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The map label for this gene is ddl

Identifier: 38233721

GI number: 38233721

Start: 1116334

End: 1117431

Strand: Direct

Name: ddl

Synonym: DIP1131

Alternate gene names: 38233721

Gene position: 1116334-1117431 (Clockwise)

Preceding gene: 38233720

Following gene: 38233723

Centisome position: 44.86

GC content: 52.28

Gene sequence:

>1098_bases
GTGAGCCAAAATCTTTCAGCACAGAACTCTTCAGAAACTCCTCGTATTAAAGTTGCCATCATCTATGGTGGTCGTAGCTC
AGAACACTCCGTGTCGTGTGTGTCTGCCGGCGCTATCATGGCGCACCTTGATCCGCAGCGCTACGAGGTGTTCCCAGTAG
GTATTACGCACGACGGTGTGTGGACTGTGGGGGAATCAGATCCAAGCCGGCTTAAGACTGTCGACCGCGTGATGCCCGAG
GTTCAGTTCACAAGGGAAGTATCGCTGTCGGTCAACCCAACTACCGCTGGCGAACTATGCTTCGAGGATGGATCGCTTTA
TGCCAAAGTCGATGTAGTTTTCCCAGTCTTGCATGGTCGCTTTGGCGAAGACGGTACGATCCAAGGCCTATTTGAGCTAT
CTGGTGTGCCTTACGTTGGAACTGGTGTGTTGAGCTCCGCCTGCGGAATGGACAAGGAATTTACTAAGAAACTCATGGCG
GCTGAAGGGCTGCCAGTTGGCAAGGAAGTTATCCTTCGCGGCTCAGAAACGTTGACTGAAGAACATAAGCGTGAGCTTGG
CTTGCCTGTGTTTGTTAAACCTGCTCGTGGTGGATCATCCATTGGTATTTCTCGTGTAGCTGATTGGAGCGAATGGGACG
CGGCATTGAGTCTTGCACGCGAGCACGATTCCAAGGTGATCGTCGAGGCTGAAATTGTGGGTGTAGAAGTCGAATGCGGC
GTGCTTGAGCGTATCGACGGTTCCCTGATGGCGTCTGTTCCCGCCCAGCTGCAAGATACCGATGAGGGTGACGAAGGTTT
CTATGGTTTTGATACCAAGTACCTAGACGATGTGGTCACCGCTCACATTCCAGCGCCCTTCGACGCGGAAACTACTGCGT
TGATTCAAGAGCTGTCGCTGAAGGCTTTCACAGCTTTAAGCTGCCGTGGTTTGGCACGCGTGGACTTCTTTGTCACCGAC
CATGGACCTGTGCTCAACGAGATCAATACCATGCCAGGATTTACCCCTATTTCGATGTACCCCCAGGTATTCGAGGCAAC
AGGTATCGGTTATGCGCAGCTGCTCGATAACTTGATCGAGCAGGCCTTGCACAAATAA

Upstream 100 bases:

>100_bases
TTTGGAGTATGTCATCGTGACCAAAATGTTGCCGACATGGTGGCTGCATTGATGGGGCGTACCAAGAAATCTGAGTAGCT
ACAGGGTAGAGTTTCCGTCT

Downstream 100 bases:

>100_bases
TTTGCGTTAGCGGATCGACTCCAACGCCTCAGTAATTGCTACGAGCGCGGCGTTACCAGCCTCATGCGGCAGGTTCACCG
CGACCGTAGCTTTTTGATCT

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSQNLSAQNSSETPRIKVAIIYGGRSSEHSVSCVSAGAIMAHLDPQRYEVFPVGITHDGVWTVGESDPSRLKTVDRVMPE
VQFTREVSLSVNPTTAGELCFEDGSLYAKVDVVFPVLHGRFGEDGTIQGLFELSGVPYVGTGVLSSACGMDKEFTKKLMA
AEGLPVGKEVILRGSETLTEEHKRELGLPVFVKPARGGSSIGISRVADWSEWDAALSLAREHDSKVIVEAEIVGVEVECG
VLERIDGSLMASVPAQLQDTDEGDEGFYGFDTKYLDDVVTAHIPAPFDAETTALIQELSLKAFTALSCRGLARVDFFVTD
HGPVLNEINTMPGFTPISMYPQVFEATGIGYAQLLDNLIEQALHK

Sequences:

>Translated_365_residues
MSQNLSAQNSSETPRIKVAIIYGGRSSEHSVSCVSAGAIMAHLDPQRYEVFPVGITHDGVWTVGESDPSRLKTVDRVMPE
VQFTREVSLSVNPTTAGELCFEDGSLYAKVDVVFPVLHGRFGEDGTIQGLFELSGVPYVGTGVLSSACGMDKEFTKKLMA
AEGLPVGKEVILRGSETLTEEHKRELGLPVFVKPARGGSSIGISRVADWSEWDAALSLAREHDSKVIVEAEIVGVEVECG
VLERIDGSLMASVPAQLQDTDEGDEGFYGFDTKYLDDVVTAHIPAPFDAETTALIQELSLKAFTALSCRGLARVDFFVTD
HGPVLNEINTMPGFTPISMYPQVFEATGIGYAQLLDNLIEQALHK
>Mature_364_residues
SQNLSAQNSSETPRIKVAIIYGGRSSEHSVSCVSAGAIMAHLDPQRYEVFPVGITHDGVWTVGESDPSRLKTVDRVMPEV
QFTREVSLSVNPTTAGELCFEDGSLYAKVDVVFPVLHGRFGEDGTIQGLFELSGVPYVGTGVLSSACGMDKEFTKKLMAA
EGLPVGKEVILRGSETLTEEHKRELGLPVFVKPARGGSSIGISRVADWSEWDAALSLAREHDSKVIVEAEIVGVEVECGV
LERIDGSLMASVPAQLQDTDEGDEGFYGFDTKYLDDVVTAHIPAPFDAETTALIQELSLKAFTALSCRGLARVDFFVTDH
GPVLNEINTMPGFTPISMYPQVFEATGIGYAQLLDNLIEQALHK

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=357, Percent_Identity=38.375350140056, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1786280, Length=353, Percent_Identity=34.5609065155807, Blast_Score=172, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_CORDI (Q6NHK6)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939488.1
- HSSP:   P25051
- ProteinModelPortal:   Q6NHK6
- GeneID:   2649933
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1131
- NMPDR:   fig|257309.1.peg.1079
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- PhylomeDB:   Q6NHK6
- ProtClustDB:   PRK01966
- BioCyc:   CDIP257309:DIP1131-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 39428; Mature: 39297

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNLSAQNSSETPRIKVAIIYGGRSSEHSVSCVSAGAIMAHLDPQRYEVFPVGITHDGV
CCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCEEEEECCCCCEEEEEEEECCCCE
WTVGESDPSRLKTVDRVMPEVQFTREVSLSVNPTTAGELCFEDGSLYAKVDVVFPVLHGR
EEECCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCEEEECCCEEEEEEEEEEHHCCC
FGEDGTIQGLFELSGVPYVGTGVLSSACGMDKEFTKKLMAAEGLPVGKEVILRGSETLTE
CCCCCCEEEEEECCCCCEECHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHEECCCHHHHH
EHKRELGLPVFVKPARGGSSIGISRVADWSEWDAALSLAREHDSKVIVEAEIVGVEVECG
HHHHHCCCCEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHCCCEEEEEEEEEEEEEECC
VLERIDGSLMASVPAQLQDTDEGDEGFYGFDTKYLDDVVTAHIPAPFDAETTALIQELSL
HHHHCCCCCEECCCCCCCCCCCCCCCEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
KAFTALSCRGLARVDFFVTDHGPVLNEINTMPGFTPISMYPQVFEATGIGYAQLLDNLIE
HHHHHHHCCCCEEEEEEEECCCCHHHHHCCCCCCCCHHHCHHHHHHCCCCHHHHHHHHHH
QALHK
HHHCC
>Mature Secondary Structure 
SQNLSAQNSSETPRIKVAIIYGGRSSEHSVSCVSAGAIMAHLDPQRYEVFPVGITHDGV
CCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCEEEEECCCCCEEEEEEEECCCCE
WTVGESDPSRLKTVDRVMPEVQFTREVSLSVNPTTAGELCFEDGSLYAKVDVVFPVLHGR
EEECCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCEEEECCCEEEEEEEEEEHHCCC
FGEDGTIQGLFELSGVPYVGTGVLSSACGMDKEFTKKLMAAEGLPVGKEVILRGSETLTE
CCCCCCEEEEEECCCCCEECHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHEECCCHHHHH
EHKRELGLPVFVKPARGGSSIGISRVADWSEWDAALSLAREHDSKVIVEAEIVGVEVECG
HHHHHCCCCEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHCCCEEEEEEEEEEEEEECC
VLERIDGSLMASVPAQLQDTDEGDEGFYGFDTKYLDDVVTAHIPAPFDAETTALIQELSL
HHHHCCCCCEECCCCCCCCCCCCCCCEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
KAFTALSCRGLARVDFFVTDHGPVLNEINTMPGFTPISMYPQVFEATGIGYAQLLDNLIE
HHHHHHHCCCCEEEEEEEECCCCHHHHHCCCCCCCCHHHCHHHHHHCCCCHHHHHHHHHH
QALHK
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910