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The map label for this gene is cooH [H]

Identifier: 57234354

GI number: 57234354

Start: 795589

End: 796668

Strand: Direct

Name: cooH [H]

Synonym: DET0867

Alternate gene names: 57234354

Gene position: 795589-796668 (Clockwise)

Preceding gene: 57234355

Following gene: 57234353

Centisome position: 54.13

GC content: 52.87

Gene sequence:

>1080_bases
ATGGCACGGACAGTAATACCCTTCGGGCCACAGCATCCGGTATTCCCTGAGCCGGTACAGCTTAAACTGGAATGCGAAGA
CGAAACGGTAGTCGGGGTTACGCCGGTTATCGGTTACGTACACCGCGGGGTAGAAAAGGCCTGTGAGATAAATACCTTGC
GGCAGAATATTTTTCTGGTAGAACGTATTTGCGGCATCTGTTCAATTCAGCACGCCCTTTGCTATTGTCAGGGCATAGAG
GAACTGGCCGGTATTGAAGTTCCCCAACGGGCACATTATCTGCGCACTGCCTGGGCTGAGCTTTCCCGTCTTCACAGCCA
TAGCCTCTGGCTGGGTTTGCTGGCGGATGCACTCGGTTTTGAAAGCCTGTTCATGCAAATCTGGCGCGCCCGTGAGATTA
TCCTGGATATACTGGAGATGACTGCCGGACACAGGGTTATTACTTCTGCCTGCGTGGTGGGCGGAGTGCGCCGTGACATA
GACACCGAAATGCTGAAAAAAACGTCTGATATGCTGGCTGAGTTTAGAAAAATGATGGATAAAACTATCCTGCCTACCCT
GCTGAATGACCCCACCTTGAAAAAACGGACTGTAGGCAAAGGTATTTTATCCTATGACCAGGCGGTTACCTTAGGTGCTT
GCGGCCCTACCATGCGCGGCAGCGGCGTCCCATCTGATGCCCGGGCCACCGGGTATGCCGCTTTTAAGGAGCTTGGCTTT
GAGCCGGTAGTGGAAACCGCCGGGGACAGCTATGCCCGCACCCTGGTGCGCACCCGTGAAATTTACCAATCCATAGACCT
TACGCTGGCCGCGCTTCAGCAAATGCCAGCAGGGGAAATAATGGTTAAGGCCGAAGCCTATCCTGAGGGAGAAATATTTA
CCCGGGTGGAGCAGCCCCGCGGCGAGCTTACTTATTATATAAAAGCCAACGGCACAAATCATTTGGACAGGTGCAAAATA
CGTACCCCCACCTTTGCCAATATCCCCACCCTGCTGGTCATGCTGCCGGGGTGTGAACTGGCAGATGTGCCTGTTATTAC
CTTATCTATAGACCCATGTATATCCTGCACGGAGCGTTAG

Upstream 100 bases:

>100_bases
TAAGCAAGCCAAAGAGCGAAATGAAGAAACTGCCGGCTATAGCCGTGAAGTTGCCGCCGCCGAGGGCAAACGCTGCTGGC
GCTGTGATTTGGAGGAATAA

Downstream 100 bases:

>100_bases
GAGAAGGCTATGCCCTGGATGATAGGAACAGTACTTAAAAATTTGTTTTCAACTCCCCCCACCCGCCGCTACCCATACGA
AAAACGGGAGTCATTTGCTG

Product: hydrogenase, group 4, EchE subunit, putative

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MARTVIPFGPQHPVFPEPVQLKLECEDETVVGVTPVIGYVHRGVEKACEINTLRQNIFLVERICGICSIQHALCYCQGIE
ELAGIEVPQRAHYLRTAWAELSRLHSHSLWLGLLADALGFESLFMQIWRAREIILDILEMTAGHRVITSACVVGGVRRDI
DTEMLKKTSDMLAEFRKMMDKTILPTLLNDPTLKKRTVGKGILSYDQAVTLGACGPTMRGSGVPSDARATGYAAFKELGF
EPVVETAGDSYARTLVRTREIYQSIDLTLAALQQMPAGEIMVKAEAYPEGEIFTRVEQPRGELTYYIKANGTNHLDRCKI
RTPTFANIPTLLVMLPGCELADVPVITLSIDPCISCTER

Sequences:

>Translated_359_residues
MARTVIPFGPQHPVFPEPVQLKLECEDETVVGVTPVIGYVHRGVEKACEINTLRQNIFLVERICGICSIQHALCYCQGIE
ELAGIEVPQRAHYLRTAWAELSRLHSHSLWLGLLADALGFESLFMQIWRAREIILDILEMTAGHRVITSACVVGGVRRDI
DTEMLKKTSDMLAEFRKMMDKTILPTLLNDPTLKKRTVGKGILSYDQAVTLGACGPTMRGSGVPSDARATGYAAFKELGF
EPVVETAGDSYARTLVRTREIYQSIDLTLAALQQMPAGEIMVKAEAYPEGEIFTRVEQPRGELTYYIKANGTNHLDRCKI
RTPTFANIPTLLVMLPGCELADVPVITLSIDPCISCTER
>Mature_358_residues
ARTVIPFGPQHPVFPEPVQLKLECEDETVVGVTPVIGYVHRGVEKACEINTLRQNIFLVERICGICSIQHALCYCQGIEE
LAGIEVPQRAHYLRTAWAELSRLHSHSLWLGLLADALGFESLFMQIWRAREIILDILEMTAGHRVITSACVVGGVRRDID
TEMLKKTSDMLAEFRKMMDKTILPTLLNDPTLKKRTVGKGILSYDQAVTLGACGPTMRGSGVPSDARATGYAAFKELGFE
PVVETAGDSYARTLVRTREIYQSIDLTLAALQQMPAGEIMVKAEAYPEGEIFTRVEQPRGELTYYIKANGTNHLDRCKIR
TPTFANIPTLLVMLPGCELADVPVITLSIDPCISCTER

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH) [H]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli formate hydrogenlyase hydrogenase isozyme 3 and to bovine mitochondrial NADH-ubiquinone oxidoreductase [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=381, Percent_Identity=29.1338582677165, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI260898743, Length=368, Percent_Identity=29.6195652173913, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1789076, Length=359, Percent_Identity=34.5403899721448, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI1788832, Length=357, Percent_Identity=35.5742296918768, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI145693162, Length=384, Percent_Identity=26.5625, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17568379, Length=388, Percent_Identity=28.3505154639175, Blast_Score=168, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17555284, Length=388, Percent_Identity=27.8350515463918, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI221459469, Length=376, Percent_Identity=30.0531914893617, Blast_Score=188, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24638644, Length=381, Percent_Identity=29.9212598425197, Blast_Score=177, Evalue=8e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001501 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 39794; Mature: 39663

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTVIPFGPQHPVFPEPVQLKLECEDETVVGVTPVIGYVHRGVEKACEINTLRQNIFLV
CCCCCCCCCCCCCCCCCCEEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERICGICSIQHALCYCQGIEELAGIEVPQRAHYLRTAWAELSRLHSHSLWLGLLADALGF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESLFMQIWRAREIILDILEMTAGHRVITSACVVGGVRRDIDTEMLKKTSDMLAEFRKMMD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTILPTLLNDPTLKKRTVGKGILSYDQAVTLGACGPTMRGSGVPSDARATGYAAFKELGF
HHHHHHHCCCCCHHHHHHHCHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCC
EPVVETAGDSYARTLVRTREIYQSIDLTLAALQQMPAGEIMVKAEAYPEGEIFTRVEQPR
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCCCCC
GELTYYIKANGTNHLDRCKIRTPTFANIPTLLVMLPGCELADVPVITLSIDPCISCTER
CCEEEEEEECCCCCCHHCEECCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure 
ARTVIPFGPQHPVFPEPVQLKLECEDETVVGVTPVIGYVHRGVEKACEINTLRQNIFLV
CCCCCCCCCCCCCCCCCEEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERICGICSIQHALCYCQGIEELAGIEVPQRAHYLRTAWAELSRLHSHSLWLGLLADALGF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESLFMQIWRAREIILDILEMTAGHRVITSACVVGGVRRDIDTEMLKKTSDMLAEFRKMMD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTILPTLLNDPTLKKRTVGKGILSYDQAVTLGACGPTMRGSGVPSDARATGYAAFKELGF
HHHHHHHCCCCCHHHHHHHCHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCC
EPVVETAGDSYARTLVRTREIYQSIDLTLAALQQMPAGEIMVKAEAYPEGEIFTRVEQPR
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCCCCC
GELTYYIKANGTNHLDRCKIRTPTFANIPTLLVMLPGCELADVPVITLSIDPCISCTER
CCEEEEEEECCCCCCHHCEECCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626276; 1644755 [H]