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The map label for this gene is recF

Identifier: 39995113

GI number: 39995113

Start: 2793

End: 3890

Strand: Direct

Name: recF

Synonym: GSU0002

Alternate gene names: 39995113

Gene position: 2793-3890 (Clockwise)

Preceding gene: 39995112

Following gene: 39995114

Centisome position: 0.07

GC content: 61.29

Gene sequence:

>1098_bases
ATGCACCTGAACGCAATTGCCATCAGCGCCTTCCGCAATATCGATCATGTGGAAATCTCATTCGATCGGCGGTTCAACGT
CCTGCACGGCGCCAATGGACAGGGAAAAACGTCGGTGTTGGAGGCCATTTACCTCCTGGGGACCATGAAATCGTTCCGGA
TGGCCAAGGCCCATGATCTCATCGCCTGGAACGCCCCCCACTCACTGGTACGTGGCGATATCGACAAGGCGGGGGTCAGA
CGTGAGATAGCCCTCTATCTCGGCCGCGAGGGCCGTAAGGCCCGCATTGACCGGAAACCGGTCACCAAGCTTGCGGATTT
TTTCGGCGCCGTGAACGCCGTGGTATTTTCTCCAGAGGAGATAGGCATGGCCCGCGGAGGGCCAGAACTCAGACGCCGCT
ACCTGGACCGGGCCATCTTCAACGGCGACTTGGGGTACCTGCTCCTCCACCATGAGTACCATCGGCTGCTCAAGCAGCGT
AATGCTCTGCTGAGGCGGGGCGAACGGGATGGATTGGAGGTCTGGACAATCCAGCTGGCCGAGGCCGGCGCCCGGCTCAT
GGTAAAGCGAAGGGCCTACCTTTCTCAGATAGAGCCGCTGGTACGGCAGTTTTACCGGGATATTGCCGGTGCGGGCCAGG
AGGTGGGACTTGCCTATCGCTGCCACGGACTGGCATCCGCCGAGGGCGAACGCGATTGTGCAGCGGCCCTCCGGGAGCTC
ATGGCAGCCCATGAGGCCGAGGAACTGCGCCGGGGGGCCACTGGCGTAGGCCCCCACCGGGATGACGTGGATTTCATCCT
GAACGGTCGGGTCATCCGCCACCACGGCTCCCAAGGGGAGCAGCGGAGCTTTGTCCTGGCCGTAAAAATGGCCGAGATCG
AGTACCTGGAGCGGCTCAACGGTGCGCCGCCGGTGCTTCTGCTGGACGACATCTCATCGGAACTGGATCCCGAGCGCAAC
GCGAACCTGATGACCTTTCTCCGGGGAAAACGGATGCAGGTCTTCATAACCACCACCGATGTATCAACCCTCCGTCTGGA
GGGGATCGACACCCACGCGTCGTTTCGCGTTTCACGTGGAACGGTGACGCCTGTATAG

Upstream 100 bases:

>100_bases
TTATGCCCATGCGTCTGTAGGAGAAGGTATTTCCGAGACGAACAGGCAAAGGCCGCTCCCCGGGAGAGGCCTTTTGCCGT
TTCTTGCAGGCCGGTCATTC

Downstream 100 bases:

>100_bases
AGGGAGAGGCATGACCGACGAACAGAACTACGATTACGGTGCAGACAAGATCAAGGTCCTGGAGGGGCTCGAGGCGGTAC
GCAAGCGCCCCGCCATGTAC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MHLNAIAISAFRNIDHVEISFDRRFNVLHGANGQGKTSVLEAIYLLGTMKSFRMAKAHDLIAWNAPHSLVRGDIDKAGVR
REIALYLGREGRKARIDRKPVTKLADFFGAVNAVVFSPEEIGMARGGPELRRRYLDRAIFNGDLGYLLLHHEYHRLLKQR
NALLRRGERDGLEVWTIQLAEAGARLMVKRRAYLSQIEPLVRQFYRDIAGAGQEVGLAYRCHGLASAEGERDCAAALREL
MAAHEAEELRRGATGVGPHRDDVDFILNGRVIRHHGSQGEQRSFVLAVKMAEIEYLERLNGAPPVLLLDDISSELDPERN
ANLMTFLRGKRMQVFITTTDVSTLRLEGIDTHASFRVSRGTVTPV

Sequences:

>Translated_365_residues
MHLNAIAISAFRNIDHVEISFDRRFNVLHGANGQGKTSVLEAIYLLGTMKSFRMAKAHDLIAWNAPHSLVRGDIDKAGVR
REIALYLGREGRKARIDRKPVTKLADFFGAVNAVVFSPEEIGMARGGPELRRRYLDRAIFNGDLGYLLLHHEYHRLLKQR
NALLRRGERDGLEVWTIQLAEAGARLMVKRRAYLSQIEPLVRQFYRDIAGAGQEVGLAYRCHGLASAEGERDCAAALREL
MAAHEAEELRRGATGVGPHRDDVDFILNGRVIRHHGSQGEQRSFVLAVKMAEIEYLERLNGAPPVLLLDDISSELDPERN
ANLMTFLRGKRMQVFITTTDVSTLRLEGIDTHASFRVSRGTVTPV
>Mature_365_residues
MHLNAIAISAFRNIDHVEISFDRRFNVLHGANGQGKTSVLEAIYLLGTMKSFRMAKAHDLIAWNAPHSLVRGDIDKAGVR
REIALYLGREGRKARIDRKPVTKLADFFGAVNAVVFSPEEIGMARGGPELRRRYLDRAIFNGDLGYLLLHHEYHRLLKQR
NALLRRGERDGLEVWTIQLAEAGARLMVKRRAYLSQIEPLVRQFYRDIAGAGQEVGLAYRCHGLASAEGERDCAAALREL
MAAHEAEELRRGATGVGPHRDDVDFILNGRVIRHHGSQGEQRSFVLAVKMAEIEYLERLNGAPPVLLLDDISSELDPERN
ANLMTFLRGKRMQVFITTTDVSTLRLEGIDTHASFRVSRGTVTPV

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=367, Percent_Identity=28.8828337874659, Blast_Score=135, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_GEOSL (Q74H90)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951064.1
- ProteinModelPortal:   Q74H90
- GeneID:   2686552
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0002
- NMPDR:   fig|243231.1.peg.2
- TIGR:   GSU0002
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   CLSK827576
- BioCyc:   GSUL243231:GSU_0002-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 40963; Mature: 40963

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLNAIAISAFRNIDHVEISFDRRFNVLHGANGQGKTSVLEAIYLLGTMKSFRMAKAHDL
CCCHHEEHHHHCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCE
IAWNAPHSLVRGDIDKAGVRREIALYLGREGRKARIDRKPVTKLADFFGAVNAVVFSPEE
EEECCCHHHHHCCCHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHEEECHHH
IGMARGGPELRRRYLDRAIFNGDLGYLLLHHEYHRLLKQRNALLRRGERDGLEVWTIQLA
HCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEH
EAGARLMVKRRAYLSQIEPLVRQFYRDIAGAGQEVGLAYRCHGLASAEGERDCAAALREL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHH
MAAHEAEELRRGATGVGPHRDDVDFILNGRVIRHHGSQGEQRSFVLAVKMAEIEYLERLN
HHHHHHHHHHCCCCCCCCCCCCCEEEECCEEEEECCCCCCCCEEEEEEEHHHHHHHHHCC
GAPPVLLLDDISSELDPERNANLMTFLRGKRMQVFITTTDVSTLRLEGIDTHASFRVSRG
CCCCEEEEECCHHHCCCCCCCCEEEEECCCEEEEEEEECCCEEEEEECCCCCCEEEEECC
TVTPV
CCCCC
>Mature Secondary Structure
MHLNAIAISAFRNIDHVEISFDRRFNVLHGANGQGKTSVLEAIYLLGTMKSFRMAKAHDL
CCCHHEEHHHHCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCE
IAWNAPHSLVRGDIDKAGVRREIALYLGREGRKARIDRKPVTKLADFFGAVNAVVFSPEE
EEECCCHHHHHCCCHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHEEECHHH
IGMARGGPELRRRYLDRAIFNGDLGYLLLHHEYHRLLKQRNALLRRGERDGLEVWTIQLA
HCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEH
EAGARLMVKRRAYLSQIEPLVRQFYRDIAGAGQEVGLAYRCHGLASAEGERDCAAALREL
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHH
MAAHEAEELRRGATGVGPHRDDVDFILNGRVIRHHGSQGEQRSFVLAVKMAEIEYLERLN
HHHHHHHHHHCCCCCCCCCCCCCEEEECCEEEEECCCCCCCCEEEEEEEHHHHHHHHHCC
GAPPVLLLDDISSELDPERNANLMTFLRGKRMQVFITTTDVSTLRLEGIDTHASFRVSRG
CCCCEEEEECCHHHCCCCCCCCEEEEECCCEEEEEEEECCCEEEEEECCCCCCEEEEECC
TVTPV
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA