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The map label for this gene is folD-1

Identifier: 39995325

GI number: 39995325

Start: 224725

End: 225597

Strand: Direct

Name: folD-1

Synonym: GSU0215

Alternate gene names: 39995325

Gene position: 224725-225597 (Clockwise)

Preceding gene: 39995324

Following gene: 39995327

Centisome position: 5.89

GC content: 58.65

Gene sequence:

>873_bases
ATGAATCTGCTGGACGGAAAGAAGTGTGCCGAGAGCCTGGTGGCCGAGATTGCCAGGAAAGTGGCGGGTTACACGGAGTC
GGGGCTGCGCACGCCCCACATGACCATCATTCTGGTAGGCGAGCACGCGCCGAGTGAATCGTATGTGAAATCCAAGATCG
CCACCAGCGGCCTCGCCGGTTTCGAGAGCACATTGCTGAGGTTCCCCGAGACAATCAGGGAGTCGGAGTTGCTGGCAAAG
ATCAAGGAGGTTAACGAAGACCCCACCACCGACGGGCTCATCGTCCAGCTGCCCCTGCCGAAACACATCAACCAGCAGCA
TGTCATCAACGCCATTGCGCCGGAAAAGGACATCGACGGATTCCATCCGGCAAACTTCGGCCGCATGACCCTGGGGCAGA
AGGCGTTCCGTCCGGCCACCGCCTACGGCATCTGCAAGCTGCTGCAGTTCTACGAGGTGCCGGTAAAGGCAAAGCACTGC
GTCGTGATCGGCCGGTCCAACATCGTGGGCAAGCCGATCTCCATCATGCTCTCCAACGACTTCGATATCGGCAACGCCAC
GGTGACGCTCACCCATATCGAAACTCCGCGCGAGCTCCTGCTGGATGAAACACGCCGGGCGGACATCGTGATCGTTGCCG
TGGGCATCCCGGGGTTTGTTACGGCCGATATGGTCAAGGATGGGGTCGTGCTGATAGACGTGGGGATCAATCGTCTGGAG
AGCGGCAAGATTGTGGGGGATGTGGATTTCGAAAATGTGGCGCAGAAATGTTCCTGGATCACGCCGGTCCCCGGTGGTGT
GGGGAGGATGACCGTTGCCGCCCTGATGATCAATACGCTCATGGCCTACCAGAATAATTTCAATCTGGCCTGA

Upstream 100 bases:

>100_bases
GAGCCTGTTGGTGAAGTTATCGATTTGTATACGAAAAATGGTTTGTCGTTTCCCGTTGAGGGGGTAAGCCCGGATTGACG
GAGAGAGGAAGCGTGTCGAG

Downstream 100 bases:

>100_bases
CCGGAGTCCATGAGCCGTCTGCTACGCTTCCACGGGGGCGGGGCGCCGTGAGAGGTGTCCCGCCCCTCGTGCGTTCAGTG
TCGTTAGGCCGCGAAGCTGT

Product: folD bifunctional protein

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MNLLDGKKCAESLVAEIARKVAGYTESGLRTPHMTIILVGEHAPSESYVKSKIATSGLAGFESTLLRFPETIRESELLAK
IKEVNEDPTTDGLIVQLPLPKHINQQHVINAIAPEKDIDGFHPANFGRMTLGQKAFRPATAYGICKLLQFYEVPVKAKHC
VVIGRSNIVGKPISIMLSNDFDIGNATVTLTHIETPRELLLDETRRADIVIVAVGIPGFVTADMVKDGVVLIDVGINRLE
SGKIVGDVDFENVAQKCSWITPVPGGVGRMTVAALMINTLMAYQNNFNLA

Sequences:

>Translated_290_residues
MNLLDGKKCAESLVAEIARKVAGYTESGLRTPHMTIILVGEHAPSESYVKSKIATSGLAGFESTLLRFPETIRESELLAK
IKEVNEDPTTDGLIVQLPLPKHINQQHVINAIAPEKDIDGFHPANFGRMTLGQKAFRPATAYGICKLLQFYEVPVKAKHC
VVIGRSNIVGKPISIMLSNDFDIGNATVTLTHIETPRELLLDETRRADIVIVAVGIPGFVTADMVKDGVVLIDVGINRLE
SGKIVGDVDFENVAQKCSWITPVPGGVGRMTVAALMINTLMAYQNNFNLA
>Mature_290_residues
MNLLDGKKCAESLVAEIARKVAGYTESGLRTPHMTIILVGEHAPSESYVKSKIATSGLAGFESTLLRFPETIRESELLAK
IKEVNEDPTTDGLIVQLPLPKHINQQHVINAIAPEKDIDGFHPANFGRMTLGQKAFRPATAYGICKLLQFYEVPVKAKHC
VVIGRSNIVGKPISIMLSNDFDIGNATVTLTHIETPRELLLDETRRADIVIVAVGIPGFVTADMVKDGVVLIDVGINRLE
SGKIVGDVDFENVAQKCSWITPVPGGVGRMTVAALMINTLMAYQNNFNLA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=45.945945945946, Blast_Score=254, Evalue=9e-68,
Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=44.5205479452055, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI222136639, Length=291, Percent_Identity=39.1752577319588, Blast_Score=184, Evalue=5e-47,
Organism=Escherichia coli, GI1786741, Length=280, Percent_Identity=47.1428571428571, Blast_Score=243, Evalue=9e-66,
Organism=Caenorhabditis elegans, GI17568735, Length=292, Percent_Identity=37.6712328767123, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6319558, Length=298, Percent_Identity=41.6107382550336, Blast_Score=212, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6321643, Length=300, Percent_Identity=40.3333333333333, Blast_Score=186, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6322933, Length=319, Percent_Identity=24.1379310344828, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI17136818, Length=296, Percent_Identity=42.9054054054054, Blast_Score=240, Evalue=7e-64,
Organism=Drosophila melanogaster, GI17136816, Length=296, Percent_Identity=42.9054054054054, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=37.3702422145329, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=37.3702422145329, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=37.3702422145329, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=37.3702422145329, Blast_Score=178, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD1_GEOSL (Q74GN1)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951276.1
- HSSP:   P11586
- ProteinModelPortal:   Q74GN1
- SMR:   Q74GN1
- GeneID:   2687787
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0215
- NMPDR:   fig|243231.1.peg.214
- TIGR:   GSU0215
- HOGENOM:   HBG328751
- OMA:   GFHPTNF
- ProtClustDB:   CLSK743076
- BioCyc:   GSUL243231:GSU_0215-MONOMER
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31486; Mature: 31486

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLDGKKCAESLVAEIARKVAGYTESGLRTPHMTIILVGEHAPSESYVKSKIATSGLAG
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCHH
FESTLLRFPETIRESELLAKIKEVNEDPTTDGLIVQLPLPKHINQQHVINAIAPEKDIDG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCC
FHPANFGRMTLGQKAFRPATAYGICKLLQFYEVPVKAKHCVVIGRSNIVGKPISIMLSND
CCCCCCCCHHHCHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEECC
FDIGNATVTLTHIETPRELLLDETRRADIVIVAVGIPGFVTADMVKDGVVLIDVGINRLE
CCCCCEEEEEEECCCCHHHHHHCCCCCCEEEEEECCCCCEEHHHHHCCEEEEEECCCCCC
SGKIVGDVDFENVAQKCSWITPVPGGVGRMTVAALMINTLMAYQNNFNLA
CCCEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNLLDGKKCAESLVAEIARKVAGYTESGLRTPHMTIILVGEHAPSESYVKSKIATSGLAG
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCHH
FESTLLRFPETIRESELLAKIKEVNEDPTTDGLIVQLPLPKHINQQHVINAIAPEKDIDG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCC
FHPANFGRMTLGQKAFRPATAYGICKLLQFYEVPVKAKHCVVIGRSNIVGKPISIMLSND
CCCCCCCCHHHCHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEECC
FDIGNATVTLTHIETPRELLLDETRRADIVIVAVGIPGFVTADMVKDGVVLIDVGINRLE
CCCCCEEEEEEECCCCHHHHHHCCCCCCEEEEEECCCCCEEHHHHHCCEEEEEECCCCCC
SGKIVGDVDFENVAQKCSWITPVPGGVGRMTVAALMINTLMAYQNNFNLA
CCCEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA