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The map label for this gene is lpdA [H]

Identifier: 38233252

GI number: 38233252

Start: 615701

End: 617173

Strand: Direct

Name: lpdA [H]

Synonym: DIP0645

Alternate gene names: 38233252

Gene position: 615701-617173 (Clockwise)

Preceding gene: 38233251

Following gene: 38233253

Centisome position: 24.74

GC content: 54.72

Gene sequence:

>1473_bases
GTGGGATGTCTCCGATACACTTGGGCGTTGGACTCATCTAACCGGCACACCCATACTGGAGGAACAGTGACTCGAATCGT
GATTATCGGCGGCGGCCCCGCAGGCTATGAAGCAGCGTTGGCCGGCGCAAAATATGGCGCCGAAATCACCATTATTGAGG
ACCGTGGTCTAGGCGGCGCAGCAGTTATCAACGACTGCGTGCCATCCAAGTCGTTCATCGCCGGCGCAAACATTAAGACT
GACTTGCGCCGCGCCGACGACATGGGACTGAACAAGGGGATCGGCGAAGCACACCTGCTTATCGACGCCCTCAACAACCG
CGTTCAGGCCCTTGCTTACGAACAATCCTCCGATATTCGGGCGTCGATGGATGCTCACGGTGTGCGCGTTATCGACGGCC
GCGGCTCCTTTGATGATTACAACCCCAAGCAAACCGTGCACTACATCAAGGTCGACCGTGCTGATGGCACCACAGAAACT
ATTGAGTGCGACCTCGTTTTGATTGCTACTGGTGCAACCCCACGTATCCTTCCTGACGCGCAGCCAGACGGTGAACGAAT
CCTCACATGGCGTCAGATCTACGGGCTCACCGAATTGCCAGAGCACCTCATTGTGGTTGGCTCGGGTGTGACTGGTGCTG
AGTTTGTTTCTGCGTTCGCAGAGCTTGGTGTGAAAGTGACAATGGTCGCTTCGCGTGACCGCATTTTGCCTCACGACGAC
GCCGATGCTGCCGACGTCCTAGAAACCGTGCTAGCAGAACGTGGCGTGGCCTTGGAAAAGCATGCTCGCGTTGATTCTGT
TATCCGTACCGAGGACGGCGGCGTATGTGTGAAGACTTCCGATGGTCGAGAGATCTTCGGTTCTCACGCCTTGATGACCG
TCGGTTCTGTGCCCAACACCAAAGATCTTGGTCTAGAAAAAATTGGCATTGCGACAACACGTTCAGGCCACATTTGTGTA
GACCGCGTTTCTCGTACCAATGTCGCAGGCGTGTATGCAGGTGGCGACTGTACTGATCTATTCCCGCTGGCGTCAGTAGC
AGCTATGCAGGGGCGAATTGCCATGTACCACGCTCTTGGTGAGGGCGTAAAGCCGATTCGCATGAAGACTGTGGCTACCG
CAGTATTTACCCGTCCAGAAATTGCCGCTGTGGGTGTTACCCAGAAACAAATTGAGTCTGGTGAGGTAATTGCACGTACG
GTGATGTTGCCGCTGCAGACCAACCCGCGTGCCAAGATGCGTTCATTGCGCCACGGTTTTGTCAAGATGTTCTGCCGTAA
GAACTCCGGCATCGTGATCGGTGGCGTGGTTGTTGCTCCTACTGCCTCAGAACTGATTCTGCCGATTGCTGTTGCTGTGA
CAAACCAACTCACCGTGTCTGATCTGGCGGAGAGCTTTGCCGTCTATCCATCATTATCGGGTTCGATTACTGAGGCTGCG
CGTCAATTAGTGCAGCATGATGACCTGCAATAA

Upstream 100 bases:

>100_bases
CTTCGGTGCGGTAGCCGATCGCTACAACTCCCACAACGCGATGTTTATTGAGGATTAATCGCACGCGATTCCAGTGGGGG
TAAAGTGCGCGATTCGCGCT

Downstream 100 bases:

>100_bases
AACTGTAGGGGTAATCAAATAGGGGGTGTAGCCGAGGCGTTTTCTGTTTGCTACAGGACGCCTCGGTGTCATTTTATGAC
TTTAAATGTCTTGACCTCAT

Product: flavoprotein disulfide reductase

Products: NA

Alternate protein names: NAD(P)H quinone reductase; NAD(P)H: menadione oxidoreductase; NADH-menadione reductase [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MGCLRYTWALDSSNRHTHTGGTVTRIVIIGGGPAGYEAALAGAKYGAEITIIEDRGLGGAAVINDCVPSKSFIAGANIKT
DLRRADDMGLNKGIGEAHLLIDALNNRVQALAYEQSSDIRASMDAHGVRVIDGRGSFDDYNPKQTVHYIKVDRADGTTET
IECDLVLIATGATPRILPDAQPDGERILTWRQIYGLTELPEHLIVVGSGVTGAEFVSAFAELGVKVTMVASRDRILPHDD
ADAADVLETVLAERGVALEKHARVDSVIRTEDGGVCVKTSDGREIFGSHALMTVGSVPNTKDLGLEKIGIATTRSGHICV
DRVSRTNVAGVYAGGDCTDLFPLASVAAMQGRIAMYHALGEGVKPIRMKTVATAVFTRPEIAAVGVTQKQIESGEVIART
VMLPLQTNPRAKMRSLRHGFVKMFCRKNSGIVIGGVVVAPTASELILPIAVAVTNQLTVSDLAESFAVYPSLSGSITEAA
RQLVQHDDLQ

Sequences:

>Translated_490_residues
MGCLRYTWALDSSNRHTHTGGTVTRIVIIGGGPAGYEAALAGAKYGAEITIIEDRGLGGAAVINDCVPSKSFIAGANIKT
DLRRADDMGLNKGIGEAHLLIDALNNRVQALAYEQSSDIRASMDAHGVRVIDGRGSFDDYNPKQTVHYIKVDRADGTTET
IECDLVLIATGATPRILPDAQPDGERILTWRQIYGLTELPEHLIVVGSGVTGAEFVSAFAELGVKVTMVASRDRILPHDD
ADAADVLETVLAERGVALEKHARVDSVIRTEDGGVCVKTSDGREIFGSHALMTVGSVPNTKDLGLEKIGIATTRSGHICV
DRVSRTNVAGVYAGGDCTDLFPLASVAAMQGRIAMYHALGEGVKPIRMKTVATAVFTRPEIAAVGVTQKQIESGEVIART
VMLPLQTNPRAKMRSLRHGFVKMFCRKNSGIVIGGVVVAPTASELILPIAVAVTNQLTVSDLAESFAVYPSLSGSITEAA
RQLVQHDDLQ
>Mature_489_residues
GCLRYTWALDSSNRHTHTGGTVTRIVIIGGGPAGYEAALAGAKYGAEITIIEDRGLGGAAVINDCVPSKSFIAGANIKTD
LRRADDMGLNKGIGEAHLLIDALNNRVQALAYEQSSDIRASMDAHGVRVIDGRGSFDDYNPKQTVHYIKVDRADGTTETI
ECDLVLIATGATPRILPDAQPDGERILTWRQIYGLTELPEHLIVVGSGVTGAEFVSAFAELGVKVTMVASRDRILPHDDA
DAADVLETVLAERGVALEKHARVDSVIRTEDGGVCVKTSDGREIFGSHALMTVGSVPNTKDLGLEKIGIATTRSGHICVD
RVSRTNVAGVYAGGDCTDLFPLASVAAMQGRIAMYHALGEGVKPIRMKTVATAVFTRPEIAAVGVTQKQIESGEVIARTV
MLPLQTNPRAKMRSLRHGFVKMFCRKNSGIVIGGVVVAPTASELILPIAVAVTNQLTVSDLAESFAVYPSLSGSITEAAR
QLVQHDDLQ

Specific function: May contribute to virulence by increasing resistance to reactive oxygen intermediates. It can reduce 2,6-dimethyl-1,4- benzoquinone (DMBQ), 5-hydroxy-1,4-naphthaquinone (5-HNQ) and menadione. NAD(P)H is the physiological reductant rather than NADH [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=465, Percent_Identity=26.8817204301075, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI50301238, Length=461, Percent_Identity=27.5488069414317, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI291045266, Length=435, Percent_Identity=27.1264367816092, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI22035672, Length=441, Percent_Identity=25.6235827664399, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI148277065, Length=457, Percent_Identity=24.507658643326, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI148277071, Length=457, Percent_Identity=24.507658643326, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI33519430, Length=457, Percent_Identity=24.507658643326, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI33519428, Length=457, Percent_Identity=24.507658643326, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI33519426, Length=457, Percent_Identity=24.507658643326, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI291045268, Length=430, Percent_Identity=25.3488372093023, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1786307, Length=464, Percent_Identity=28.2327586206897, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI87082354, Length=465, Percent_Identity=26.6666666666667, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87081717, Length=461, Percent_Identity=27.765726681128, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1789915, Length=446, Percent_Identity=27.3542600896861, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32565766, Length=471, Percent_Identity=27.1762208067941, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=26.9473684210526, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71983429, Length=443, Percent_Identity=26.86230248307, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=443, Percent_Identity=26.86230248307, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71982272, Length=443, Percent_Identity=22.7990970654628, Blast_Score=83, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321091, Length=472, Percent_Identity=27.3305084745763, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6325166, Length=445, Percent_Identity=23.3707865168539, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6325240, Length=478, Percent_Identity=21.9665271966527, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21358499, Length=464, Percent_Identity=29.3103448275862, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24640553, Length=494, Percent_Identity=25.9109311740891, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24640551, Length=480, Percent_Identity=26.6666666666667, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24640549, Length=480, Percent_Identity=26.25, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI17737741, Length=478, Percent_Identity=24.4769874476987, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.6.5.2 [H]

Molecular weight: Translated: 52044; Mature: 51913

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGCLRYTWALDSSNRHTHTGGTVTRIVIIGGGPAGYEAALAGAKYGAEITIIEDRGLGGA
CCCEEEEEEECCCCCEEECCCCEEEEEEECCCCCCHHHHHCCCCCCCEEEEEECCCCCCH
AVINDCVPSKSFIAGANIKTDLRRADDMGLNKGIGEAHLLIDALNNRVQALAYEQSSDIR
HHHHHCCCCCCEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCE
ASMDAHGVRVIDGRGSFDDYNPKQTVHYIKVDRADGTTETIECDLVLIATGATPRILPDA
EECCCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCC
QPDGERILTWRQIYGLTELPEHLIVVGSGVTGAEFVSAFAELGVKVTMVASRDRILPHDD
CCCCCCEEEHHHHCCHHCCCCEEEEEECCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCC
ADAADVLETVLAERGVALEKHARVDSVIRTEDGGVCVKTSDGREIFGSHALMTVGSVPNT
CCHHHHHHHHHHHCCCCHHHHHHHHHHEEECCCCEEEECCCCHHHHCCCEEEEECCCCCC
KDLGLEKIGIATTRSGHICVDRVSRTNVAGVYAGGDCTDLFPLASVAAMQGRIAMYHALG
CCCCCCCEEEEECCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHCCEEEEEHHC
EGVKPIRMKTVATAVFTRPEIAAVGVTQKQIESGEVIARTVMLPLQTNPRAKMRSLRHGF
CCCCCEEHHHHHHHHHCCCCEEEECCCHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHH
VKMFCRKNSGIVIGGVVVAPTASELILPIAVAVTNQLTVSDLAESFAVYPSLSGSITEAA
HHHHHCCCCCEEEEEEEECCCCHHHHHHEEEHHCCCCCHHHHHHHHEECCCCCCHHHHHH
RQLVQHDDLQ
HHHHHHCCCC
>Mature Secondary Structure 
GCLRYTWALDSSNRHTHTGGTVTRIVIIGGGPAGYEAALAGAKYGAEITIIEDRGLGGA
CCEEEEEEECCCCCEEECCCCEEEEEEECCCCCCHHHHHCCCCCCCEEEEEECCCCCCH
AVINDCVPSKSFIAGANIKTDLRRADDMGLNKGIGEAHLLIDALNNRVQALAYEQSSDIR
HHHHHCCCCCCEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCE
ASMDAHGVRVIDGRGSFDDYNPKQTVHYIKVDRADGTTETIECDLVLIATGATPRILPDA
EECCCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCC
QPDGERILTWRQIYGLTELPEHLIVVGSGVTGAEFVSAFAELGVKVTMVASRDRILPHDD
CCCCCCEEEHHHHCCHHCCCCEEEEEECCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCC
ADAADVLETVLAERGVALEKHARVDSVIRTEDGGVCVKTSDGREIFGSHALMTVGSVPNT
CCHHHHHHHHHHHCCCCHHHHHHHHHHEEECCCCEEEECCCCHHHHCCCEEEEECCCCCC
KDLGLEKIGIATTRSGHICVDRVSRTNVAGVYAGGDCTDLFPLASVAAMQGRIAMYHALG
CCCCCCCEEEEECCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHCCEEEEEHHC
EGVKPIRMKTVATAVFTRPEIAAVGVTQKQIESGEVIARTVMLPLQTNPRAKMRSLRHGF
CCCCCEEHHHHHHHHHCCCCEEEECCCHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHH
VKMFCRKNSGIVIGGVVVAPTASELILPIAVAVTNQLTVSDLAESFAVYPSLSGSITEAA
HHHHHCCCCCEEEEEEEECCCCHHHHHHEEEHHCCCCCHHHHHHHHEECCCCCCHHHHHH
RQLVQHDDLQ
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]