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The map label for this gene is appB [H]

Identifier: 39996534

GI number: 39996534

Start: 1572987

End: 1573967

Strand: Direct

Name: appB [H]

Synonym: GSU1434

Alternate gene names: 39996534

Gene position: 1572987-1573967 (Clockwise)

Preceding gene: 39996533

Following gene: 39996535

Centisome position: 41.24

GC content: 61.06

Gene sequence:

>981_bases
ATGGCCGCCTACATCTTCAAGCGACTCCTGGCAATGATCCCGTTGCTGTTCGGGATCACCCTCATTACCTTCGTGGTGAT
CCGGCTCGCCCCCGGGGAGCCGGTGGAGGCGATGACCGCCATGAGCCCCAAGATCACCGCCGAAACCCGCGAGCGGCTCC
GGGAGTTCTACGGACTCGACAAGCCGCTCCATGAGCAGTACGTTTCCTGGGTCGGCCGCATCGTTAAGCTCGACTTCGGC
CGCTCCTTCGCCCCGGACAACCGGCCGGTGACCGACAAGATCGCCGAGAGAATACCGGTGACCCTCTCCCTGAACATCAT
TGCGCTGATCCTTGAATTCGGTCTCGCCATCCCCATCGGTATCTTTGCCGCCACACACCGGGACACGATCTATGACAAGG
GAATAACCCTCTTCGTCTTTCTCGGGTTCGCCGTGCCGACCTTCTGGCTGGCGCTGCTGCTGATGTATTTTTTCGGGGTG
AAACTGGGGTGGCTTCCCATTTCGGGGCTCCACTCCCTGGGGAGCGAGAACCTTTCACCCCTGGCATGGTTCGCGGATAT
GGTAAAGCACTTGGTGCTGCCCGTGTTCGTGGCCACTTTCGGCAGTCTGGCGGGGCTGTCGCGCTATATGCGCTCGAACA
TGCTGGAGGTGATCCGCCAGGACTATATCACCACGGCCCGGGCCAAGGGGTTGTCCGAGCGTGTGGTCATTTACCGCCAC
GCCCTGCGCAACGCACTGCTGCCCGTCATCACGCTTCTGGGTTTTTCCCTGCCGGGGCTCATCGGAGGGAGTGTCATCTT
CGAGACGATTTTCGCCATCCCGGGCATGGGACAGCTCTTCTACCAAGGGGTCATGGCCCGCGACTATCCGCTTGTCATGG
GGATTCTCGTCATCGGCGCGTTCCTTACGCTCATCGGTAACCTGCTGGCCGATGTCTGCTATGCCATGGCCGATCCGCGC
ATCCGGCACGGAAGGGGGTAG

Upstream 100 bases:

>100_bases
GCGGTGAGCGCCCGGTTCAGGGGAATCGAGCCCGCGCCGGCCGGCATCATGTACAACTTCATCCGTTGGTACGTGCCGAA
AGAAGAGCAGGTGCACTAAC

Downstream 100 bases:

>100_bases
GGGATGGCCTACCGCGACAGCTGGTTCCATACGGTCTTCTGGAAACGTTTCCGGCGCAACCGTTTCGCCCTGGCCGGCGG
CGTGGTTGTGGTGGTGCTCT

Product: peptide ABC transporter permease

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MAAYIFKRLLAMIPLLFGITLITFVVIRLAPGEPVEAMTAMSPKITAETRERLREFYGLDKPLHEQYVSWVGRIVKLDFG
RSFAPDNRPVTDKIAERIPVTLSLNIIALILEFGLAIPIGIFAATHRDTIYDKGITLFVFLGFAVPTFWLALLLMYFFGV
KLGWLPISGLHSLGSENLSPLAWFADMVKHLVLPVFVATFGSLAGLSRYMRSNMLEVIRQDYITTARAKGLSERVVIYRH
ALRNALLPVITLLGFSLPGLIGGSVIFETIFAIPGMGQLFYQGVMARDYPLVMGILVIGAFLTLIGNLLADVCYAMADPR
IRHGRG

Sequences:

>Translated_326_residues
MAAYIFKRLLAMIPLLFGITLITFVVIRLAPGEPVEAMTAMSPKITAETRERLREFYGLDKPLHEQYVSWVGRIVKLDFG
RSFAPDNRPVTDKIAERIPVTLSLNIIALILEFGLAIPIGIFAATHRDTIYDKGITLFVFLGFAVPTFWLALLLMYFFGV
KLGWLPISGLHSLGSENLSPLAWFADMVKHLVLPVFVATFGSLAGLSRYMRSNMLEVIRQDYITTARAKGLSERVVIYRH
ALRNALLPVITLLGFSLPGLIGGSVIFETIFAIPGMGQLFYQGVMARDYPLVMGILVIGAFLTLIGNLLADVCYAMADPR
IRHGRG
>Mature_325_residues
AAYIFKRLLAMIPLLFGITLITFVVIRLAPGEPVEAMTAMSPKITAETRERLREFYGLDKPLHEQYVSWVGRIVKLDFGR
SFAPDNRPVTDKIAERIPVTLSLNIIALILEFGLAIPIGIFAATHRDTIYDKGITLFVFLGFAVPTFWLALLLMYFFGVK
LGWLPISGLHSLGSENLSPLAWFADMVKHLVLPVFVATFGSLAGLSRYMRSNMLEVIRQDYITTARAKGLSERVVIYRHA
LRNALLPVITLLGFSLPGLIGGSVIFETIFAIPGMGQLFYQGVMARDYPLVMGILVIGAFLTLIGNLLADVCYAMADPRI
RHGRG

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=331, Percent_Identity=38.0664652567976, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1789965, Length=341, Percent_Identity=36.6568914956012, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1787497, Length=323, Percent_Identity=35.6037151702786, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1787053, Length=323, Percent_Identity=34.984520123839, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI1788504, Length=369, Percent_Identity=30.8943089430894, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1787761, Length=334, Percent_Identity=34.4311377245509, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787550, Length=279, Percent_Identity=32.9749103942652, Blast_Score=153, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36180; Mature: 36049

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAYIFKRLLAMIPLLFGITLITFVVIRLAPGEPVEAMTAMSPKITAETRERLREFYGLD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCC
KPLHEQYVSWVGRIVKLDFGRSFAPDNRPVTDKIAERIPVTLSLNIIALILEFGLAIPIG
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IFAATHRDTIYDKGITLFVFLGFAVPTFWLALLLMYFFGVKLGWLPISGLHSLGSENLSP
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCH
LAWFADMVKHLVLPVFVATFGSLAGLSRYMRSNMLEVIRQDYITTARAKGLSERVVIYRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ALRNALLPVITLLGFSLPGLIGGSVIFETIFAIPGMGQLFYQGVMARDYPLVMGILVIGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHH
FLTLIGNLLADVCYAMADPRIRHGRG
HHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AAYIFKRLLAMIPLLFGITLITFVVIRLAPGEPVEAMTAMSPKITAETRERLREFYGLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCC
KPLHEQYVSWVGRIVKLDFGRSFAPDNRPVTDKIAERIPVTLSLNIIALILEFGLAIPIG
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IFAATHRDTIYDKGITLFVFLGFAVPTFWLALLLMYFFGVKLGWLPISGLHSLGSENLSP
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCH
LAWFADMVKHLVLPVFVATFGSLAGLSRYMRSNMLEVIRQDYITTARAKGLSERVVIYRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ALRNALLPVITLLGFSLPGLIGGSVIFETIFAIPGMGQLFYQGVMARDYPLVMGILVIGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHH
FLTLIGNLLADVCYAMADPRIRHGRG
HHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]