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The map label for this gene is rfbA [H]

Identifier: 38232976

GI number: 38232976

Start: 326409

End: 327281

Strand: Reverse

Name: rfbA [H]

Synonym: DIP0360

Alternate gene names: 38232976

Gene position: 327281-326409 (Counterclockwise)

Preceding gene: 38232977

Following gene: 38232974

Centisome position: 13.15

GC content: 56.47

Gene sequence:

>873_bases
ATGAAGGGCATCATTCTCGCCGGCGGTTCTGGCACTCGTTTGCACCCCATTACGTTGGGGATTTCTAAGCAGCTTATGCC
GATCTACGACAAACCAATGATCTACTACCCGCTGACCACGTTGATCCAAGCGGGCATTTGCGAGATCTTGATCATCACCA
CCCCCGAGGATCAGGCAGCGTTTCAACGCCTGCTTGGCGACGGCTCCTCCTGGGGCATTGAGCTTTCCTACGCAGTGCAG
CCCCGCCCTGATGGCCTAGCACAGGCGTTTATTATCGGCCGCGATTTCATTGGCAACGACAGCGTTGCTCTAGCCCTAGG
CGACAACATCTTTGACGGCCCCGCCCTTGGTCAAGCACTCAAGGGGTGCCATAGTCCTGACGGCGGCACCGTGTTCGCCT
ATGAGGTATCCGACCCCAGTCGCTATGGTGTGGTCACCTTTGATGCCACAGGAACCGCATTGTCCATCGAAGAAAAACCC
GCCCACCCACGTTCCAACTATGCGGTGGTGGGACTGTACTTCTACAACAACGACGTCGTCCGCATCGCGGAAACAACCAC
ACCCAGCGCACGCGGCGAGCTAGAAATCACCGCCATTAACGACGCCTACCTACAGCAAGGCAAGCTCCATGTGCGCAAAC
TCGAACGCGGCGACGTGTGGCTCGACACCGGAACCATCGACTCGATGAGCGAAGCCGCCTCCTACGTAGAAGTACTACAA
AAACGCACCGGCGTTATCATCGGATCCCCAGAAGTAGCCGCATGGAAAGCAGGCCTTATCACCGACACCCAGCTAGAAGC
ACTCGCACAGCCACTCAAAAAATCCGGCTACGGGAACTACCTACTACGCACAATAAGATCCGCAATACCCTGA

Upstream 100 bases:

>100_bases
CGACGTTCAACTTGGACAAGATTGTGGCCACGGGTTTTCAACCGCGCCCATGGCGGGATGCTGTGCGGGATTACTTGGGC
ACCAAGTAGGCTTAGGATCC

Downstream 100 bases:

>100_bases
TGCCCGCGCTCATTCACATGAACAGGCAGATGCGTAGACGCACCCGCAACATCCACCACACTGGAAATGTAACGCTGCTC
ATCGGGTGCACACATGGAAT

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: dTDP-glucose pyrophosphorylase; dTDP-glucose synthase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MKGIILAGGSGTRLHPITLGISKQLMPIYDKPMIYYPLTTLIQAGICEILIITTPEDQAAFQRLLGDGSSWGIELSYAVQ
PRPDGLAQAFIIGRDFIGNDSVALALGDNIFDGPALGQALKGCHSPDGGTVFAYEVSDPSRYGVVTFDATGTALSIEEKP
AHPRSNYAVVGLYFYNNDVVRIAETTTPSARGELEITAINDAYLQQGKLHVRKLERGDVWLDTGTIDSMSEAASYVEVLQ
KRTGVIIGSPEVAAWKAGLITDTQLEALAQPLKKSGYGNYLLRTIRSAIP

Sequences:

>Translated_290_residues
MKGIILAGGSGTRLHPITLGISKQLMPIYDKPMIYYPLTTLIQAGICEILIITTPEDQAAFQRLLGDGSSWGIELSYAVQ
PRPDGLAQAFIIGRDFIGNDSVALALGDNIFDGPALGQALKGCHSPDGGTVFAYEVSDPSRYGVVTFDATGTALSIEEKP
AHPRSNYAVVGLYFYNNDVVRIAETTTPSARGELEITAINDAYLQQGKLHVRKLERGDVWLDTGTIDSMSEAASYVEVLQ
KRTGVIIGSPEVAAWKAGLITDTQLEALAQPLKKSGYGNYLLRTIRSAIP
>Mature_290_residues
MKGIILAGGSGTRLHPITLGISKQLMPIYDKPMIYYPLTTLIQAGICEILIITTPEDQAAFQRLLGDGSSWGIELSYAVQ
PRPDGLAQAFIIGRDFIGNDSVALALGDNIFDGPALGQALKGCHSPDGGTVFAYEVSDPSRYGVVTFDATGTALSIEEKP
AHPRSNYAVVGLYFYNNDVVRIAETTTPSARGELEITAINDAYLQQGKLHVRKLERGDVWLDTGTIDSMSEAASYVEVLQ
KRTGVIIGSPEVAAWKAGLITDTQLEALAQPLKKSGYGNYLLRTIRSAIP

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis. Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-N-acetylglucosamine-L-rhamnose disaccharide,

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=252, Percent_Identity=27.7777777777778, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI11761619, Length=252, Percent_Identity=27.7777777777778, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788351, Length=288, Percent_Identity=58.6805555555556, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI1790224, Length=288, Percent_Identity=58.3333333333333, Blast_Score=337, Evalue=6e-94,
Organism=Escherichia coli, GI1788355, Length=236, Percent_Identity=26.6949152542373, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI133931050, Length=183, Percent_Identity=27.8688524590164, Blast_Score=81, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6320148, Length=249, Percent_Identity=28.9156626506024, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21355443, Length=246, Percent_Identity=27.2357723577236, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24644084, Length=246, Percent_Identity=27.2357723577236, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 31204; Mature: 31204

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIILAGGSGTRLHPITLGISKQLMPIYDKPMIYYPLTTLIQAGICEILIITTPEDQAA
CCCEEEECCCCCEEEEEEECCCCCCCCCCCCCEEEECHHHHHHCCCEEEEEEECCCHHHH
FQRLLGDGSSWGIELSYAVQPRPDGLAQAFIIGRDFIGNDSVALALGDNIFDGPALGQAL
HHHHHCCCCCCCEEEEEEECCCCCCCEEEEEEECEECCCCCEEEEECCCCCCCHHHHHHH
KGCHSPDGGTVFAYEVSDPSRYGVVTFDATGTALSIEEKPAHPRSNYAVVGLYFYNNDVV
HCCCCCCCCEEEEEECCCCCCEEEEEECCCCCEEEECCCCCCCCCCEEEEEEEEECCCEE
RIAETTTPSARGELEITAINDAYLQQGKLHVRKLERGDVWLDTGTIDSMSEAASYVEVLQ
EEEECCCCCCCCEEEEEEECHHHHHCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHH
KRTGVIIGSPEVAAWKAGLITDTQLEALAQPLKKSGYGNYLLRTIRSAIP
HHCCEEEECCCHHEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKGIILAGGSGTRLHPITLGISKQLMPIYDKPMIYYPLTTLIQAGICEILIITTPEDQAA
CCCEEEECCCCCEEEEEEECCCCCCCCCCCCCEEEECHHHHHHCCCEEEEEEECCCHHHH
FQRLLGDGSSWGIELSYAVQPRPDGLAQAFIIGRDFIGNDSVALALGDNIFDGPALGQAL
HHHHHCCCCCCCEEEEEEECCCCCCCEEEEEEECEECCCCCEEEEECCCCCCCHHHHHHH
KGCHSPDGGTVFAYEVSDPSRYGVVTFDATGTALSIEEKPAHPRSNYAVVGLYFYNNDVV
HCCCCCCCCEEEEEECCCCCCEEEEEECCCCCEEEECCCCCCCCCCEEEEEEEEECCCEE
RIAETTTPSARGELEITAINDAYLQQGKLHVRKLERGDVWLDTGTIDSMSEAASYVEVLQ
EEEECCCCCCCCEEEEEEECHHHHHCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHH
KRTGVIIGSPEVAAWKAGLITDTQLEALAQPLKKSGYGNYLLRTIRSAIP
HHCCEEEECCCHHEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9084178 [H]