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The map label for this gene is dnaN [H]

Identifier: 39995112

GI number: 39995112

Start: 1594

End: 2712

Strand: Direct

Name: dnaN [H]

Synonym: GSU0001

Alternate gene names: 39995112

Gene position: 1594-2712 (Clockwise)

Preceding gene: 47525234

Following gene: 39995113

Centisome position: 0.04

GC content: 56.39

Gene sequence:

>1119_bases
ATGGAATTCACGATTGACAGGGACACCTTCTCGCGGGCTCTCCAGAAGATCCAGGGGATCGTCGAAAAGCGCAACACCAT
GCCGATCCTCTCCAACGTCCTCATCGAGGCACTGGAAGATCGGATCGAGCTGACCGCCACCGACCTGGAAGTCGGCATGA
AGAGCTCCTACCCCACAACGGTTGCGAGCCAGGGCAAAATCACCGTATCGGCCAAGAAGCTCTATGAAATAGTCAAGGAG
CTTCCCGACGAGACCATATCATTTCTGACCAAAGCGAACGACTATGTGGAGATCCGCTGCGGCAAGGCCAAGTTCACTAT
TGTCGGCCTTTCCTCCGAGGAGTTCCCCTATTTCCCCAAGGTCAATGAAGAGAGCTTCATCAGGATCGAAAGCGGCCTGC
TGGCCGACATGATCGAGAAAACCTCGTATGCCATCTGCTTCGATGAGACCAAGTACAATCTGAACGGTACCTTCGTGAAG
GCGTCCGAGGAAGATGGCCGCTCGATACTTCGCATGGTTGCCACCGACGGCCACCGGCTCTCCATCACCCAGAGGGAATT
CAACGGTGCAGTAAGCCCCGAGATGGCCAAGGGGGTCATCTTCCCCAAGAAAGGCATCTTCGAGCTGAAAAAGATGTGCG
AGGAGGAGTCGACCCAGCTTTCCCTGGGCTTCCTGGATAACAGCGCCGTGATCGTTAAGGGAAACACCGTCGTGGTGATG
CGCCTGGTGGACGGAGAGTTTCCCGATTACACCAGGGTGGTGCCGGTGGCCAATGACCGGATCGTCACCGTGGCGCGGGA
TCCGTTCTTCCATTCGCTGCGCAGGATGTCCATCCTTTCCAGCGAAAAGTTCAAGGGCATCAAGATGGATATTCAGGAAA
GCGGCATGGTGATCTCTTCCAGCAACCCGGAACTGGGCGAGGCGTCCGAAGAACTGGACGCCGTATTTGCCGGAGATGCC
ATCTCCATCCGCTTCAATGCCAAGTACCTCATCGATGTGCTGGCAGTGCTGGATGAGAGCAGCGTGGCACTCCACCTGAA
GGACGAGCTTTCTCCGGCCATCGTGAGGCCGGCTGACGGCGACGGTTTCACAGCGGTAATTATGCCCATGCGTCTGTAG

Upstream 100 bases:

>100_bases
CGTTGGGCGATTATCCCCATATCCACAGCCCCTACTGTTTCTACTGATGTTTTAAGAAAACATAACCATAACTATGATGA
CCTGTTGAAAAGGAGGAGCG

Downstream 100 bases:

>100_bases
GAGAAGGTATTTCCGAGACGAACAGGCAAAGGCCGCTCCCCGGGAGAGGCCTTTTGCCGTTTCTTGCAGGCCGGTCATTC
ATGCACCTGAACGCAATTGC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MEFTIDRDTFSRALQKIQGIVEKRNTMPILSNVLIEALEDRIELTATDLEVGMKSSYPTTVASQGKITVSAKKLYEIVKE
LPDETISFLTKANDYVEIRCGKAKFTIVGLSSEEFPYFPKVNEESFIRIESGLLADMIEKTSYAICFDETKYNLNGTFVK
ASEEDGRSILRMVATDGHRLSITQREFNGAVSPEMAKGVIFPKKGIFELKKMCEEESTQLSLGFLDNSAVIVKGNTVVVM
RLVDGEFPDYTRVVPVANDRIVTVARDPFFHSLRRMSILSSEKFKGIKMDIQESGMVISSSNPELGEASEELDAVFAGDA
ISIRFNAKYLIDVLAVLDESSVALHLKDELSPAIVRPADGDGFTAVIMPMRL

Sequences:

>Translated_372_residues
MEFTIDRDTFSRALQKIQGIVEKRNTMPILSNVLIEALEDRIELTATDLEVGMKSSYPTTVASQGKITVSAKKLYEIVKE
LPDETISFLTKANDYVEIRCGKAKFTIVGLSSEEFPYFPKVNEESFIRIESGLLADMIEKTSYAICFDETKYNLNGTFVK
ASEEDGRSILRMVATDGHRLSITQREFNGAVSPEMAKGVIFPKKGIFELKKMCEEESTQLSLGFLDNSAVIVKGNTVVVM
RLVDGEFPDYTRVVPVANDRIVTVARDPFFHSLRRMSILSSEKFKGIKMDIQESGMVISSSNPELGEASEELDAVFAGDA
ISIRFNAKYLIDVLAVLDESSVALHLKDELSPAIVRPADGDGFTAVIMPMRL
>Mature_372_residues
MEFTIDRDTFSRALQKIQGIVEKRNTMPILSNVLIEALEDRIELTATDLEVGMKSSYPTTVASQGKITVSAKKLYEIVKE
LPDETISFLTKANDYVEIRCGKAKFTIVGLSSEEFPYFPKVNEESFIRIESGLLADMIEKTSYAICFDETKYNLNGTFVK
ASEEDGRSILRMVATDGHRLSITQREFNGAVSPEMAKGVIFPKKGIFELKKMCEEESTQLSLGFLDNSAVIVKGNTVVVM
RLVDGEFPDYTRVVPVANDRIVTVARDPFFHSLRRMSILSSEKFKGIKMDIQESGMVISSSNPELGEASEELDAVFAGDA
ISIRFNAKYLIDVLAVLDESSVALHLKDELSPAIVRPADGDGFTAVIMPMRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=373, Percent_Identity=31.0991957104558, Blast_Score=206, Evalue=2e-54,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41332; Mature: 41332

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFTIDRDTFSRALQKIQGIVEKRNTMPILSNVLIEALEDRIELTATDLEVGMKSSYPTT
CCEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEHHHHCCCCCCCCE
VASQGKITVSAKKLYEIVKELPDETISFLTKANDYVEIRCGKAKFTIVGLSSEEFPYFPK
ECCCCEEEEEHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCEEEEEEECCCCCCCCCCC
VNEESFIRIESGLLADMIEKTSYAICFDETKYNLNGTFVKASEEDGRSILRMVATDGHRL
CCCCCEEEECCCHHHHHHHHCCEEEEEECCEECCCEEEEECCCHHHHHHHHHHHCCCCEE
SITQREFNGAVSPEMAKGVIFPKKGIFELKKMCEEESTQLSLGFLDNSAVIVKGNTVVVM
EEEHHHCCCCCCCHHHCCEECCCCCHHHHHHHHHCCCCEEEEEEECCCEEEEECCEEEEE
RLVDGEFPDYTRVVPVANDRIVTVARDPFFHSLRRMSILSSEKFKGIKMDIQESGMVISS
EEECCCCCCCEEEEEECCCEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEHHHCCEEEEC
SNPELGEASEELDAVFAGDAISIRFNAKYLIDVLAVLDESSVALHLKDELSPAIVRPADG
CCCCCCCCHHHHHEEEECCEEEEEECHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCC
DGFTAVIMPMRL
CCEEEEEEEECC
>Mature Secondary Structure
MEFTIDRDTFSRALQKIQGIVEKRNTMPILSNVLIEALEDRIELTATDLEVGMKSSYPTT
CCEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEHHHHCCCCCCCCE
VASQGKITVSAKKLYEIVKELPDETISFLTKANDYVEIRCGKAKFTIVGLSSEEFPYFPK
ECCCCEEEEEHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCEEEEEEECCCCCCCCCCC
VNEESFIRIESGLLADMIEKTSYAICFDETKYNLNGTFVKASEEDGRSILRMVATDGHRL
CCCCCEEEECCCHHHHHHHHCCEEEEEECCEECCCEEEEECCCHHHHHHHHHHHCCCCEE
SITQREFNGAVSPEMAKGVIFPKKGIFELKKMCEEESTQLSLGFLDNSAVIVKGNTVVVM
EEEHHHCCCCCCCHHHCCEECCCCCHHHHHHHHHCCCCEEEEEEECCCEEEEECCEEEEE
RLVDGEFPDYTRVVPVANDRIVTVARDPFFHSLRRMSILSSEKFKGIKMDIQESGMVISS
EEECCCCCCCEEEEEECCCEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEHHHCCEEEEC
SNPELGEASEELDAVFAGDAISIRFNAKYLIDVLAVLDESSVALHLKDELSPAIVRPADG
CCCCCCCCHHHHHEEEECCEEEEEECHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCC
DGFTAVIMPMRL
CCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]