Click here to switch to the map view.

The map label for this gene is ubiE [H]

Identifier: 57234797

GI number: 57234797

Start: 377850

End: 378626

Strand: Direct

Name: ubiE [H]

Synonym: DET0398

Alternate gene names: 57234797

Gene position: 377850-378626 (Clockwise)

Preceding gene: 57234802

Following gene: 57234789

Centisome position: 25.71

GC content: 48.01

Gene sequence:

>777_bases
ATGAGTAGTTTGGCCGAAGCGCCTATCCATGACCCCGGATTTGACTCTGAAGAGGCGCATAAACGTAAACAGTATATGGT
TGACTTGTTCGTAGTCCAAGCAAAAAACTACGATTTCCATGATGATGTTTTCGGGCTTTATGCCCACCGTTTCTGGATGC
GGACCGTTCTTAAGATAATGGGGCGGTTTCTGAAAGACCGGAAGCATGTTGATATGCTGGATTTGGCCTGCGGCACCGGC
TTTGTTACCTTCAATACAGCCAGAAACTTTGACAATGTTGACATAGAGGCCTTTGATATCAGCCCGGATATGCTGGCCGT
TGCTAAAGAACGCTACGAAAAGTATTTCAAAGGGCGGAACATCAAGTTCTGGCGGGGTGATGCCGAAGTTCCCTTTGGCG
AAGAGAAATATGACCTGGTAACTACCAGTTTTGCTTACCGCAACTTTGCCAACAAAGGCATTGCCACCGAAAACGTTTTC
AATGCCCTGAAGCCGGGCGGTCTGTTTATTATTCAGGACCTTACCAAACCCGAACGCCACCCCATGCGCGGGCTGTATAT
ATTCTACATGAAGTATATCCTGCCGGTGCTTACCGGCATTCTGGGCACCGAAAAATCCGCTGCCCGCTGGCTTTACAAGT
CTGCCATGATGATGCCCACTAACGGGCAGATTGTAAAACTGATGGAAAGTAAAGGCTTTGAGAAATGCCAGTACAAGAGC
CTGTCACTGGGTATTGCCTGCGTAATCTGGGGTTTCAAGCCGGAGAAATCAGAATAA

Upstream 100 bases:

>100_bases
CTTACTCAAAAAAGCTGAGTTTTGTCATTGGTGGGTTCCCCGTTTCAATGACTTCAGATATGGAATATGTCTAAATAAAT
GCTTCAAAAGGTGATAGCTG

Downstream 100 bases:

>100_bases
GATGAGCAGTGTGAATGTAAGCGTAAAAGACCCCGGTTTTGAGTCTGAAGAGGCTCAGGCCCGCAAGCGTTATATGCTCC
AGCTGTTTGGCTTTCAGGCC

Product: ubiquinone/menaquinone biosynthesis methlytransferase UbiE, putative

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSSLAEAPIHDPGFDSEEAHKRKQYMVDLFVVQAKNYDFHDDVFGLYAHRFWMRTVLKIMGRFLKDRKHVDMLDLACGTG
FVTFNTARNFDNVDIEAFDISPDMLAVAKERYEKYFKGRNIKFWRGDAEVPFGEEKYDLVTTSFAYRNFANKGIATENVF
NALKPGGLFIIQDLTKPERHPMRGLYIFYMKYILPVLTGILGTEKSAARWLYKSAMMMPTNGQIVKLMESKGFEKCQYKS
LSLGIACVIWGFKPEKSE

Sequences:

>Translated_258_residues
MSSLAEAPIHDPGFDSEEAHKRKQYMVDLFVVQAKNYDFHDDVFGLYAHRFWMRTVLKIMGRFLKDRKHVDMLDLACGTG
FVTFNTARNFDNVDIEAFDISPDMLAVAKERYEKYFKGRNIKFWRGDAEVPFGEEKYDLVTTSFAYRNFANKGIATENVF
NALKPGGLFIIQDLTKPERHPMRGLYIFYMKYILPVLTGILGTEKSAARWLYKSAMMMPTNGQIVKLMESKGFEKCQYKS
LSLGIACVIWGFKPEKSE
>Mature_257_residues
SSLAEAPIHDPGFDSEEAHKRKQYMVDLFVVQAKNYDFHDDVFGLYAHRFWMRTVLKIMGRFLKDRKHVDMLDLACGTGF
VTFNTARNFDNVDIEAFDISPDMLAVAKERYEKYFKGRNIKFWRGDAEVPFGEEKYDLVTTSFAYRNFANKGIATENVFN
ALKPGGLFIIQDLTKPERHPMRGLYIFYMKYILPVLTGILGTEKSAARWLYKSAMMMPTNGQIVKLMESKGFEKCQYKSL
SLGIACVIWGFKPEKSE

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=278, Percent_Identity=26.978417266187, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI48994983, Length=243, Percent_Identity=27.1604938271605, Blast_Score=112, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17557039, Length=250, Percent_Identity=27.6, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323526, Length=269, Percent_Identity=28.6245353159851, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24641702, Length=256, Percent_Identity=25.390625, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29792; Mature: 29661

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLAEAPIHDPGFDSEEAHKRKQYMVDLFVVQAKNYDFHDDVFGLYAHRFWMRTVLKIM
CCCCCCCCCCCCCCCCHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
GRFLKDRKHVDMLDLACGTGFVTFNTARNFDNVDIEAFDISPDMLAVAKERYEKYFKGRN
HHHHHCCCCCCHHHHHCCCCEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCC
IKFWRGDAEVPFGEEKYDLVTTSFAYRNFANKGIATENVFNALKPGGLFIIQDLTKPERH
EEEEECCCCCCCCCCCHHHEEHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEECCCCCCCC
PMRGLYIFYMKYILPVLTGILGTEKSAARWLYKSAMMMPTNGQIVKLMESKGFEKCQYKS
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCCCEEEEEHHCCCCHHHHHH
LSLGIACVIWGFKPEKSE
HCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
SSLAEAPIHDPGFDSEEAHKRKQYMVDLFVVQAKNYDFHDDVFGLYAHRFWMRTVLKIM
CCCCCCCCCCCCCCCHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
GRFLKDRKHVDMLDLACGTGFVTFNTARNFDNVDIEAFDISPDMLAVAKERYEKYFKGRN
HHHHHCCCCCCHHHHHCCCCEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCC
IKFWRGDAEVPFGEEKYDLVTTSFAYRNFANKGIATENVFNALKPGGLFIIQDLTKPERH
EEEEECCCCCCCCCCCHHHEEHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEECCCCCCCC
PMRGLYIFYMKYILPVLTGILGTEKSAARWLYKSAMMMPTNGQIVKLMESKGFEKCQYKS
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCCCEEEEEHHCCCCHHHHHH
LSLGIACVIWGFKPEKSE
HCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA