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The map label for this gene is ubiE [H]

Identifier: 57234789

GI number: 57234789

Start: 378628

End: 379395

Strand: Direct

Name: ubiE [H]

Synonym: DET0399

Alternate gene names: 57234789

Gene position: 378628-379395 (Clockwise)

Preceding gene: 57234797

Following gene: 57234788

Centisome position: 25.76

GC content: 48.05

Gene sequence:

>768_bases
ATGAGCAGTGTGAATGTAAGCGTAAAAGACCCCGGTTTTGAGTCTGAAGAGGCTCAGGCCCGCAAGCGTTATATGCTCCA
GCTGTTTGGCTTTCAGGCCAAACACTATGATTTGCACGATGATATTATAGGTATGGGTATTCACCGCCGCTGGGGCAAAG
AGATGGTTTTAGAGATACTGACCTATACCCGCAATATGCCCCAGGTGAAAATGCTGGATTTGGCCTGCGGGACCGGTTTT
GTTACCTTTAACACTCTGCGTTACATGAATAATGTGGATATAGACGCCTTTGATATTACCCCCGAAATGGTGGAAGTAGC
CAAGGGGCGGCTCAAGAAAAACTACCCTGACCGCAATGTAAAGTTCTGGGTGGGTGATGCCGAGGTACCCTACGGGGATG
CCAAGTACGATGTTATCGGCACCTGCTTTGCCTTCCGCAATTTTGCCAACAAAAATCTGGCGGCCTCTAATATTTTTAAA
GCACTTAAACCGGGCGGTCTGCTGGTTTTGCAGGATATGACCAAACCCGAAAAACAGCCCTTCCGCGGTATTTACCTGTT
TGCCTTGAAAAACTTGATGCCCATCATGGCTCGGATAATAGGCACAGAACGCAAATCAGCCCGGTATCTGGCAAATTCGG
TGCTGGCCATGCCCAGTAACGCGGAAATAATGTCTATCCTCCAGAATCAGGGTTTTATAAATATCAAATCCCGCTATCAG
AGCGGTGGTATGGGTACACTGACCGTGGCCTATAAGCCGGAGAATTAA

Upstream 100 bases:

>100_bases
CTGATGGAAAGTAAAGGCTTTGAGAAATGCCAGTACAAGAGCCTGTCACTGGGTATTGCCTGCGTAATCTGGGGTTTCAA
GCCGGAGAAATCAGAATAAG

Downstream 100 bases:

>100_bases
AATGGGTAAAGTGGATACACTGGCTTATGACATTGGGAATAACCGTACCCGCCACGTATATATAGGCAGTGACATACTGG
GTAGTCTGCCCGAATATGCA

Product: ubiquinone/menaquinone biosynthesis methlytransferase UbiE, putative

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSSVNVSVKDPGFESEEAQARKRYMLQLFGFQAKHYDLHDDIIGMGIHRRWGKEMVLEILTYTRNMPQVKMLDLACGTGF
VTFNTLRYMNNVDIDAFDITPEMVEVAKGRLKKNYPDRNVKFWVGDAEVPYGDAKYDVIGTCFAFRNFANKNLAASNIFK
ALKPGGLLVLQDMTKPEKQPFRGIYLFALKNLMPIMARIIGTERKSARYLANSVLAMPSNAEIMSILQNQGFINIKSRYQ
SGGMGTLTVAYKPEN

Sequences:

>Translated_255_residues
MSSVNVSVKDPGFESEEAQARKRYMLQLFGFQAKHYDLHDDIIGMGIHRRWGKEMVLEILTYTRNMPQVKMLDLACGTGF
VTFNTLRYMNNVDIDAFDITPEMVEVAKGRLKKNYPDRNVKFWVGDAEVPYGDAKYDVIGTCFAFRNFANKNLAASNIFK
ALKPGGLLVLQDMTKPEKQPFRGIYLFALKNLMPIMARIIGTERKSARYLANSVLAMPSNAEIMSILQNQGFINIKSRYQ
SGGMGTLTVAYKPEN
>Mature_254_residues
SSVNVSVKDPGFESEEAQARKRYMLQLFGFQAKHYDLHDDIIGMGIHRRWGKEMVLEILTYTRNMPQVKMLDLACGTGFV
TFNTLRYMNNVDIDAFDITPEMVEVAKGRLKKNYPDRNVKFWVGDAEVPYGDAKYDVIGTCFAFRNFANKNLAASNIFKA
LKPGGLLVLQDMTKPEKQPFRGIYLFALKNLMPIMARIIGTERKSARYLANSVLAMPSNAEIMSILQNQGFINIKSRYQS
GGMGTLTVAYKPEN

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=280, Percent_Identity=27.5, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI48994983, Length=243, Percent_Identity=26.7489711934156, Blast_Score=101, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17557039, Length=247, Percent_Identity=27.9352226720648, Blast_Score=111, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6323526, Length=243, Percent_Identity=24.6913580246914, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24641702, Length=256, Percent_Identity=24.21875, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28878; Mature: 28747

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVNVSVKDPGFESEEAQARKRYMLQLFGFQAKHYDLHDDIIGMGIHRRWGKEMVLEIL
CCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHH
TYTRNMPQVKMLDLACGTGFVTFNTLRYMNNVDIDAFDITPEMVEVAKGRLKKNYPDRNV
HHHCCCCCEEEEEEECCCCEEEHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCCC
KFWVGDAEVPYGDAKYDVIGTCFAFRNFANKNLAASNIFKALKPGGLLVLQDMTKPEKQP
EEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCC
FRGIYLFALKNLMPIMARIIGTERKSARYLANSVLAMPSNAEIMSILQNQGFINIKSRYQ
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCC
SGGMGTLTVAYKPEN
CCCCEEEEEEECCCC
>Mature Secondary Structure 
SSVNVSVKDPGFESEEAQARKRYMLQLFGFQAKHYDLHDDIIGMGIHRRWGKEMVLEIL
CCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHH
TYTRNMPQVKMLDLACGTGFVTFNTLRYMNNVDIDAFDITPEMVEVAKGRLKKNYPDRNV
HHHCCCCCEEEEEEECCCCEEEHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCCC
KFWVGDAEVPYGDAKYDVIGTCFAFRNFANKNLAASNIFKALKPGGLLVLQDMTKPEKQP
EEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCC
FRGIYLFALKNLMPIMARIIGTERKSARYLANSVLAMPSNAEIMSILQNQGFINIKSRYQ
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCC
SGGMGTLTVAYKPEN
CCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA