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The map label for this gene is prfC [H]

Identifier: 38233491

GI number: 38233491

Start: 865500

End: 867134

Strand: Reverse

Name: prfC [H]

Synonym: DIP0895

Alternate gene names: 38233491

Gene position: 867134-865500 (Counterclockwise)

Preceding gene: 38233492

Following gene: 38233488

Centisome position: 34.84

GC content: 54.92

Gene sequence:

>1635_bases
ATGAGTTCTGTTGCGCAAGAAGCCCATCGCCGTCGTACATTTGCTGTTATTGCTCACCCTGATGCTGGTAAGTCAACGCT
GACGGAGGCGTTGGCGTTGCATGCGCATGTGATCAGCGAAGCTGGCGCGGTGCACGGCAAGGGTAATCGTAAGGCGACTG
TCTCTGACTGGATGGAGATGGAAAAGGATCGTGGTATTTCGATCGCTTCGTCTGCGCTGCAGTTTGAGTATGCCCCCGAG
GGCCATTCTGGCGAACCGTTCATGATCAACTTGGTGGATACCCCCGGCCACGCTGATTTTTCTGAGGATACGTACCGTGT
GCTGACTGCAGTTGATGCGGCAGTCATGCTTATCGACGCAGCCAAAGGCCTCGAGCCGCAAACTCTCAAACTTTTCCGTG
TATGTAAGGCCCGCGGTTTGCCGATCATTACTGTGATCAACAAGTGGGACCGCCCTGGCCGTACTCCGCTCGAGCTTGTC
GACGAAATCGTCACCGAAATCGATCTCCAACCTACTCCCCTGTTTTGGCCGGTTGGCGACGCCGGTGATTTCCGCGGTCT
TGCACGCATTAACCCCGATGGTGAAGCAGAAGAATACATTCGATTCCTGCGCACCGCTGGTGGATCCACCATTGCCCCAG
AGGAGCACTACACGCCGGAGGAAGCCTCCTCCCGTGAGGAAACGGCATGGGACACTGCTGCTGAAGAAGTCGAACTGCTG
GCGGCGGATGGAGCTGTGCATAATCAAGAGCTCTTCTTGGAGTGCACCACCTCACCGCTGATCTTTGCCTCTGCCATGTT
GAACTTCGGTGTGCATCAGATTTTGGATACTCTCTGCGAGCTCGCACCGCAGCCGCATGGCCGAGAATCAGATGAGCGTG
CTGTAGAGGCCGCAACTGGTGCTTTTGATTCCTCACGGGAAGTTACTGACGAGTTCTCTGGCGTTGTGTTTAAAGTACAA
GCTGGTATGGACAAAAATCACCGCGACTCCTTGGCATTTATGCGTGTGGTTTCTGGCGAGTTCGATCGTGGCATGCAGGT
AACGCATGCACAATCTGGGCGTAGTTTTTCCACGAAGTACGCCCTGACTGTGTTCGGCCGTACCCGTTCGACTGTGGAAT
CGGCGTTTCCTGGCGATATCGTGGGTCTTGTTAACGCGGGATCGTTGGCTCCTGGCGACACTATCTTTGCTGGTCGTAAG
GTTCAGTATCCTCCGATGCCACAGTTCGCTCCAGAGCACTTCCGCACACTGCGCGCAAAGTCGCTAGGAAAATACAAACA
GTTCCGTAAGGCATTGGATCAGTTGGCAGCTGAAGGCGTTGTTCAGATCCTGAAAAATGATGCGCGTGGCGACGCCGCCC
CCGTCATGGCTGCTGTTGGCCCCATGCAGTTCGAGGTCATGATGGCTCGTATGCAAAACGAGTACAACGTAGAAACCGTT
GCCGATCCCATCCCATACTCTGTTGCTCGTCGCACCACCCCAGAAACAGCTACAGAGCTGGCAAAGCAACGCGGCGTGGA
GATCTTCACGCGTACCGACGGCGAACTGGTTGCACTGTTTGGCGACAAGTGGAAACTCGCCTTCATCGAAAAAGAGCACC
CCGAATTCGAGCTGCTCCCCATGGTTGCTGATTAA

Upstream 100 bases:

>100_bases
GCTGGCAGCGTCCGATTGCGCGAAATCCCAAGGTTGCTTCTCGTGTTTTCGATGATCTGAGTTCGCAGCTTGGGGTGAGC
TGGGGCTAAGATATTTCCTC

Downstream 100 bases:

>100_bases
CAGCCAGTAAGCTTCACCACCGCCGCGCGAGCTTGAGTTCGCGCGGCAAATTCATATCATCACGCGCCCATGCCCCCGAT
TTTTCTAAGCGCTGATACAC

Product: putative peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MSSVAQEAHRRRTFAVIAHPDAGKSTLTEALALHAHVISEAGAVHGKGNRKATVSDWMEMEKDRGISIASSALQFEYAPE
GHSGEPFMINLVDTPGHADFSEDTYRVLTAVDAAVMLIDAAKGLEPQTLKLFRVCKARGLPIITVINKWDRPGRTPLELV
DEIVTEIDLQPTPLFWPVGDAGDFRGLARINPDGEAEEYIRFLRTAGGSTIAPEEHYTPEEASSREETAWDTAAEEVELL
AADGAVHNQELFLECTTSPLIFASAMLNFGVHQILDTLCELAPQPHGRESDERAVEAATGAFDSSREVTDEFSGVVFKVQ
AGMDKNHRDSLAFMRVVSGEFDRGMQVTHAQSGRSFSTKYALTVFGRTRSTVESAFPGDIVGLVNAGSLAPGDTIFAGRK
VQYPPMPQFAPEHFRTLRAKSLGKYKQFRKALDQLAAEGVVQILKNDARGDAAPVMAAVGPMQFEVMMARMQNEYNVETV
ADPIPYSVARRTTPETATELAKQRGVEIFTRTDGELVALFGDKWKLAFIEKEHPEFELLPMVAD

Sequences:

>Translated_544_residues
MSSVAQEAHRRRTFAVIAHPDAGKSTLTEALALHAHVISEAGAVHGKGNRKATVSDWMEMEKDRGISIASSALQFEYAPE
GHSGEPFMINLVDTPGHADFSEDTYRVLTAVDAAVMLIDAAKGLEPQTLKLFRVCKARGLPIITVINKWDRPGRTPLELV
DEIVTEIDLQPTPLFWPVGDAGDFRGLARINPDGEAEEYIRFLRTAGGSTIAPEEHYTPEEASSREETAWDTAAEEVELL
AADGAVHNQELFLECTTSPLIFASAMLNFGVHQILDTLCELAPQPHGRESDERAVEAATGAFDSSREVTDEFSGVVFKVQ
AGMDKNHRDSLAFMRVVSGEFDRGMQVTHAQSGRSFSTKYALTVFGRTRSTVESAFPGDIVGLVNAGSLAPGDTIFAGRK
VQYPPMPQFAPEHFRTLRAKSLGKYKQFRKALDQLAAEGVVQILKNDARGDAAPVMAAVGPMQFEVMMARMQNEYNVETV
ADPIPYSVARRTTPETATELAKQRGVEIFTRTDGELVALFGDKWKLAFIEKEHPEFELLPMVAD
>Mature_543_residues
SSVAQEAHRRRTFAVIAHPDAGKSTLTEALALHAHVISEAGAVHGKGNRKATVSDWMEMEKDRGISIASSALQFEYAPEG
HSGEPFMINLVDTPGHADFSEDTYRVLTAVDAAVMLIDAAKGLEPQTLKLFRVCKARGLPIITVINKWDRPGRTPLELVD
EIVTEIDLQPTPLFWPVGDAGDFRGLARINPDGEAEEYIRFLRTAGGSTIAPEEHYTPEEASSREETAWDTAAEEVELLA
ADGAVHNQELFLECTTSPLIFASAMLNFGVHQILDTLCELAPQPHGRESDERAVEAATGAFDSSREVTDEFSGVVFKVQA
GMDKNHRDSLAFMRVVSGEFDRGMQVTHAQSGRSFSTKYALTVFGRTRSTVESAFPGDIVGLVNAGSLAPGDTIFAGRKV
QYPPMPQFAPEHFRTLRAKSLGKYKQFRKALDQLAAEGVVQILKNDARGDAAPVMAAVGPMQFEVMMARMQNEYNVETVA
DPIPYSVARRTTPETATELAKQRGVEIFTRTDGELVALFGDKWKLAFIEKEHPEFELLPMVAD

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=527, Percent_Identity=24.6679316888046, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI19923640, Length=574, Percent_Identity=25.4355400696864, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI25306283, Length=405, Percent_Identity=26.6666666666667, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI25306287, Length=298, Percent_Identity=28.8590604026846, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI157426893, Length=158, Percent_Identity=32.9113924050633, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI94966754, Length=221, Percent_Identity=29.4117647058824, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=34.8993288590604, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI310132016, Length=116, Percent_Identity=37.0689655172414, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI310110807, Length=116, Percent_Identity=37.0689655172414, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI310123363, Length=116, Percent_Identity=37.0689655172414, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1790835, Length=533, Percent_Identity=41.4634146341463, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1789738, Length=534, Percent_Identity=27.7153558052434, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI48994988, Length=190, Percent_Identity=33.6842105263158, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788922, Length=152, Percent_Identity=35.5263157894737, Blast_Score=102, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17533571, Length=493, Percent_Identity=24.5436105476673, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17556745, Length=496, Percent_Identity=24.3951612903226, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17557151, Length=142, Percent_Identity=35.9154929577465, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71988819, Length=144, Percent_Identity=34.0277777777778, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71988811, Length=144, Percent_Identity=34.0277777777778, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17506493, Length=163, Percent_Identity=32.5153374233129, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=487, Percent_Identity=24.435318275154, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6322359, Length=532, Percent_Identity=24.2481203007519, Blast_Score=121, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323320, Length=158, Percent_Identity=34.1772151898734, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324707, Length=160, Percent_Identity=34.375, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320593, Length=160, Percent_Identity=34.375, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324166, Length=187, Percent_Identity=31.5508021390374, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24582462, Length=482, Percent_Identity=25.7261410788382, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI221458488, Length=531, Percent_Identity=23.5404896421846, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI78706572, Length=161, Percent_Identity=36.0248447204969, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28574573, Length=144, Percent_Identity=35.4166666666667, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU [H]

EC number: NA

Molecular weight: Translated: 59637; Mature: 59506

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVAQEAHRRRTFAVIAHPDAGKSTLTEALALHAHVISEAGAVHGKGNRKATVSDWMEM
CCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
EKDRGISIASSALQFEYAPEGHSGEPFMINLVDTPGHADFSEDTYRVLTAVDAAVMLIDA
HHCCCCEEEHHHEEEEECCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
AKGLEPQTLKLFRVCKARGLPIITVINKWDRPGRTPLELVDEIVTEIDLQPTPLFWPVGD
HCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCC
AGDFRGLARINPDGEAEEYIRFLRTAGGSTIAPEEHYTPEEASSREETAWDTAAEEVELL
CCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEE
AADGAVHNQELFLECTTSPLIFASAMLNFGVHQILDTLCELAPQPHGRESDERAVEAATG
ECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
AFDSSREVTDEFSGVVFKVQAGMDKNHRDSLAFMRVVSGEFDRGMQVTHAQSGRSFSTKY
CCCCCHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEE
ALTVFGRTRSTVESAFPGDIVGLVNAGSLAPGDTIFAGRKVQYPPMPQFAPEHFRTLRAK
EEEEECCCHHHHHHCCCCCEEEEEECCCCCCCCCEECCCEECCCCCCCCCHHHHHHHHHH
SLGKYKQFRKALDQLAAEGVVQILKNDARGDAAPVMAAVGPMQFEVMMARMQNEYNVETV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCHHH
ADPIPYSVARRTTPETATELAKQRGVEIFTRTDGELVALFGDKWKLAFIEKEHPEFELLP
CCCCCHHHHHCCCCHHHHHHHHHCCCEEEEECCCCEEEEECCCEEEEEEECCCCCEEEEC
MVAD
EECC
>Mature Secondary Structure 
SSVAQEAHRRRTFAVIAHPDAGKSTLTEALALHAHVISEAGAVHGKGNRKATVSDWMEM
CHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
EKDRGISIASSALQFEYAPEGHSGEPFMINLVDTPGHADFSEDTYRVLTAVDAAVMLIDA
HHCCCCEEEHHHEEEEECCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
AKGLEPQTLKLFRVCKARGLPIITVINKWDRPGRTPLELVDEIVTEIDLQPTPLFWPVGD
HCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCC
AGDFRGLARINPDGEAEEYIRFLRTAGGSTIAPEEHYTPEEASSREETAWDTAAEEVELL
CCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEE
AADGAVHNQELFLECTTSPLIFASAMLNFGVHQILDTLCELAPQPHGRESDERAVEAATG
ECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
AFDSSREVTDEFSGVVFKVQAGMDKNHRDSLAFMRVVSGEFDRGMQVTHAQSGRSFSTKY
CCCCCHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEE
ALTVFGRTRSTVESAFPGDIVGLVNAGSLAPGDTIFAGRKVQYPPMPQFAPEHFRTLRAK
EEEEECCCHHHHHHCCCCCEEEEEECCCCCCCCCEECCCEECCCCCCCCCHHHHHHHHHH
SLGKYKQFRKALDQLAAEGVVQILKNDARGDAAPVMAAVGPMQFEVMMARMQNEYNVETV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCHHH
ADPIPYSVARRTTPETATELAKQRGVEIFTRTDGELVALFGDKWKLAFIEKEHPEFELLP
CCCCCHHHHHCCCCHHHHHHHHHCCCEEEEECCCCEEEEECCCEEEEEEECCCCCEEEEC
MVAD
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA