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The map label for this gene is nfsA [C]

Identifier: 39995327

GI number: 39995327

Start: 226424

End: 226990

Strand: Direct

Name: nfsA [C]

Synonym: GSU0217

Alternate gene names: 39995327

Gene position: 226424-226990 (Clockwise)

Preceding gene: 39995325

Following gene: 39995328

Centisome position: 5.94

GC content: 64.9

Gene sequence:

>567_bases
ATGGATACTCTTGAGGCAATCAGGACGCGACGGAGCGTGCGGGCATTTTCAGACCGGCCGGTGGAGCCGGAAAAGCTTCA
GATGGTGCTGGAGGCGGCACGTCAGGCGCCCTCCTGGGCAAACATGCAGTGCAGCCGCTTCGTGGTGGTGCAGGATGCCG
AAGTCAAGGCAAAGATCAGCGAGCTGTCGTTCGTGGAGGCATTTTTCGCGCCGCTCGGCTACAGGACCAACCCGGCCCAG
AAGGCGCTCGCCGAAGCGCCGGTGGTGATCGTTGCCTGCGGCGTGCCCGGCGAGTCGGGCGATCTGCGCGGGCAGCAGTA
CTACATGACGGACGTGGGGATCGCCACGGAAAACCTCATGCTCGCCGCCCATGCCGTGGGACTCGGCTCGGTGTTTGTCG
GCGTCTTCGGCGAGGAACAGCTCGGCGACCTCCTGGACATCCCGCCGGAGATCAGGATCGTTGGGCTTTTCCCCCTGGGC
TATCCCCGGGAGGAGACGCAGGCGAAGTCGGGCCCGGCGCGCAAGCCCCTGGATGAGATCGTGTTCCAGGGCAAGTGGAA
GTCCTAG

Upstream 100 bases:

>100_bases
GTTCTTTGTTTTGAGCGGGCGTTTTCTCTCGGGGAGAGCCCGGTCCGTCGATCCCCTGGGGCGAGCAAAAACCGGTATGA
ACCAACAAGGAGGCATGCAA

Downstream 100 bases:

>100_bases
CCGGCCGTTGGAAAGAACGCCGGTGCGGGACCGGCAGAACAGACCGCCCCCGCACCGGCCCTCGACAGGTTCCGGCGTCC
ATGACGGAGGGAAGGGTGCT

Product: nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MDTLEAIRTRRSVRAFSDRPVEPEKLQMVLEAARQAPSWANMQCSRFVVVQDAEVKAKISELSFVEAFFAPLGYRTNPAQ
KALAEAPVVIVACGVPGESGDLRGQQYYMTDVGIATENLMLAAHAVGLGSVFVGVFGEEQLGDLLDIPPEIRIVGLFPLG
YPREETQAKSGPARKPLDEIVFQGKWKS

Sequences:

>Translated_188_residues
MDTLEAIRTRRSVRAFSDRPVEPEKLQMVLEAARQAPSWANMQCSRFVVVQDAEVKAKISELSFVEAFFAPLGYRTNPAQ
KALAEAPVVIVACGVPGESGDLRGQQYYMTDVGIATENLMLAAHAVGLGSVFVGVFGEEQLGDLLDIPPEIRIVGLFPLG
YPREETQAKSGPARKPLDEIVFQGKWKS
>Mature_188_residues
MDTLEAIRTRRSVRAFSDRPVEPEKLQMVLEAARQAPSWANMQCSRFVVVQDAEVKAKISELSFVEAFFAPLGYRTNPAQ
KALAEAPVVIVACGVPGESGDLRGQQYYMTDVGIATENLMLAAHAVGLGSVFVGVFGEEQLGDLLDIPPEIRIVGLFPLG
YPREETQAKSGPARKPLDEIVFQGKWKS

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 20565; Mature: 20565

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLEAIRTRRSVRAFSDRPVEPEKLQMVLEAARQAPSWANMQCSRFVVVQDAEVKAKIS
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHH
ELSFVEAFFAPLGYRTNPAQKALAEAPVVIVACGVPGESGDLRGQQYYMTDVGIATENLM
HHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCCCHHHHH
LAAHAVGLGSVFVGVFGEEQLGDLLDIPPEIRIVGLFPLGYPREETQAKSGPARKPLDEI
HHHHHHHHHHHHHEECCCHHHHHHHCCCCCEEEEEEECCCCCCHHHHCCCCCCCCCHHHH
VFQGKWKS
HCCCCCCC
>Mature Secondary Structure
MDTLEAIRTRRSVRAFSDRPVEPEKLQMVLEAARQAPSWANMQCSRFVVVQDAEVKAKIS
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCHHHHHHH
ELSFVEAFFAPLGYRTNPAQKALAEAPVVIVACGVPGESGDLRGQQYYMTDVGIATENLM
HHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCCCHHHHH
LAAHAVGLGSVFVGVFGEEQLGDLLDIPPEIRIVGLFPLGYPREETQAKSGPARKPLDEI
HHHHHHHHHHHHHEECCCHHHHHHHCCCCCEEEEEEECCCCCCHHHHCCCCCCCCCHHHH
VFQGKWKS
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]