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The map label for this gene is alkB [H]

Identifier: 38232761

GI number: 38232761

Start: 113144

End: 113884

Strand: Direct

Name: alkB [H]

Synonym: DIP0133

Alternate gene names: 38232761

Gene position: 113144-113884 (Clockwise)

Preceding gene: 38232758

Following gene: 38232762

Centisome position: 4.55

GC content: 59.65

Gene sequence:

>741_bases
GTGGCTGGGGTATGCGGGTCTAAGCTCATGATGGCTAGGTTAGTGGGTATGCCAGATCTTTTCAGTGATAGCGGTTGGGG
TAGTGGTGAGCTGCCACGGGCTCTGCGCCCTGGTCTTGTTCACCTGCCACGTTGGATGGGGTTGGACCAGCAGTTCGCCG
TGGTGCAGCAGTGCCGTGAGATTGCGCGCTCGGTGGCAGGTACTCCGCTTGCGATGCACCGGCAGCAGTGGGCGTCTGGC
ACGATGAGTGCCTATTTGATGTCCTTGGGGTTGCACTGGGAATACCGCACCTACCAGTATGTTTCTCAGTGGGGTGGGGT
GGCTGTCCCGCCTATTCCTGTGGAGTTTTCGGCGCTGGCGCATGAGGTTTTGCGGGCTGCTGCGGGTGTTGACGACTCCC
TTGCCGCGTGGGTGGATTCTTATCGCATTGATGCGGCTTTGGTGAATTATTATCCGCCGGGTGCTGGGATGGGTATGCAT
CAAGATGCGTTTGAGGATTCCCGTGCGCCGGTGGTGTCGTTGTCTATTGGAGATTCGGCGGTGTTTCGGGCGGGCAATGG
CGTGAATCGGCAGCGTCCGTGGCAGGACGTGGTGTTGGGGAGCGGGGATGCGGTGGTGTTTGGTGGACCGTCGCGAGACA
TGTTTCATTCGGTGGTGCGGCTGCACGCGGGCACGGCACCGACGCGATGTGGGGTGTCTCAGGGGCGGATAAATCTGACA
TTTCGGCAGGTAAAGCTATAG

Upstream 100 bases:

>100_bases
GGCGTTGTAATCGAAAACCAACTCGGCGGTACGAGCCATAGCCTGCGTGATCTCCGGAGATACCGGGATATGCCATCGTT
GGGCGCGCTGGTCGGCCATC

Downstream 100 bases:

>100_bases
CTTCCCCCTGTTTTACCCCCGCGTGTTGGAGGGATGTTTTAGCCTCGCTGTTATGAACGGTATGGATCTTATTTCTTGTG
ATGATGGTCGGGTACGCCCT

Product: putative DNA repair protein

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MAGVCGSKLMMARLVGMPDLFSDSGWGSGELPRALRPGLVHLPRWMGLDQQFAVVQQCREIARSVAGTPLAMHRQQWASG
TMSAYLMSLGLHWEYRTYQYVSQWGGVAVPPIPVEFSALAHEVLRAAAGVDDSLAAWVDSYRIDAALVNYYPPGAGMGMH
QDAFEDSRAPVVSLSIGDSAVFRAGNGVNRQRPWQDVVLGSGDAVVFGGPSRDMFHSVVRLHAGTAPTRCGVSQGRINLT
FRQVKL

Sequences:

>Translated_246_residues
MAGVCGSKLMMARLVGMPDLFSDSGWGSGELPRALRPGLVHLPRWMGLDQQFAVVQQCREIARSVAGTPLAMHRQQWASG
TMSAYLMSLGLHWEYRTYQYVSQWGGVAVPPIPVEFSALAHEVLRAAAGVDDSLAAWVDSYRIDAALVNYYPPGAGMGMH
QDAFEDSRAPVVSLSIGDSAVFRAGNGVNRQRPWQDVVLGSGDAVVFGGPSRDMFHSVVRLHAGTAPTRCGVSQGRINLT
FRQVKL
>Mature_245_residues
AGVCGSKLMMARLVGMPDLFSDSGWGSGELPRALRPGLVHLPRWMGLDQQFAVVQQCREIARSVAGTPLAMHRQQWASGT
MSAYLMSLGLHWEYRTYQYVSQWGGVAVPPIPVEFSALAHEVLRAAAGVDDSLAAWVDSYRIDAALVNYYPPGAGMGMHQ
DAFEDSRAPVVSLSIGDSAVFRAGNGVNRQRPWQDVVLGSGDAVVFGGPSRDMFHSVVRLHAGTAPTRCGVSQGRINLTF
RQVKL

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Escherichia coli, GI1788541, Length=199, Percent_Identity=34.1708542713568, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 26658; Mature: 26527

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGVCGSKLMMARLVGMPDLFSDSGWGSGELPRALRPGLVHLPRWMGLDQQFAVVQQCRE
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHCCCCHHHHHHHHHHH
IARSVAGTPLAMHRQQWASGTMSAYLMSLGLHWEYRTYQYVSQWGGVAVPPIPVEFSALA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHH
HEVLRAAAGVDDSLAAWVDSYRIDAALVNYYPPGAGMGMHQDAFEDSRAPVVSLSIGDSA
HHHHHHHCCCCHHHHHHHHHHHHCEEEEEEECCCCCCCCCCHHHHCCCCCEEEEECCCHH
VFRAGNGVNRQRPWQDVVLGSGDAVVFGGPSRDMFHSVVRLHAGTAPTRCGVSQGRINLT
EEECCCCCCCCCCHHHHEECCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
FRQVKL
EEEECC
>Mature Secondary Structure 
AGVCGSKLMMARLVGMPDLFSDSGWGSGELPRALRPGLVHLPRWMGLDQQFAVVQQCRE
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHCCCCHHHHHHHHHHH
IARSVAGTPLAMHRQQWASGTMSAYLMSLGLHWEYRTYQYVSQWGGVAVPPIPVEFSALA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHH
HEVLRAAAGVDDSLAAWVDSYRIDAALVNYYPPGAGMGMHQDAFEDSRAPVVSLSIGDSA
HHHHHHHCCCCHHHHHHHHHHHHCEEEEEEECCCCCCCCCCHHHHCCCCCEEEEECCCHH
VFRAGNGVNRQRPWQDVVLGSGDAVVFGGPSRDMFHSVVRLHAGTAPTRCGVSQGRINLT
EEECCCCCCCCCCHHHHEECCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
FRQVKL
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 1904855 [H]