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The map label for this gene is yeiT [H]

Identifier: 57234355

GI number: 57234355

Start: 794203

End: 795588

Strand: Direct

Name: yeiT [H]

Synonym: DET0866

Alternate gene names: 57234355

Gene position: 794203-795588 (Clockwise)

Preceding gene: 57234356

Following gene: 57234354

Centisome position: 54.04

GC content: 49.21

Gene sequence:

>1386_bases
ATGTTAGAAAATGCTGAATATATAAAAGCCGAGGAACTGCTTGACCAGACCCAGAAGCTTTATGACGAGGGGGCGATATT
CTGTACCGCCTCCTGCGTAGACTTGGGTAACGAATTTGAAGTTATTTACCACTACAATCTGGAAAATGGTCTTCAGATGA
AACACCTCCGGCTTAAAATAGATAAAAATGAAACTGTTCCCAGTATTTCAAACATATATCTATGTGCTTCGCTCATTGAA
AACGAAATGCAGGAGCTTTACCAGCTCAAACTCAGCAAAATTGCCATAGATTTCAGCGGCGGGTTTCTGGTCACCAAAGA
AACCCCCAAGAGCTATATGATAAAAGCCCCTGATTACAAGCTTATTCCGGTAGAACGCCTGACTGCCCCCTGCCAGAGGG
CTTGCCCGGCAGGTATAGATGTATCCCGTTATGTCCGCCTTTGCGGCGAAGGGAACTATGATGCGGCCCTGGCTGTAATC
AAACAGGCCATGCCTTTCCCCGGAATACTGGGGCGGGTTTGCTTAGCCCCCTGTGAATCTGCCTGCCGCCAGGGCAAGTG
CGGAGAAGCTATAAGCATCAAACAGCTTAAACGGGCGGCCTATGAATACGGCCATTATACAGATACCGCCACCGCCAAAC
CTACCGGCAAAAAAGTGGCAGTGGTTGGCTCTGGTCCGGCTGGTCTGGCAGCAGCTTATTTTCTGACCAAAAAGGGGCAT
AAGGTAACTGTGTTTGAGGCTTTGCCGAAAGCCGGCGGCTATATGCGGGTAGGCATACCTGAGTATGCACTGCCCCGCCA
GATACTGGATGCCGAAATTGAAAACGTTGCCAAACTGGGTGTTGAGTTCAAACTGGGTACAGCCGTAAATTCGCTGAGTA
GCCTCAAAGAAATGGGGTTTGATGCCACTTTGCTGGCGCTGGGTGCCAATCAGGGGGTACGCCGCTCCAACATAATAGCC
GCTTTCAGCGGTGCTACAGACGTATTTAAAAAATTCGGGCTGGCTGTTGAAAATATAAACGGCAGCAATGTGCTTAAGGT
TGACGATGATACGCTTAGTACTTCGCAGGAGGGTGTTTTTGCCTGCGGAGACGCCGTAAACGGGCCTACTTCGGTAATTC
ATGCCGTTGCCTCCGGTAAAAAAGCCGCCGCCAGTATAGACAAATATCTGGGCAGTGCCGGCAAGTGGGTGTATGAAAAT
ATTGTAGCCCACGAGCCAGTATCCCGTGATACTTTCCTTGAACGGATTTTTCCCAAGAGCAAACCTTTAAGTGTTAAATA
TGATATTAAGCAAGCCAAAGAGCGAAATGAAGAAACTGCCGGCTATAGCCGTGAAGTTGCCGCCGCCGAGGGCAAACGCT
GCTGGCGCTGTGATTTGGAGGAATAA

Upstream 100 bases:

>100_bases
GCGAAAAATGCCAGCAGTGCTTTGATATCTGCCCCACCAATTTTATCCGGGCTAATTTTGAAATGGCCAAAGCTCTCACT
TTCGGGACTGAGGAATAGTT

Downstream 100 bases:

>100_bases
ATGGCACGGACAGTAATACCCTTCGGGCCACAGCATCCGGTATTCCCTGAGCCGGTACAGCTTAAACTGGAATGCGAAGA
CGAAACGGTAGTCGGGGTTA

Product: hydrogenase subunit, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MLENAEYIKAEELLDQTQKLYDEGAIFCTASCVDLGNEFEVIYHYNLENGLQMKHLRLKIDKNETVPSISNIYLCASLIE
NEMQELYQLKLSKIAIDFSGGFLVTKETPKSYMIKAPDYKLIPVERLTAPCQRACPAGIDVSRYVRLCGEGNYDAALAVI
KQAMPFPGILGRVCLAPCESACRQGKCGEAISIKQLKRAAYEYGHYTDTATAKPTGKKVAVVGSGPAGLAAAYFLTKKGH
KVTVFEALPKAGGYMRVGIPEYALPRQILDAEIENVAKLGVEFKLGTAVNSLSSLKEMGFDATLLALGANQGVRRSNIIA
AFSGATDVFKKFGLAVENINGSNVLKVDDDTLSTSQEGVFACGDAVNGPTSVIHAVASGKKAAASIDKYLGSAGKWVYEN
IVAHEPVSRDTFLERIFPKSKPLSVKYDIKQAKERNEETAGYSREVAAAEGKRCWRCDLEE

Sequences:

>Translated_461_residues
MLENAEYIKAEELLDQTQKLYDEGAIFCTASCVDLGNEFEVIYHYNLENGLQMKHLRLKIDKNETVPSISNIYLCASLIE
NEMQELYQLKLSKIAIDFSGGFLVTKETPKSYMIKAPDYKLIPVERLTAPCQRACPAGIDVSRYVRLCGEGNYDAALAVI
KQAMPFPGILGRVCLAPCESACRQGKCGEAISIKQLKRAAYEYGHYTDTATAKPTGKKVAVVGSGPAGLAAAYFLTKKGH
KVTVFEALPKAGGYMRVGIPEYALPRQILDAEIENVAKLGVEFKLGTAVNSLSSLKEMGFDATLLALGANQGVRRSNIIA
AFSGATDVFKKFGLAVENINGSNVLKVDDDTLSTSQEGVFACGDAVNGPTSVIHAVASGKKAAASIDKYLGSAGKWVYEN
IVAHEPVSRDTFLERIFPKSKPLSVKYDIKQAKERNEETAGYSREVAAAEGKRCWRCDLEE
>Mature_461_residues
MLENAEYIKAEELLDQTQKLYDEGAIFCTASCVDLGNEFEVIYHYNLENGLQMKHLRLKIDKNETVPSISNIYLCASLIE
NEMQELYQLKLSKIAIDFSGGFLVTKETPKSYMIKAPDYKLIPVERLTAPCQRACPAGIDVSRYVRLCGEGNYDAALAVI
KQAMPFPGILGRVCLAPCESACRQGKCGEAISIKQLKRAAYEYGHYTDTATAKPTGKKVAVVGSGPAGLAAAYFLTKKGH
KVTVFEALPKAGGYMRVGIPEYALPRQILDAEIENVAKLGVEFKLGTAVNSLSSLKEMGFDATLLALGANQGVRRSNIIA
AFSGATDVFKKFGLAVENINGSNVLKVDDDTLSTSQEGVFACGDAVNGPTSVIHAVASGKKAAASIDKYLGSAGKWVYEN
IVAHEPVSRDTFLERIFPKSKPLSVKYDIKQAKERNEETAGYSREVAAAEGKRCWRCDLEE

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=224, Percent_Identity=27.6785714285714, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1788468, Length=191, Percent_Identity=33.5078534031414, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1789606, Length=192, Percent_Identity=34.375, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789243, Length=194, Percent_Identity=38.659793814433, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788811, Length=193, Percent_Identity=33.160621761658, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87082180, Length=198, Percent_Identity=31.8181818181818, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17570289, Length=165, Percent_Identity=36.3636363636364, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71984108, Length=201, Percent_Identity=28.3582089552239, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=257, Percent_Identity=28.7937743190661, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28574881, Length=184, Percent_Identity=36.4130434782609, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24665539, Length=184, Percent_Identity=36.4130434782609, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24665547, Length=184, Percent_Identity=36.4130434782609, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24665543, Length=184, Percent_Identity=36.4130434782609, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24640763, Length=197, Percent_Identity=32.48730964467, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI18858217, Length=197, Percent_Identity=32.48730964467, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 50162; Mature: 50162

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENAEYIKAEELLDQTQKLYDEGAIFCTASCVDLGNEFEVIYHYNLENGLQMKHLRLKI
CCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCEEEEEEEECCCCCEEEEEEEEE
DKNETVPSISNIYLCASLIENEMQELYQLKLSKIAIDFSGGFLVTKETPKSYMIKAPDYK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEECCCCCEEEECCCCE
LIPVERLTAPCQRACPAGIDVSRYVRLCGEGNYDAALAVIKQAMPFPGILGRVCLAPCES
EEEHHHHCCCHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ACRQGKCGEAISIKQLKRAAYEYGHYTDTATAKPTGKKVAVVGSGPAGLAAAYFLTKKGH
HHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCC
KVTVFEALPKAGGYMRVGIPEYALPRQILDAEIENVAKLGVEFKLGTAVNSLSSLKEMGF
EEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCC
DATLLALGANQGVRRSNIIAAFSGATDVFKKFGLAVENINGSNVLKVDDDTLSTSQEGVF
CEEEEEECCCCCCCCCCEEEEECCHHHHHHHHCCEEECCCCCCEEEECCCCCCCCCCCEE
ACGDAVNGPTSVIHAVASGKKAAASIDKYLGSAGKWVYENIVAHEPVSRDTFLERIFPKS
EECCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHCCCC
KPLSVKYDIKQAKERNEETAGYSREVAAAEGKRCWRCDLEE
CCCEEEECHHHHHHCCHHHCCCCHHHHHHCCCCEEECCCCC
>Mature Secondary Structure
MLENAEYIKAEELLDQTQKLYDEGAIFCTASCVDLGNEFEVIYHYNLENGLQMKHLRLKI
CCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCEEEEEEEECCCCCEEEEEEEEE
DKNETVPSISNIYLCASLIENEMQELYQLKLSKIAIDFSGGFLVTKETPKSYMIKAPDYK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEECCCCCEEEECCCCE
LIPVERLTAPCQRACPAGIDVSRYVRLCGEGNYDAALAVIKQAMPFPGILGRVCLAPCES
EEEHHHHCCCHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ACRQGKCGEAISIKQLKRAAYEYGHYTDTATAKPTGKKVAVVGSGPAGLAAAYFLTKKGH
HHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCC
KVTVFEALPKAGGYMRVGIPEYALPRQILDAEIENVAKLGVEFKLGTAVNSLSSLKEMGF
EEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCC
DATLLALGANQGVRRSNIIAAFSGATDVFKKFGLAVENINGSNVLKVDDDTLSTSQEGVF
CEEEEEECCCCCCCCCCEEEEECCHHHHHHHHCCEEECCCCCCEEEECCCCCCCCCCCEE
ACGDAVNGPTSVIHAVASGKKAAASIDKYLGSAGKWVYENIVAHEPVSRDTFLERIFPKS
EECCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHCCCC
KPLSVKYDIKQAKERNEETAGYSREVAAAEGKRCWRCDLEE
CCCEEEECHHHHHHCCHHHCCCCHHHHHHCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]