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The map label for this gene is cobS-2 [H]

Identifier: 57234514

GI number: 57234514

Start: 637858

End: 638622

Strand: Direct

Name: cobS-2 [H]

Synonym: DET0692

Alternate gene names: 57234514

Gene position: 637858-638622 (Clockwise)

Preceding gene: 57234522

Following gene: 57234513

Centisome position: 43.4

GC content: 52.29

Gene sequence:

>765_bases
ATGGGTTCATTCTTAGCGGCTTTCCGCTTTCTTACCAATATACCTGTACCCTGGCTGAAAGAAGACTGGCAGGGGGAGAA
GTCACATCTGGATTTTGCCCGCTCACTTGGCTTCTACCCCCTGGTGGGTTTAATTGTCGGGTTGATACTGGCCGTTTTGG
GCTGGGTTTTCAGCCTTTTTTTACCGGATGTACTTACGGCCGCTTTGCTGACAGTAAGCCTGGTGGTGATTACCGGCGGT
TTGCATCTTGACGGCCTGACAGATACCTTTGACGGTATTGCCGCCGGCCACAAGTCCTTTGAACGCGCCCAGGAGGTAAT
GCATTTGCCGGGGGTGGGGGCTATCGGGGTACTTGCGGCAATCTGCCTGTTACTTTCAAAGTTTGCCGCTATCGTTAGCC
TTCCTTCAGGCAGTTTCATAACCGGGCTTATCCTGTTTCCTGTAATCAGCCGCTGGGCTATGGTCTATGCGGTGGTTTGT
TACCCGTATGTCAGGTCACAGGGTTTGGGCAAAGAGCTTAAGGGCGGGTCTGCAAAAGCCAGCTTGTGGCTTGCTACCAT
TTTCTGTCTGGCAGTCTGTGTAATACTGGGTGGCTGGGCAGGGTTGCTGGCTATGTTCGGCAGCTGGCTGCTGATTATGT
ATCTGGCATCTTTCTTCAGACGCAGGCTTGGCGGACTAAACGGGGATACTTACGGCTCAATTAACGAGTTTACCGAGGTA
GCGGCGCTGATATTTATAGTGGCCTTGTCCGGGTATTTCAGCTGA

Upstream 100 bases:

>100_bases
AACCGTATCCAGCACGAGATGGCCTCTTTTGCAGATGCCGGGGTTTCCGAAAAGCAAAGTTAGGTGTATTCTGGTATAAT
CCCGGTTTGAGGAAATATAT

Downstream 100 bases:

>100_bases
AATAGAGGGAGTTTATAGCATGAAACTGATACTGGTGCGTCACGGGGAGACCGAAACAGATAACTGCCGCTGTTACTGGG
GGCATAGTGATATCGGCCTT

Product: cobalamin 5'-phosphate synthase

Products: Cobalamin 5'-Phosphate; GMP; Cobalamin [C]

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MGSFLAAFRFLTNIPVPWLKEDWQGEKSHLDFARSLGFYPLVGLIVGLILAVLGWVFSLFLPDVLTAALLTVSLVVITGG
LHLDGLTDTFDGIAAGHKSFERAQEVMHLPGVGAIGVLAAICLLLSKFAAIVSLPSGSFITGLILFPVISRWAMVYAVVC
YPYVRSQGLGKELKGGSAKASLWLATIFCLAVCVILGGWAGLLAMFGSWLLIMYLASFFRRRLGGLNGDTYGSINEFTEV
AALIFIVALSGYFS

Sequences:

>Translated_254_residues
MGSFLAAFRFLTNIPVPWLKEDWQGEKSHLDFARSLGFYPLVGLIVGLILAVLGWVFSLFLPDVLTAALLTVSLVVITGG
LHLDGLTDTFDGIAAGHKSFERAQEVMHLPGVGAIGVLAAICLLLSKFAAIVSLPSGSFITGLILFPVISRWAMVYAVVC
YPYVRSQGLGKELKGGSAKASLWLATIFCLAVCVILGGWAGLLAMFGSWLLIMYLASFFRRRLGGLNGDTYGSINEFTEV
AALIFIVALSGYFS
>Mature_253_residues
GSFLAAFRFLTNIPVPWLKEDWQGEKSHLDFARSLGFYPLVGLIVGLILAVLGWVFSLFLPDVLTAALLTVSLVVITGGL
HLDGLTDTFDGIAAGHKSFERAQEVMHLPGVGAIGVLAAICLLLSKFAAIVSLPSGSFITGLILFPVISRWAMVYAVVCY
PYVRSQGLGKELKGGSAKASLWLATIFCLAVCVILGGWAGLLAMFGSWLLIMYLASFFRRRLGGLNGDTYGSINEFTEVA
ALIFIVALSGYFS

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=252, Percent_Identity=32.1428571428571, Blast_Score=98, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 27263; Mature: 27131

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCC
>Mature Secondary Structure 
GSFLAAFRFLTNIPVPWLKEDWQGEKSHLDFARSLGFYPLVGLIVGLILAVLGWVFSLF
CHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LPDVLTAALLTVSLVVITGGLHLDGLTDTFDGIAAGHKSFERAQEVMHLPGVGAIGVLAA
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ICLLLSKFAAIVSLPSGSFITGLILFPVISRWAMVYAVVCYPYVRSQGLGKELKGGSAKA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHH
SLWLATIFCLAVCVILGGWAGLLAMFGSWLLIMYLASFFRRRLGGLNGDTYGSINEFTEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
AALIFIVALSGYFS
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-Cobinamide; Alpha-Ribazole 5'-Phosphate; Alpha-Ribazole [C]

Specific reaction: GDP-Cobinamide + Alpha-Ribazole 5'-Phosphate (or Alpha-Ribazole) = Cobalamin 5'-Phosphate (or GDP-Cobalamin) + GMP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA