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The map label for this gene is rlmN

Identifier: 39996213

GI number: 39996213

Start: 1196753

End: 1197835

Strand: Direct

Name: rlmN

Synonym: GSU1111

Alternate gene names: 39996213

Gene position: 1196753-1197835 (Clockwise)

Preceding gene: 39996212

Following gene: 39996214

Centisome position: 31.38

GC content: 56.42

Gene sequence:

>1083_bases
ATGGATACAATGATTGACATTAAGGGGCTGAGCATTGACGAGCTGGAACGTTTCCTCCTGGGAAAAGGGAAGGAGCGTTA
CCGGGCACGCCAGATATTCAAGTGGCTGTATCAACGGGGTGCCACCTCCTTTGCCGAGATGACTGATCTGGCGAAGGAGT
TGCGTCGGGACCTTGAGGAGACGGCACGCATCAGCACGCTTTCCCCCGAGGCCTTGGAGATTTCCCGGGATGGAACGCGA
AAATACCTCTTCCGTCTCGATGATGGATGTTCAGTGGAGTCGGTGCTCATCCCGGAAGAAGACAGAAACACCCTTTGTAT
TTCGAGCCAAGTAGGGTGTGCCATGGCCTGTGAGTTCTGTCTCACGGGAACGTTCCGGCTGACGCGCAACCTGACCACCG
CCGAGATCGTAAATCAGGTCTGTGCGGTTCAGAGGGATGTCCCCGTGCGCAACATCGTCTTCATGGGCATGGGAGAACCC
CTCGCCAATCTCGACAATGTGATCAGGGCGCTGCAGATCATGCTGCACGATGACGGACTTCAGTTCTCGACGCGTCGCAT
AACCGTTTCGACCGCCGGCTTGGTACCGGAAATGGAGCGGCTCGGCCGATCGGTAACGGTGAATCTTGCGGTTTCGCTCA
ATGCCACTACGGACGAGCTGCGCGACCGGATCATGCCGATCAACCGGAAATATCCCCTTGCCGTGCTTCTTGATGCCTGT
CGCCGGTTCCCGCTGCCGGGCCGTCGGAAGATAACTATTGAATACGTGCTGCTCGGCGGGGTGAACGATACCCTCGATGA
TGCCAAACGGCTGGTCAGGCTGTTGAGCGACATTCCCTCTAAAATCAATCTGATCCCCTTTAATGAACATGAGGGATGTT
CCTTCCGGAGCCCCAGCCAGGATGCGATCGACCGTTTTCACAGATATCTGCTGGACAAGCATTTCACGGTCATAACGCGC
TCCAGCCGTGGTGCCGATATTTCGGCGGCCTGCGGTCAGCTCAAGGGCAAGCTGGACGCATTGAAGCCGTCCGGCGCGGG
AAAGCAGATAGAGGTTTCAGACTCCACTAACGAGGTGACATGA

Upstream 100 bases:

>100_bases
TCCGTATTTCTTCAGCCAGCTCGAGCTGCTCTGAAGCTGAATGCGTGTGTGTCAGCAAAGCCCTTCTCTCGCGAAGGGCT
TTTTTTTATAGTACGCTACC

Downstream 100 bases:

>100_bases
CATGGAACAGGTAATCGGCGTTATCGGCGGGAGCGGGCTCTACGAGATGGAAGGGCTTCAGGACGTTCGGAGCATTGTTG
TTGAAACCCCCTTCGGCGCA

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MDTMIDIKGLSIDELERFLLGKGKERYRARQIFKWLYQRGATSFAEMTDLAKELRRDLEETARISTLSPEALEISRDGTR
KYLFRLDDGCSVESVLIPEEDRNTLCISSQVGCAMACEFCLTGTFRLTRNLTTAEIVNQVCAVQRDVPVRNIVFMGMGEP
LANLDNVIRALQIMLHDDGLQFSTRRITVSTAGLVPEMERLGRSVTVNLAVSLNATTDELRDRIMPINRKYPLAVLLDAC
RRFPLPGRRKITIEYVLLGGVNDTLDDAKRLVRLLSDIPSKINLIPFNEHEGCSFRSPSQDAIDRFHRYLLDKHFTVITR
SSRGADISAACGQLKGKLDALKPSGAGKQIEVSDSTNEVT

Sequences:

>Translated_360_residues
MDTMIDIKGLSIDELERFLLGKGKERYRARQIFKWLYQRGATSFAEMTDLAKELRRDLEETARISTLSPEALEISRDGTR
KYLFRLDDGCSVESVLIPEEDRNTLCISSQVGCAMACEFCLTGTFRLTRNLTTAEIVNQVCAVQRDVPVRNIVFMGMGEP
LANLDNVIRALQIMLHDDGLQFSTRRITVSTAGLVPEMERLGRSVTVNLAVSLNATTDELRDRIMPINRKYPLAVLLDAC
RRFPLPGRRKITIEYVLLGGVNDTLDDAKRLVRLLSDIPSKINLIPFNEHEGCSFRSPSQDAIDRFHRYLLDKHFTVITR
SSRGADISAACGQLKGKLDALKPSGAGKQIEVSDSTNEVT
>Mature_360_residues
MDTMIDIKGLSIDELERFLLGKGKERYRARQIFKWLYQRGATSFAEMTDLAKELRRDLEETARISTLSPEALEISRDGTR
KYLFRLDDGCSVESVLIPEEDRNTLCISSQVGCAMACEFCLTGTFRLTRNLTTAEIVNQVCAVQRDVPVRNIVFMGMGEP
LANLDNVIRALQIMLHDDGLQFSTRRITVSTAGLVPEMERLGRSVTVNLAVSLNATTDELRDRIMPINRKYPLAVLLDAC
RRFPLPGRRKITIEYVLLGGVNDTLDDAKRLVRLLSDIPSKINLIPFNEHEGCSFRSPSQDAIDRFHRYLLDKHFTVITR
SSRGADISAACGQLKGKLDALKPSGAGKQIEVSDSTNEVT

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=351, Percent_Identity=44.7293447293447, Blast_Score=293, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_GEOSL (Q74E53)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952164.1
- ProteinModelPortal:   Q74E53
- GeneID:   2688552
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1111
- NMPDR:   fig|243231.1.peg.1102
- TIGR:   GSU1111
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK14463
- BioCyc:   GSUL243231:GSU_1111-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 40373; Mature: 40373

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTMIDIKGLSIDELERFLLGKGKERYRARQIFKWLYQRGATSFAEMTDLAKELRRDLEE
CCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
TARISTLSPEALEISRDGTRKYLFRLDDGCSVESVLIPEEDRNTLCISSQVGCAMACEFC
HHHHCCCCCCCEEECCCCCEEEEEEECCCCCCCEEECCCCCCCEEEEECCCCHHHHHHHH
LTGTFRLTRNLTTAEIVNQVCAVQRDVPVRNIVFMGMGEPLANLDNVIRALQIMLHDDGL
HCCHHHHHCCCHHHHHHHHHHHHHCCCCHHEEEEEECCCCHHHHHHHHHHHHHHHCCCCC
QFSTRRITVSTAGLVPEMERLGRSVTVNLAVSLNATTDELRDRIMPINRKYPLAVLLDAC
EEEEEEEEEECCCCCHHHHHCCCEEEEEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHH
RRFPLPGRRKITIEYVLLGGVNDTLDDAKRLVRLLSDIPSKINLIPFNEHEGCSFRSPSQ
HCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCH
DAIDRFHRYLLDKHFTVITRSSRGADISAACGQLKGKLDALKPSGAGKQIEVSDSTNEVT
HHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCHHCCCCCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MDTMIDIKGLSIDELERFLLGKGKERYRARQIFKWLYQRGATSFAEMTDLAKELRRDLEE
CCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
TARISTLSPEALEISRDGTRKYLFRLDDGCSVESVLIPEEDRNTLCISSQVGCAMACEFC
HHHHCCCCCCCEEECCCCCEEEEEEECCCCCCCEEECCCCCCCEEEEECCCCHHHHHHHH
LTGTFRLTRNLTTAEIVNQVCAVQRDVPVRNIVFMGMGEPLANLDNVIRALQIMLHDDGL
HCCHHHHHCCCHHHHHHHHHHHHHCCCCHHEEEEEECCCCHHHHHHHHHHHHHHHCCCCC
QFSTRRITVSTAGLVPEMERLGRSVTVNLAVSLNATTDELRDRIMPINRKYPLAVLLDAC
EEEEEEEEEECCCCCHHHHHCCCEEEEEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHH
RRFPLPGRRKITIEYVLLGGVNDTLDDAKRLVRLLSDIPSKINLIPFNEHEGCSFRSPSQ
HCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCH
DAIDRFHRYLLDKHFTVITRSSRGADISAACGQLKGKLDALKPSGAGKQIEVSDSTNEVT
HHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCHHCCCCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA