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The map label for this gene is prsA [H]

Identifier: 38233499

GI number: 38233499

Start: 873535

End: 874515

Strand: Reverse

Name: prsA [H]

Synonym: DIP0903

Alternate gene names: 38233499

Gene position: 874515-873535 (Counterclockwise)

Preceding gene: 38233500

Following gene: 38233498

Centisome position: 35.14

GC content: 60.75

Gene sequence:

>981_bases
ATGACTTCGCACAACTGGACCGCGAACCAGAAGAATTTGATGGTATTAACTGGTCGCGCCCACCCAGAGCTTGCCGACGC
CGTCGCAAAGGAACTGGGCGTAGAAATCACCCCCACCACCGCACGCGACTTTGCCAACGGTGAAATCTTCGTCCGCTTCG
AAGAGTCCGTCCGTGGCTGCGACGCATTCGTCCTCCAGTCACACACCCAGCCACTCAACAAGTGGCTGATGGAGCAGCTC
ATCATGATCGACGCCCTCAAGCGTGGATCCGCCAAGCGCATCACCGCAATCCTGCCGTTCTACCCTTACGCACGTCAGGA
CAAGAAGCACCGCGGCCGCGAGCCCATCTCCGCTCGCCTCGTCGCAGATTTGCTCCGCACCGCCGGCGCTGACCGCATCG
TCTCCGTCGACCTGCACACTGACCAGATCCAAGGCTTCTTCGACGGTCCAGTCGACCACATGCACGCGATGCCAATCCTC
ACGGACTATATCAAGGGCAAGTACGATCTCGACAACGTCGTTGTCGTCTCCCCAGACGCCGGCCGCGTTAAGGTCGCTGA
AAAGTGGGCCAACACACTGGGCGACGCCCCCATGGCCTTCGTCCACAAGACCCGCTCCGTTGACGTTGCCAACCAAGTCG
TCGCCAACCGTGTTGTCGGCGATGTAGCTGGCAAGACCGCCATCCTTCTCGACGACATGATCGACACCGGCGGAACCATC
GCCGGCGCAGTCGGAGTCCTTCGCGACGCCGGTGCCGAAGACGTCATCATCGCCTGCACCCACGGCGTATTCTCCGGCCC
AGCCCGCGAACGCCTCTCCCAGTGCGGCGCCAAGGAAGTCATCACCACCGACACTCTGCCACAGTCCACCGAAGGCTGGG
ACAACCTCACCGTCTTGTCCATCGCACCACTGCTGGCAAAGACCATCCACGAAATTTTCGAAAACGGCTCTGTGACCACC
CTCTTCGAGGGCCAAGCTTAA

Upstream 100 bases:

>100_bases
ACATTGAAGGCTGGGTGGAAAAAAACCGCCCCGGCACTCCAGCTGCCGATGCCGCCCGTCAGGCACATGCCCACGAAACC
AAGGAAGGTTAAGCGAAAAC

Downstream 100 bases:

>100_bases
CCTCTCACGGAACACACAATCCCGCGCGCGAGCACCTCGCACGCGGGATTTTTCTCACTCCACCCACCCATCATGAGACA
ATAAACAACTGCACAGTTTC

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTSHNWTANQKNLMVLTGRAHPELADAVAKELGVEITPTTARDFANGEIFVRFEESVRGCDAFVLQSHTQPLNKWLMEQL
IMIDALKRGSAKRITAILPFYPYARQDKKHRGREPISARLVADLLRTAGADRIVSVDLHTDQIQGFFDGPVDHMHAMPIL
TDYIKGKYDLDNVVVVSPDAGRVKVAEKWANTLGDAPMAFVHKTRSVDVANQVVANRVVGDVAGKTAILLDDMIDTGGTI
AGAVGVLRDAGAEDVIIACTHGVFSGPARERLSQCGAKEVITTDTLPQSTEGWDNLTVLSIAPLLAKTIHEIFENGSVTT
LFEGQA

Sequences:

>Translated_326_residues
MTSHNWTANQKNLMVLTGRAHPELADAVAKELGVEITPTTARDFANGEIFVRFEESVRGCDAFVLQSHTQPLNKWLMEQL
IMIDALKRGSAKRITAILPFYPYARQDKKHRGREPISARLVADLLRTAGADRIVSVDLHTDQIQGFFDGPVDHMHAMPIL
TDYIKGKYDLDNVVVVSPDAGRVKVAEKWANTLGDAPMAFVHKTRSVDVANQVVANRVVGDVAGKTAILLDDMIDTGGTI
AGAVGVLRDAGAEDVIIACTHGVFSGPARERLSQCGAKEVITTDTLPQSTEGWDNLTVLSIAPLLAKTIHEIFENGSVTT
LFEGQA
>Mature_325_residues
TSHNWTANQKNLMVLTGRAHPELADAVAKELGVEITPTTARDFANGEIFVRFEESVRGCDAFVLQSHTQPLNKWLMEQLI
MIDALKRGSAKRITAILPFYPYARQDKKHRGREPISARLVADLLRTAGADRIVSVDLHTDQIQGFFDGPVDHMHAMPILT
DYIKGKYDLDNVVVVSPDAGRVKVAEKWANTLGDAPMAFVHKTRSVDVANQVVANRVVGDVAGKTAILLDDMIDTGGTIA
GAVGVLRDAGAEDVIIACTHGVFSGPARERLSQCGAKEVITTDTLPQSTEGWDNLTVLSIAPLLAKTIHEIFENGSVTTL
FEGQA

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506129, Length=314, Percent_Identity=42.9936305732484, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI4506127, Length=314, Percent_Identity=42.9936305732484, Blast_Score=245, Evalue=3e-65,
Organism=Homo sapiens, GI28557709, Length=314, Percent_Identity=42.6751592356688, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI84875539, Length=317, Percent_Identity=42.5867507886435, Blast_Score=241, Evalue=5e-64,
Organism=Homo sapiens, GI4506133, Length=331, Percent_Identity=31.7220543806647, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI194018537, Length=332, Percent_Identity=33.433734939759, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI310128524, Length=145, Percent_Identity=33.7931034482759, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI310115209, Length=145, Percent_Identity=33.7931034482759, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI310118259, Length=145, Percent_Identity=33.7931034482759, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI310119946, Length=145, Percent_Identity=33.7931034482759, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1787458, Length=315, Percent_Identity=42.5396825396825, Blast_Score=248, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI25149168, Length=314, Percent_Identity=42.9936305732484, Blast_Score=250, Evalue=7e-67,
Organism=Caenorhabditis elegans, GI17554702, Length=314, Percent_Identity=42.9936305732484, Blast_Score=249, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI71989924, Length=314, Percent_Identity=42.9936305732484, Blast_Score=248, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI17554704, Length=311, Percent_Identity=43.0868167202572, Blast_Score=247, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI17570245, Length=338, Percent_Identity=33.1360946745562, Blast_Score=178, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6320946, Length=314, Percent_Identity=41.7197452229299, Blast_Score=252, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6319403, Length=314, Percent_Identity=41.4012738853503, Blast_Score=246, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6321776, Length=316, Percent_Identity=40.8227848101266, Blast_Score=230, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6322667, Length=205, Percent_Identity=40.9756097560976, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6324511, Length=110, Percent_Identity=37.2727272727273, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21355239, Length=316, Percent_Identity=42.7215189873418, Blast_Score=248, Evalue=3e-66,
Organism=Drosophila melanogaster, GI45551540, Length=339, Percent_Identity=40.117994100295, Blast_Score=237, Evalue=7e-63,
Organism=Drosophila melanogaster, GI24651458, Length=357, Percent_Identity=30.5322128851541, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24651456, Length=357, Percent_Identity=30.5322128851541, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI281362873, Length=357, Percent_Identity=30.5322128851541, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24651454, Length=357, Percent_Identity=30.5322128851541, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24651462, Length=183, Percent_Identity=37.1584699453552, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24651464, Length=183, Percent_Identity=37.1584699453552, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI45552010, Length=183, Percent_Identity=37.1584699453552, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 35406; Mature: 35275

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00144 ASN_GLN_ASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSHNWTANQKNLMVLTGRAHPELADAVAKELGVEITPTTARDFANGEIFVRFEESVRGC
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCEECCCCHHHCCCCEEEEEEHHHCCCH
DAFVLQSHTQPLNKWLMEQLIMIDALKRGSAKRITAILPFYPYARQDKKHRGREPISARL
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCCCHHHHH
VADLLRTAGADRIVSVDLHTDQIQGFFDGPVDHMHAMPILTDYIKGKYDLDNVVVVSPDA
HHHHHHHCCCCCEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCC
GRVKVAEKWANTLGDAPMAFVHKTRSVDVANQVVANRVVGDVAGKTAILLDDMIDTGGTI
CCCHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCHH
AGAVGVLRDAGAEDVIIACTHGVFSGPARERLSQCGAKEVITTDTLPQSTEGWDNLTVLS
HHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCEEHHH
IAPLLAKTIHEIFENGSVTTLFEGQA
HHHHHHHHHHHHHCCCCEEEEECCCC
>Mature Secondary Structure 
TSHNWTANQKNLMVLTGRAHPELADAVAKELGVEITPTTARDFANGEIFVRFEESVRGC
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCEECCCCHHHCCCCEEEEEEHHHCCCH
DAFVLQSHTQPLNKWLMEQLIMIDALKRGSAKRITAILPFYPYARQDKKHRGREPISARL
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCCCCHHHHH
VADLLRTAGADRIVSVDLHTDQIQGFFDGPVDHMHAMPILTDYIKGKYDLDNVVVVSPDA
HHHHHHHCCCCCEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCC
GRVKVAEKWANTLGDAPMAFVHKTRSVDVANQVVANRVVGDVAGKTAILLDDMIDTGGTI
CCCHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCHH
AGAVGVLRDAGAEDVIIACTHGVFSGPARERLSQCGAKEVITTDTLPQSTEGWDNLTVLS
HHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCEEHHH
IAPLLAKTIHEIFENGSVTTLFEGQA
HHHHHHHHHHHHHCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]