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The map label for this gene is ptrB [C]

Identifier: 38232980

GI number: 38232980

Start: 331040

End: 333034

Strand: Reverse

Name: ptrB [C]

Synonym: DIP0364

Alternate gene names: 38232980

Gene position: 333034-331040 (Counterclockwise)

Preceding gene: 38232981

Following gene: 38232979

Centisome position: 13.38

GC content: 57.84

Gene sequence:

>1995_bases
ATGCACCACCCCCCTGTTGAGGAGCTAGAGCTGATCGATTCCCCCGCCGCTCTTGAGTGGGCGCGCGAGTGGTCGCAGGC
TACGCAGGAGAGGTGGAGCAACGATGCACTCCAGCGCAGTATTCGTGAGGCGTTGGATACGGACGCACGTATTCCTTATG
TGACGAGGCGGGGCGAATATCTGTATAACTTTTGGCGGGATCGCGACCACCCCCGTGGGGTGTGGCGACGTACTACGTTG
GAGAGTTTTACCAGCGATTCCCCACAATGGCAGGTGCTTATCGACGTCGACCGCCTTGCTGCTTCTGAGGAAGAAAGTTG
GGTATGGAAGGGCGCTCATGTTCGTCCGCATCATTTTGATCGTGCGTTGATTAGGTTGAGCCGTGGTGGCGCCGACGCGG
TGGAAATCCGCGAGTTTGATCTTGATAGTGGCAGTTTTGTTGAAGATCACGCGTTTGCGGTTCCGGAGGCCAAGACTCAG
GTGTGCTGGGTGGATCGCGATACTGTGCTGGTAGGAACGGATATGGGGGCCGGCAGTTTGACGGAGTCGGGCTATCCGGC
GCGGGTTCATGTGTGGCGGCGTGGCACGAATCTTGCTGAGGCTGAGGAGTTTTTCTGCGGCCTTAGCTCGGATTTGGCGG
TGTCTGCGTGGGCGGAGACTGTCCCTGGTTTTGAGCGGTTGTTTGTGCGTCGGGCGCTGGATTTTTATCGTTCGCGGACG
TTTATTCAGCGTGATGGTCATCTGCAGATTATTGAGGTGCCGGAGGATTGCACGGTGGTGGTCCATCGTGAGTGGATGTA
TGTACTGCCACGTGAGGAGTACGCCGGTATTGCTTCTGGTGGTGTGGGCGCTATTTTGTTTGAGGATTTCTTGGCGGGTC
GCAGGGATTTCTTCACGGTGTTTAGTCCTACGCCGCAATCGAGTGTGCAGGATCTTACGTTGACGGCCTCCTATGTGGTG
TTGACGATTTTGGAGGATGTGACCAGCCGGATTGAGGTTGTTGATCGCGGGGCACCGGGGTCGGCGTCGCGACGCATCAA
TGTCGGCGATATGGTTACCGCGCGTGTGGTGGCGGCGGATTCGGAATCTGATGAGCTGTGGTTGGGGGCATCGTCGTTTA
CGCAACCAGATACGTTGTATCGCGTTGAGCTTGGCCAACACACTACTCCTGAGGTGGCCAAGCAGGCTCCTGCGCTGTTT
GATGCTTCTCGTATGGAGACGCGGCAGCATTGGGCAACGTCGGCAGATGGCACCAAGATTCCGTATTTTATCGTGGGAGA
TTTTTCTCAAGGTCCGCGTCCTACGTTGGTGGGCGGCTATGGCGGCTTTGAGGTTTCGCTGGTGCCTGGGTATTCGTCGA
TACGCGGTCGGGCGTGGCTGAGCAAGGGAAACTATTTTGTACAACCTAATTTGCGCGGTGGTGGCGAGTTCGGTCCGCAC
TGGCATGAAAGTGTGATTAGGCGTCATCGCATGAAGATTTATGAGGATCATCAAGCGGTTTTAGAGGATCTGCGGCAGCG
CGGGTATGCCTCGCAGATTGCGGTGCGCGGCGGTTCCAATGGTGGGTTGCTCACGTCGGTGGCGTTAACGCGTTATCCGG
AGGCGATTGATGCTGCGGTGATTCAGGTGCCGCTGACCGACATGTTGCGGTATCACACGTGGTCGGCGGGTGCGTCGTGG
ATGGCGGAGTATGGGGATCCCGATGATCCGGCGGAGCGGGCGGTATTGGAAAGTTATTCGCCCTTGCACCATGTGGCAGC
TCGTGCGCAGGTGGCGTACCCGCCGGCGTTGGTGACTACGTCGACACGCGATGATCGTGTCCACCCCGCTCATGCGCGTT
TGTTTGCCCGTGCTTTGGAGGTTGCTGGGCAGGCTGTGGACTATTGGGAGAATTCGGAGGGCGGCCATGCGGGCGCTGCG
GATAATGCGCAGGTGGCGGCGTCGGAGGCCATGATTTATACGTGGCTGCTTGACGCATTGGGAGGCGTACGATGA

Upstream 100 bases:

>100_bases
TTTAACAAGTAAATCTCTTCGCTTTACTTCCTCGCTGATACGCTTTGGGTGCGTTATCAGCGAGGTTTTTTATTACCCCC
AAGGAAAGGACACGCCGGCG

Downstream 100 bases:

>100_bases
GCCGGCTTCGCTCTACGCCCATTCCTGGTACGCGGGACTCCTATACGCAGGTGGACTTCAACTTAGGGTTTCACGTGCGG
CATTACGACGTCTCCTTGGA

Product: putative prolyl oligopeptidase family protein

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 664; Mature: 664

Protein sequence:

>664_residues
MHHPPVEELELIDSPAALEWAREWSQATQERWSNDALQRSIREALDTDARIPYVTRRGEYLYNFWRDRDHPRGVWRRTTL
ESFTSDSPQWQVLIDVDRLAASEEESWVWKGAHVRPHHFDRALIRLSRGGADAVEIREFDLDSGSFVEDHAFAVPEAKTQ
VCWVDRDTVLVGTDMGAGSLTESGYPARVHVWRRGTNLAEAEEFFCGLSSDLAVSAWAETVPGFERLFVRRALDFYRSRT
FIQRDGHLQIIEVPEDCTVVVHREWMYVLPREEYAGIASGGVGAILFEDFLAGRRDFFTVFSPTPQSSVQDLTLTASYVV
LTILEDVTSRIEVVDRGAPGSASRRINVGDMVTARVVAADSESDELWLGASSFTQPDTLYRVELGQHTTPEVAKQAPALF
DASRMETRQHWATSADGTKIPYFIVGDFSQGPRPTLVGGYGGFEVSLVPGYSSIRGRAWLSKGNYFVQPNLRGGGEFGPH
WHESVIRRHRMKIYEDHQAVLEDLRQRGYASQIAVRGGSNGGLLTSVALTRYPEAIDAAVIQVPLTDMLRYHTWSAGASW
MAEYGDPDDPAERAVLESYSPLHHVAARAQVAYPPALVTTSTRDDRVHPAHARLFARALEVAGQAVDYWENSEGGHAGAA
DNAQVAASEAMIYTWLLDALGGVR

Sequences:

>Translated_664_residues
MHHPPVEELELIDSPAALEWAREWSQATQERWSNDALQRSIREALDTDARIPYVTRRGEYLYNFWRDRDHPRGVWRRTTL
ESFTSDSPQWQVLIDVDRLAASEEESWVWKGAHVRPHHFDRALIRLSRGGADAVEIREFDLDSGSFVEDHAFAVPEAKTQ
VCWVDRDTVLVGTDMGAGSLTESGYPARVHVWRRGTNLAEAEEFFCGLSSDLAVSAWAETVPGFERLFVRRALDFYRSRT
FIQRDGHLQIIEVPEDCTVVVHREWMYVLPREEYAGIASGGVGAILFEDFLAGRRDFFTVFSPTPQSSVQDLTLTASYVV
LTILEDVTSRIEVVDRGAPGSASRRINVGDMVTARVVAADSESDELWLGASSFTQPDTLYRVELGQHTTPEVAKQAPALF
DASRMETRQHWATSADGTKIPYFIVGDFSQGPRPTLVGGYGGFEVSLVPGYSSIRGRAWLSKGNYFVQPNLRGGGEFGPH
WHESVIRRHRMKIYEDHQAVLEDLRQRGYASQIAVRGGSNGGLLTSVALTRYPEAIDAAVIQVPLTDMLRYHTWSAGASW
MAEYGDPDDPAERAVLESYSPLHHVAARAQVAYPPALVTTSTRDDRVHPAHARLFARALEVAGQAVDYWENSEGGHAGAA
DNAQVAASEAMIYTWLLDALGGVR
>Mature_664_residues
MHHPPVEELELIDSPAALEWAREWSQATQERWSNDALQRSIREALDTDARIPYVTRRGEYLYNFWRDRDHPRGVWRRTTL
ESFTSDSPQWQVLIDVDRLAASEEESWVWKGAHVRPHHFDRALIRLSRGGADAVEIREFDLDSGSFVEDHAFAVPEAKTQ
VCWVDRDTVLVGTDMGAGSLTESGYPARVHVWRRGTNLAEAEEFFCGLSSDLAVSAWAETVPGFERLFVRRALDFYRSRT
FIQRDGHLQIIEVPEDCTVVVHREWMYVLPREEYAGIASGGVGAILFEDFLAGRRDFFTVFSPTPQSSVQDLTLTASYVV
LTILEDVTSRIEVVDRGAPGSASRRINVGDMVTARVVAADSESDELWLGASSFTQPDTLYRVELGQHTTPEVAKQAPALF
DASRMETRQHWATSADGTKIPYFIVGDFSQGPRPTLVGGYGGFEVSLVPGYSSIRGRAWLSKGNYFVQPNLRGGGEFGPH
WHESVIRRHRMKIYEDHQAVLEDLRQRGYASQIAVRGGSNGGLLTSVALTRYPEAIDAAVIQVPLTDMLRYHTWSAGASW
MAEYGDPDDPAERAVLESYSPLHHVAARAQVAYPPALVTTSTRDDRVHPAHARLFARALEVAGQAVDYWENSEGGHAGAA
DNAQVAASEAMIYTWLLDALGGVR

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=364, Percent_Identity=29.9450549450549, Blast_Score=168, Evalue=1e-41,
Organism=Escherichia coli, GI1788150, Length=309, Percent_Identity=28.4789644012945, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24583414, Length=361, Percent_Identity=30.7479224376731, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI221510989, Length=334, Percent_Identity=32.3353293413174, Blast_Score=172, Evalue=8e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 74104; Mature: 74104

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHPPVEELELIDSPAALEWAREWSQATQERWSNDALQRSIREALDTDARIPYVTRRGEY
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCEEECCCHH
LYNFWRDRDHPRGVWRRTTLESFTSDSPQWQVLIDVDRLAASEEESWVWKGAHVRPHHFD
HHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCCEEEECCCCCCHHHH
RALIRLSRGGADAVEIREFDLDSGSFVEDHAFAVPEAKTQVCWVDRDTVLVGTDMGAGSL
HHHHHHHCCCCCEEEEEEEECCCCCEECCCCEECCCCCCEEEEEECCEEEEECCCCCCCC
TESGYPARVHVWRRGTNLAEAEEFFCGLSSDLAVSAWAETVPGFERLFVRRALDFYRSRT
CCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCH
FIQRDGHLQIIEVPEDCTVVVHREWMYVLPREEYAGIASGGVGAILFEDFLAGRRDFFTV
HEECCCCEEEEECCCCCEEEEEECEEEEECCHHHCCCCCCCCHHHHHHHHHCCCCCEEEE
FSPTPQSSVQDLTLTASYVVLTILEDVTSRIEVVDRGAPGSASRRINVGDMVTARVVAAD
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEEEEEC
SESDELWLGASSFTQPDTLYRVELGQHTTPEVAKQAPALFDASRMETRQHWATSADGTKI
CCCCCEEECCCCCCCCCEEEEEECCCCCCHHHHHHCCHHHCHHHHHHHHHHCCCCCCCCC
PYFIVGDFSQGPRPTLVGGYGGFEVSLVPGYSSIRGRAWLSKGNYFVQPNLRGGGEFGPH
CEEEEECCCCCCCCCEEECCCCEEEEEECCCHHHCCCEEEECCCEEEECCCCCCCCCCCH
WHESVIRRHRMKIYEDHQAVLEDLRQRGYASQIAVRGGSNGGLLTSVALTRYPEAIDAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCHHHHHHE
IQVPLTDMLRYHTWSAGASWMAEYGDPDDPAERAVLESYSPLHHVAARAQVAYPPALVTT
EEECHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEE
STRDDRVHPAHARLFARALEVAGQAVDYWENSEGGHAGAADNAQVAASEAMIYTWLLDAL
CCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GGVR
CCCC
>Mature Secondary Structure
MHHPPVEELELIDSPAALEWAREWSQATQERWSNDALQRSIREALDTDARIPYVTRRGEY
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCEEECCCHH
LYNFWRDRDHPRGVWRRTTLESFTSDSPQWQVLIDVDRLAASEEESWVWKGAHVRPHHFD
HHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCCEEEECCCCCCHHHH
RALIRLSRGGADAVEIREFDLDSGSFVEDHAFAVPEAKTQVCWVDRDTVLVGTDMGAGSL
HHHHHHHCCCCCEEEEEEEECCCCCEECCCCEECCCCCCEEEEEECCEEEEECCCCCCCC
TESGYPARVHVWRRGTNLAEAEEFFCGLSSDLAVSAWAETVPGFERLFVRRALDFYRSRT
CCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCH
FIQRDGHLQIIEVPEDCTVVVHREWMYVLPREEYAGIASGGVGAILFEDFLAGRRDFFTV
HEECCCCEEEEECCCCCEEEEEECEEEEECCHHHCCCCCCCCHHHHHHHHHCCCCCEEEE
FSPTPQSSVQDLTLTASYVVLTILEDVTSRIEVVDRGAPGSASRRINVGDMVTARVVAAD
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEEEEEC
SESDELWLGASSFTQPDTLYRVELGQHTTPEVAKQAPALFDASRMETRQHWATSADGTKI
CCCCCEEECCCCCCCCCEEEEEECCCCCCHHHHHHCCHHHCHHHHHHHHHHCCCCCCCCC
PYFIVGDFSQGPRPTLVGGYGGFEVSLVPGYSSIRGRAWLSKGNYFVQPNLRGGGEFGPH
CEEEEECCCCCCCCCEEECCCCEEEEEECCCHHHCCCEEEECCCEEEECCCCCCCCCCCH
WHESVIRRHRMKIYEDHQAVLEDLRQRGYASQIAVRGGSNGGLLTSVALTRYPEAIDAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCHHHHHHE
IQVPLTDMLRYHTWSAGASWMAEYGDPDDPAERAVLESYSPLHHVAARAQVAYPPALVTT
EEECHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEE
STRDDRVHPAHARLFARALEVAGQAVDYWENSEGGHAGAADNAQVAASEAMIYTWLLDAL
CCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GGVR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.6 {N-benzoyl-Arg} 0.92 {benzoyl-Lys} 0.23 {tosyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.47 {tosyl-Lys-methyl} 80 {acetyl-tyrosine} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]