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The map label for this gene is amyX [H]

Identifier: 38233498

GI number: 38233498

Start: 871597

End: 873411

Strand: Reverse

Name: amyX [H]

Synonym: DIP0902

Alternate gene names: 38233498

Gene position: 873411-871597 (Counterclockwise)

Preceding gene: 38233499

Following gene: 38233497

Centisome position: 35.1

GC content: 59.12

Gene sequence:

>1815_bases
ATGACCACAACCAACTCCTACACCGGCCAACTCGGCGCTTTCCTCACTCCCCACGGGGACACCTGTTTTCGAGTCTGGTC
TCCCGACGCCGCAACCGCCGACCTCCTCATCGAAAACCACACCGTGAGCATGCACCCCATCGGCGATGGGGTGTGGGAAG
CAAAACTTGATGGTGATCGACACGGTCAACGCTATAGTTACCGTCTTTCCCTCCGTGGGGAAACGATAGAATCAGTAGAC
CCCTATGCTCGGGCGGTCACGGCTAACGGAACGCATGGGGTCGTCGTCACGCAATCCACCCCTGTGCCCCGCATGCCAGC
CTTCACCGCGCCCACCGACGCCATTATTTACGAAGCACACGTACGCGACCTGAGCATCGCCCCCAACAGCGGAATCCACA
ACAAAGGCCGCTTCCTAGGACTCACCGAACGCGACACCCACACCACCGCTGGCAACCCCAGCGGCCTCGACTACATCCTA
AGCCTCGGGGTCACCCACATCCAGCTCCTGCCGATTTTCGACTTCGGCTCCGTCGACGAAACCGGCGACCTTAGCTACAA
CGCCCAATACAACTGGGGCTACGACCCCATGAATTACAACGCGCCCGAAGGCTCCTACTCCAGCAACCCCAACGACCCCT
TCACGCGCATCGACGAACTCAAACACACCATCCGCACGCTTCACGACGCAGGCCTGCGCGTCATCATGGACGTCGTCTAC
AACCACGTCTACGACACCACCACCTCCCCACTCGAACGCACCGCCCCCGGTCACTACTTCCGCATGACCGACGACGGCAC
CTTCCACGACGGCACCTTCTGCGGCAACGAAACCGCCTCCGAAAACCCCATGATGCGCAAATTCATCATCGACTCCGTCA
CCCACTGGGCCACCGAATACGGCCTCGACGGCTTCCGATTCGACCTCATGGGCATCCACGACGTCGACACCATGAACGCC
ATCCGCACAGCCCTCGACGCCATCGACCCCTCCATCATCATCCTCGGCGAAGGCTGGAACCTCGGCAACCACCCACCGCA
CATCCTCCCCGCAAACCAACACAACGCCGCCCACATGCCACGCATCGCCCACTTCAACGACTCCCTCCGCGACACCCTCA
AAGGAAGCACATTCCACTCCAACTCCCGCGGATTACTCACCGGCGACCACAACCCCAAACACATGTGGACCCTCTTCAAC
AACATCAAAGGCGCCCAACACCTCCCAGGACTCAACTTCACCAGCGCCAACCACACCGTCAACTACATCGAAGCCCACGA
CAACCACACCCTCTACGACCGCCTCCACCTCCTCCTACCCGACGCGCCTCACGACGAGCTCATCCGACGCTGCCAACTCG
CCACCACCATCCAATACCTCGCCTGCGGAATCACCTTCATCCACGCAGGACAAGAATTCGCACGCACAAAAAACAACACC
GAAAACTCCTACAACTCACCCGACCACATCAACGCCATCGACTACGACCGCGCCGCACAACACCCCCACAGCGTCCAACT
CCTTCGCGACCTCAACAACTTCAGAAAAACCCACACCGAGCTATTCCCCACCGAATACGACGACATCAACGAGCTCTACG
AAGCAACGCTCGTAAGCGGTGACTTCCTCACCTACTCCGTGGGGAAACAAACAACAGTCGCCATTAACTTTTCAACGCAA
CCACGCTCGATCCCCACGGAGGGATACTTCGCAGTGCTTCTCGACGACCATGTGTTCCCCACGGACAAACAGATTGAAAC
GAACTCGTGGACGGTCGCGCCTCTCTCCGTGAGCATCCTCCAGCAAGTTAGTTGA

Upstream 100 bases:

>100_bases
GCGCGCGAGCACCTCGCACGCGGGATTTTTCTCACTCCACCCACCCATCATGAGACAATAAACAACTGCACAGTTTCCCC
ACGGGAAGGAGAACCCCACC

Downstream 100 bases:

>100_bases
TAGAAAACCGCACGTTGTGGTATTGTTTCGTCCGTCTCGGCGAGGGCTGCGAATTTCTCTGAAACATCAAAGCCGTTATC
GACGCGATAGTATTCAAAAG

Product: putative hydrolase

Products: NA

Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]

Number of amino acids: Translated: 604; Mature: 603

Protein sequence:

>604_residues
MTTTNSYTGQLGAFLTPHGDTCFRVWSPDAATADLLIENHTVSMHPIGDGVWEAKLDGDRHGQRYSYRLSLRGETIESVD
PYARAVTANGTHGVVVTQSTPVPRMPAFTAPTDAIIYEAHVRDLSIAPNSGIHNKGRFLGLTERDTHTTAGNPSGLDYIL
SLGVTHIQLLPIFDFGSVDETGDLSYNAQYNWGYDPMNYNAPEGSYSSNPNDPFTRIDELKHTIRTLHDAGLRVIMDVVY
NHVYDTTTSPLERTAPGHYFRMTDDGTFHDGTFCGNETASENPMMRKFIIDSVTHWATEYGLDGFRFDLMGIHDVDTMNA
IRTALDAIDPSIIILGEGWNLGNHPPHILPANQHNAAHMPRIAHFNDSLRDTLKGSTFHSNSRGLLTGDHNPKHMWTLFN
NIKGAQHLPGLNFTSANHTVNYIEAHDNHTLYDRLHLLLPDAPHDELIRRCQLATTIQYLACGITFIHAGQEFARTKNNT
ENSYNSPDHINAIDYDRAAQHPHSVQLLRDLNNFRKTHTELFPTEYDDINELYEATLVSGDFLTYSVGKQTTVAINFSTQ
PRSIPTEGYFAVLLDDHVFPTDKQIETNSWTVAPLSVSILQQVS

Sequences:

>Translated_604_residues
MTTTNSYTGQLGAFLTPHGDTCFRVWSPDAATADLLIENHTVSMHPIGDGVWEAKLDGDRHGQRYSYRLSLRGETIESVD
PYARAVTANGTHGVVVTQSTPVPRMPAFTAPTDAIIYEAHVRDLSIAPNSGIHNKGRFLGLTERDTHTTAGNPSGLDYIL
SLGVTHIQLLPIFDFGSVDETGDLSYNAQYNWGYDPMNYNAPEGSYSSNPNDPFTRIDELKHTIRTLHDAGLRVIMDVVY
NHVYDTTTSPLERTAPGHYFRMTDDGTFHDGTFCGNETASENPMMRKFIIDSVTHWATEYGLDGFRFDLMGIHDVDTMNA
IRTALDAIDPSIIILGEGWNLGNHPPHILPANQHNAAHMPRIAHFNDSLRDTLKGSTFHSNSRGLLTGDHNPKHMWTLFN
NIKGAQHLPGLNFTSANHTVNYIEAHDNHTLYDRLHLLLPDAPHDELIRRCQLATTIQYLACGITFIHAGQEFARTKNNT
ENSYNSPDHINAIDYDRAAQHPHSVQLLRDLNNFRKTHTELFPTEYDDINELYEATLVSGDFLTYSVGKQTTVAINFSTQ
PRSIPTEGYFAVLLDDHVFPTDKQIETNSWTVAPLSVSILQQVS
>Mature_603_residues
TTTNSYTGQLGAFLTPHGDTCFRVWSPDAATADLLIENHTVSMHPIGDGVWEAKLDGDRHGQRYSYRLSLRGETIESVDP
YARAVTANGTHGVVVTQSTPVPRMPAFTAPTDAIIYEAHVRDLSIAPNSGIHNKGRFLGLTERDTHTTAGNPSGLDYILS
LGVTHIQLLPIFDFGSVDETGDLSYNAQYNWGYDPMNYNAPEGSYSSNPNDPFTRIDELKHTIRTLHDAGLRVIMDVVYN
HVYDTTTSPLERTAPGHYFRMTDDGTFHDGTFCGNETASENPMMRKFIIDSVTHWATEYGLDGFRFDLMGIHDVDTMNAI
RTALDAIDPSIIILGEGWNLGNHPPHILPANQHNAAHMPRIAHFNDSLRDTLKGSTFHSNSRGLLTGDHNPKHMWTLFNN
IKGAQHLPGLNFTSANHTVNYIEAHDNHTLYDRLHLLLPDAPHDELIRRCQLATTIQYLACGITFIHAGQEFARTKNNTE
NSYNSPDHINAIDYDRAAQHPHSVQLLRDLNNFRKTHTELFPTEYDDINELYEATLVSGDFLTYSVGKQTTVAINFSTQP
RSIPTEGYFAVLLDDHVFPTDKQIETNSWTVAPLSVSILQQVS

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=380, Percent_Identity=25.7894736842105, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR011840 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.41 [H]

Molecular weight: Translated: 67479; Mature: 67347

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTNSYTGQLGAFLTPHGDTCFRVWSPDAATADLLIENHTVSMHPIGDGVWEAKLDGDR
CCCCCCCCCCCCEEECCCCCCEEEEECCCCCCEEEEEECCEEEEEECCCCEEEEECCCCC
HGQRYSYRLSLRGETIESVDPYARAVTANGTHGVVVTQSTPVPRMPAFTAPTDAIIYEAH
CCCEEEEEEEECCCCHHHCCCHHEEEECCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEE
VRDLSIAPNSGIHNKGRFLGLTERDTHTTAGNPSGLDYILSLGVTHIQLLPIFDFGSVDE
CCEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHCCCCEEEEEEEECCCCCCC
TGDLSYNAQYNWGYDPMNYNAPEGSYSSNPNDPFTRIDELKHTIRTLHDAGLRVIMDVVY
CCCEEECEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NHVYDTTTSPLERTAPGHYFRMTDDGTFHDGTFCGNETASENPMMRKFIIDSVTHWATEY
HHHHCCCCCCHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GLDGFRFDLMGIHDVDTMNAIRTALDAIDPSIIILGEGWNLGNHPPHILPANQHNAAHMP
CCCCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCCCCCCCC
RIAHFNDSLRDTLKGSTFHSNSRGLLTGDHNPKHMWTLFNNIKGAQHLPGLNFTSANHTV
EEHHCCHHHHHHHCCCCEECCCCCEEECCCCCCCHHHHHHCCCCCHHCCCCCCCCCCCEE
NYIEAHDNHTLYDRLHLLLPDAPHDELIRRCQLATTIQYLACGITFIHAGQEFARTKNNT
EEEEECCCCCHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHCCCCC
ENSYNSPDHINAIDYDRAAQHPHSVQLLRDLNNFRKTHTELFPTEYDDINELYEATLVSG
CCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
DFLTYSVGKQTTVAINFSTQPRSIPTEGYFAVLLDDHVFPTDKQIETNSWTVAPLSVSIL
CEEEEECCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCEEECCCEEEEEHHHHHH
QQVS
HHCC
>Mature Secondary Structure 
TTTNSYTGQLGAFLTPHGDTCFRVWSPDAATADLLIENHTVSMHPIGDGVWEAKLDGDR
CCCCCCCCCCCEEECCCCCCEEEEECCCCCCEEEEEECCEEEEEECCCCEEEEECCCCC
HGQRYSYRLSLRGETIESVDPYARAVTANGTHGVVVTQSTPVPRMPAFTAPTDAIIYEAH
CCCEEEEEEEECCCCHHHCCCHHEEEECCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEE
VRDLSIAPNSGIHNKGRFLGLTERDTHTTAGNPSGLDYILSLGVTHIQLLPIFDFGSVDE
CCEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHCCCCEEEEEEEECCCCCCC
TGDLSYNAQYNWGYDPMNYNAPEGSYSSNPNDPFTRIDELKHTIRTLHDAGLRVIMDVVY
CCCEEECEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NHVYDTTTSPLERTAPGHYFRMTDDGTFHDGTFCGNETASENPMMRKFIIDSVTHWATEY
HHHHCCCCCCHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GLDGFRFDLMGIHDVDTMNAIRTALDAIDPSIIILGEGWNLGNHPPHILPANQHNAAHMP
CCCCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCCCCCCCC
RIAHFNDSLRDTLKGSTFHSNSRGLLTGDHNPKHMWTLFNNIKGAQHLPGLNFTSANHTV
EEHHCCHHHHHHHCCCCEECCCCCEEECCCCCCCHHHHHHCCCCCHHCCCCCCCCCCCEE
NYIEAHDNHTLYDRLHLLLPDAPHDELIRRCQLATTIQYLACGITFIHAGQEFARTKNNT
EEEEECCCCCHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHCCCCC
ENSYNSPDHINAIDYDRAAQHPHSVQLLRDLNNFRKTHTELFPTEYDDINELYEATLVSG
CCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
DFLTYSVGKQTTVAINFSTQPRSIPTEGYFAVLLDDHVFPTDKQIETNSWTVAPLSVSIL
CEEEEECCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCEEECCCEEEEEHHHHHH
QQVS
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]