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The map label for this gene is nuoF2 [H]

Identifier: 57234357

GI number: 57234357

Start: 791685

End: 793607

Strand: Direct

Name: nuoF2 [H]

Synonym: DET0864

Alternate gene names: 57234357

Gene position: 791685-793607 (Clockwise)

Preceding gene: 57234358

Following gene: 57234356

Centisome position: 53.87

GC content: 50.03

Gene sequence:

>1923_bases
ATGCCAAGGCTGAATACTCCGGCTGAGCTGGAAACCCTGCGGAAAAAACTGGTCAAAAACCAGTCTGCCTCCCGCCCCTG
CATAACGGTATGCTGCGGTACAGGATGCCGGGCATTGGGTTCGGTTAAACTGGTAGATGCTTTCCGGGCCGAGCTTGCCA
AGCAGGGCCTGGAAAGTTCAGTTGATATTAAAGAAACCGGCTGCCATGGTTTTTGTGAAAAAGGTTCGGTGGTTGTTATC
TATCCCCAAAATATCTGCTATTTCCATGTAAAACCTGAAGACGCCGCAGACGTTGTCGCTAAAACAATAAAAACCGGCGA
ACTGGTAGACCATTTGCTTTACAAAGACCCTGCCAGCGGCGAAAAGCTGGTTTATCATCAGGATATTCCCTTTTACAAGT
ACCAGGAACGGACTGTACTGGGCGATAACGAGCATATAAATCCCCAGAATATAGAGGATTATATTCAGCGGGGCGGTTAT
TCCGCCCTGGTTAAAGCCCTTTTCCAGATGACCCCGGAATCTGTACTGGAAGAAGTAGATACAGCCAATCTGCGCGGACG
CGGCGGCGACGGTTTCCCTGCCGGTAAAAAGTGGCGCACCACCCGTGATGCTCCTGACCCGGTCAAATACGTGCTGGTTA
ACTGTGACGAGGGTGACCCAGGGGCATTTATGGACCGCTCCATCATGGAGGGTAACCCCCACTGCGTGCTGGAAGGTCTG
GCTATAGGGGCTTTTGCAGTCGGCGCCAAAGAGGGTTATATATATGTACGTGCCGAATACCCATTGGCGGTGGAAAACCT
GTACGCTGCCATAAGGCAGGCAGAGGAGTATGGTCTGCTGGGCAAAAATATACTTGGCTCCGGATTTGATTTTATTGTCA
AAGTCCACGAAGGAGCCGGGGCTTTTGTATCCGGCGAATCGTCCGCACTGATGACTGCTATTGAAGGGCGGGTTGGTGAA
CCCCGCCCCAAATATATCCGCACTGCGATCAAGGGGCTCTGGGATAAACCCTCAAATCTAAATAACGTGGAGACCTGGGC
TAACGTACCCCATATTATAAACAAAGGGGCGGAGTGGTTCAGGTCTATCGGTACCGAAGGCAGCAAAGGCACCAAAATAT
TTGCTTTGGTTGGCAAGGTTAACAATACCGGACTGGTAGAAGTACCTATGGGTACTACCCTCCGGGATATTATATTTAAA
ATAGGCGGCGGCCTGCGTGACGGCAAAAAAATAAAAGCTGTCCAGACAGGCGGCCCCTCAGGCGGCTGCATACCCGAAAA
CCTGCTGGATTCGCCTGTTGATTATGATGTGCTGAGTAAACTGGGTTCTATGGTAGGTTCAGGCGGGCTTATCATAATGG
ACGAAGAAACCTGCATGGTAGATATTGCCCGCTATTTTATAAACTTTTTGTCAGATGAATCCTGCGGCAAATGCCTGCCC
TGCCGCGAGGGACTCCGCCAGCTGGTAGAAATACTTACCCGTATTACCGAAGGCAAAGGCACTATGGCAGATATGGACAC
TCTGGAAGACCTGAGCGGGGTAATGAGTGAAGCCTGCCTGTGCGCTTTGGGACAGCAAGCCCCAAATCCCCTGCTGACCA
CCCTTAAATATTTCCGTCACGAATACATTGACCATATTAAAAACAAACACTGTGAAGCCGGCGTCTGCAAAGCCTTGCTT
ACTTTCTATATTGACCCTGACAAATGCAAAGCCTGCATGATTTGCGCCCGAAACTGCCCTACTGATGCCATAAAGGGCGG
CAAAGGGCTTATTCATACTATAGAACAGGATAAATGCATCAAGTGCGGAGCCTGTCTGGATACCTGCCCGGCCAAATTTT
ATGCGGTCAGGAAAATATCCGGCATACCTATACCGGACAACGTACCGTCTGAACCTATTCCTTTAAACCGGAAGGCAGAA
TAA

Upstream 100 bases:

>100_bases
TTAGGGCCGGTGGTGGTGGTTGACGGTCACTATCATGGCAAACTGCCCACTGCTGACGCCGAAAAAATACTGGCAGATTA
CGATTAGTTAGGAGCAGATG

Downstream 100 bases:

>100_bases
TGGAAGAAATCAAGCTTACCATAGATGGCAGGGAAGTTACTGCCCAAAGCGGGCAAACTGTTTTTCAGGCAGCGGCGGCA
GCCGGTATTCATATACCCGC

Product: hydrogenase subunit HymB, putative

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 640; Mature: 639

Protein sequence:

>640_residues
MPRLNTPAELETLRKKLVKNQSASRPCITVCCGTGCRALGSVKLVDAFRAELAKQGLESSVDIKETGCHGFCEKGSVVVI
YPQNICYFHVKPEDAADVVAKTIKTGELVDHLLYKDPASGEKLVYHQDIPFYKYQERTVLGDNEHINPQNIEDYIQRGGY
SALVKALFQMTPESVLEEVDTANLRGRGGDGFPAGKKWRTTRDAPDPVKYVLVNCDEGDPGAFMDRSIMEGNPHCVLEGL
AIGAFAVGAKEGYIYVRAEYPLAVENLYAAIRQAEEYGLLGKNILGSGFDFIVKVHEGAGAFVSGESSALMTAIEGRVGE
PRPKYIRTAIKGLWDKPSNLNNVETWANVPHIINKGAEWFRSIGTEGSKGTKIFALVGKVNNTGLVEVPMGTTLRDIIFK
IGGGLRDGKKIKAVQTGGPSGGCIPENLLDSPVDYDVLSKLGSMVGSGGLIIMDEETCMVDIARYFINFLSDESCGKCLP
CREGLRQLVEILTRITEGKGTMADMDTLEDLSGVMSEACLCALGQQAPNPLLTTLKYFRHEYIDHIKNKHCEAGVCKALL
TFYIDPDKCKACMICARNCPTDAIKGGKGLIHTIEQDKCIKCGACLDTCPAKFYAVRKISGIPIPDNVPSEPIPLNRKAE

Sequences:

>Translated_640_residues
MPRLNTPAELETLRKKLVKNQSASRPCITVCCGTGCRALGSVKLVDAFRAELAKQGLESSVDIKETGCHGFCEKGSVVVI
YPQNICYFHVKPEDAADVVAKTIKTGELVDHLLYKDPASGEKLVYHQDIPFYKYQERTVLGDNEHINPQNIEDYIQRGGY
SALVKALFQMTPESVLEEVDTANLRGRGGDGFPAGKKWRTTRDAPDPVKYVLVNCDEGDPGAFMDRSIMEGNPHCVLEGL
AIGAFAVGAKEGYIYVRAEYPLAVENLYAAIRQAEEYGLLGKNILGSGFDFIVKVHEGAGAFVSGESSALMTAIEGRVGE
PRPKYIRTAIKGLWDKPSNLNNVETWANVPHIINKGAEWFRSIGTEGSKGTKIFALVGKVNNTGLVEVPMGTTLRDIIFK
IGGGLRDGKKIKAVQTGGPSGGCIPENLLDSPVDYDVLSKLGSMVGSGGLIIMDEETCMVDIARYFINFLSDESCGKCLP
CREGLRQLVEILTRITEGKGTMADMDTLEDLSGVMSEACLCALGQQAPNPLLTTLKYFRHEYIDHIKNKHCEAGVCKALL
TFYIDPDKCKACMICARNCPTDAIKGGKGLIHTIEQDKCIKCGACLDTCPAKFYAVRKISGIPIPDNVPSEPIPLNRKAE
>Mature_639_residues
PRLNTPAELETLRKKLVKNQSASRPCITVCCGTGCRALGSVKLVDAFRAELAKQGLESSVDIKETGCHGFCEKGSVVVIY
PQNICYFHVKPEDAADVVAKTIKTGELVDHLLYKDPASGEKLVYHQDIPFYKYQERTVLGDNEHINPQNIEDYIQRGGYS
ALVKALFQMTPESVLEEVDTANLRGRGGDGFPAGKKWRTTRDAPDPVKYVLVNCDEGDPGAFMDRSIMEGNPHCVLEGLA
IGAFAVGAKEGYIYVRAEYPLAVENLYAAIRQAEEYGLLGKNILGSGFDFIVKVHEGAGAFVSGESSALMTAIEGRVGEP
RPKYIRTAIKGLWDKPSNLNNVETWANVPHIINKGAEWFRSIGTEGSKGTKIFALVGKVNNTGLVEVPMGTTLRDIIFKI
GGGLRDGKKIKAVQTGGPSGGCIPENLLDSPVDYDVLSKLGSMVGSGGLIIMDEETCMVDIARYFINFLSDESCGKCLPC
REGLRQLVEILTRITEGKGTMADMDTLEDLSGVMSEACLCALGQQAPNPLLTTLKYFRHEYIDHIKNKHCEAGVCKALLT
FYIDPDKCKACMICARNCPTDAIKGGKGLIHTIEQDKCIKCGACLDTCPAKFYAVRKISGIPIPDNVPSEPIPLNRKAE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=402, Percent_Identity=38.3084577114428, Blast_Score=272, Evalue=6e-73,
Organism=Homo sapiens, GI20149568, Length=402, Percent_Identity=38.3084577114428, Blast_Score=272, Evalue=7e-73,
Organism=Escherichia coli, GI1788620, Length=420, Percent_Identity=41.9047619047619, Blast_Score=328, Evalue=8e-91,
Organism=Caenorhabditis elegans, GI17531727, Length=431, Percent_Identity=37.8190255220418, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI19920794, Length=390, Percent_Identity=37.9487179487179, Blast_Score=264, Evalue=2e-70,
Organism=Drosophila melanogaster, GI45550452, Length=381, Percent_Identity=39.1076115485564, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24653819, Length=374, Percent_Identity=33.6898395721925, Blast_Score=203, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24653817, Length=335, Percent_Identity=32.5373134328358, Blast_Score=174, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 69677; Mature: 69546

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00645 COMPLEX1_51K_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLNTPAELETLRKKLVKNQSASRPCITVCCGTGCRALGSVKLVDAFRAELAKQGLESS
CCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHCCHHHHHHHHHHHHHHCCCCC
VDIKETGCHGFCEKGSVVVIYPQNICYFHVKPEDAADVVAKTIKTGELVDHLLYKDPASG
CCHHHCCCCCCCCCCCEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
EKLVYHQDIPFYKYQERTVLGDNEHINPQNIEDYIQRGGYSALVKALFQMTPESVLEEVD
CEEEEECCCCCEEECCCEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCHHHHHHHHC
TANLRGRGGDGFPAGKKWRTTRDAPDPVKYVLVNCDEGDPGAFMDRSIMEGNPHCVLEGL
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHCCHHHCCCCCEEEEHH
AIGAFAVGAKEGYIYVRAEYPLAVENLYAAIRQAEEYGLLGKNILGSGFDFIVKVHEGAG
HHHHEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEEEECCCC
AFVSGESSALMTAIEGRVGEPRPKYIRTAIKGLWDKPSNLNNVETWANVPHIINKGAEWF
CEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHH
RSIGTEGSKGTKIFALVGKVNNTGLVEVPMGTTLRDIIFKIGGGLRDGKKIKAVQTGGPS
HHHCCCCCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCC
GGCIPENLLDSPVDYDVLSKLGSMVGSGGLIIMDEETCMVDIARYFINFLSDESCGKCLP
CCCCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCH
CREGLRQLVEILTRITEGKGTMADMDTLEDLSGVMSEACLCALGQQAPNPLLTTLKYFRH
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EYIDHIKNKHCEAGVCKALLTFYIDPDKCKACMICARNCPTDAIKGGKGLIHTIEQDKCI
HHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHCCCCCEEEECCCCCCE
KCGACLDTCPAKFYAVRKISGIPIPDNVPSEPIPLNRKAE
ECCCHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PRLNTPAELETLRKKLVKNQSASRPCITVCCGTGCRALGSVKLVDAFRAELAKQGLESS
CCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHCCHHHHHHHHHHHHHHCCCCC
VDIKETGCHGFCEKGSVVVIYPQNICYFHVKPEDAADVVAKTIKTGELVDHLLYKDPASG
CCHHHCCCCCCCCCCCEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
EKLVYHQDIPFYKYQERTVLGDNEHINPQNIEDYIQRGGYSALVKALFQMTPESVLEEVD
CEEEEECCCCCEEECCCEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCHHHHHHHHC
TANLRGRGGDGFPAGKKWRTTRDAPDPVKYVLVNCDEGDPGAFMDRSIMEGNPHCVLEGL
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHCCHHHCCCCCEEEEHH
AIGAFAVGAKEGYIYVRAEYPLAVENLYAAIRQAEEYGLLGKNILGSGFDFIVKVHEGAG
HHHHEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEEEECCCC
AFVSGESSALMTAIEGRVGEPRPKYIRTAIKGLWDKPSNLNNVETWANVPHIINKGAEWF
CEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHH
RSIGTEGSKGTKIFALVGKVNNTGLVEVPMGTTLRDIIFKIGGGLRDGKKIKAVQTGGPS
HHHCCCCCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCC
GGCIPENLLDSPVDYDVLSKLGSMVGSGGLIIMDEETCMVDIARYFINFLSDESCGKCLP
CCCCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCH
CREGLRQLVEILTRITEGKGTMADMDTLEDLSGVMSEACLCALGQQAPNPLLTTLKYFRH
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EYIDHIKNKHCEAGVCKALLTFYIDPDKCKACMICARNCPTDAIKGGKGLIHTIEQDKCI
HHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHCCCCCEEEECCCCCCE
KCGACLDTCPAKFYAVRKISGIPIPDNVPSEPIPLNRKAE
ECCCHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]