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The map label for this gene is nuoE2 [H]

Identifier: 57234358

GI number: 57234358

Start: 791186

End: 791671

Strand: Direct

Name: nuoE2 [H]

Synonym: DET0863

Alternate gene names: 57234358

Gene position: 791186-791671 (Clockwise)

Preceding gene: 57234359

Following gene: 57234357

Centisome position: 53.83

GC content: 45.68

Gene sequence:

>486_bases
ATGTGTAATTGTGAAACCAAAGCACCTGAGGTTATAGACCTGTCAAAGCTGGATAATATCCTGGACGGTTACCGTGATAA
AGCTGATATGCTTATTCAGGTACTCCTTAAAATCCAAAAAGAATATAACTGGCTTCCCAAAGAGGCCTTGTATAAAGTCA
GCCAAGCTCTTAACGTACCGGTAAACCGGGTCTATCATGTGGCCACTTTTTATAAACTTTTCAGCGTGATTCCCAAAGGT
AAGCACAATGTGAGTGTTTGCGTAGGTACTGCCTGCCATGTGTTTGGGGCACCCAAAATACTGGACAGGCTGGAAAAATC
ACTGGGTATCAAAGCCGGTGAAACTACGCCTGACCTTAAGTTTTCCCTGGAAACAGTAAACTGTCTGGGTTGCTGCGCCT
TAGGGCCGGTGGTGGTGGTTGACGGTCACTATCATGGCAAACTGCCCACTGCTGACGCCGAAAAAATACTGGCAGATTAC
GATTAG

Upstream 100 bases:

>100_bases
GCCAGACCGGAGGCTATTATAGACGGCGTGGTTATGGGGCTGGGCAAGCTGGCTGAGAAAAAAGCCAAGGCCAAAACTGA
AGCCAAACGTGAGGAACAGC

Downstream 100 bases:

>100_bases
TTAGGAGCAGATGATGCCAAGGCTGAATACTCCGGCTGAGCTGGAAACCCTGCGGAAAAAACTGGTCAAAAACCAGTCTG
CCTCCCGCCCCTGCATAACG

Product: hydrogenase subunit HymA, putative

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E 2; NDH-1 subunit E 2 [H]

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MCNCETKAPEVIDLSKLDNILDGYRDKADMLIQVLLKIQKEYNWLPKEALYKVSQALNVPVNRVYHVATFYKLFSVIPKG
KHNVSVCVGTACHVFGAPKILDRLEKSLGIKAGETTPDLKFSLETVNCLGCCALGPVVVVDGHYHGKLPTADAEKILADY
D

Sequences:

>Translated_161_residues
MCNCETKAPEVIDLSKLDNILDGYRDKADMLIQVLLKIQKEYNWLPKEALYKVSQALNVPVNRVYHVATFYKLFSVIPKG
KHNVSVCVGTACHVFGAPKILDRLEKSLGIKAGETTPDLKFSLETVNCLGCCALGPVVVVDGHYHGKLPTADAEKILADY
D
>Mature_161_residues
MCNCETKAPEVIDLSKLDNILDGYRDKADMLIQVLLKIQKEYNWLPKEALYKVSQALNVPVNRVYHVATFYKLFSVIPKG
KHNVSVCVGTACHVFGAPKILDRLEKSLGIKAGETTPDLKFSLETVNCLGCCALGPVVVVDGHYHGKLPTADAEKILADY
D

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=147, Percent_Identity=39.4557823129252, Blast_Score=113, Evalue=6e-26,
Organism=Escherichia coli, GI1788621, Length=144, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17561328, Length=147, Percent_Identity=40.8163265306122, Blast_Score=113, Evalue=5e-26,
Organism=Drosophila melanogaster, GI18859877, Length=146, Percent_Identity=39.7260273972603, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21355353, Length=129, Percent_Identity=32.5581395348837, Blast_Score=88, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17778; Mature: 17778

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCNCETKAPEVIDLSKLDNILDGYRDKADMLIQVLLKIQKEYNWLPKEALYKVSQALNVP
CCCCCCCCCCEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
VNRVYHVATFYKLFSVIPKGKHNVSVCVGTACHVFGAPKILDRLEKSLGIKAGETTPDLK
HHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCE
FSLETVNCLGCCALGPVVVVDGHYHGKLPTADAEKILADYD
EEHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MCNCETKAPEVIDLSKLDNILDGYRDKADMLIQVLLKIQKEYNWLPKEALYKVSQALNVP
CCCCCCCCCCEECHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
VNRVYHVATFYKLFSVIPKGKHNVSVCVGTACHVFGAPKILDRLEKSLGIKAGETTPDLK
HHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCE
FSLETVNCLGCCALGPVVVVDGHYHGKLPTADAEKILADYD
EEHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]