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The map label for this gene is trmD

Identifier: 39995752

GI number: 39995752

Start: 678959

End: 679696

Strand: Direct

Name: trmD

Synonym: GSU0646

Alternate gene names: 39995752

Gene position: 678959-679696 (Clockwise)

Preceding gene: 39995751

Following gene: 39995753

Centisome position: 17.8

GC content: 60.3

Gene sequence:

>738_bases
ATGAAATTTGACATATTGACGCTGTTTCCAGCCATGTTCGACGGGCCTCTTACCGAAAGCATCATCCGGCGGGCCGTGGA
ACGGGGGTTCGTGGATATCAGTCTGCACAATATTCGCGACTACGCAACCGATCGGCACCGGGTCGTTGATGATGCCCCGT
ACGGCGGCGGTGATGGCATGGTCATGAAAGTCGAGCCTCTTGCCGCCTGCATTGAGGCGGTGAAGGCAGAGCGGCCGGCA
GCGCGGGTCATCCTGACCACCCCCCGGGGGCGGCGATTCGACCATGACGCCGCCCGCGAGCTGGCCGGCTACGAAGAGCT
CATCATCGTTTGCGGCCGTTATGAAGGAATAGACGAACGGGTGCGCGATCTCTTTGTGGATGACGAATTTTCCATCGGCG
ATTTCGTCCTCACGGGAGGCGAACTTGCCGCAATGGTCATGATCGACGCGGTGATCCGTTTTATCCCCGGCGTCCTCGGT
TCTCCCGGCTCGGCGGAATATGATTCATTCGCTGACGGCCTGCTCGAATATCCTCAGTACACCCGTCCGGTGGAGTTCCG
GGGGGTTCAAGTGCCAGGGGTCCTCCTGTCGGGAAACCACGCCGAGATCAGCCGATGGCGGCGCCAGAAAGCTCTTGAGT
TGACGAGGCGGGTGCGTCCCGATCTGCTCCACAAAGCGGAACTAAGCAGCAGTGACCGGGACTGTCTGGCGATGCTCGAA
AGGGAAGGCGAGCCATGA

Upstream 100 bases:

>100_bases
ACAGGGAATATCTCATCCCGGCCGTAGAGGATGTGGTGGTGCAGGTCGACCTTGAGGCAGGCACCATGACGGTCAGTCCC
CCCGAGGGGCTGCTCGATCT

Downstream 100 bases:

>100_bases
CCTGCGGTGAGTGCAGCAGGCCTGCGGTGAGTGTGGCTCTTGTCCACTATCCGGTGTATGACAAGAATCGGCAGGTAGTC
GCAACGGCCGTCACCAATCT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MKFDILTLFPAMFDGPLTESIIRRAVERGFVDISLHNIRDYATDRHRVVDDAPYGGGDGMVMKVEPLAACIEAVKAERPA
ARVILTTPRGRRFDHDAARELAGYEELIIVCGRYEGIDERVRDLFVDDEFSIGDFVLTGGELAAMVMIDAVIRFIPGVLG
SPGSAEYDSFADGLLEYPQYTRPVEFRGVQVPGVLLSGNHAEISRWRRQKALELTRRVRPDLLHKAELSSSDRDCLAMLE
REGEP

Sequences:

>Translated_245_residues
MKFDILTLFPAMFDGPLTESIIRRAVERGFVDISLHNIRDYATDRHRVVDDAPYGGGDGMVMKVEPLAACIEAVKAERPA
ARVILTTPRGRRFDHDAARELAGYEELIIVCGRYEGIDERVRDLFVDDEFSIGDFVLTGGELAAMVMIDAVIRFIPGVLG
SPGSAEYDSFADGLLEYPQYTRPVEFRGVQVPGVLLSGNHAEISRWRRQKALELTRRVRPDLLHKAELSSSDRDCLAMLE
REGEP
>Mature_245_residues
MKFDILTLFPAMFDGPLTESIIRRAVERGFVDISLHNIRDYATDRHRVVDDAPYGGGDGMVMKVEPLAACIEAVKAERPA
ARVILTTPRGRRFDHDAARELAGYEELIIVCGRYEGIDERVRDLFVDDEFSIGDFVLTGGELAAMVMIDAVIRFIPGVLG
SPGSAEYDSFADGLLEYPQYTRPVEFRGVQVPGVLLSGNHAEISRWRRQKALELTRRVRPDLLHKAELSSSDRDCLAMLE
REGEP

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=243, Percent_Identity=51.0288065843621, Blast_Score=227, Evalue=5e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_GEOSL (Q74FG3)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951703.1
- ProteinModelPortal:   Q74FG3
- SMR:   Q74FG3
- GeneID:   2687328
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0646
- NMPDR:   fig|243231.1.peg.641
- TIGR:   GSU0646
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   GSUL243231:GSU_0646-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27396; Mature: 27396

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFDILTLFPAMFDGPLTESIIRRAVERGFVDISLHNIRDYATDRHRVVDDAPYGGGDGM
CCCCHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCHHCCCCCCCCCCCCE
VMKVEPLAACIEAVKAERPAARVILTTPRGRRFDHDAARELAGYEELIIVCGRYEGIDER
EEEEHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHCCHHHEEEEECCCCCHHHH
VRDLFVDDEFSIGDFVLTGGELAAMVMIDAVIRFIPGVLGSPGSAEYDSFADGLLEYPQY
HHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
TRPVEFRGVQVPGVLLSGNHAEISRWRRQKALELTRRVRPDLLHKAELSSSDRDCLAMLE
CCCCCCCCEECCCEEEECCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHH
REGEP
HCCCC
>Mature Secondary Structure
MKFDILTLFPAMFDGPLTESIIRRAVERGFVDISLHNIRDYATDRHRVVDDAPYGGGDGM
CCCCHHHHHHHHHCCHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCHHCCCCCCCCCCCCE
VMKVEPLAACIEAVKAERPAARVILTTPRGRRFDHDAARELAGYEELIIVCGRYEGIDER
EEEEHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHCCHHHEEEEECCCCCHHHH
VRDLFVDDEFSIGDFVLTGGELAAMVMIDAVIRFIPGVLGSPGSAEYDSFADGLLEYPQY
HHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
TRPVEFRGVQVPGVLLSGNHAEISRWRRQKALELTRRVRPDLLHKAELSSSDRDCLAMLE
CCCCCCCCEECCCEEEECCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHH
REGEP
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA