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The map label for this gene is bioD

Identifier: 38233782

GI number: 38233782

Start: 1191000

End: 1191668

Strand: Direct

Name: bioD

Synonym: DIP1192

Alternate gene names: 38233782

Gene position: 1191000-1191668 (Clockwise)

Preceding gene: 38233781

Following gene: 38233783

Centisome position: 47.86

GC content: 48.13

Gene sequence:

>669_bases
ATGGCAATCGTAGTTGTAACCGGTACAAACACAGATGTAGGAAAGACAATCGCTTCCGCTGCAGTATGTCAGCATTATTC
ACGACAAGGATTCCGAGTCGTTCCAGTTAAGCCCGTCCAAACTGGAGAACCAAAAGGTTCAGGTGATGCACAAACCATTG
AAAAACTAACCGGAATAGTAGGCAAATGCTTTGCCCGATTCCCAGAACCACTCGCGCCTAATCTTTCTGCACAGCGGGCT
GGTATGCAGCAGTTAAATTTGGAAGAAATAGTTAATAAAATTCGTGAACTCGATGGGCCTCAAACAGTTGTTGTTGTCGA
GGGGGCCGGAGGACTCTTAGTTCGTTTGGCGGATTCATTTACGATCGCAGATGTTGCGGCGCAGCTAGACGCACCTCTCA
TCGTAGTAACAAATATGGCGTTGGGCTCGCTCAATGCGGCAGAACTCACTGTAGAAGCAGCGCAGCGACGTGGCTTGAAG
GTTTTAGGGCTGATTGGTGGCAGTATGCCTAAGAATCCTGACTTGGCTACCTCGCTTAATGTTGCAGAAATGGAAAAGGT
GACTGGCATACCGTTGTGGGGTTCTATCGCAGAAGGTGCAGGGCAATTGAGCAAGGAAGCCTTTTGTCAACTAGTAGAAG
ATCTACATCTTCCCACTATGTGGCCGTAA

Upstream 100 bases:

>100_bases
ATTTGTCTGTACTCATAAACAAATACAAAAGATTTCGTGTGCAATTGCTTCTATCGTGCATACGGAGCAGGAACGTTTCG
AACGCTAAAGGAGAAATGTA

Downstream 100 bases:

>100_bases
TCCGAGCAGGCCGAGGCCCCCTGATCGACCGGGCGGTGAGCTCCTTAGAGATAGTGCAATCGCTATGATTGATTACGTCA
CTGTACTCAGTTAATTCAAG

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MAIVVVTGTNTDVGKTIASAAVCQHYSRQGFRVVPVKPVQTGEPKGSGDAQTIEKLTGIVGKCFARFPEPLAPNLSAQRA
GMQQLNLEEIVNKIRELDGPQTVVVVEGAGGLLVRLADSFTIADVAAQLDAPLIVVTNMALGSLNAAELTVEAAQRRGLK
VLGLIGGSMPKNPDLATSLNVAEMEKVTGIPLWGSIAEGAGQLSKEAFCQLVEDLHLPTMWP

Sequences:

>Translated_222_residues
MAIVVVTGTNTDVGKTIASAAVCQHYSRQGFRVVPVKPVQTGEPKGSGDAQTIEKLTGIVGKCFARFPEPLAPNLSAQRA
GMQQLNLEEIVNKIRELDGPQTVVVVEGAGGLLVRLADSFTIADVAAQLDAPLIVVTNMALGSLNAAELTVEAAQRRGLK
VLGLIGGSMPKNPDLATSLNVAEMEKVTGIPLWGSIAEGAGQLSKEAFCQLVEDLHLPTMWP
>Mature_221_residues
AIVVVTGTNTDVGKTIASAAVCQHYSRQGFRVVPVKPVQTGEPKGSGDAQTIEKLTGIVGKCFARFPEPLAPNLSAQRAG
MQQLNLEEIVNKIRELDGPQTVVVVEGAGGLLVRLADSFTIADVAAQLDAPLIVVTNMALGSLNAAELTVEAAQRRGLKV
LGLIGGSMPKNPDLATSLNVAEMEKVTGIPLWGSIAEGAGQLSKEAFCQLVEDLHLPTMWP

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=173, Percent_Identity=34.1040462427746, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081944, Length=189, Percent_Identity=31.7460317460317, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_CORDI (Q6NHE6)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939549.1
- HSSP:   P13000
- ProteinModelPortal:   Q6NHE6
- GeneID:   2649747
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1192
- NMPDR:   fig|257309.1.peg.1140
- HOGENOM:   HBG650065
- OMA:   IDTIVAC
- PhylomeDB:   Q6NHE6
- ProtClustDB:   PRK00090
- BioCyc:   CDIP257309:DIP1192-MONOMER
- BRENDA:   6.3.3.3
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 23291; Mature: 23160

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIVVVTGTNTDVGKTIASAAVCQHYSRQGFRVVPVKPVQTGEPKGSGDAQTIEKLTGIV
CEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHH
GKCFARFPEPLAPNLSAQRAGMQQLNLEEIVNKIRELDGPQTVVVVEGAGGLLVRLADSF
HHHHHHCCCCCCCCCCHHHCCHHCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCH
TIADVAAQLDAPLIVVTNMALGSLNAAELTVEAAQRRGLKVLGLIGGSMPKNPDLATSLN
HHHHHHHHCCCCEEEEECCHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHCCC
VAEMEKVTGIPLWGSIAEGAGQLSKEAFCQLVEDLHLPTMWP
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AIVVVTGTNTDVGKTIASAAVCQHYSRQGFRVVPVKPVQTGEPKGSGDAQTIEKLTGIV
EEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHH
GKCFARFPEPLAPNLSAQRAGMQQLNLEEIVNKIRELDGPQTVVVVEGAGGLLVRLADSF
HHHHHHCCCCCCCCCCHHHCCHHCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCH
TIADVAAQLDAPLIVVTNMALGSLNAAELTVEAAQRRGLKVLGLIGGSMPKNPDLATSLN
HHHHHHHHCCCCEEEEECCHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHCCC
VAEMEKVTGIPLWGSIAEGAGQLSKEAFCQLVEDLHLPTMWP
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910