BacMap

Definition	Corynebacterium diphtheriae NCTC 13129 chromosome, complete genome.
Accession	NC_002935
Length	2,488,635

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The map label for this gene is ape2 [H]

Identifier: 38232979

GI number: 38232979

Start: 329691

End: 331043

Strand: Reverse

Name: ape2 [H]

Synonym: DIP0363

Alternate gene names: 38232979

Gene position: 331043-329691 (Counterclockwise)

Preceding gene: 38232980

Following gene: 38232978

Centisome position: 13.3

GC content: 56.76

Gene sequence:

>1353_bases
ATGAGCCGGCTTCGCTCTACGCCCATTCCTGGTACGCGGGACTCCTATACGCAGGTGGACTTCAACTTAGGGTTTCACGT
GCGGCATTACGACGTCTCCTTGGACTATGTGGTTGCACCGAATCGTTTGAAGGCTGTGGTCACGCTCTCGATGGATACTT
ATCAGCCGCTGCGCAGCCTTACGCTGGATCTTTCCGACGCGTTGACGGTGAAGTCGGTGACAGCACAAGGTACGTCGGCG
GTGGCTGTGAGGGTGCAGAAGTTCCGACACCGCAACCATAAGCTGCGGATTTCTTTTACCGAAGAGATCCCCGTGGACCA
GGAATTTAAGATCACCGTGACCTATTCCGGTAATCCGCGCCCGATTCGGAGCACATGGGGGTTGATCGGCTGGGAGGAGC
TGAGCAACGGTTCCTTGGTGGCCAGCCAGCCCAATGGTGCGCGTTCGTGGTTGCCGTGTGATGACACTCCCGATGAAAAG
GCGCTGTACACCATGAGCATTACGTGCGATTCGCCGTACACGGTGGTGGCTAATGGAGAGTTGCTGGGCACCACGAGGCA
TGGTTCGCGCACACAATGGCGCTATCAAACGCAGCATCCGATGGCGAGCTATTTGGCAACAGTGCAGGTTGGGCGATATG
AGCGCTATGAGCTTGGCGCCGCAGGTGAGGTTCCCGTAATCGCGTATGCTCCGCCGGCGTTGGCGGCTGGGGTGCGCCAT
GATTTTTCGGACCAGACTGCGATGCTGGAGCTGTTTTCTCAGTTGTTTGGCCCCTACCCTTTTGACCAGTACTCGGTGGT
TGTAACCGAGGATGATCTTGAGATTCCGTTGGAGGCTCAGGGGTTGTCTATTTTTGGTGCGAATCATGCTGGGGGTACCA
AGCAGTGGGAACGGCTGATTGCCCATGAGTTGGCGCATCAGTGGTTTGGTAATTCTTTGGGTCTTGCGCAGTGGGATGAT
ATTTGGCTGAATGAGGGCTTTGCGTGCTATGCGGAGTGGTTGTGGTTTGAGCATTCGGCGGGGATTCCGGCGGCGGTAAC
GGCGTCCCAGCATTATGAACGTTTAGCGCAGCTTCCTGCAGATATTGTGGTGGCAGCCCCTGGTGCACGCGATATGTTTG
ATGATCGTGTGTATAAGCGTGGTGCGTTGACGCTGCATGCGCTTCGCACGCTGCTTGGCAGCGCAGCTTTCTTCCGCGCC
ATTGCACGTTATGTTGCGGCGGGTCGGCATTGCGTTGTGGAGCCGGTGGATTTACGCCGCGAGTTGCTCAAGGAAGTGGA
TTCTTCGGCGCAGCTGGATGCGTTGTGGCATGCGTGGTTGTATGAGACTGCGTTGCCGGAGTGCCCACGGTAA

Upstream 100 bases:

>100_bases
ATTCGGAGGGCGGCCATGCGGGCGCTGCGGATAATGCGCAGGTGGCGGCGTCGGAGGCCATGATTTATACGTGGCTGCTT
GACGCATTGGGAGGCGTACG

Downstream 100 bases:

>100_bases
CATCGATGGCTGTGAAACGTATGCTGGTCACCGGCGGCGCCGGTTTTATTGGTTCGAATTTTGTCAGGCGTGTTCTTGCT
ACCCGTCCGGAGTATCGGGT

Product: putative metallopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSRLRSTPIPGTRDSYTQVDFNLGFHVRHYDVSLDYVVAPNRLKAVVTLSMDTYQPLRSLTLDLSDALTVKSVTAQGTSA
VAVRVQKFRHRNHKLRISFTEEIPVDQEFKITVTYSGNPRPIRSTWGLIGWEELSNGSLVASQPNGARSWLPCDDTPDEK
ALYTMSITCDSPYTVVANGELLGTTRHGSRTQWRYQTQHPMASYLATVQVGRYERYELGAAGEVPVIAYAPPALAAGVRH
DFSDQTAMLELFSQLFGPYPFDQYSVVVTEDDLEIPLEAQGLSIFGANHAGGTKQWERLIAHELAHQWFGNSLGLAQWDD
IWLNEGFACYAEWLWFEHSAGIPAAVTASQHYERLAQLPADIVVAAPGARDMFDDRVYKRGALTLHALRTLLGSAAFFRA
IARYVAAGRHCVVEPVDLRRELLKEVDSSAQLDALWHAWLYETALPECPR

Sequences:

>Translated_450_residues
MSRLRSTPIPGTRDSYTQVDFNLGFHVRHYDVSLDYVVAPNRLKAVVTLSMDTYQPLRSLTLDLSDALTVKSVTAQGTSA
VAVRVQKFRHRNHKLRISFTEEIPVDQEFKITVTYSGNPRPIRSTWGLIGWEELSNGSLVASQPNGARSWLPCDDTPDEK
ALYTMSITCDSPYTVVANGELLGTTRHGSRTQWRYQTQHPMASYLATVQVGRYERYELGAAGEVPVIAYAPPALAAGVRH
DFSDQTAMLELFSQLFGPYPFDQYSVVVTEDDLEIPLEAQGLSIFGANHAGGTKQWERLIAHELAHQWFGNSLGLAQWDD
IWLNEGFACYAEWLWFEHSAGIPAAVTASQHYERLAQLPADIVVAAPGARDMFDDRVYKRGALTLHALRTLLGSAAFFRA
IARYVAAGRHCVVEPVDLRRELLKEVDSSAQLDALWHAWLYETALPECPR
>Mature_449_residues
SRLRSTPIPGTRDSYTQVDFNLGFHVRHYDVSLDYVVAPNRLKAVVTLSMDTYQPLRSLTLDLSDALTVKSVTAQGTSAV
AVRVQKFRHRNHKLRISFTEEIPVDQEFKITVTYSGNPRPIRSTWGLIGWEELSNGSLVASQPNGARSWLPCDDTPDEKA
LYTMSITCDSPYTVVANGELLGTTRHGSRTQWRYQTQHPMASYLATVQVGRYERYELGAAGEVPVIAYAPPALAAGVRHD
FSDQTAMLELFSQLFGPYPFDQYSVVVTEDDLEIPLEAQGLSIFGANHAGGTKQWERLIAHELAHQWFGNSLGLAQWDDI
WLNEGFACYAEWLWFEHSAGIPAAVTASQHYERLAQLPADIVVAAPGARDMFDDRVYKRGALTLHALRTLLGSAAFFRAI
ARYVAAGRHCVVEPVDLRRELLKEVDSSAQLDALWHAWLYETALPECPR

Specific function: Preferentially acts as a leucyl-aminopeptidase, although it also has activity against other substrates [H]

COG id: COG0308

COG function: function code E; Aminopeptidase N

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M1 family [H]

Homologues:

Organism=Homo sapiens, GI61742775, Length=447, Percent_Identity=23.2662192393736, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI61742777, Length=447, Percent_Identity=23.2662192393736, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI4505029, Length=422, Percent_Identity=25.3554502369668, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI94818891, Length=298, Percent_Identity=26.1744966442953, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI94818901, Length=298, Percent_Identity=26.1744966442953, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI194306629, Length=290, Percent_Identity=26.2068965517241, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI11641261, Length=290, Percent_Identity=26.2068965517241, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI158937236, Length=337, Percent_Identity=24.3323442136499, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI132814467, Length=414, Percent_Identity=22.2222222222222, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI310123622, Length=297, Percent_Identity=24.9158249158249, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI310133497, Length=297, Percent_Identity=24.5791245791246, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI157266300, Length=292, Percent_Identity=26.7123287671233, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI40316915, Length=259, Percent_Identity=25.4826254826255, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI194239713, Length=346, Percent_Identity=23.9884393063584, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI4507347, Length=208, Percent_Identity=25.9615384615385, Blast_Score=70, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI193206926, Length=371, Percent_Identity=24.5283018867925, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI193206928, Length=371, Percent_Identity=24.5283018867925, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71984339, Length=471, Percent_Identity=23.7791932059448, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71984353, Length=484, Percent_Identity=23.7603305785124, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17569221, Length=196, Percent_Identity=29.0816326530612, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI133903840, Length=303, Percent_Identity=27.3927392739274, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17565628, Length=310, Percent_Identity=25.4838709677419, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17569225, Length=318, Percent_Identity=24.5283018867925, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI115533278, Length=299, Percent_Identity=26.4214046822742, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI115533276, Length=299, Percent_Identity=26.4214046822742, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17544504, Length=436, Percent_Identity=22.4770642201835, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71989076, Length=201, Percent_Identity=26.3681592039801, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71990873, Length=314, Percent_Identity=27.3885350318471, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71989071, Length=201, Percent_Identity=26.3681592039801, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI9755335, Length=331, Percent_Identity=23.2628398791541, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322054, Length=349, Percent_Identity=23.7822349570201, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324283, Length=330, Percent_Identity=24.5454545454545, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321837, Length=292, Percent_Identity=23.2876712328767, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24646516, Length=334, Percent_Identity=24.251497005988, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24646514, Length=334, Percent_Identity=24.251497005988, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24646518, Length=334, Percent_Identity=24.251497005988, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585062, Length=321, Percent_Identity=25.8566978193146, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585064, Length=321, Percent_Identity=25.8566978193146, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585066, Length=321, Percent_Identity=25.8566978193146, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI45550350, Length=321, Percent_Identity=25.8566978193146, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24646512, Length=344, Percent_Identity=25, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24646510, Length=344, Percent_Identity=25, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI221379089, Length=486, Percent_Identity=20.9876543209877, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24651025, Length=347, Percent_Identity=23.9193083573487, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24651023, Length=347, Percent_Identity=23.9193083573487, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24651021, Length=347, Percent_Identity=23.9193083573487, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45550850, Length=336, Percent_Identity=23.8095238095238, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24650973, Length=336, Percent_Identity=23.8095238095238, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24655274, Length=412, Percent_Identity=23.0582524271845, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24655260, Length=412, Percent_Identity=23.0582524271845, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24655265, Length=412, Percent_Identity=23.0582524271845, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24655268, Length=412, Percent_Identity=23.0582524271845, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24655257, Length=412, Percent_Identity=23.0582524271845, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24655252, Length=412, Percent_Identity=23.0582524271845, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21358341, Length=336, Percent_Identity=22.6190476190476, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571901, Length=289, Percent_Identity=25.6055363321799, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI161078673, Length=289, Percent_Identity=25.6055363321799, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24651016, Length=283, Percent_Identity=23.6749116607774, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24648784, Length=346, Percent_Identity=23.121387283237, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281362224, Length=413, Percent_Identity=22.7602905569007, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI221458256, Length=413, Percent_Identity=22.7602905569007, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221330574, Length=348, Percent_Identity=22.7011494252874, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281362221, Length=284, Percent_Identity=23.5915492957746, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24641062, Length=374, Percent_Identity=23.5294117647059, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001930
- InterPro:   IPR014782 [H]

Pfam domain/function: PF01433 Peptidase_M1 [H]

EC number: =3.4.11.1 [H]

Molecular weight: Translated: 50329; Mature: 50198

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLRSTPIPGTRDSYTQVDFNLGFHVRHYDVSLDYVVAPNRLKAVVTLSMDTYQPLRSL
CCCCCCCCCCCCCCCCEEEEEECCEEEEEEEEEEEEEECCCCEEEEEEEECCHHHHHHHH
TLDLSDALTVKSVTAQGTSAVAVRVQKFRHRNHKLRISFTEEIPVDQEFKITVTYSGNPR
EECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEEECCCCC
PIRSTWGLIGWEELSNGSLVASQPNGARSWLPCDDTPDEKALYTMSITCDSPYTVVANGE
CCHHHCCCCCHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEECCE
LLGTTRHGSRTQWRYQTQHPMASYLATVQVGRYERYELGAAGEVPVIAYAPPALAAGVRH
EEEECCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEECCCCCCCCEEEECCCHHHHHCCC
DFSDQTAMLELFSQLFGPYPFDQYSVVVTEDDLEIPLEAQGLSIFGANHAGGTKQWERLI
CCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEECCEEEEECCCCCCHHHHHHHH
AHELAHQWFGNSLGLAQWDDIWLNEGFACYAEWLWFEHSAGIPAAVTASQHYERLAQLPA
HHHHHHHHCCCCCCCCCCCCHHHCCCCEEEEEEHEEECCCCCCEEEHHHHHHHHHHHCCC
DIVVAAPGARDMFDDRVYKRGALTLHALRTLLGSAAFFRAIARYVAAGRHCVVEPVDLRR
CEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHH
ELLKEVDSSAQLDALWHAWLYETALPECPR
HHHHHCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SRLRSTPIPGTRDSYTQVDFNLGFHVRHYDVSLDYVVAPNRLKAVVTLSMDTYQPLRSL
CCCCCCCCCCCCCCCEEEEEECCEEEEEEEEEEEEEECCCCEEEEEEEECCHHHHHHHH
TLDLSDALTVKSVTAQGTSAVAVRVQKFRHRNHKLRISFTEEIPVDQEFKITVTYSGNPR
EECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEEECCCCC
PIRSTWGLIGWEELSNGSLVASQPNGARSWLPCDDTPDEKALYTMSITCDSPYTVVANGE
CCHHHCCCCCHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEECCE
LLGTTRHGSRTQWRYQTQHPMASYLATVQVGRYERYELGAAGEVPVIAYAPPALAAGVRH
EEEECCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEECCCCCCCCEEEECCCHHHHHCCC
DFSDQTAMLELFSQLFGPYPFDQYSVVVTEDDLEIPLEAQGLSIFGANHAGGTKQWERLI
CCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEECCEEEEECCCCCCHHHHHHHH
AHELAHQWFGNSLGLAQWDDIWLNEGFACYAEWLWFEHSAGIPAAVTASQHYERLAQLPA
HHHHHHHHCCCCCCCCCCCCHHHCCCCEEEEEEHEEECCCCCCEEEHHHHHHHHHHHCCC
DIVVAAPGARDMFDDRVYKRGALTLHALRTLLGSAAFFRAIARYVAAGRHCVVEPVDLRR
CEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHH
ELLKEVDSSAQLDALWHAWLYETALPECPR
HHHHHCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9723917; 8899719; 11427726 [H]