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The map label for this gene is hisF [H]

Identifier: 57234238

GI number: 57234238

Start: 878319

End: 879101

Strand: Direct

Name: hisF [H]

Synonym: DET0960

Alternate gene names: 57234238

Gene position: 878319-879101 (Clockwise)

Preceding gene: 57234240

Following gene: 57234237

Centisome position: 59.76

GC content: 48.02

Gene sequence:

>783_bases
GTGAACCCTATCAAGATTATCCCCTGTTTAGATGTGAAGAATAACCGGGTAACCAAAGCAATAGAGTTTACCCGAATGGC
AGATTCCTATGACCAGGCTGAAGCCGCTTTGGCATATTCCGGACAGGGGGCGGACGAACTGGCCTTTTTGGATATGACTG
CCATTCTGGAAGGAAGTGCTATCCACACGGACTGGGTTAAAAAGATTATAGGGAATACAGATTTGCCGCTGCTTGCGGGC
GGCGGTATCAGAGACCTGAGGGATATTAAAAGTTTGCTGTCTCTTGGTGTAAGCAGAGTTGCCCTAAATAACATTACACC
CCAAAATCTGGGTTTTCTGGCGGAAGCGGCAGCCAGATTCGGCAGTGAACGTATAATTACGGCTATAGATGGGATTAAAA
ACCCGCCCGAAAGCGGGCTTCACCGTCTGGAGGCTATTATGCCCGATACAGACAATAAACATGGTTTGGAACTGGTGAGC
TGGGTGAAACAGATTGAAAAGCAAGGAACAGGTGCTATTTTGCTTACCAGCCGGGATACTGACGGAACTAAAAGCGGCTA
TGACCTGGAGATGACCAAAGCAGTTGCCCAAGCAGTAAGTATACCGGTAATAGCTTCGGGCGGTGCCGGCAGTATGGAGG
ATATATATGCCGTAATAACCGGGGGCAAAGCCCAGGCGGTGCTTGCGGCTTCGGTATTTCATTCCGGCATGATAAATATA
ATGGAACTGAAAAAATATTTATACTCACGGGGTATACCGGTAAATATGCCGCCCCTCAGATAG

Upstream 100 bases:

>100_bases
ACACCTTTGAACCGCCAACTTTACTTTGAACCTCCAAATAGCATACTATTCCCAAGTGCGCCAGTTTACATGCAAATGAA
TAAAAACTGATGGTGATAAG

Downstream 100 bases:

>100_bases
TTTCCCGTAAAATACGCCTGCCGCTGTCCGGCCGAACAAATATAAAAATCTTGCCCTGCATACGGCAAATTATTGCCTTA
TATGACGGGGCGCTGTTACA

Product: imidazoleglycerol phosphate synthase, cyclase subunit, putative

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MNPIKIIPCLDVKNNRVTKAIEFTRMADSYDQAEAALAYSGQGADELAFLDMTAILEGSAIHTDWVKKIIGNTDLPLLAG
GGIRDLRDIKSLLSLGVSRVALNNITPQNLGFLAEAAARFGSERIITAIDGIKNPPESGLHRLEAIMPDTDNKHGLELVS
WVKQIEKQGTGAILLTSRDTDGTKSGYDLEMTKAVAQAVSIPVIASGGAGSMEDIYAVITGGKAQAVLAASVFHSGMINI
MELKKYLYSRGIPVNMPPLR

Sequences:

>Translated_260_residues
MNPIKIIPCLDVKNNRVTKAIEFTRMADSYDQAEAALAYSGQGADELAFLDMTAILEGSAIHTDWVKKIIGNTDLPLLAG
GGIRDLRDIKSLLSLGVSRVALNNITPQNLGFLAEAAARFGSERIITAIDGIKNPPESGLHRLEAIMPDTDNKHGLELVS
WVKQIEKQGTGAILLTSRDTDGTKSGYDLEMTKAVAQAVSIPVIASGGAGSMEDIYAVITGGKAQAVLAASVFHSGMINI
MELKKYLYSRGIPVNMPPLR
>Mature_260_residues
MNPIKIIPCLDVKNNRVTKAIEFTRMADSYDQAEAALAYSGQGADELAFLDMTAILEGSAIHTDWVKKIIGNTDLPLLAG
GGIRDLRDIKSLLSLGVSRVALNNITPQNLGFLAEAAARFGSERIITAIDGIKNPPESGLHRLEAIMPDTDNKHGLELVS
WVKQIEKQGTGAILLTSRDTDGTKSGYDLEMTKAVAQAVSIPVIASGGAGSMEDIYAVITGGKAQAVLAASVFHSGMINI
MELKKYLYSRGIPVNMPPLR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=254, Percent_Identity=35.0393700787402, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI87082028, Length=247, Percent_Identity=23.8866396761134, Blast_Score=65, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=26.1980830670927, Blast_Score=93, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 27804; Mature: 27804

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIKIIPCLDVKNNRVTKAIEFTRMADSYDQAEAALAYSGQGADELAFLDMTAILEGSA
CCCEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHEECCCCCCHHHHHHHHHHHCCCC
IHTDWVKKIIGNTDLPLLAGGGIRDLRDIKSLLSLGVSRVALNNITPQNLGFLAEAAARF
CCHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GSERIITAIDGIKNPPESGLHRLEAIMPDTDNKHGLELVSWVKQIEKQGTGAILLTSRDT
CCCCEEEHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCC
DGTKSGYDLEMTKAVAQAVSIPVIASGGAGSMEDIYAVITGGKAQAVLAASVFHSGMINI
CCCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHEEEEEECCCHHHHHHHHHHHHCCHHH
MELKKYLYSRGIPVNMPPLR
HHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNPIKIIPCLDVKNNRVTKAIEFTRMADSYDQAEAALAYSGQGADELAFLDMTAILEGSA
CCCEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHEECCCCCCHHHHHHHHHHHCCCC
IHTDWVKKIIGNTDLPLLAGGGIRDLRDIKSLLSLGVSRVALNNITPQNLGFLAEAAARF
CCHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GSERIITAIDGIKNPPESGLHRLEAIMPDTDNKHGLELVSWVKQIEKQGTGAILLTSRDT
CCCCEEEHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCC
DGTKSGYDLEMTKAVAQAVSIPVIASGGAGSMEDIYAVITGGKAQAVLAASVFHSGMINI
CCCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHEEEEEECCCHHHHHHHHHHHHCCHHH
MELKKYLYSRGIPVNMPPLR
HHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA