CYWRIQEILAEEQPYTFLYVPDALPAVSARFRGIEPAPAGIMYNFIRWYVPKEEQVH

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787762, Length=489, Percent_Identity=28.4253578732106, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1789966, Length=510, Percent_Identity=27.843137254902, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1787052, Length=488, Percent_Identity=27.0491803278689, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1789887, Length=443, Percent_Identity=27.9909706546275, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1787551, Length=564, Percent_Identity=24.822695035461, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789397, Length=522, Percent_Identity=25.6704980842912, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI87081878, Length=539, Percent_Identity=25.0463821892393, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1787495, Length=528, Percent_Identity=24.0530303030303, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87082063, Length=433, Percent_Identity=24.9422632794457, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1786256, Length=268, Percent_Identity=26.4925373134328, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60957; Mature: 60826

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFRWIGCLLALLLIVSCSGEDRARAPAVRGPGTPAYGDAIVVGTIGEPSNLLPLLASDS
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCC
ASHEVAGNIFNGLVRYDKNLNLEGELAESWQVSPDGLTITFHLRKGVKWHDGVEFTSRDV
CCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCE
LYTYRVTVDPKTPTAYAEDFKQVKTAEAPDPYTFRVTYAKPFAPALASWGMGILPAHLLE
EEEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHCCCCHHHHHHHC
GKDITKSELSRRPVGTGPYRFKEWVAGQKIVLESNHDYYEGRPYIDRYIYRIIPDNSTMY
CCCCCHHHHHCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCHHHEEEEEEECCCCEEE
LELKAGGVDLMGLSPVQYARQTDTPEFRSRFAKYRYPASAYTYLGYNLKNPLFADRRVRQ
EEEECCCEEEECCCHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHH
AIAHALSKDEIIHGVLLGLGQVAVGPFKPGTWAYNPTVRDPGYDPVRARQLLAEAGWRAT
HHHHHHCHHHHHHHHHHHHHHHEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCC
GPDGILMKDGKPLSFTILTNQGNDQRLKTAQIIQRRLRKVGIDVKIRVLEWASLLTNFID
CCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHH
KRNFDALIMGWTIPQDPDLFDVWHSSKTGPKELNFVGFKNDEVDRLIEEGRRTFDQEKRR
CCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
RCYWRIQEILAEEQPYTFLYVPDALPAVSARFRGIEPAPAGIMYNFIRWYVPKEEQVH
HHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TFRWIGCLLALLLIVSCSGEDRARAPAVRGPGTPAYGDAIVVGTIGEPSNLLPLLASDS
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCC
ASHEVAGNIFNGLVRYDKNLNLEGELAESWQVSPDGLTITFHLRKGVKWHDGVEFTSRDV
CCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCE
LYTYRVTVDPKTPTAYAEDFKQVKTAEAPDPYTFRVTYAKPFAPALASWGMGILPAHLLE
EEEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHCCCCHHHHHHHC
GKDITKSELSRRPVGTGPYRFKEWVAGQKIVLESNHDYYEGRPYIDRYIYRIIPDNSTMY
CCCCCHHHHHCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCHHHEEEEEEECCCCEEE
LELKAGGVDLMGLSPVQYARQTDTPEFRSRFAKYRYPASAYTYLGYNLKNPLFADRRVRQ
EEEECCCEEEECCCHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHH
AIAHALSKDEIIHGVLLGLGQVAVGPFKPGTWAYNPTVRDPGYDPVRARQLLAEAGWRAT
HHHHHHCHHHHHHHHHHHHHHHEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCC
GPDGILMKDGKPLSFTILTNQGNDQRLKTAQIIQRRLRKVGIDVKIRVLEWASLLTNFID
CCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHH
KRNFDALIMGWTIPQDPDLFDVWHSSKTGPKELNFVGFKNDEVDRLIEEGRRTFDQEKRR
CCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
RCYWRIQEILAEEQPYTFLYVPDALPAVSARFRGIEPAPAGIMYNFIRWYVPKEEQVH
HHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]