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The map label for this gene is menE [H]

Identifier: 38233034

GI number: 38233034

Start: 384980

End: 386089

Strand: Reverse

Name: menE [H]

Synonym: DIP0420

Alternate gene names: 38233034

Gene position: 386089-384980 (Counterclockwise)

Preceding gene: 38233035

Following gene: 38233032

Centisome position: 15.51

GC content: 56.67

Gene sequence:

>1110_bases
ATGATCCTCGAACCCCTCATCGTCCCCGCATATCCCAATGAGATCCTCGATGAGCTCGAGGCCGCGATAGCTGGCGAACG
CTGCTACCTGCCGGTACCACCCAACGACCAGCTGCTTAGCCGCACCCTGAAAGCCGGACAGCCCATCGACGACGACATCG
CTCTCGTCGTAGCCACCTCCGGCTCCACAGGCACACCCAAAGGCGCACTTCTAACCGCCGCCAACCTTGTCTCATCCGCC
GACGCTACCCACCAGTACCTCGGCGGAGAAGCACAGTGGCTACTCGCTATGCCTGGCTACCACATTGCCGGAATCCAGGT
ACTTATTCGATCACTAATTGCTGGGTACGACCCCCTTATTCTCGATGTCTCCCACGGGTTCAACATTTCCGAATTCGCAC
GCATCGCCAACACTTTGGACGACACAACCTACACATCGTTGACCCCCATGCAGTTGCTCAAAGCCATGGACACCCTCGAA
GGTATTGAATCGTTACGACGTTTCGCCGCGATCCTTGTGGGCGGAGCACCCTTGCGTGCCGACGACCTCCGCGCCGCGAA
AGAACTCGGAATCAACGTTGTAACAACCTACGGTTCCTCCGAAACTTCTGGTGGCTGCGTATATAACGGTCGCCCCATCC
CTGGTGCCAAAGTTCGCGTTGTCGGCGAACGAATCCATCTCGGCGGACCAATGGTGGCGCGCGGATACCGCAACGGTGAA
GAATTCGGCGACTGGTTCATCACCTCTGACACCGGCACAATTACCGACGGCGTGCTCACAGTTACTGGACGTGTGGATAC
GATCATTGACTCAGGTGGACTGAAAATCCATCCCGAAATTCTCGAACAACAAATTCTGAGCGTCCGCGGTGTTTCAGGAG
TATGCGTTGTGGGCGTACCAGATCGACGCTTGGGACAAGCCATCGTAGCCGCATACGAAGGAAGCGCCCATCCCACCGAG
ATCATCACCGCTCTTGATGACCTGCCGCGCTGGCAATTGCCCAAAGACATCCGCCGTGTAGACCAGTTGCCACTCATTGG
GCCTGGCAAAGTAAACCGCCAAGCAGTACTCAAGATGTTCGATACTTCAATTTCGCATCGCGGCGCTTAG

Upstream 100 bases:

>100_bases
GCCTACATGACCGACGAGGCCGTAGAAGGCCGCGACTCCTTCCTTGAAAAACGCGACCCCGATTGGTCACGCTTCCCTTA
TTACTTCTAAAATTTTCAGC

Downstream 100 bases:

>100_bases
TCCGCGCCTCCACCCGCTGAGCAAAACTCTGCCCAAACATCACCCCCTCTGCCGCTGTTGTCACTTCCGTACCATCAGCC
ATGATCCAGTCCACCGCGAT

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MILEPLIVPAYPNEILDELEAAIAGERCYLPVPPNDQLLSRTLKAGQPIDDDIALVVATSGSTGTPKGALLTAANLVSSA
DATHQYLGGEAQWLLAMPGYHIAGIQVLIRSLIAGYDPLILDVSHGFNISEFARIANTLDDTTYTSLTPMQLLKAMDTLE
GIESLRRFAAILVGGAPLRADDLRAAKELGINVVTTYGSSETSGGCVYNGRPIPGAKVRVVGERIHLGGPMVARGYRNGE
EFGDWFITSDTGTITDGVLTVTGRVDTIIDSGGLKIHPEILEQQILSVRGVSGVCVVGVPDRRLGQAIVAAYEGSAHPTE
IITALDDLPRWQLPKDIRRVDQLPLIGPGKVNRQAVLKMFDTSISHRGA

Sequences:

>Translated_369_residues
MILEPLIVPAYPNEILDELEAAIAGERCYLPVPPNDQLLSRTLKAGQPIDDDIALVVATSGSTGTPKGALLTAANLVSSA
DATHQYLGGEAQWLLAMPGYHIAGIQVLIRSLIAGYDPLILDVSHGFNISEFARIANTLDDTTYTSLTPMQLLKAMDTLE
GIESLRRFAAILVGGAPLRADDLRAAKELGINVVTTYGSSETSGGCVYNGRPIPGAKVRVVGERIHLGGPMVARGYRNGE
EFGDWFITSDTGTITDGVLTVTGRVDTIIDSGGLKIHPEILEQQILSVRGVSGVCVVGVPDRRLGQAIVAAYEGSAHPTE
IITALDDLPRWQLPKDIRRVDQLPLIGPGKVNRQAVLKMFDTSISHRGA
>Mature_369_residues
MILEPLIVPAYPNEILDELEAAIAGERCYLPVPPNDQLLSRTLKAGQPIDDDIALVVATSGSTGTPKGALLTAANLVSSA
DATHQYLGGEAQWLLAMPGYHIAGIQVLIRSLIAGYDPLILDVSHGFNISEFARIANTLDDTTYTSLTPMQLLKAMDTLE
GIESLRRFAAILVGGAPLRADDLRAAKELGINVVTTYGSSETSGGCVYNGRPIPGAKVRVVGERIHLGGPMVARGYRNGE
EFGDWFITSDTGTITDGVLTVTGRVDTIIDSGGLKIHPEILEQQILSVRGVSGVCVVGVPDRRLGQAIVAAYEGSAHPTE
IITALDDLPRWQLPKDIRRVDQLPLIGPGKVNRQAVLKMFDTSISHRGA

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA). May be involved in the biosynthesis of menaquinone [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=383, Percent_Identity=26.6318537859008, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI187761343, Length=383, Percent_Identity=26.6318537859008, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1788595, Length=317, Percent_Identity=30.2839116719243, Blast_Score=118, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17558820, Length=347, Percent_Identity=26.2247838616715, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17556356, Length=218, Percent_Identity=27.5229357798165, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24653035, Length=351, Percent_Identity=24.2165242165242, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 39360; Mature: 39360

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILEPLIVPAYPNEILDELEAAIAGERCYLPVPPNDQLLSRTLKAGQPIDDDIALVVATS
CCCCCEEECCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEEEC
GSTGTPKGALLTAANLVSSADATHQYLGGEAQWLLAMPGYHIAGIQVLIRSLIAGYDPLI
CCCCCCCCHHHHHHHHHHHCHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEE
LDVSHGFNISEFARIANTLDDTTYTSLTPMQLLKAMDTLEGIESLRRFAAILVGGAPLRA
EEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
DDLRAAKELGINVVTTYGSSETSGGCVYNGRPIPGAKVRVVGERIHLGGPMVARGYRNGE
HHHHHHHHCCCEEEEECCCCCCCCCEEECCCCCCCCEEEEEEEEEECCCCCEECCCCCCH
EFGDWFITSDTGTITDGVLTVTGRVDTIIDSGGLKIHPEILEQQILSVRGVSGVCVVGVP
HHCCEEEECCCCCCCCCEEEEECCEEEEECCCCCEECHHHHHHHHHHHCCCCCEEEEECC
DRRLGQAIVAAYEGSAHPTEIITALDDLPRWQLPKDIRRVDQLPLIGPGKVNRQAVLKMF
CHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHH
DTSISHRGA
HHHHHCCCC
>Mature Secondary Structure
MILEPLIVPAYPNEILDELEAAIAGERCYLPVPPNDQLLSRTLKAGQPIDDDIALVVATS
CCCCCEEECCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEEEC
GSTGTPKGALLTAANLVSSADATHQYLGGEAQWLLAMPGYHIAGIQVLIRSLIAGYDPLI
CCCCCCCCHHHHHHHHHHHCHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEE
LDVSHGFNISEFARIANTLDDTTYTSLTPMQLLKAMDTLEGIESLRRFAAILVGGAPLRA
EEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
DDLRAAKELGINVVTTYGSSETSGGCVYNGRPIPGAKVRVVGERIHLGGPMVARGYRNGE
HHHHHHHHCCCEEEEECCCCCCCCCEEECCCCCCCCEEEEEEEEEECCCCCEECCCCCCH
EFGDWFITSDTGTITDGVLTVTGRVDTIIDSGGLKIHPEILEQQILSVRGVSGVCVVGVP
HHCCEEEECCCCCCCCCEEEEECCEEEEECCCCCEECHHHHHHHHHHHCCCCCEEEEECC
DRRLGQAIVAAYEGSAHPTEIITALDDLPRWQLPKDIRRVDQLPLIGPGKVNRQAVLKMF
CHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHH
DTSISHRGA
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]